studies in mechanobiology tissue engineering and biomaterials volume 2

... Summary of key findings 109 6.2 Future work 110 6.2.1 Assessing functional vascularization 110 6.2.2 Applying our findings pertaining to PDCA-Gelfoam 112 6.2.3 Developing PHI-delivering materials ... regulation of HIF-1 21 2.2.3 PHIs stimulate angiogenesis 24 2.3 Potential applications of PHIs 25 2.3.1 Ischemic and fibrotic diseases 25 2.3.2 Wound and fracture healing 28 2.4 Potential applications ... of PHIs in tissue engineering 31 Chapter 3 Incorporation of a PHI into Scaffolds: A Vascularization Strategy for Tissue Engineering Applications 33 3.1 Introduction 34 3.2 Hypothesis and objectives...

Ngày tải lên: 09/09/2015, 11:11

... surfaces. Small, 2005. 1(1): p. 83-6. 152 212. 213. 214. 215. 216. 217. 218. 219. 220. 221. 222. 223. 224. 225. 226. 227. 228. 229. Cai, S., et al., Injectable glycosaminoglycan hydrogels ... fibers. Biomaterials, 2008. 29(15): p. 2348-58. 153 230. 231. 232. 233. 234. 235. 236. 237. Cao, H., et al., The topographical effect of electrospun nanofibrous scaffolds on the in vivo and in ... 2007. 1(2): p. 110-9. 142 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. Thevenot, P., W. Hu, and L. Tang, Surface chemistry influences implant biocompatibility....

Ngày tải lên: 10/09/2015, 15:52

... standards and for the curricular material on which they might ultimately be based [For more information, see: National Academy of Engineering (2009) Engineering in K-12 Education: Understanding ... Science Board (2008) Science and Engineering Indicators: 2008 Arlington, VA: National Science Foundation. 36 GAO (2006) Science, Technology, Engineering, and Mathematics Trends and the Role of ... shrank from 32 to 26 scale points between 1990 and 2007, and the average gap decreased from 2000 to 2007 between black and white eighth graders after increasing between 1990 and 2000. 16 National...

Ngày tải lên: 12/02/2014, 19:20

... walled in and walled off by segregation These include the priorities of uplift, of establishing distinctly black institutions and entertainments, and of celebrating and projecting racial ... other black businessmen in incorporating the team in 1907 They included in the new corporation a summer resort, roller skating rink, and restaurant, with a goal of establishing “a black ... [...]... Sarah, and Mary Arden—for joining in the journey and sharing in its swamps, valleys, jungle paths, and mountain views We did this together in true partnership I also...

Ngày tải lên: 23/03/2014, 22:20

... Smitten and coworkers [122] determined the SIR of lymphoma to be 2.08 (95% CI = 1.80 to 2.39) in RA Recent research has linked smoking exposure to increased incidence of developing RA [124,125] After ... be 0.26% in 1975, and a multicenter study [80] reported the prevalence to be 0.95% in 1995 Various studies revealed that both gout and hyper-uricemia have been increasing in the USA, Finland, ... diseases and highlight the key role of epidemiological research in understanding these intriguing conditions Introduction Epidemiology has taken an important role in improving our understanding of...

Ngày tải lên: 09/08/2014, 14:20

... differentiation………………… 21 2.3.4.1 Cartilage tissue engineering using hydrogels……………………… 21 2.3.4.2 Cartilage tissue engineering using polymeric scaffolds…………… 23 2.4 Cartilage formation and regeneration using ESCs………………………… ... Immunofluorescence (IF) staining………………………………… 53 3.7.2 Staining of tissue specimens……………………………………… 54 3.7.2.1 Processing of tissue specimens…………………………………… 54 3.7.2.2 Haematoxylin and eosin staining………………………………… ... chondrogenesis Tissue Eng Part A 15(2):243-254 Chung C, Burdick JA (2008) Engineering cartilage tissue Adv Drug Deliv Rev 60(2):243-262 Review Clair BL, Johnson AR, Howard T (2009) Cartilage...

Ngày tải lên: 11/09/2015, 09:57

... (m, 22 H), 0. 92 (t, 3H); 13C NMR (300 MHz, C6D6) δ 154.8, 94.6, 85.6, 80 .2, 79.7, 79 .2, 65.0, 64 .2, 63.8, 63 .2, 62. 8, 61.9, 32. 0, 29 .8, 29 .6, 29 .4, 29 .2, 28 .9, 28 .7, 28 .0, 25 .8, 25 .4, 23 .2, 22 .7, ... SPHK Inhibitors 3. 72 (m, 2H), 2. 18 (t, 2H), 1.43 (s, 9H), 1 .24 (m, 12H), 0.85 (t, 3H); 13C NMR (300 MHz, CDCl3) δ 156 .2, 87.9, 80.0, 64.3, 62. 6, 55.8, 31.7, 29 .1, 29 .0, 28 .8, 28 .4, 28 .2, 22 .5, ... 64.44% 72. 98% 63.88% Structures of six obtained compounds (analogues of sphingosine) 2. 2 .2 Compounds Inhibitory Function on SPHK Activity in vitro 2. 2 .2. 1 Over-expression of SPHK1 and SPHK2 2. 2 .2. 1.1...

Ngày tải lên: 11/09/2015, 16:06

... 28 days vs OD 28 days, P

Ngày tải lên: 11/09/2015, 16:06

... were incubated on ice for 20 min, followed by two washes in 2% FCS/PBS, before resuspension in 100 μl of 4% paraformaldehyde and incubation on ice for 20 Cells were again washed twice in 2% FCS/PBS ... cultured in 6-well plates were washed twice with 1x PBS and starved in serum-free DMEM for half an hour 1μM S1P was then added into the wells and incubated at 37°C for 5min, 10min, 30min, and 60min ... 5μM Fura2/AM (Molecular Probes, Invitrogen Singapore) Human BM- and AD- MSCs were separated into samples of 0 .2 million cells, and were incubated at 37oC for 30 and agitated every 15 minutes Afterwards,...

Ngày tải lên: 11/09/2015, 16:06

... 10(19): 28 18 -22 Shu, X., W Wu, et al (20 02) "Sphingosine kinase mediates vascular endothelial growth factor-induced activation of ras and mitogen-activated protein kinases." Mol Cell Biol 22 (22 ): ... Sacket, et al (20 08) “Characterization of N, N-dimethyl-derythro-sphingosine-induced apoptosis and signaling in U937 cells: independence of sphingosine kinase inhibition.” Prostaglandins & Other ... N,N-dimethyl-Derythro-sphingosine-induced apoptosis and signaling in U937 cells: Independece of sphingosine kinase inhibition.” Prostaglandins & other lipid Mediators 86: 18 -25 Kim, Hyo-Lim, Santosh...

Ngày tải lên: 11/09/2015, 16:06

... FTY 720 P, is an agonist of all S1P receptors except S1PR2 (Brinkmann et al., 20 02; Mandala et al., 20 02; Taha et al., 20 06) By preventing S1P binding to S1PRs, FTY- 720 P induced an increase in lymphocyte ... al., 20 02; Pitson et al., 20 02) These domains may play a role in substrate recognition (Pitson et al., 20 02) Both SPHK1 and SPHK2 are capable of phosphorylating erythro-sphingosine, dihydrosphingosine ... could exert a proinflammatory effects by promoting neutrophil chemotaxis (Cummings et al., 20 02; Ibrahim et al., 20 04), and induced certain proteins Chapter Introduction important in inflammation,...

Ngày tải lên: 11/09/2015, 16:06

... direct binding to canonical binding sites in the rDNA promoter (Arabi et al 20 05; Grandori et al 20 05; Poortinga et al 20 04) rDNA genes are transcribed by polymerase I in the nucleolus, arguing that ... since Myc leads to an activation of E2F-dependent transcription and distinct activating E2F proteins act downstream of Myc in controlling cell proliferation and tumorigenesis (Baudino et al 20 03; ... Smith C, Nevins JR (20 01) Myc requires distinct E2F activities to induce S phase and apoptosis Mol Cell 8:105–113 Lindstrom MS, Wiman KG (20 03) Myc and E2F1 induce p53 through p14ARF -independent...

Ngày tải lên: 25/10/2013, 21:20

... obtained data indicating that a-defensin-1 and a-defensins -2 induce an increase in the protein content of cyclin D and that quercetin inhibits the a-defensininduced increase in cyclin D protein ... caused by a-defensin-1 and a-defensin -2 p38 mitogen-activated protein (MAP) kinase inhibitor SB203580 and phosphoinositide 3-kinase (PI3K) inhibitor LY2940 02 prevented increases in the phosphorylation ... a-defensin-1 and a-defensin -2 (2. 5 lm) in the presence and absence of quercetin (10 lm) was determined As shown in Fig 4, incubation of lung fibroblasts with a-defensin-1 and a-defensin -2 resulted in...

Ngày tải lên: 18/02/2014, 06:20

... laminin chains in bovine follicles J Reprod Fertil 1998, 1 12( 2) :26 7 -27 8 Zhao Y, Luck MR: Gene expression and protein distribution of collagen, fibronectin and laminin in bovine follicles and ... protein kinase in insulin and insulin-like growth factor I (IGF-I) signaling cascades for progesterone and IGF-binding protein-1 production in human granulosa cells J Clin Endocrinol Metab 20 03, ... alpha(4)/alpha(9) integrins, alpha(6) integrins, and CD9 in the interaction of the fertilin beta (ADAM2) disintegrin domain with the mouse egg membrane Biol Reprod 20 02, 66(4):1193- 120 2 Chen CC, Chen N, Lau...

Ngày tải lên: 05/03/2014, 17:20

... cervical cancer 14 15 16 17 18 19 20 21 22 23 24 25 benz(a)anthracene-induced skin papillomas and carcinomas by dehydroepiandrosterone and 3-b-methylandrost-5-en-17-one in mice Cancer Res 45, 164–166 ... Candas B (1998) DHEA and the intracrine formation of androgens and estrogens in peripheral target tissues: its role during aging Steroids 63, 322 – 328 Lu SF, Mo Q, Hu S, Garippa C & Simon NG (20 03) ... Counts 20 30 G1 CD 20 0 400 600 FL2-A 800 1000 20 0 400 600 FL2-A 800 1000 400 600 FL2-A 800 1000 20 0 400 600 FL2-A 800 1000 20 0 400 600 FL2-A 800 1000 Counts 20 10 0 40 40 30 30 Counts 20 Counts 20 ...

Ngày tải lên: 16/03/2014, 00:20

... protein kinase A, extracellular signal-regulated kinase ⁄ 2, and phosphatidyl inositol 3-kinase ⁄ Akt signaling Endocrinology 148, 5 12 529 Cui QL, Fogle E & Almazan G (20 06) Muscarinic acetylcholine ... 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Ba Curr Biol 7, 26 1 26 9 Fayard E, Tintignac LA, Baudry A & Hemmings BA (20 05) Protein kinase B ⁄ ... preventing PI3K activation [36] Following agonist-induced activation, dopamine D2 receptors are internalized and form a multiprotein complex that includes b-arrestin, protein phosphatase 2A and...

Ngày tải lên: 16/03/2014, 05:20

... the number of bands and the labeling intensity were increased in growing cells The most prominent increases were in bands of 140, 133, 116, 95, 82, 73 and 56 kDa (Fig 4) Other bands also showed ... Characterization of botulinum C3-catalyzed ADP-ribosylation of rho proteins and identification of 20 21 22 23 24 25 26 27 28 29 30 31 32 mammalian C3-like ADP-ribosyltransferase Mol Cell Biochem 138, ... FEBS 20 03 1 02 L Yau et al (Eur J Biochem 27 0) include various GTP-binding proteins, including Gs, Gi and Rho [13–15] Although these target proteins suggest a role for mono-ADP-ribosylation in signal...

Ngày tải lên: 17/03/2014, 09:20

... peptidases, DP8 and DP9 15 16 17 18 19 20 21 22 23 24 25 26 27 invasive activity attributable to dipeptidyl peptidase IV overexpression in ovarian carcinoma Cancer Res 62, 27 53 27 57 Wesley UV, Albino AP, ... b1-integrin [29 ], so the effects of DP8 and DP9 on p38, b1-integrin and DDR1 phosphorylation require examination Changes in TIMP2 and b-catenin expression may be secondary to effects on integrins and ... fibronectin and integrin binding [10,18 ,29 ] The increased STS-induced apoptotic effect of DP8 and DP9 may indicate that under certain biological FEBS Journal 27 3 (20 06) 24 47 24 60 ª 20 06 The Authors...

Ngày tải lên: 23/03/2014, 11:20

... (PAK2) [28 ], MAPK kinase kinase (MEKK-1) [29 ], MAPK kinase kinase kinase (Raf-1), protein kinase B (Akt) [30,31] and protein kinase C (PKC) [ 32] PKC is a family of serine ⁄ threonine protein kinases ... adhesion complexes consist of integrin proteins, the tyrosine kinases Src and focal adhesion kinase (FAK), actin-binding structural proteins, and the adaptor proteins paxillin and Crk-associated substrate ... tricarbonyl J Neurosci 22 , 1738–1751 Kaasinen SK, Golksteins G, Alhonen L, Janne J & ¨ Koistinaho J (20 02) Induction and activation of protein kinase Cd in hippocampus and cortex after kainic acid treatment...

Ngày tải lên: 23/03/2014, 11:20

... Following intron (kb) Exon PP17b ADFP PLIN PP17b ADFP PLIN ADFP PLIN PP17b ADFP PLIN 111 82 198 84 28 5 20 1 126 943 – 54 52 195 84 28 5 183 135 879 – 1 02 58 20 4 84 26 4 174 1 92 246 1577 – 22 66 28 ... 1486. 726 5 1707.9407 1834. 929 9 20 59.0460 20 66.9783 20 73. 126 6 20 76.0 726 20 89. 121 5 125 117 93 71 62 62 62 44 47 )44 71 52 49 47 61 59 pyroGlu 48–55 48–55 65–74 1– 12 1 32 1 42 63–74 65–76 114– 124 114– 124 ... 28 95 67 42 114 – – 10 65 28 95 61 45 133 – – 15 68 28 88 58 64 82 129 – 18 /27 56/ 82 54/75 43/70 36/70 26 /50 40/70 – – 21 / 42 38/68 54/79 20 / 42 13 /29 14/40 15/38 – 6 .2 1.3 0.1 7.3 4.1 2. 9 5.1 –...

Ngày tải lên: 23/03/2014, 21:20