the role of actin in the cell

... whether the actin cytoskeleton acts as a node of convergence in BR and auxin signaling We show that, in Arabidopsis, BR alters the actin cytoskeleton in an auxin-like manner by unbundling actin ... eval-uated the effect of BR on actin cytoskeleton configuration In vivo F-actin imaging was performed on roots of Arabidopsis seed-lings, using transgenic plants expressing the second actin-binding ... integration of the BRsignaling pathway and auxin responsiveness, functioning as a node of interaction of these two hormone pathways. Auxin transport inhibitors bundle actin microfilaments, re-stricting

Ngày tải lên: 30/04/2024, 00:04

... evolution They play key indispensable role in development PAX proteins are defined by the presence of an 128 amino acid DNA binding domain at their amino terminal end referred to as the,‘Paired Domain’, ... regulates the transcription of MET in SCLC We therefore investigated further the role of PAX8 in NSCLC Under conditions of normal development, PAX8 is expressed in the thyroid, kidneys, some part of ... manually using a conven-tional four-point pathology scoring system (0 - no staining, 1 - weak staining, 2 - moderate staining and 3 - strong staining), and then by an Automated Cellular Imaging System

Ngày tải lên: 05/11/2020, 00:46

... testis The CCDC6 protein was widely expressed in the germinal epithelium, mostly in the spermatogonial cells found at the basal compartment of the seminiferous epithelium, where they adhere to the ... antibody The representative immunohisto-chemical stainings are shown in Figure 3 CCDC6 was Trang 7virtually undetectable (<2.0% of cells) in the nuclei of theneoplastic cells in the seminomas In the ... loading control. Trang 10an alarming rise in the industrialized countries Thistrend, together with the worrying decline in male repro-ductive health, and the fascinating biology of the germ cells

Ngày tải lên: 05/11/2020, 05:05

... cell lines [10] Evidence has shown that the coding sequence of the brevican gene in glioma is the same as the brevican gene found in the cortex of the normal brain Therefore, the regulation of ... diameters of the tumor In addition, 2×105 cells were intracranially injected into the right thalamus of BALB/c nude mice using a 10μl syringe A total volume of 8 μl cell suspension was injected ... tumor of the central nervous system Brevican, an abundant extracellular matrix component in the adult brain, plays a critical role in the process of glioma The mechanisms for the highly invasive

Ngày tải lên: 05/11/2020, 08:08

... p 33) Another striking feature of the transition was that the fall in the official GDP coincided with the rise of an informal economy If the latter were included into the accounting of GDP that ... investment in education in these post-communist countries The first chapter analyzes the determinants of economic growth in these countries and focuses on the role of investment in education in the ... communist ideology In order to fulfill these goals, the communist leadership early on recognized the important role of education in the process of indoctrination and in the formation of these new communist

Ngày tải lên: 18/02/2014, 11:20

... which appear to be involved in the final resorption of the parental macronucleus during PND in T thermophila, and suggested the involvement of mitochondria in this process [19] In mammalian apop-tosis, ... than in the production of large-sized DNA fragments, considering the timing of uptake of the mitochondria in the autophagosome, as discussed below Mitochondria as a possible executor of PND The ... According to the observation made by Lu and Wolfe [23], who used a combination strategy of 4,6-diamino-2-phenylindole (DAPI) staining for the detection of DNA and Azo dye staining for the identification

Ngày tải lên: 20/02/2014, 03:20

... Aning, Turkson, and Asuming-Brempong 2008; Obi, Oparinde, and Maina 2008; Omiti and Okuthe 2008) Therefore, information regarding the role of poultry in the livelihoods of small-scale producing ... detail the role of poultry (both as a source of income and as an asset) in the entirety of the households’ income and asset portfolios Regarding the sources of data used in this study, for the ... culled No information is available on the costs of culling, diagnostic testing of samples, cleaning and disinfection, and other administrative costs (Obi, Oparinde, and Maina 2008) Regarding the impacts

Ngày tải lên: 21/02/2014, 01:20

... direction The inverse is the case for the attraction in the case of Anabaena Also, if the structure of the final complex in which electron transfer takes place remained unaltered with increasing ionic ... plastocyanins has been described [1] Representations of the electrostatic surfaces showing the changes introduced by the mutations are displayed in Fig 1 The decay of the flash-induced absorbance of ... laminosum and spinach The response to ionic strength of the reaction between P laminosum plastocyanin and photosystem I was in marked contrast with the behaviour of the homologous system in spinach

Ngày tải lên: 21/02/2014, 01:21

... FUNCTIONSlet L4 be the quantity of input of the Ith labor service, measured in hours The rate of growth of the index of total labor input, say L, is: man-i — L where v1 is the relative share of the lth ... number of men and e1 the proportion of the workers in the lthcategory of labor services, one may write the index of the total labor input in the form: = — +—+ —. Thus, to eliminate errors of aggregation ... a hypothesis,partly because of lack of relevant data They used the education of the mea-total labor force in a state for the measurement of the quality of the labor force of individual industries

Ngày tải lên: 06/03/2014, 21:20

... some hint of partial participation in a subset of the metal com-plexes present in our preparations The role of histi-dine in metal ion coordination in MTs is a subject that has gathered increasing ... CeMT1 The presence of histidines in these MTs is revealed to be decisive for their coordination performance In CeMT1, they contrib-ute to the binding of a seventh Zn(II) ion in relation to the ... coordination of the Zn(II) ion bound at the C-terminal moiety of CeMT1 is con-tributed to by histidines, and the number of these involved in metal binding is analyzed below Thus, the overall results

Ngày tải lên: 07/03/2014, 02:20

... bromodomain-bearing proteins, enabling them to bind acetylated chromatin (Fig 3) Bromodomains are acetyl-lysine binding modules present in ATP-dependent chromatin remodelling factors as well as in ... phophorylation of Thr/Ser in Thr/Ser-Lys or Lys-Thr/Ser pairs found in the four histones may negatively regulate the binding of chromodomains to methylated lysines Indeed, chromo-domain-containing proteins ... existence of a machinery capable of specifically recognizing and reading the histone code The existence of cellular factors recogni-zing and binding to specifically modified histones is in support of this

Ngày tải lên: 07/03/2014, 16:20

... analyzed the role of gangliosides in the association of ErbB2 with lipid rafts in mammary epithelial HC11 cells The key role played by gangliosides in defining the distribution of ErbB2 into signaling ... fractions in HC11 cells Because in cells coexpressing both ErbB2 and EGFR, as is the case of HC11 cells, the two proteins strictly interact and EGF preferentially stimulates the formation of ErbB2 ... specifically interact with the purified recombinant extracellular domain of EGFR [22] and that this tyrosine kinase receptor contains a structural domain with targeting information for lipid domains [23]...

Ngày tải lên: 23/03/2014, 10:21

... on the regulation of the IP-10 secretion The aim was to investigate the pathways of IP10 secretion in a in vitro system including the cell types most likely involved in the IP-10 secretion in the ... distinct soluble factors governing the IP-10 response in endothelial versus epithelial cells The secreted factor from lung epithelial cells might be a growth factor, interleukin or interferon, since ... studies To further probe the signalling pathways involved in modulating IP-10 expression in the epithelial cell/ PBMC co-cultures, we investigated the pharmacological effect of a number of signal...

Ngày tải lên: 12/08/2014, 15:21

... apoptosis in endothelium of intestine, lung, fat and thymus Interestingly, the apoptosis was inhibited in the endothelium by administration of TNF-binding protein, a protective factor against LPS-induced ... in the de novo ceramide synthesis pathway in rat lung epithelial cells 84 Figure Effect of CS exposure on the activity of enzymes in the sphingomyelinase pathway in rat lung epithelial cells ... endothelial and epithelial cells in vitro Overproduction and accumulation of ceramides are involved in the alterations of the lung parenchyma induced by CS, which culminate in the development of...

Ngày tải lên: 24/08/2014, 09:42

... cell in a state of dormancy or inactivity in which the cell is not dividing, therefore is in G0 phase of the cell cycle Quiescence should not be misinterpreted with other processes in which cells ... 1 AN INVESTIGATION INTO THE ROLE OF CHEMOKINES IN HAEMOPOIETIC STEM CELL QUIESCENCE Amy Sinclair BSc (hons), MRes Submitted in fulfilment of the requirements for the degree of Doctor of Philosophy ... conclusions In order to gain a deeper understanding of the biological function of chemokine signalling in HSC biology, we used an in vivo murine system First, we examined mRNA transcripts of CXC chemokines...

Ngày tải lên: 22/12/2014, 21:11

... Evaluation of Human SPHK Inhibitors visible color change Within the linear range of the assay (~5-25μg/mL), the more protein present, the more the Coomassie Blue binds changing the absorbance of the ... of Human SPHK Inhibitors components in the samples, which are then separated in the TLC plate The plate was run in the chamber until the solvent front reached 2cm from the top of the plate, then ... was not in the final list and not being tested further, is mainly because the yield of compound 2a3 is really low (~7%) Therefore, obtaining compound 3a3 requied a large amount of the starting material-Garner’s...

Ngày tải lên: 11/09/2015, 16:06

... Staining 3.1.3.1 Alizarin Red S Staining Alizarin Red S staining is widely used to evaluate the calcium-rich deposits generated by cells In this study, the staining was used to detect the mineralization ... to be the best inhibitor candidate, in terms of the ability to penetrate into the cells and inhibit the endogenous SPHK1 and the good yield obtained from the chemical synthesis procedure Therefore, ... vs cock) However, in cells cultured in the presence of 1μM of DMS, 1μM of CP6, and 5μM of CP6, there was no increase in osteocalcin expression, compared the cells grown without inhibitors (cock+1...

Ngày tải lên: 11/09/2015, 16:06

... were counted The percentages of the expressed cell surface markers in the cells are summarized in Table 4.1, and plots of receptor expression of the last passage cells cultured in two different ... for a total of 28 days, then fixed and stained with Alizarin Red S The Alizarin Red S Staining quantification results are shown in Figure 4.11 Alizarin Red Staining quantification of human BM-MSCs ... the presence of S1P did not show any side-effect on maintaining stemness of the cells However, the amount of cells that were proliferated in the S1P serum-free, or S1PPDGF-AB combined culture...

Ngày tải lên: 11/09/2015, 16:06

... et al (1998) “N,N-Dimethylsphingosine Is a Potent Competitive Inhibitor of Sphingosine Kinase but Not of Protein Kinase C: Modulation of Cellular Levels of Sphingosine 1-Phosphate and Ceramide.” ... “Characterization of N, N-dimethyl-derythro-sphingosine-induced apoptosis and signaling in U937 cells: independence of sphingosine kinase inhibition.” Prostaglandins & Other Lipid Mediators 86(1-4):18-25 ... "Signalling mechanisms in sphingosine 1-phosphatepromoted mesangial cell proliferation." Genes Cells 7(12): 1217-30 Kee, T H., P Vit, et al (2005) "Sphingosine kinase signalling in immune cells." Clin...

Ngày tải lên: 11/09/2015, 16:06

... increasing need for more MSCs is one of the main motivations of the present study, in particular, in studying the role of S1P in adult stem cell proliferation Another major motivation is the pressing ... activation, while HDL could inhibit all of these, by inhibiting the SPHK activity triggered by TNFα These findings suggested an atherogenic role for SPHK activation Interestingly, Nofer et al (2004) reported ... stimulate their growth and maintain of their multipotency, which limits the development of the strategies for stem cell expansion in better controlled, serum-free, conditions In this study, the role of...

Ngày tải lên: 11/09/2015, 16:06