tài liệu hóa sinh y học

... vật thổ nhưỡng, vi sinh vật thú y, vi sinh vật thực vật, vi sinh vật công nghiệp và vi sinh vật y học Vi sinh vật y học (Medical Microbiology) chuyên nghiên cứu về các vi sinh vật ảnh hưởng tới ... chắnh xác, khoa học; cập nhật các tiến bộ khoa học, kỹ thuật hiện ựại và thực tiễn Việt Nam Sách VI SINH Y HỌC ựã ựược Hội ựồng chuyên môn thẩm ựịnh sách và tài liệu dạy Ờ học chuyên ngành Cử nhân ... CỦA VI SINH Y HỌC 1 ðỐI TƯỢNG NGHIÊN CỨU Vi sinh vật học (Microbiology) là môn học nghiên cứu về những sinh vật nhỏ bé, mắt thường không nhìn thấy ñược; bao gồm nhiều phân môn như: vi sinh vật

Ngày tải lên: 26/10/2014, 20:33

... nhầy,có máu Cấy phân Cách ly, khửtrùng vệ sinh Kháng sinh đồ enzym Đường Gra m Di độn g bào Khí Môi trường Khuẩn lạc Ghi chú Cat a las e Ox y das e Trang 4Salmonella - + - - TùyS.Typhi S.parytyphy ... nguyên Độc tố, enzym Đường Gra m Di độn g bào Khí Môi trường Khuẩn lạc Ghi chú Cat a las e Ox y das e liệu Vàng da xuất huyết Chưa có tài liệu thể Nuô i cấy Sin h vật Kháng nguyên Độc tố, enzym ... loại, thơm, mt xanh sắc tố: 1: Pyocyanin(c hỉ có trực khuẩn mủ xanh) 2: Pyoverdin 3: Pyorubrin 4: Pyomelanin O gây bệnh do Ngoại độc tố A gây bệnh có điều kiện gây bệnh ở đường tiểu và vết thương

Ngày tải lên: 15/09/2015, 18:29

... 1HÓA SINH HỌCCHUYỂN HÓA CÁC CHẤT, OXY HÓA SINH HỌC CHU TRÌNH ACID CITRIC (KREBS) BM Hóa sinh TS Trần Thanh Nhãn Trang 2MỤC TIÊU1 Trình bày được những khái niệm về chuyển hóa các chất và chuyển ... chuyển hóa trong cơ thể - Phục vụ cho công tác phòng và chữa bệnh Trang 192 OXY HÓA SINH HỌC2.1- Phản ứng oxy hóa-khử Định nghĩa : - Là phản ứng trong đó có sự cho và nhận điện tử hay có sự thay ... Rotenon và Amytal ức chế sự vận chuyển điện tử từ NADH dehydrogenase và như vậy ức chế sự oxy hóa NADH (complex I) Tuy nhiên, sự oxy hóa FADH2 vẫn cóthể thực hiện bởi vì điện tử vẫn được chuyển từ

Ngày tải lên: 22/02/2014, 21:20

... gầy đi, đi ngoài Khi bộc phát bệnh viêm dạ dày mãn tính, yếu tố “ẩm thực” chiếm một vị trí rất quan trọng Vì thế hình thành một thói quen ăn uống tốt là diệu pháp để trị liệu bệnh này Đây là ... có quy luật, kỵ ăn nhanh uống nhanh và không đúng giờ giấc 3 Chú ý vệ sinh thực phẩm, kiên quyết không để cho vi sinh vật ở thế giới bên ngoài xâm nhập và làm nguy hại đến niêm mạc dạ dày 4 Dung ... nguy hiểm khi cơ thể nổi mề đay cấp tính Lúc này, người bệnh sẽ bị khó thở, tụt huyết áp nhanh và đột ngột, dị ứng trên khắp cơ thể Khi bị nổi mề đay cấp tính cần được nhanh chóng cấp cứu ngay

Ngày tải lên: 26/01/2014, 04:20

... rapidly inactivated by the 6-O-bromoacetyl esters of methyl a-D-glucopyranoside (1a) and methyl a-D -manno-pyranoside (1c), methyl 6-deoxy-6-isothiocyanato-a-D -glucopyranoside (1e), b-D-glucopyranosyl ... 40%) Methyl 2,3-anhydro-a-D -allopyr-anoside (2a) and methyl 3,4-anhydro-a-D -galactopyrano-side (2b) were obtained by desilylation of 1 mmol of the 6-O-[dimethyl-(1,1,2-trimethylpropyl)silyl]-protected ... methyl 6-deoxy-6-isothiocyanato-a-D-glucopyranoside (1e) [24], and b-D -glucopyranosyl isothiocyanate (3c) [25] were prepared by the reported procedures Methyl 6-O-chloroacetyl-a-D -glucopyranoside

Ngày tải lên: 21/02/2014, 01:21

... New York, USA Oligonucleotide synthesis Oligonucleotides were synthesized on an Applied Biosys-tems model 392 DNA synthesizer and purified by prepar-ative gel electrophoresis in 20% polyacrylamide ... above Assay for superoxide dismutase activity The specific activity of SOD was measured spectrophoto-metrically by its inhibitory action on the superoxide-dependent reduction of cytochrome c by xanthine-xanthine ... with hydrogen peroxide and catalase to ensure there were no adverse effects on the SOD assay Polyacrylamide gel electrophoresis (PAGE) Native 8% polyacrylamide gels (acrylamide : N,N¢-methyl-enebisacrylamide,

Ngày tải lên: 21/02/2014, 01:21

... are currently investigating this possibility Alternatively, this enzyme may be involved in the epimerization reaction necessary for the biosynthesis of the capsular heptopyranose as alditol ... Absolute configuration was assigned by characterization of the but-2-yl glycosides in GLC-MS [21]. Methylation analysis was carried out by the NaOH/ dimethylsulfoxide/CH 3 I procedure [22] using ... deduced by the identification of but-2-yl chiral glycosides in GLC- MS [21]. Sugar linkage analysis (Table 1), by the methyla- tion procedure [22], on the LOS revealed the following sugar linkage types:

Ngày tải lên: 21/02/2014, 01:21

... Proline, hydroxyproline and hydroxylysine were examined at 254 nm by reverse-phase HPLC of their phenylisothiocarbamate derivatives [26], as reported previously [20] The serine, threonine and tyrosine ... acid-rich in WSM Glycosaminoglycan analysis Glycosaminoglycans are highly negatively charged because of the presence of sulfate ester and/or carboxyl groups Therefore, they interact with cations ... (WIM) Again we found a high content in Gly-Ala, and the global composition was very similar to the WSM, and consequently the EDTA-IM Hydroxyproline, hydroxy-lysine and phosphoserine were not detected

Ngày tải lên: 21/02/2014, 01:21

... efficiently oxidized by the enzymes of the respiratory chain, specifically designed for the NADH oxidation However, the current study shows that superoxide is cata-lytically produced by the complex-bound ... Because CO2and the 1-hydroxy-3-carboxypropyl moiety bound to ThDP are formed during the E1-catalyzed decarboxylation of 2-oxoglutarate (reac-tion 1), the spectrum and hyperfine splitting constants ... acyltransferase; E3, dihydrolipoamide dehydrogenase; DTPA, diethylene-triaminepentaacetic acid; DMPO, 5,5¢-dimethyl-1-pyrroline-N-oxide; MNP, 2-methyl-2-nitrosopropane; PBN, a-phenyl-N-tert-butylnitrone;

Ngày tải lên: 21/02/2014, 01:21

... Holliday junctions The stoichiometry analysis presented here (see Table 1), clearly shows that two RuvA tetramers bind to synthetic Holliday junctions, which is in agreement with the crystal structure ... Molecular Biology and Biotechnology, University of Sheffield, UK; 4 Krebs Institute, Centre for Chemical Biology, University of Sheffield, UK The RuvABC resolvasome of Escherichia coli catalyses the resolution ... Holliday junction. MATERIALS AND METHODS Synthesis and purification of oligodeoxynucleotides used in the binding studies Oligonucleotide synthesis was performed on an Applied Biosystems 394 DNA synthesiser

Ngày tải lên: 21/02/2014, 01:21

... the cystatin F produced by U937 cells is retained intracellularly or is taken up by the cells immedi- ately following secretion. Accordingly, the relatively low amount of cystatin F produced by ... correlate exactly with the mobilities of di-, mono- and unglycosylated forms of recombinant cystatin F previously characterized by enzymatic removal of N-linked carbohydrate and carbohydrate composition analysis ... di-glycosylated (band A) species at approx. equal proportions [5], it was evident that native human cystatin F exists in these two glycosylated forms, but also as one most likely unglycosylated

Ngày tải lên: 21/02/2014, 01:21

... VPR additionally contains Cys277 and Cys281, and Cys351 and Cys362 in the C-terminal domain, that supposedly also form disulfide bonds as the enzyme has not been found to contain any free thiol ... related enzymes, especially to the mesophilic enzymes from V alginolyticus and V cholerae (Table 1) These enzymes all contain relatively high content of Ser and Thr, as well as Gly, probably reflecting ... nm, respectively Cuvettes were thermostatted at 25 °C The denaturation curves were normalized according to Fu ¼ (yƒ ) y)/ (yƒ ) yu), assuming a two-state transition, where yƒ and yu are the extrapolated

Ngày tải lên: 21/02/2014, 01:21

... in the primary hereditary cardiomyopathy of the Syrian hamster I Regulation of prodynorphin gene expression by nuclear protein kinase C J Biol Chem 272, 6685–6692. 22 Ishizuka, S., Yano, T., Hagiwara, ... activation may have not been entirely encompassed by the drug-treatment Such a hypo-thesis is further confirmed by the observation that, differ-ently from the effect of serum, the stimulatory effect ... phosphorylation may be lying upstream of ERK1/2 Recent observations indicate that activation of Raf by PMA may trigger the same signaling pathway as oncogenic Raf, or Raf activation by Ras in

Ngày tải lên: 21/02/2014, 01:21

... of their cytotoxic activity, as measured by LDH release, was obtained by DM64 at an equimolar ratio (Fig 6) DM64 alone was devoid of myotoxicity and cytotoxicity in these experimental systems DM64 ... 7catalytically active PLA2, the membrane disorganizationseems to be potentiated by enzymatic degradation of phospholipids [7,44] The capacity of DM64 to inhibit the myotoxicity induced by myotoxins ... speculate that DM64 binds to the myotoxins through a myotoxic/ cytolytic site distinct from the catalytic site, as already described for the inhibition of myotoxicity by heparin [44] At least in the

Ngày tải lên: 21/02/2014, 01:21

... purification A thalianaseeds, ecotype Columbia were kindly provided by F Floto, and cell suspension culture by O Mattsson, both at the Department of Plant Physiology, University of Copenhagen Plants were ... resolution by X-ray crystallography [33] Moreover, HRPC has been solved at 1.8 A˚ resolution in complex with the substrate analog benzhydroxamic acid [34], and at 1.45 A˚ resolution in the ternary complex ... peroxidases presently known Figure 1 shows the predicted side chain ligands in blue, and demonstrates that they are very well conserved Main chain carbonyl oxygen and a water molecule hydrogen-bonded

Ngày tải lên: 21/02/2014, 01:21

... thermodynamic analysis by laser-flash absorption spectroscopy of photosystem I reduction by plastocyanin and cytochrome c 6 in Anabaena PCC 7119, Synechocystis PCC 6803, and spinach. Biochemistry 35, ... possibility is that primitive cyano- bacteria at the time of chloroplast origin had poorly defined interactions between Cyt f and plastocyanin and/or plasto- cyanin and photosystem I, and subsequently ... (NaCl) of k 2 for wild-type P. laminosum plastocyanin reacting with wild-type P. laminosum photosystem I and wild-type spinach plastocyanin reacting with wild-type spinach photosystem I. Experimental

Ngày tải lên: 21/02/2014, 01:21

... composed of dimyristoylglycerophosphocholine (Myr 2 Gro-PCho), dimy- ristoylglycerophosphoethanolamine (Myr 2 Gro-PEtn) and dimyristoylglycerophosphoglycerol (Myr 2 Gro-PGro) by DSC, in order ... Myr 2 Gro-PCho, dimyristoylglycerophosphocholine; Myr 2 Gro-PEtn, dimyristoylglycerophosphoethanolamine; Myr 2 Gro-PGro, dimyristoylglycerophosphoglycerol; DSC, differential scanning calorimetry; L a , ... 1-palmitoyl-2-oleoyl-glycerophosphocholine; PamOleGro-PEtn, 1-palmitoyl- 2-oleoyl-glycerophospholamine; PamOleGro-PGro, 1-palmitoyl-2-oleoyl-glycerophosphoglycerol. *Present address: Department of Chemistry,

Ngày tải lên: 21/02/2014, 01:21

... 0.5 mMphenylmethanesulfonyl¯uoride], and the samples were fractionated by electrophoresis on a 12% SDS/polyacrylamide gel The separated polypeptides were electotransferred to a poly(vinylydene di¯uoride) ... O9W4W6) and indicated by the arrowheads The symbol ÔVÕ indicates a potential N-glycosylation site A putative polyadenylation signal is enclosed by a box (B) Hydrophobicity plot of DmNST/DmUGT ... N-glycosylation site in the DmUGT during analysis on the primary structure of the fruit ¯y NST (Fig 1A) Based on the 10-segment trans-membrane model proposed by Eckhardt et al [27], the N-glycosylation

Ngày tải lên: 21/02/2014, 15:20

... Arg40-Phe82(Cys66-Cys160)Lys150-Ile162 Val43-Phe82(Cys66-Cys160)His146-Ile162 Leu58-Phe82(Cys66-Cys160)Lys150-Ile162 Glu62-Phe82(Cys66-Cys160)Lys150-Ile162 Lys60-Phe82(Cys66-Cys160)Lys150-Ile162 Val41-Lys70(Cys66-Cys160)Leu149-Ile162 ... Val41-Lys70(Cys66-Cys160)Leu149-Ile162 Val41-Lys69(Cys66-Cys160)Leu149-Ile162 Leu58-Lys70(Cys66-Cys160)Leu149-Ile162 Trp61-Lys69(Cys66-Cys160)Leu149-Ile162 Trp61-Lys70(Cys66-Cys160)Leu149-Ile162 Leu58-Lys69(Cys66-Cys160)Leu149-Ile162 ... enzymes are available at very high purity; (c) both enzymes are highly specific; and (d) they act on complementary sets of amino acids (hydrophobic, chymotrypsin; basic, trypsin) Other enzymes

Ngày tải lên: 21/02/2014, 15:20

... identified by thin-layer chromatography, immunologically and by high-performance liquid chromatography The biological activity of (2–5)A oligomers was verified by inhibition of the protein synthesis ... quantify the synthesis products Dephosphorylation of (2–5)A oligomers (2–5)A synthetase assay (2–5)A synthetase activity in crude extracts was determined after binding of the enzyme to a positively ... reticulocyte lysate [30] The (2–5)A synthetase reaction products were also confirmed by MALDI-MS and by NMR analysis [31] We succeeded in cloning the sponge (2–5)A synthetase from G cydonium [32]

Ngày tải lên: 21/02/2014, 15:20