thawing of cell lines from frozen stocks

... (h) D 100 80 HEL cells 120 72 HEp-2 cells 120 Cell viability (% of control) 100 C Cell viability (% of control) B HaCaTcells 120 Cell viability (% of control) Cell viability (% of control) A 72 ... cytotoxicity against some human cell lines, such as human keratinocytes (HaCaT cells), human embryo lung fibroblast cells (HEL cells), human nasopharyngeal carcinoma cells (HEp-2 cells) and PMNs in vitro ... SEM from three independent experiments (A) HaCaT cells were incubated with 100 lM each organic selenium compound Cell viability was measured at intervals of 24 h (B) Cell viability of HEL cells...

Ngày tải lên: 23/03/2014, 07:20

... characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines Journal of Translational ... expression of myeloid suppressor cells and tumor cell lines For gene expression studies, tumor-educated CD33+ or CD11b + cells were isolated from tumor-PBMC cocultures by fluorescence activated cell ... fresh, autologous T cells in the presence of T cell stimuli Use of irradiated tumor cells in cocultures yielded comparable suppressor cell induction, suggesting that tumor cells need not be actively...

Ngày tải lên: 18/06/2014, 19:20

... of cell lines Cell lines Cell culture Zpl 2-1 and C6 cell lines were used for the neuronal cell marker and the glial cell marker, respectively The neuronal cell line Zpl 2-1 was established from ... Figure Characterization of morphology of established cell lines Characterization of morphology of established cell lines Morphological appearance of the R1A, P8A, and ICR-A cell lines were compared ... P8A cell lines than in R1A cell Figure Immunoblot analysis of CAgag in culture media from ICR-A, R1A and P8A cell lines Immunoblot analysis of CAgag in culture media from ICR-A, R1A and P8A cell...

Ngày tải lên: 13/08/2014, 05:21

... principal component for the cell lines (PC1, PC2, and PC3), two of the three AD cells lines were close, whereas the third cell line was close in PC2 to the other two cell lines, but separate in PC1 ... close LC cell lines and three SC cell lines were different in PC1, with a negative value for LC and a positive value for SC The 86-2 cell line (LC) was different in PC3 from the SC cell lines Subtypes ... of 19 genes by qPCR The expression of 19 genes in test lung cancer cell lines (black and gray) and four validation cell lines (white) was quantified by qPCR and compared to a normal control cell...

Ngày tải lên: 15/02/2014, 04:20

... comparison of ecdysteroid receptors A B Fig CLUSTALW amino acid alignment of N-terminal (A ⁄ B) domains of LdEcR and DmEcR (A) Alignment of EcRA from the two species (B) Alignment of DmEcRB1, ... (A ⁄ B) domains of EcR are also divergent in the two insect species [31] (Fig 1), although all of the isoforms from both species share almost complete identity over a stretch of 35–37 amino acids ... only from changes in the level of receptor molecule activity, but also from changes in stability and intracellular localization Possible differences in these parameters among EcR–USP dimers from...

Ngày tải lên: 18/02/2014, 08:20

... erythroleukemia cells, Jurkat T-lymphocyte cells, KG1 myeloid cells, H146 small cell lung cancer cells, and HMV-II melanoma cells H146 small cell lung cancer cells were obtained from ATCC (HTB-173) ... internal control (C) Cellular heme contents Cells were cultured for 48 h, and harvested for the measurement of heme content (ng ⁄ 106 cells) mRNA was detected in five of these cell lines, but hardly ... expression levels of HO-1 and HO-2 and cellular heme content The cellular heme content represents the sum of free heme and bound heme of various hemoproteins for maintenance of cellular heme contents...

Ngày tải lên: 19/02/2014, 05:20

... expression in human cell lines We initially analyzed the effects of hypoxia on the expression of HO-1 and HO-2 in human cell lines of Reduced expression of heme oxygenase-2 A B Fig Effects of hypoxia ... expression levels of HO-2 mRNA and protein in five out of six cell lines, with the exception of KG1 myeloid cells A Hypoxia decreases the expression levels of HO-2 protein in YN-1 and K562 cells To confirm ... human cancer cell lines, HeLa cervical cancer and HepG2 hepatoma cells Hypoxia (1% oxygen) decreased the expression levels of HO-1 and HO-2 mRNA after 48 h of incubation in the two cell lines (Fig...

Ngày tải lên: 19/02/2014, 06:20

... of CDO in HCT116 and GEO cell lines on day The activity of CDO in the sensitive cell lines was fitted a standard Emax model Representative DNA histograms following culture with CDO in HCT116 cells ... the reduction in cell number may have been the result of a general and simultaneous blockade of all three phases of the cell cycle Cell proliferation is reduced The reduction in cell number may ... may have been a result of an inhibition of cellular proliferation Therefore, at the end of each of the culture schedules, cells were pulsed with BrdU for 30 mins The extent of BrdU incorporation...

Ngày tải lên: 07/03/2014, 15:20

... capacity of cells The human cancer cell lines chosen included one epidermoid carcinoma cell line, three mammary tumour cell lines and one human pancreas adenocarcinoma All five cell lines exhibited ... 4), as expected for a protein secreted as a result of the cellular production of cystatin C Cellular levels of cystatin C after incubation of the cells in medium containing lm cystatin C in a timescale ... microscope analysis, the tested cell lines showed different patterns of internalization and intracellular distribution of cystatin C In the MCF-7 and A-431 cell lines, CLSM demonstrated that internalized...

Ngày tải lên: 16/03/2014, 06:20

... complex (CRC) (B) UV crosslinking of G-rich GT oligomers Three micrograms of total nuclear extract of CCRF-CEM cells were incubated in buffer C in the presence of ng of the indicated 5¢-end 32P-labelled ... showed that one of these proteins is nucleolin, a multifunctional phosphoprotein whose levels are related to the rate of cell proliferation in a variety of solid tumour cell lines [10] The biological ... haematopoietic cancer cell lines, whereas the G-rich GT oligomer GRO29A was successfully used by Bates to significantly inhibit the growth of a variety of human cancer cells derived from solid tumours...

Ngày tải lên: 16/03/2014, 13:20

... (H2-DCF-DA) was from Molecular Probes Homeostasis of ROS in q0 cells (Eur J Biochem 271) 3647 Cell lines and culture conditions The q+ wild-type osteosarcoma cells (143B) and the q0 cells derived from 143B ... for measurements of intracellular fluorescence Dead cells were excluded by electronically gating data on the basis of forward- vs side-scatter profiles; a minimum of · 104 cells of interest were ... bone q0 cells was DCF oxidation significantly higher compared to the value of the respective q+ cell line (Table 1) Discussion Our analysis of three pairs of q+ and q0 cells, derived from bone,...

Ngày tải lên: 16/03/2014, 18:20

... per cell) , characteristic of leukemic or activated T cells HLA-DR was abundant on all cell lines (‡ · 105 copies per cell) Surface density of class I HLA was low on MT-1 cells (% · 104 per cell) , ... Here, a minimum of 5000 pixels of digital cell images were analyzed in terms of bleaching kinetics and the efficiency of FRET was calculated from the mean bleaching time-constants of the donor dye ... leukemia and lymphoma cell lines (A) Representative FRET efficiency (E, %) histograms measured on T lymphoma/leukemia cell lines, on cell- by -cell basis, using flow cytometry The cell- independent intramolecular...

Ngày tải lên: 17/03/2014, 23:20

... bottleneck in stable transfection of cells arises from the low frequency of stable plasmid integration into genomic DNA At best, only 0.001% of cells generate clones Expansion of the few survivors takes ... propagated in 1% of initially transfected cells [4] EBV plasmid replication has been demonstrated for a large variety of human cell lines; primate and canine cell lines may also be used [5] It has been ... strategy is based on comparative analysis of lysates of cells both with and without transporter expression The expression of all other proteins in the two cell populations should match as closely...

Ngày tải lên: 23/03/2014, 09:21

... gynecological cancer cell lines Methods Cell culture All the cell lines used were described in Table [20-45] and obtained from Cell Lines Service (Eppelheim, Germany) Briefly, all cells were cultured ... in at least one of the cell lines For MMP-8, -12 and -21 only very weak mRNA expression could be observed in single cell lines Nine MMPs, which were present in most of the cell lines, were chosen ... the expression of the other MMPs in endometrial cancer cell lines According to our results, the Ishikawa cell line showed the broadest range of mRNA and protein expression of most of the MMPs analyzed...

Ngày tải lên: 28/03/2014, 14:20

... stabilization of p53 by VRK2 isoforms Fig Expression of two VRK2 isoforms (A) Detection of VRK2 proteins in several cell lines that were determined by immunoblotting of whole -cell extracts Whole extract from ... protein We analysed the localization of VRK2 in two cell lines, WSI, derived from normal skin fibroblasts, and MCF7 cells from a breast carcinoma In both cell lines there was strong staining in ... controlling the cell response to many types of cellular stress [27,28], and its intracellular levels appear to determine the susceptibility of a cell to tumour development [29,30] Regulation of p53 protein...

Ngày tải lên: 30/03/2014, 11:20

... relative proportions of the two enzymes.) pH prole Fig Mathematical modeling of the behavior of a mixture of isoforms of an enzyme (A) Hanes plot of a theoretical mixture of isoforms of an enzyme for ... indicated that the purity of mast cells thus obtained ranged from 65% to 95% of all nucleated cells Isolation of skin mast cells Mast cells were isolated as described previously from infant foreskin ... kinetics of cleavage of a range of chromogenic substrates Materials and methods Isolation of lung mast cells Human lung mast cells were isolated as described previously [39] Briey, cells from macroscopically...

Ngày tải lên: 31/03/2014, 07:20

... undifferentiated cell layer analogous to that observed in rats Establishment of immortalized adrenocortical cell lines A number of cell lines were derived from primary cells prepared from whole adrenal ... induce detectable levels of P450aldo mRNA in the cell lines under current experimental conditions (data not shown) Steroidogenesis of the cell lines The ability of the cell lines to produce steroids ... precursor cell lines for the zona fasciculata cells The available evidence does not support the hypothesis that these cell lines represent bipotential progenitor cell lines or stem cell- like lines...

Ngày tải lên: 31/03/2014, 15:20

... sorafenib in a panel of melanoma cell lines Percentage growth inhibition by A) dasatinib and B) sorafenib in a panel of melanoma cell lines Error bars represent the standard deviation of triplicate ... Sk-Mel-28 melanoma cell lines Effect of4 Effect of dasatinib on (A) invasion and (B) migration in HT144 and Sk-Mel-28 melanoma cell lines Error bars represent the standard deviation of triplicate ... were significantly higher in the three dasatinib sensitive cell lines than in the two resistant cell lines Although the number of cell lines is small, this suggests that EphA2 expression may predict...

Ngày tải lên: 18/06/2014, 15:20

... of 12 0.051; out of 20 0.017; out of 59 0.062; out of 16 0.039; out of 25 0.165; out of Also available online at http://ccr.coriell.org/Sections/Support/Global/QCgenotype.aspx?PgId=412 Page of ... shown Cells from Location C migrated equally to the healthy control whereas cells from Location B have the slowest rate Fibroblasts from the healing edges were found to be most responsive, cells from ... researchers can produce their own cell lines from chronic wounds in a standard fashion These cell lines can provide clinically valuable information on cells derived from chronic ulcers Competing...

Ngày tải lên: 18/06/2014, 15:20