the interaction of intense femtosecond laser light with molecules

Báo cáo Y học: Biophysical characterization of the interaction of high-density lipoprotein (HDL) with endotoxins doc

Báo cáo Y học: Biophysical characterization of the interaction of high-density lipoprotein (HDL) with endotoxins doc

... necrosis factor-a production (Fig 10) The comparison of the results of the interaction of LPS with HDL to those published for the interaction of the former with another serum protein, albumin, indicates ... ratio of [LPS]/ [HDL] : (Fig 3) indicates the significant influence of the acyl chain moiety of LPS in the interaction which might be connected with the observation that the acyl chain melting in the ... model of the LPS– HDL interaction can be deduced The binding of HDL takes place essentially to the diglucosamine sugar backbone and the 4¢-phosphate of lipid A The binding-places within the HDL...

Ngày tải lên: 17/03/2014, 10:20

10 573 0
Characterization of the interaction of EEN and its domains with ca2+ and proline rich domain

Characterization of the interaction of EEN and its domains with ca2+ and proline rich domain

... the dimer by the FPLC because of the large molecular weight (Figure 4.1.3) In further, these polymers may accelerate the process of the EEN aggregation Therefore preventing the formation of the ... Chapter presents the results obtained, while Chapter discusses the contribution of these results on understanding of the possible role of the random coil PRD and the influence of Ca2+ on EEN in ... amphipathic helix, thereby extending the helical backbone of the dimer at the tips Together with the BAR domain, this sequence motif is termed as N-BAR and can be found in a subgroup of the BAR-domain...

Ngày tải lên: 03/10/2015, 20:32

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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx

Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx

... as a consequence of the mutagenic replacements Effects of the His120Asn and His145Asn mutations on the affinity of metal binding to arginase II To further examine the effects of the His120fiAsn and ... the interactions of arginase II with l-arginine and agmatine are greatly altered by replacement of this residue with aspartate To the best of our knowledge, this is the first report in which the ... being involved in the stabilization of the transition state [27], but not in the stabilization of the substrate in the Michaelis–Menten complex [24] Also in agreement with this, the Km value was...

Ngày tải lên: 20/02/2014, 02:21

9 653 0
Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt

Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt

... Consequently, the molecular studies of the interaction of SAgs with their specifics ligands will not only advance understanding of the physiological mechanisms of these molecules, but may lead to the development ... for the immobilized TCR Vb5.2 of wtSSA of 153 lM, which is near the lower limits of the known SAg–TCR interactions [26] To avoid any altered Kd determination due to the immobilization process of ... on the opposite face of the molecule that is Zn2+ dependent [14,15] The proinflamatory and procytotoxic properties of SAgs are responsible for the increased interest in these molecules in the...

Ngày tải lên: 07/03/2014, 16:20

9 485 0
Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot

Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot

... interacting with the protein, because the resonance signals of the glucose residue overlapping with those of the galactose residue within the lactose affected the subtraction of the free induction ... interacts with the region of H1 and the O-methyl group The crystal structure of EW29Ch with lactose showed that the O2, O3 and O4 atoms of the galactose residue of lactose formed hydrogen bonds with ... to that of the higher binding site by NMR, even if the sugar-binding ability of one of the two binding sites is much higher than that of the other However, the hemagglutinating activity of lectin...

Ngày tải lên: 23/03/2014, 04:21

11 459 0
Báo cáo khoa học: The influence of cholesterol on the interaction of HIV gp41 membrane proximal region-derived peptides with lipid bilayers potx

Báo cáo khoa học: The influence of cholesterol on the interaction of HIV gp41 membrane proximal region-derived peptides with lipid bilayers potx

... by the particular disposition of key residues in the context of long peptides or even in the context of the full protein Bearing this in mind, a MPRP-S role in the interaction of the MPR with ... about the interaction of peptides with large unilamellar vesicles (LUV) The fluorescence quantum yield is dependent on the polarity of the microenvironment of the Trp residues, as well as on the ... Castanho The membrane interaction behaviour of MPRP-C and MPRP-I is independent of the membrane phase but not of the presence of charged lipids Cholesterol does not reduce the extent of or potentiate...

Ngày tải lên: 23/03/2014, 07:20

9 286 0
Báo cáo khoa học: A spectroscopic study of the interaction of isoflavones with human serum albumin pdf

Báo cáo khoa học: A spectroscopic study of the interaction of isoflavones with human serum albumin pdf

... energetics The effect of temperature on the interaction of genistein with HSA has been followed in the range 17–47 °C The binding constant, K, exhibits a recipro- Fig Quantitation of the interaction of ... overlap of the emission spectrum of the donor with the absorption spectrum of the acceptor The energy transfer efficiency E is related to the distance (r0) between acceptor and donor, and also to the ... 27 °C Effect of ionic strength on binding of genistein– HSA interaction To determine whether ionic interactions play a role in the genistein–HSA interaction, the ionic strength of the buffer was...

Ngày tải lên: 23/03/2014, 11:20

17 457 0
Báo cáo khoa học: Site-directed mutagenesis and footprinting analysis of the interaction of the sunflower KNOX protein HAKN1 with DNA ppt

Báo cáo khoa học: Site-directed mutagenesis and footprinting analysis of the interaction of the sunflower KNOX protein HAKN1 with DNA ppt

... towards the 3¢ end of the top strand and the 5¢ end of the bottom strand (Fig 4B) This agrees with the presence of a larger residue that makes stable contacts with this region of DNA The interaction ... achieved The combination of these experiments with the footprinting results and previous 197 KNOX homeodomain–DNA interactions A B Fig A model for the interaction of HAKN1 with DNA (A) Diagram of the ... least one of the members of the CÆG pair at the fourth position of TGAC in the HAKN1–DNA complex The two leftmost positions of the core interact through the minor groove with the N-terminal arm in...

Ngày tải lên: 23/03/2014, 13:20

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Báo cáo khoa học: The interaction of the Escherichia coli protein SlyD with nickel ions illuminates the mechanism of regulation of its peptidyl-prolyl isomerase activity ppt

Báo cáo khoa học: The interaction of the Escherichia coli protein SlyD with nickel ions illuminates the mechanism of regulation of its peptidyl-prolyl isomerase activity ppt

... cluster that is the core of the domain The IF domain of SlyD is aligned with the IF domain of the archaeal homologous MtFKBP17 [32] (Fig 3) The main difference between these two domains is the longer ... hydrophobic interactions with the a1-helix, the b2-strand and the b3-strand, but it also establishes a few contacts with the interconnecting segments The structure of the PPIase module of SlyD closely ... helix connects to the rest of the domain through a well-dened segment extending from the b3-strand, and is positioned at the convex side of the b-sheet near to the ends of the b4-strand and b5b-strand...

Ngày tải lên: 30/03/2014, 01:20

16 233 0
Báo cáo Y học: Exploration of the diaphorase activity of neutrophil NADPH oxidase Critical assessment of the interaction of iodonitrotetrazolium with the oxidase redox components doc

Báo cáo Y học: Exploration of the diaphorase activity of neutrophil NADPH oxidase Critical assessment of the interaction of iodonitrotetrazolium with the oxidase redox components doc

... characteristic of the monoformazan, i.e the fully reduced product of INT [15,17] In the following aeration of the medium, the absorbance of the spectral bands decreased, but the shape of the spectrum ... inward The rates of oxygen uptake were deduced from the slopes of the tangents to the oxygraphic traces, and the contact points of the tangents with the curves were used to determine the O2 concentrations ... [13], either by measurement of the rate of production of O2– or the rate of O2 uptake INT diaphorase activity was assayed by the rate of reduction of INT into formazan in the presence of superoxide...

Ngày tải lên: 31/03/2014, 21:21

10 434 0
Báo cáo Y học: Tryptophan fluorescence study of the interaction of penetratin peptides with model membranes pdf

Báo cáo Y học: Tryptophan fluorescence study of the interaction of penetratin peptides with model membranes pdf

... internalization [12] The aim of this study was to gain better insight into the mode of interaction of the penetratin peptide with lipid bilayers and to investigate the role of the Trp residues and the lipids ... from the quenching of the intrinsic Trp uorescence intensity of the peptides, upon addition of SUVs The uorescence intensity at 350 nm, expressed as the percentage of the uorescence of the lipid-free ... decrease of the mean lifetime of the Trp residue(s) in the W56F- and WT- or W48F-penetratin The decrease of the mean Trp lifetime for the three peptides might account for the decrease of the Trp...

Ngày tải lên: 31/03/2014, 23:20

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Báo cáo y học: "HIV-1 integrase modulates the interaction of the HIV-1 cellular cofactor LEDGF/p75 with chromati" pptx

Báo cáo y học: "HIV-1 integrase modulates the interaction of the HIV-1 cellular cofactor LEDGF/p75 with chromati" pptx

... [33] The surface of interaction of the IBD with a subset of these proteins partially overlaps with that of integrase (reviewed in [19]) The affinity of IBD for integrase is higher than for some of ... accumulate in the cytoplasmic compartment of the transfected mouse fibroblasts as a result of the saturation of the nuclear import pathway responsible for the nuclear localization of these mutants ... chromatin and with HIV-1 integrase [1,2] To determine the effect of the ionic strength on the interaction of HIV-1 integrase with chromatin bound LEDGF/p75, we evaluated the consequence of increasing...

Ngày tải lên: 13/08/2014, 01:20

14 183 0
Effect of cationic micelles on the kinetics of interaction of Cr(III) gly gly2+ with ninhydrin

Effect of cationic micelles on the kinetics of interaction of Cr(III) gly gly2+ with ninhydrin

... stabilization of the transition state owing to the presence of micelles that facilitate the occurrence of the reaction The observed large decrease in ΔS# further strengthens the point The ΔH# and ... Fig.5 Plots of k versus [ninhydrin]T for the interaction of Effect of inorganic salts on the reaction rate for [Cr(III)-Gly-Gly] with ninhydrin in the absence (A) and the interaction of [Cr(III)-Gly-Gly]2+ ... a consequence of the adsorption of hydrophobic anion at the micellar surface and exclusion of substrate from the micellar surface The progressive withdrawal of the substrate from the reaction...

Ngày tải lên: 02/09/2015, 13:12

8 394 0
Interaction of a strongly focused light beam with single atoms

Interaction of a strongly focused light beam with single atoms

... for the interaction of the atom with the environment, and the spontaneous decay rate in free space Γ = Γ + Γp is made up of the sum of the decay into the environment Γ and the pulse mode Γp The ... weak coherent laser beam and the other using strong coherent pulses The outline of this thesis is as follows Chapter  presents the theory of light- atom interaction The simple case of a plane wave ... intensity of the dipole beam at the trap and δ is its detuning from the D line of 87 Rb The scattering rate of the beam by the atom on the other hand is proportional to I /δ2 For the experiment, the...

Ngày tải lên: 09/09/2015, 17:54

117 406 0
Investigation of the interaction of antimicrobial peptides with lipids and lipid membranes 1

Investigation of the interaction of antimicrobial peptides with lipids and lipid membranes 1

... formed upon interaction with micelles The region which is rich in tryptophan in the middle of the peptides interacts with one layer of the membrane and the two termini are oriented toward the aqueous ... role in the activity of antimicrobial peptides The one cysteine of the S3 peptide which is derived from the Sushi domain of Factor C allows the formation of S3 dimers The S3 dimer is capable of disrupting ... is also related to the L- or D- enantiomer However the effect of enantiomer of the amino acid is dependent on whether the replacement affects the structure of the peptide and the amphipathicity...

Ngày tải lên: 14/09/2015, 21:54

92 342 0
Investigation of the interaction of antimicrobial peptides with lipids and lipid membranes 3

Investigation of the interaction of antimicrobial peptides with lipids and lipid membranes 3

... value The presence of V4 further increased the compressibility of the monolayer, indicative of the increased molecular area, which was consistent with the result of excess surface pressure 154 The ... disruption The direct observation of the kinetics of these processes would greatly enhance the understanding of the mechanism of V4membrane interactions In the future, a possible method to address these ... One of the possible reasons might be the conformational change due to the interaction between V4 and lipid When there was no V4, the alkyl chains of the lipid molecules extended upward to the...

Ngày tải lên: 14/09/2015, 23:32

69 335 0
Investigation of the interaction of antimicrobial peptides with lipids and lipid membranes 2

Investigation of the interaction of antimicrobial peptides with lipids and lipid membranes 2

... amplitude of the ACF is inversely proportional to the particle number N However, if the fluorescence yields of the two species are not the same, the fluorescence yield of the particles will affect the ... presence of melittin overlapped well with that of labeled POPG LUVs At a peptide/lipid ratio of 1:8, there was a slight increase in the amplitude of the ACF and in the diffusion time When the peptide/lipid ... overlapped with that of labeled DPPG LUVs When the peptide/lipid ratio increased to 1:20, the amplitude of the ACF rose and the diffusion time increased compared with DPPG vesicles The amplitude of the...

Ngày tải lên: 14/09/2015, 23:32

63 187 0
A study on the techniques for the improvement to the teaching of oral skills in light of communicative english language teaching for junior high school teachers in quang ngai province part 1

A study on the techniques for the improvement to the teaching of oral skills in light of communicative english language teaching for junior high school teachers in quang ngai province part 1

... the aim of the lesson clear? Are the stages of the lesson clears? Does the teacher a variety of activities? Does the teacher encourage real use of language? Does the T use a warmer to begin the ... ORIGINAL STUCTURE: How often you | go | to the zoo? Introduce pictures of the park, the sports center, the mountain, the river, the kitchen and the school Check if the students know the vocabulary Set ... than the ENGLISH textbook series They like the clear layout, the variety of exercise types and the logical sequencing of the TIENG ANH textbook series However, they want to reduce the number of...

Ngày tải lên: 07/11/2012, 14:41

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A study on the techniques for the improvement to the teaching of oral skills in light of communicative english language teaching for junior high school teachers in quang ngai province part  2

A study on the techniques for the improvement to the teaching of oral skills in light of communicative english language teaching for junior high school teachers in quang ngai province part 2

... UNIVERSITY, HANOI COLLEGE OF FOREIGN LANGUAGES POST GRADUATE STUDIES DEPARTMENT TRẦN QUỐC VIỆT A STUDY ON THE TECHNIQUES FOR THE IMPROVEMENT TO THE TEACHING OF ORAL SKILLS IN LIGHT OF COMMUNICATIVE ENGLISH ... THỦ THUẬT NÂNG CAO VIỆC DẠY KỸ NĂNG NGHE NÓI TIẾNG ANH THEO ĐƯỜNG HƯỚNG GIAO TIẾP CHO GIÁO VIÊN TRUNG HỌC CƠ SỞ TẠI TỈNH QUẢNG NGÃI) M.A.THESIS HA NOI-2007 ...

Ngày tải lên: 07/11/2012, 14:41

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A study on the techniques for the improvement to the teaching of oral skills in light of communicative english language teaching for junior high school teachers in quang ngai province part  3

A study on the techniques for the improvement to the teaching of oral skills in light of communicative english language teaching for junior high school teachers in quang ngai province part 3

... National University-Hanoi for their valuable lectures And their knowledge, their thoughtfulness as well as their sympathy I will always appreciate For the accomplishment of this study, I wish to show ... like to acknowledge my debt to Assoc Prof Dr Lê Hùng Tiến and the staff members of Postgraduate Department, College of Foreign Languages, VNU-Hanoi for their enthusiastic support I am sincerely ... Teachers' workload 11 • Professional support 11 • Teachers' responses to the new Tieng Anh 6-9 textbook series .11 • Teachers' perception of their own teaching 11 2.6.2.5...

Ngày tải lên: 07/11/2012, 14:41

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