structure function and dynamics with mass spectrometry

... properties, and function We can determine the struc-ture of short DNA fragments with picometer precision, find majority ofthe genes encoded in DNA, and we can manipulate, stretch and twist in-dividual ... catenation, and the definitions of linking number and supercoiling Itcontinues with an outline of continuum and discrete models of DNA elas-ticity, focusing on local energy contributions and analysis ... for Math- ematics and its Applications (IMA), Alfred P SLoan Fellowship and NSF Grant DMS 0516646. Pitts-293 C.J Benham et al (eds.), Mathematics of DNA Structure, Function and Interactions,

Ngày tải lên: 11/06/2017, 20:28

... centrifuge tubes) were washed with soap and water and rinsed with acetone and n-hexane prior to use and between samples Samples: Blank sample was lean pork meat and grilled pork was purchased ... meat and meat products by gas chromatography tandem mass spectrometry Tạp chí phân tích Hóa, Lý và Sinh học, 21(4) Tomasz Rejczak, Tomasz Tuzimski (2015) A review of recent developments and trends ... compounds (BaP) have adverse and toxic effects on the cells, tissues, development and immune system of animals (Essumang et al., 2013; Manda et al., 2012) Figure 1 Chemical structures for 16 PAHs used

Ngày tải lên: 20/12/2019, 16:39

... centrifuge tubes) were washed with soap and water and rinsed with acetone and n-hexane prior to use and between samples Samples: Blank sample was lean pork meat and grilled pork was purchased ... meat and meat products by gas chromatography tandem mass spectrometry Tạp chí phân tích Hóa, Lý và Sinh học, 21(4) Tomasz Rejczak, Tomasz Tuzimski (2015) A review of recent developments and trends ... compounds (BaP) have adverse and toxic effects on the cells, tissues, development and immune system of animals (Essumang et al., 2013; Manda et al., 2012) Figure 1 Chemical structures for 16 PAHs used

Ngày tải lên: 14/01/2020, 17:37

... pollutants and identified as carcinogenic, mutagenic and teratogenic in humans The goal of this paper was to develop a process to analyze the content of 16 common PAHs in grilled pork by mass spectrometry ... Rugged and Safe) extraction and GC-MS in SIM mode analysis allowed for quantification of 16 PAHs in very small concentrations (10 - 100ppb) with recoveries of 83.8 to 113.4% on spiked sample and ... OF POLYCYCLIC AROMATIC HYDROCARBONS IN BARBECUED PORK USING QUECHERS EXTRACTION AND GAS CHROMATOGRAPHY – MASS SPECTROMETRY Nguyen Van Phuc1 Ho Chi Minh City University of Food Industry ARTICLE

Ngày tải lên: 09/02/2020, 09:07

... centrifuge tubes) were washed with soap and water and rinsed with acetone and n-hexane prior to use and between samples Samples: Blank sample was lean pork meat and grilled pork was purchased ... and giving a warning for human health MATERIAL AND METHODS Reagents and materials Reagents: A mixture of 16 PAHs (2000 mg.L-1 in hexane and a purity of> 99%) was supplied by Merck (Germany) and ... (2012) and Tran Cao Son et al., (2016) showed that most samples of food processed by roasting, frying and baking in Vietnam were contaminated with PAHs, including BaP Food samples were found with

Ngày tải lên: 02/03/2020, 16:02

... centrifuge tubes) were washed with soap and water and rinsed with acetone and n-hexane prior to use and between samples Samples: Blank sample was lean pork meat and grilled pork was purchased ... meat and meat products by gas chromatography tandem mass spectrometry Tạp chí phân tích Hóa, Lý và Sinh học, 21(4) Tomasz Rejczak, Tomasz Tuzimski (2015) A review of recent developments and trends ... compounds (BaP) have adverse and toxic effects on the cells, tissues, development and immune system of animals (Essumang et al., 2013; Manda et al., 2012) Figure 1 Chemical structures for 16 PAHs used

Ngày tải lên: 01/05/2021, 11:19

... Access16S-ARDRA and MALDI-TOF mass spectrometry as tools for identification of Lactobacillus bacteria isolated from poultry Marta Dec* , Andrzej Puchalski, Renata Urban-Chmiel and Andrzej Wernicki ... Poland A total of 89 bacterial strains, including 46 strains from geese, 35 from broilers and 9 from turkeys, were isolated on MRS (Man, Rogosa and Sharp) medium (BTL, Poland) supplemented with ... with 1μL 70 % formic acid and then with 1μL matrix solution containing 10 mg/mL HCCA (a-cyano-4-hydroxycinnamic acid, Sigma-Aldrich, Poland) resolved in 50 % acetonitrile (Sigma-Aldrich, Poland)

Ngày tải lên: 19/11/2022, 11:35

... practices of flavours and fragrances by mass spectrometry Frédéric Begnaud and Alain Chaintreau Firmenich SA, Corporate R&D Division, Route des Jeunes 1, CH-1211 Geneva 8, Switzerland AC,0000-0002-1665-0521 ... article is part of the themed issue ‘Quantitative mass spectrometry’ 1 Introduction Gas chromatography-mass spectrometry (GC-MS) has been the gold standard for the identification of natural ingredients ... t-test. (ii) Purity assessment of internal and calibration standards The easiest way to obtain pure standards is to purchase them with certified identity and purity However, chemicals can deteriorate

Ngày tải lên: 04/12/2022, 10:36

... in HIPV production [35], with GLVs associ-ated with both mechanical damage and herbivory, and monoterpenoids, sesquiterpenoids, and homoterpenes specifically associated with herbivory In particular, ... was used as internal standard (I.S.) and was sorbed onto the tapes as proposed by Wang et al [70] for Solid Phase Micro Extraction Preliminary analysis with tapes with and without camphor I.S were ... distribution between GLVs and the terpenoid groups The position of (k) and (o) and the absence of (t) and (u) suggest the non-significant role of terpenoids in MD reaction, unlike the HW and MDOS loading

Ngày tải lên: 26/05/2020, 20:53

... R C H A R T I C L E Open AccessMolecular dynamics and structure function analysis show that substrate binding and specificity are major forces in the functional diversification of Eqolisins Nicolás ... diversification related to substrate specificity and substrate binding Keywords: Structure-function prediction, Functional redundancy and diversification, Molecular dynamics, Acid protease, Eqolisin, Glutamic ... the WT under Andersen thermostat/barostat were used as reference for cross-correlations analysis, lowest normal modes visualization, and salt- and H-bridges inspection and comparison with the results

Ngày tải lên: 25/11/2020, 14:24

... Valkenborg, D, Loo, JA, and Sobott, F (2018) Radical solutions: Principles and application of electron-based dissociation in mass spectrometry-based analysis of protein structure Mass Spectrometry Reviews,' ... author) (2018) Radical solutions: Principles and application of electron based dissociation in mass spectrometry based ‐ ‐ analysis of protein structure Mass Spectrometry Reviews, 37 (6) pp 750-771 ... the record and the reason for the withdrawal request Trang 2Radical solutions: Principles and application of electron-based dissociation in mass based analysis of protein structure spectrometry-Frederik

Ngày tải lên: 28/10/2022, 00:21

... Valkenborg, D, Loo, JA, and Sobott, F (2018) Radical solutions: Principles and application of electron-based dissociation in mass spectrometry-based analysis of protein structure Mass Spectrometry Reviews,' ... author) (2018) Radical solutions: Principles and application of electron based dissociation in mass spectrometry based ‐ ‐ analysis of protein structure Mass Spectrometry Reviews, 37 (6) pp 750-771 ... the record and the reason for the withdrawal request Trang 2Radical solutions: Principles and application of electron-based dissociation in mass based analysis of protein structure spectrometry-Frederik

Ngày tải lên: 28/10/2022, 00:22

... GPR17: Molecular modeling and dynamics studies of the 3-D structure and purinergic ligand binding features in comparison with P2Y receptors Address: 1 Laboratory of Cellular and Molecular Pharmacology ... responding to ADP and ATP This group includes human and rodent P2Y1, P2Y12, and P2Y13, and human P2Y11; (2) uracil nucleotide-preferring receptors This group includes human P2Y4 and P2Y6 responding ... selectivity (human and rodent P2Y2, rodent P2Y4 and, possibly, P2Y11); and (4) receptors responding solely to the sugar nucleotides UDP-glucose and UDP-galactose (P2Y14) [1] From a phylogenetic and structural

Ngày tải lên: 02/11/2022, 10:45

... proteins with less uniform patterns across the eight patients Proteins identified by mass spectrometry were classified by subcellular location and molecular function (Fig 3) To better understand the ... patients confidence and quantitation accuracy for proteomic research, especially for low-abundance proteins [16,17] We used iTRAQ labeling together with gel-assisted digestion and mass spectrometry ... patient were labeled with iTRAQ115 The resulting peptides from normal tissue of another patient were labeled with iTRAQ116 and peptides from tumor tissue were labeled with iTRAQ117 and incubated at...

Ngày tải lên: 16/02/2014, 15:20

... R226A and R242A mutants (Table 1), all enzymes were stable for h in the presence of mM potassium phosphate and potassium sulphate Reconstitutions with PLP of apo-Cc StP and mutants thereof, and ... CD and cofactor uorescence emission spectra of native and reconstituted Table Half-lives (t1/2) of CcStP and mutants thereof in the resolution buer at 30 C and pH 7.0 Stable, no inactivation with ... Analytical enzymes and enzyme substrates were specied in previous papers [1518] All other chemicals were of reagent grade and obtained from Sigma and Fluka Preparation of apo-Cc StP and apo-Ec MalP...

Ngày tải lên: 19/02/2014, 16:20

... designated LM1 and LM2, with a mass increase of 55 and 111 Da, respectively, which within the experimental error corresponds to loading of one and two propionyl units, respectively (theoretical mass increase ... fragment also showed two peaks, LM1 and LM2 with mass increase of 67 and 137 Da (theoretical mass increase of 70 and 140 Da, respectively), which corresponds to single and double acylation by the butyryl ... polyketides and polypeptides Masses can be reliably and routinely measured with a precision of 0.01% for strong, wellresolved peaks Even with weak or overlapping peaks the precision of mass measurements...

Ngày tải lên: 16/03/2014, 18:20

... Lee, and Guodong Chen ț Characterization of Impurities and Degradants Using Mass Spectrometry Mike S Lee and Mingshe Zhu ț Mass Spectrometry in Drug Metabolism and Disposition: Basic Principles and ... hallmark of impurity and degradant analysis HPLC is often used to profile and classify molecules and work in concert with mass spectrometry to assist with the elucidation of structure Identification ... an ion and a neutral in free space has the center-of -mass (COM) energy ECOM ECOM ¼ mi mn v mi þ mn R ð1:22Þ TANDEM MASS SPECTROMETRY 31 where mi is the ion mass, mn is the neutral mass, and n...

Ngày tải lên: 28/03/2014, 10:20

... by exact mass measurement and coelution with authentic standards Data processing The raw mass spectrometry data of all samples were processed (deconvolution, alignment, deisotoping and data reduction) ... inferred from mass spectrometry intensities Besides the intensity profile vector xi, also a particular retention time (rt) index and mass- to-charge ratio (m/z) is associated with each marker candidate ... values and a mass shift of m/z 46 The remaining marker candidates of the experiment are represented by small black dots On the right hand side the average intensity profile associated with prototype...

Ngày tải lên: 12/08/2014, 17:20

... Discovery and verification of head -and- neck pre-cancer and cancer biomarkers by differential protein expression analysis using iTRAQ-labeling and multidimensional liquid chromatography and tandem mass ... for Mass Spectrometry, Martinsried, Germany [13,14] The MSM files are peaklists with high precursor mass accuracy and limited product ion ‘noise’ peaks MaxQuant achieves high precursor mass ... Discovery and verification of head -and- neck cancer biomarkers by differential protein expression analysis using iTRAQ labeling, multidimensional liquid chromatography, and tandem mass spectrometry...

Ngày tải lên: 13/08/2014, 13:20

... Yang Lili and Wu Yayun, for their friendship and the happy days we have been together Finally, I would like to thank my parents, my sister and brother for their love, understanding, and patience ... intriguing semantic relations and syntactic features have prompted many scholars debating its particularity and intrinsic properties This study explores the structure, function, and historical developments ... appreciation to my supervisor Shi Yuzhi, with whom I had most hours of classes, independent studies and discussions He has provides me with extensive, impressive and constructive suggestions to my...

Ngày tải lên: 09/09/2015, 17:57