structural disorders of the spinal column

... conformation for the peptide NMR spectroscopy was further utilized to explore the structural properties of Nogo-40 The very narrow resonance dispersion of amide protons ( 0.7 p.p.m) and the lack of side-chain ... attempts to synthesize the C-terminal part of Nogo-40 failed, the peptide Nogo-24, comprising the N-terminal 24 residues of Nogo-66, was successfully produced The sequential assignment of Nogo24 was ... Interestingly, with the introduction of methanol, the CD spectra of Nogo-40 undergo dramatic changes The CD spectra of Nogo-40 in the presence of methanol at a concentration of 74% or above show one positive...

Ngày tải lên: 16/03/2014, 16:20

... is of note that the structure of the LPS O-antigen of F columnare differs structurally from the LPS O-antigen of the fish pathogen Flavobacterium psychrophilium [21], which is a linear polymer of ... component of the O-PS of the fish pathogen Vibrio ordalii serotype O:2 [15], which is the cause of vibriosis among feral and farmed fish and shellfish The only other reported bacterial source of this ... were identical with those of authentic reference glycoses, and further confirms their characterization The combined MS data and the isolation of the two aminoglycoses with the respective D-galacto...

Ngày tải lên: 17/03/2014, 10:20

... the foramen magnum and spinal canal to compress the spinal cord, consequently causing blockage of the spinal canal and the stoppage of cerebral spinal flow, leading to syringomyelia [13,14] The ... was compressed upwards, the pons and cervical spinal cord appeared notch-like due to the compression of the cyst The spinal cord was thickened from the pons to the thoracic spinal cord T10, and ... cystic structure The tissue was sent for pathological examination However, the cyst was not completely excised due to the tight adhesion of the anterior wall of the cyst to the cervical spinal cord...

Ngày tải lên: 25/10/2012, 11:00

... amount of time At the end of the heat treatment the mixtures were placed in an ice/water bath, and the enzymatic activity was stopped by lowering the pH of the reaction mixture to by addition of ... during the thermal treatment, hydrolysis of BLG was extensive, in spite of significant inactivation of the enzymes This indicates that the transient conformers originating in the course of thermal ... in these preparations could stem from the inability of the hydrolytic reaction to address all the sequential epitopes even in the denatured protein In fact, despite the apparent simplicity of the...

Ngày tải lên: 21/02/2014, 15:20

... 2001 cleavages of the sites were in the order: Tyr146/Asn147 ! Phe114 ! other sites The weakness of bands of fragments I and IV and the appearance of fragment II in the degradation of the Tyr146 ... in the rate of cleavage of the autolysis loop At the same time the amount of fragment III was similar to that observed in the degradation of D-chymotrypsinogen (panel a) showing that the rate of ... cleavage was the same as seen from their inactivation rates in the presence of equimolar amounts of trypsin (Table 2) The cleavage of the interdomain and autolysis loops To follow the autolysis of D-chymotrypsin...

Ngày tải lên: 22/02/2014, 07:20

... activity of the enzyme was determined using the Betamyl b-amylase test reagent (Megazyme, Wicklow, Ireland) To determine the pH stability of the b-amylase, aliquots of a solution of the purified ... for h at 25 8C Then 0.1 vol of a solution of 0.5 M sodium acetate (pH 5.0) was added and the activity of the enzyme was measured by the dinitrosalicylic acid method Inhibition of the enzyme activity ... on a column of immobilized b-amylase or CalsepRRP Coupling of the antigens to the column and purification of the antiserum were performed as described previously [28] Western blot analysis The...

Ngày tải lên: 22/02/2014, 07:20

... value of the mutant TNSALP was less than one-tenth of the Kcat ⁄ Km value of the soluble form of the wild-type enzyme, indicating that the replacement of valine with alanine at position 406 in the ... of TNSALP In support of our hypothesis, the cysteine residue at position 433 in one subunit of TNSALP (R433C) becomes crosslinked to the counterpart of the other subunit [29], implying that the ... containing the SV40 early promoter [11] Shortage of a precursor of GPI in the endoplasmic reticulum may be one of the reasons why a small but significant fraction of the wild-type TNSALP forms the aggregate...

Ngày tải lên: 07/03/2014, 05:20

... parameters, there is no relationship between S2 and the location of structural elements (Fig 4) Furthermore, the Rex terms are distributed throughout the 3D structure of the protein, and most of them ... Furthermore, the internal motions of each bond vector are independent of each other, but the rotational diffusion of the molecule affects each of those bond vectors identically [42,43] On the other ... responsible, from a thermodynamic point of view, for the binding of the monomeric species of CAC? We have previously discussed the variation in the free energy of binding as a function of the changes...

Ngày tải lên: 07/03/2014, 06:20

... alignment of Cyn d 24 (A) Nucleotide and deduced amino acid sequences of Cyn d 24 The numbers on the right of the figure indicate the positions of the nucleotide sequence The numbers on the left of the ... Formation of this type of oligosaccharide in BG60 has been suggested to be the result of degradative reactions, rather than imperfect biosynthesis [13] Of the plant allergens listed in the of cial ... to 230 proteins and about 65 of these are IgE binding proteins [20]; however, some of these are known to be either isoforms or the degraded products of allergens Furthermore, allergic patients...

Ngày tải lên: 07/03/2014, 12:20

... the total number of charges measured by electrospray MS Theoretical mass is the mass calculated on the basis of the protein amino-acid sequence The relative abundance refers to the ratio of the ... acetylation at one of the three residues, because the signal of the triacetylated species was less intense than that of the diacetylated species The MS/MS Ó FEBS 2003 Structural properties of SV-IV (Eur ... the mass increase of 42 mass units The relative level of acetylation of a peptide was estimated on the basis of the intensity ratio of the native and acetylated species From the data summarized...

Ngày tải lên: 07/03/2014, 14:20

... for further study The concentration of the proteins was determined by the method of Lowry et al [14] and the purity was examined by SDS/PAGE [15] Assay of human PKIb activity The activity of purified ... regulation of the PKI and PKI isoforms of the inhibitor protein of the cAMP-dependent protein kinase J Biol Chem 272, 20011–20020 Byler, D.M & Susi, H (1986) Examination of the secondary structure of ... 2) The assay of its activity demonstrated that the purified PKIb inhibited the catalytic subunit of cAPK with the specific activity of 6.0 · 104 unitÆmg)1 (Fig 3A) .The Ki value determined from the...

Ngày tải lên: 07/03/2014, 15:20

... Representation of the full-length ERp57 structure with the region recognized as the x-linker colored in red (B) The N-terminal part of the x-linker folds against the b¢ domain in the structures of the ERp57 ... conformations in the structures of the isolated b¢ domain and full-length proteins In the crystal structure of the b¢x fragment of human PDI, the C-terminal part of the x-linker turns back to contact the ... that the C-terminal cap of the substrate binding domains contributes to the specificity of ERp44 How the action of the C-terminal tail of ERp44 is regulated in cells is an intriguing question Structural...

Ngày tải lên: 15/03/2014, 23:20

... fold of one monomer is positioned above the catalytic core of the other Interestingly, Arg267 from the beginning of helix in the HTH fold of one monomer is positioned towards the active site of the ... nucleophilic attack of the a-phosphate of ATP Thus, according to the crystal structure, the specificity of serine recognition depends on: (a) the zinc ion, (b) the size of the active site and (c) the hydrophilic ... that the positioning of the cognate tRNA in the active site of mMbSerRS would be facilitated upon the conformational change of the motif loop, which was the only proximal protein region in the...

Ngày tải lên: 16/03/2014, 06:20

... study the secondary structure of the catalytic domain of PKCf and the effect of its substrate, MgATP, and also to study the effect of thermal unfolding in the presence and the absence of MgATP ... dashed line represents the curve-fitted spectrum, i.e the dotted line is the sum of the individual components consequence of the use of D2O buffer This is the consequence of the H–D exchange that ... structure of 282 residues of a total of 344 have been solved [19] The catalytic domain of PKCh possesses a high degree of homology ( 80%) with the 3280 catalytic domain of other isoforms of PKC...

Ngày tải lên: 16/03/2014, 13:20

... the formation of Hg7-bMT Comparison of the three sets of CD data indicates that the degree of chirality of the Hg-bMT species is generally independent of t However, the chirality of the species ... 2004 Fig Role of the chloride anion in the degree of folding of Hg-MT species observed by comparing the CD spectra of the Hg11-MT species obtained in the titration of Zn7-MT with either HgCl2 (in ... three sets of data indicates that the stoichiometry of the species formed along the three titrations at pH depends on neither the stabilization time, t, nor the nature of the counter-ion The unique...

Ngày tải lên: 16/03/2014, 18:20

... cloning of the gene encoding the secreted CM from Yersinia pestis (*YpCM, y2828), purification of the protein, investigation of the properties of the enzyme, and the crystal structure analysis of the ... the tail of one protomer to almost reach the active site of the neighbor Active site of 90-MtCM The structure of EcCM includes the TSA, which clearly identifies the active site From structural ... 103) O Glu59 (Glu67) Fig The active site of 90-MtCM: a diagrammatic view of the active site of 90-MtCM is shown, with the superimposition of the TSA from the EcCM structure The corresponding residue...

Ngày tải lên: 17/03/2014, 17:20

... (CDRs), are supported by the 2678 structurally rigid b-sandwich framework of the paired variable domains of the light and heavy chains These CDRs come together at the tips of the Y-shaped molecule ... Superposition of Lipocalins Tlc NGAL Fig Molecular architecture of human lipocalins and structural variability of their binding sites Ribbon representation of the crystal structures of four human ... digoxigenin are bound at the bottom of the cleft that harbours biliverdin IXc in the BBP [24] Thus, while the overall topology of the lipocalin, comprising the b-barrel with the a-helix attached to it,...

Ngày tải lên: 23/03/2014, 07:20

... for formation of short helices, transiently, and the chain as such is highly dynamic Structure of the core of the oligomers Because of the large size of the core of the oligomer, the NMR spectra ... assembles in the form of rings around the necks of budding vesicles, and then a conformational change in the dynamin collar aids the scission of the vesicle from the parent membrane The coiled-coil ... signals from the 392 interior of the core and thus not give any information on its structural details Nevertheless, we did derive useful insights into the structural aspects of the core of the oligomer...

Ngày tải lên: 23/03/2014, 11:20

... stainless steel needle (27 g) was butted against the low dead volume tee and enabled the delivery of the sheath solution to the end of the capillary column The separations were obtained on  90-cm long ... in most of 1D analogues of 2D NMR experiments The 1D 1H-NMR spectrum of CPS showed three resonances in the low-field region (5.3–4.4 p.p.m.) in the relative Ó FEBS 2004 ratio of : : 3, of which ... (Table 1) The NH-2a was assigned on the basis of intraresidue NOE with H-1a The NH-2c was assigned based on the intraresidue NOEs with H-1c, H-2c and H-3c (Fig 3B) The presence of the intraresidue...

Ngày tải lên: 23/03/2014, 13:20

... study is the first to determine the full structure of glycoglycerolipid in thermophilic bacteria Meiothermus spp The structural information will be very useful for further investigations of the mechanisms ... steel needle (27 gauge) was butted against the low dead volume tee and enabled the delivery of the sheath solution to the end of the capillary column The separation was obtained on about 90 cm ... were removed and the amino group was acetylated (B) MS/MS spectra (+ev) revealed the sugar sequence of the tetraglycosyl glycerol Fig The 500 MHz spectra of 1H NMR and 1D TOCSY of the N-acetyl tetraglycosyl...

Ngày tải lên: 23/03/2014, 13:20