the involvement of ogt in apoptosis

... contain the entire coding region Thus, use of alternative promoters in DNASE1 results in no generation of protein isoforms The 5¢-UTR of eukaryotic mRNA in uences the initiation step of protein ... the cycle number at which the fluorescence of the reporter dye crossed the threshold in log-linear range of PCR The copy numbers of the respective DNASE1 cDNA were quantified by interpolating the ... corresponding to the DNASE1 cDNAs starting from either exon 1a or exon 1, respectively, into COS-7 cells and then determined the levels of DNase I activity secreted into the medium of the cells...

Ngày tải lên: 19/02/2014, 06:20

... involved in the biosynthesis of natural polyamines (putrescine, spermidine and spermine) through conversion of l-arginine into l-ornithine [16] The latter is in turn used by ODC to produce putrescine, ... iodide (PI) staining of apoptotic cells was quantified in VSVinfected Jurkat T cells in the absence or presence of spermine As shown in Fig 7, the presence of spermine during VSV infection strongly ... mitochondrial enzyme involved in the polyamine synthesis pathway through the catalysis of l-ornithine production from l-arginine Of the natural polyamines, spermine and to a lesser extent spermidine, possess...

Ngày tải lên: 07/03/2014, 21:20

... http://www.virologyj.com/content/2/1/1 Inhibition of PI3K results in an increase in the speed and magnitude of RV-induced apoptosis To evaluate the role of PI3K-dependent signaling during RV infection, the effects of PI3K inhibitor ... 3A) This increase in speed and magnitude of RV-induced apoptosis is more strikingly observed in Fig 3B, which shows the number of dead floating cells by trypan exclusion staining in the culture ... were in tact with no visible cytotoxicity (Fig 3D) Inhibition of MEK1/2 reduces RV-induced apoptosis The role of Ras-Raf-MEK-ERK signaling in RV-induced apoptosis was investigated using MEK inhibitor...

Ngày tải lên: 18/06/2014, 22:20

... hybridizes to the center of the input target RNA The asterisk indicates the input target RNA; the arrow indicates the digested product bands The amount of digested product obtained is indicated at the ... freezing or diluting of the extracts The extracts used in one experiment were always isolated at the same time and treated in the same way So within one experiment, the ratio of the extracts of ... only in in-gel assays, the class II activity of RNH(35) only in liquid assays, whereas the class II activity of RNH(70) was detected in neither assay In order to determine the contribution of the...

Ngày tải lên: 08/03/2014, 10:20

... because of the attachment of the hexahistidine, the recombinant PpTGase protein appeared to be slightly larger These results indicate that the cDNA obtained covered the entire coding region Involvement ... form Initially, determination of the amino-terminal amino acid sequence of purified protein was attempted, but no information was obtained, probably due to the protein modification Therefore, the ... terminus that is required for binding to the plasma membrane, thereby being involved in the formation of the corneum [47] Additional amino-terminal residues of TGase include the sequences that are post-translationally...

Ngày tải lên: 17/03/2014, 23:20

... involved in the regulation of recovery rather than in the initiation of the disease In addition, CCL19 and CCL21 are expressed by endothelial cells of the BBB, and are involved in the strengthening ... of the murine model provide further insights in the characterization of CCL / CCR molecules in EAE In mice with the CCL2 transgene under the control of the lck (which directs the expression of ... the involvement of chemokines in the integrin-mediated arrest of autoreactive T cells at the BBB, the investigators [3] subsequently aimed to identify the specific chemokines by performing in...

Ngày tải lên: 19/06/2014, 22:20

... impairment in order to understand the mechanism of action of betaine in the central nervous system In this study, we investigated the effects of betaine on LPS-induced memory impairment using the Y-maze ... the expression of proinflammatory molecules and increased oxidative stress in aged rat kidney [6, 7] This finding indicates that the mechanism behind the actions of betaine in the central nervous ... J, Iversen LL: Regional studies of catecholamines in the rat brain I The disposition of [3H]norepinephrine, [3H]dopamine and [3H]dopa in various regions of the brain J Neurochem 1966, 13:655-669...

Ngày tải lên: 19/06/2014, 22:20

... expression of any of the Bax isoforms in the mockinfected SIRC cells (Fig 4) Interestingly, we observed a dramatic rise in the level of Bax-β in HSV-1-infected cultures (Fig 4) Following the demonstration ... profound alterations in the viability of the ocular cells and in the tissue homeostasis of the ocular surface As a step in our investigations of the underlying molecular events implicated in ... replication To investigate the basis of the HSV-1-induced increase of the TAp63γ level, SIRC cells were infected in the presence or absence of the viral DNA replication inhibitor ACG The cells were...

Ngày tải lên: 10/08/2014, 05:21

... induce apoptosis of glioma [21] Since STAT3 is activated by cytokine receptor-associated tyrosine kinases or growth factor receptor intrinsic tyrosine kinases, besides antagonizing the function of ... role in the deregulated proliferation and uninhibited growth of brain tumors [36] STAT3 activation by bFGF-FGFR binding has been implicated in the regulation of JAK2 and Src kinase activities in ... Mechanism Involving Platelet-activating Factor, JAK-2, and Src in Human Umbilical Vein Endothelial Cells JBC 2002, 277:21237-21245 Chan SL, Yu VC: Proteins of the bcl-2 family in apoptosis signaling:...

Ngày tải lên: 10/08/2014, 10:21

... changes in practice The recognition of the failure of this model has led to greater awareness of the role of other factors in the practice environment influencing behaviour [1] and the importance of ... Discussion In this study, we have used two different 'off -the- shelf' methods of identifying opinion leaders across a range of different professional groups in the UK The study utilised existing instruments ... professional groups and sites in the extent of nominating SOLs and the complexity of networks Furthermore, this has been one of the first studies to examine whether opinion leaders are polymorphic...

Ngày tải lên: 11/08/2014, 05:21

... was partly due to induction of the gene in shoots GUS staining pattern of NaCl-treated roots seemed to be more complex GUS staining was indeed sharply reinforced in the terminal Renault et al ... white boxes visible in B and C Arrows point to primary root nutrition of plant suggesting involvement of this isoform in nitrogen metabolism [37] Therefore, the increase of GAD2 expression at ... mM NaCl before GUS staining (A) GUS staining pattern in shoots of plantlets (B-C) GUS staining pattern in roots of plantlets shown in A (D-E) Focus on root apices visible in B and C (F-G) Focus...

Ngày tải lên: 12/08/2014, 03:21

... grown in the dark to examine how the mutations affected phenanthreneinduced growth responses in seedlings The mutants included the ethylene overproducer eto3 [32]; the ethylene-insensitive gain -of- function ... cytoplasmic concentration of PAHs, thereby decreasing the effective toxicity to the plant and allowing increased uptake of the pollutant For example, further increasing GST and UGT protein levels, or artificially ... gene expression in both lines when grown in long days in the presence of phenanthrene In both lines, the reporter expression occurred in scattered patches on the leaf blades These spatial patterns...

Ngày tải lên: 12/08/2014, 03:21

... throughout the molecule (Kumar et al., 1998a; Yu et al., 1995) The COOH-terminus of kinectin consists of two functional domains The kinesin-binding domain can interact with the cargo-binding site of the ... characterize the EF-1δ interacting domain on kinectin 2.4 To investigate the effect of kinectin in the in vitro translation assay 2.5 To investigate the co-localization of kinectin with EF-1δ by immunofluorescence ... indicating that kinectin can bind kinesin Firstly, kinectin is one of the most abundant vesicle proteins that bind to the SUK4-kinesin column In microsomal extracts of fresh chicken brain, kinectin...

Ngày tải lên: 12/09/2015, 10:00

... 27 The effects of EDTA on WNV and JEV binding to Vero cells 139 Figure 28 Sequencing result of pSilencer-INTB3 showing the presence of human β3 integrin siRNA sequence (in red and underlined) inserted ... Damages are seen in the meninges contributing to meningitis, the brain parenchyma contributing to encephalitis and the spinal cord contributing to myelitis In the recent outbreak of WNV in North America, ... 1987) and instead terminate with the conserved dinucleotide CUOH The most notable feature of WNV genome is the presence of one ORF of over 10,000 bases The order of the proteins encoded in the long...

Ngày tải lên: 16/09/2015, 17:13

... aimed to investigate the involvement of RSV-induced ROS production in regulating NOX5 expression in the U937 lymphoma cells The initial part of the study involved analyzing the expression of NOX5 ... of selenium-containing enzymes involved in the removal of H2O2 via the coupling of its reduction to the oxidation of glutathione (GSH) GSH is a thiol-containing tripeptide The oxidized form of ... calmodulin (CaM), a calcium binding protein also regulates the activity of NOX5 CaM binding domains were found on the C-terminus rather than the EF domains which are known to function as Ca2+ binding...

Ngày tải lên: 26/09/2015, 10:44

... or lying on the ground) with all the vertical poles diminishing in size as they proceed along the limiting lines of perspective towards a central vanishing point lying in the centre of the horizon ... end of your picture Start with the outline The colour could be ultramarine but a fairly watery mix Then define all the blocks of architecture and the line of the base of the buildings where they ... and interest to the details of the buildings Cover the areas of distant vegetation with a green tone Then with a much deeper version of the original colour of the canal, put in the reflection of...

Ngày tải lên: 13/09/2012, 14:19

... these values, could be contained The emotional intensity of the backlash against the intrusions of these subaltern groups suggests the importance of reading these developments in ways which interfuse ... advances in the print industry.22 In Letters on Education (1790), Catherine Macaulay argued that the ‘advantages of printing, by rendering easy the communication of ideas, giving an universality to their ... demonstrates, they are none the less able to inscribe themselves within it, and in doing so, to gain a limited measure of autonomy without necessarily reproducing the myth of the selfdetermining subject.31...

Ngày tải lên: 21/09/2012, 11:00

... profitability is declining The internalization of information within the same institution may thus enhance intra-circuit and inter-circuit stability by reducing the incentive to overfinancing The ... Creating a stronger investor base amounts to strengthening the circuit by completing the financial market structure of the economy, thus eliminating discontinuities in the transmission of information ... placing money with investment financial institutions in the capital market, and hold the remaining share in noninterest bearing, short-term bank deposits (eq 7) The share of savings going to the...

Ngày tải lên: 24/10/2012, 09:33

... resulted in most of the mucin eluting in the included volume of the column, suggesting the findings of previous studies that the mucin sample could have had a fraction resistant to reduction [12] The ... of the purified mucin gave high amounts of ‘mucin-like amino acids, serine, threonine and proline Serine and threonine are points of O-glycosylation found in the tandem repeat regions of mucins ... confirming the specificity of the antibody Amino Acid Analysis The results are shown in Table with the signature amino acids for mucins, namely, serine, threonine and proline comprising 48.3% of the...

Ngày tải lên: 26/10/2012, 10:03