stromal androgen receptor in prostate cancer

... cell line and clinical RNA sequencing datasets and analysed the overlapping protein-protein interaction network that shared the same pattern of behaviour in both datasets Mechanisms bypassing androgen ... a protein interaction network by taking the set of genes differentially expressed in both datasets, and collecting all protein interactions involving the products of these genes by querying the ... steroid receptor family within the nuclear receptor superfamily Of the 48 nuclear receptors [33] known in the human genome, the sub family of Fig 6 PGR protein expression in prostate cancer cell line,

Ngày tải lên: 22/09/2020, 23:34

... binding sites than HIF binding sites, demonstrating androgen signaling dominance over HIF signaling in the prostate cancer cells studied Interestingly, hypoxia decreased the number of AR binding ... Immunohistochemistry staining was scored by two inde-pendent blinded assessors as 1 (negative), 2 (< 25% of nuclei staining), 3 (25–50% of nuclei staining), 4 (major-ity of cells – weak staining), 5 (majority ... normoxia (86%) in-creased the number of HIF binding sites, the greatest number of HIF binding sites was observed in cell treated with combined hypoxia and androgen treatment (Table I) HIF binding sites

Ngày tải lên: 30/05/2020, 21:31

... staining was present ‘Strong’ was assigned to all tumors showing intense, dark brown staining. ‘Weak’ or ‘moderate’ was assigned to cancer showing staining intensities in between; e.g as shown in ... HER2immunostaining High levels of PTPN12 staining were linked to increased cell proliferation as determined by the Ki67-labeling index (Ki67LI) The average Ki67LI increased from 1.82 in PTPN12 negative cancers ... regarding the ERG status using IHC and FISH was obtained in 95.4% of cases PTPN12 immuno-staining was more prevalent in ERG fusion positive than in ERG wild type cancers PTPN12 immunostaining

Ngày tải lên: 17/06/2020, 18:29

... been the role of insulin metabolism and hyperinsulinemia in prostate cancer progression In cells that express both the insulin receptor (IR) and insulin-like growth factor-1 receptor (IGF-1R), ... cell surface receptor (aka MET kinase), are well de-scribed in progression and invasion of several tumor types, including prostate cancer [33] Met inhibitors such as the tyrosine kinase inhibitor ... IGF-1R/IR receptors are formed, and these hybrid receptors are activated by insulin-like growth factor-1 (IGF-1) as well as likely insulin [10] In a murine model, diet induced hyperinsulinemia has

Ngày tải lên: 20/09/2020, 17:50

... 3blinded to the clinical parameters The intensity of stain-ing was evaluated subjectively on a scale of 0–3, where 0 = no staining, 1 = weak equivocal staining, 2 = un-equivocal moderate staining ... NF-κB binding site and one with a mutated CREB binding site In addition, three specific inhibitors were used to investigate the roles of these proteins in transcriptional activation of Nurr1, including ... shRNA-NLK transfection into LNCaP cells (Fig 4) We also used three inhibitors, including BAY 11–7082 (NF-κB inhibitor), KG-501 (CREB inhibitor) and ICG-001 (CREB binding protein, CBP, inhibitor) Figure

Ngày tải lên: 21/09/2020, 09:46

... inhibitory in lung cancer cells, yet it is also playing a fundamental role in lung cancer metastasis indicating that this integrin func-tions in a complex manner in cancer presumably de-pending ... Background: The EphB4 receptor tyrosine kinase is overexpressed in many cancers including prostate cancer The molecular mechanisms by which this ephrin receptor influences cancer progression are ... integrin clustering and in some cases, inhibit integrin downstream signaling [19,20] Furthermore, ephrin-A1 and EphA2 have both been shown to co-localize with integrin α3 [20-22] In breast cancer

Ngày tải lên: 30/09/2020, 11:10

... expression in prostate cancer cell lines and in clinical PCa tissue (A) Real time PCR analysis of PTPRR expression in 7 prostate cancer cell lines The LNCaP cell lines used were: androgen responsive ... Zecchini V, Scott HE, Lamb AD, et al The androgen receptor induces a distinct transcriptional program in castration-resistant prostate cancer in man Cancer Cell 2013;23:35 –47. 5 Mills IG Maintaining ... Kinase, Androgens, Prostate cancer Background Prostate cancer (PCa) is the most commonly-diagnosed malignancy in men [1], and is driven by androgen hor-mones acting via their cognate nuclear androgen

Ngày tải lên: 30/09/2020, 13:38

... sequences In order to establish consistency and to minimize the risk of contamination during the proce-dure, three individuals independently performed the nested PCR experiments using independently ... samples were heat inactivated at 56°C for 30 min A mixture of plasma samples and 2.5 × 104 infectious units of GFP-carrying XMRV were incubated at 37°C for 30 min before infecting 293T cells (5 ... positive staining was observed in control uninfected 293T cells (B) Serial tissue sections from qPCR positive samples, including #51 (Gleason score (GS) 8) and #103 (GS 6) were immunostained with

Ngày tải lên: 13/08/2014, 01:20

... sensitivity to the tyrosine kinase drug Dasatinib in prostate cancer cell lines, revealing a possible Dasatinib efficacy signature in prostate cancer </p> Abstract Background: Dasatinib is a potent, ... heterogeneity of prostate tumors and the difficulty in obtaining biopsies from prostate cancer patients It remains to be determined whether the molecular subtypes of prostate cancer identified ... basal cells exists in prostate tumors and may play a major role in determining tumor malignancy We found in our study that LNCaP cells, an androgen-sensi-tive prostate cancer cell line, exhibited

Ngày tải lên: 14/08/2014, 08:20

... 12Detecting cancer-specific peptides in prostate cancer INTRODUCTION BACKGROUND OF PROSTATE CANCER Prostate cancer is the 5th most common male cancer in Singapore, affecting 7.2% of all male cancers ... for prostate cancer in Singapore Trang 14Detecting cancer-specific peptides in prostate cancer Prostate cancer is also the most common non-cutaneous male cancer in North America, affecting one in ... six-fold increase in tetramer staining intensity, as compared to minimal background staining with the control DR0401 binding peptide VP16472-484 In the control subject, no tetramer staining was

Ngày tải lên: 05/10/2015, 18:59

... already pre-existing intrinsic refractoriness (4-6) In line with these observations first clinical studies in PCa using anti-angiogenic drugs showed disappointing results, thus calling for a better ... Diverse functions of IGF/insulin signaling in malignant and noncancerous prostate cells: proliferation in cancer cells and differentiation in noncancerous cells Endocrinology 2012;153(10):4633-43 ... (33) In line with these findings, also in the present study only 16/95 patients developed a BCR, thus limiting the explanatory power and statistical significance of this interesting finding Nevertheless,

Ngày tải lên: 15/01/2020, 02:37

... AT in prostate cancer patients, determined that AT provided no benefit, and could promote cancer Conversely, GT3 has shown antineoplastic properties in several in vitro studies, with no clinical ... GT3 in both androgen-sensitive and androgen-independent prostate cancer cells and its multipronged effect on crucial cell signaling pathways af-fecting proliferation and survival Methods Cell lines ... repre-senting standard deviation Results GT3 inhibits cell viability in a time and dose dependent manner in sensitive LNCaP and androgen-independent PC-3 prostate cancer cells, but not AT Re-cent findings

Ngày tải lên: 30/05/2020, 21:49

... of prostate cancer-specific mortality in Pennsylvania remains unclear We utilized advanced geospatial survival regressions to examine spatial variation of prostate cancer-specific mortality in ... factors, including spapoten-tial heterogeneity that may be associated with PC-related mortality in PA According to the North American Association of Cen-tral Cancer Registries (including the U.S ... counties in the Metro (Urban), Appalachian region, and 30 counties in the Rural, Appalachian region. In particular, the PSCI Catchment area includes 28 counties (within a black boarded line) in Central

Ngày tải lên: 30/05/2020, 22:01

... Staining was scored in accordance with the percentage and inten-sity of staining The scores of staining percentage were as follows: 0 (≤5%), 1 (5–30%), 2 (30–70%), 3 (≥70%); the scores of staining ... SLC6A1 in prostate cancer cells, the SLC6A1 coding sequence cloned into pLV.O(Pro-vided by Huiyuanyuan company of China)/ blank vector (NC) were transfected into prostate cancer cells using Lipo-fectamine ... progression and poor prognosis in patients with prostate cancer As shown in Fig 1a-b, positive immunostaining of SLC6A1 protein was localized in the cell cytoplasm of prostate cancer tissues The median

Ngày tải lên: 17/06/2020, 11:37

... improve the individuals’ psychological and social functioning, including the ability to return to work Our findings highlight that RTW in prostate cancer survivors who were active in the working force ... radical prostatec-tomy [47] Fig 2 Return to work (RTW; in days) of prostate cancer survivors within the 12 months following the cancer rehabilitation program ( N = 549) Trang 9Interestingly, the ... days in U.S prostate cancer patients [11] In a study with Norwegian prostate cancer patients who were employed before radical prostatectomy, 51% had returned to work within 6 weeks and 73% within

Ngày tải lên: 03/07/2020, 03:00

... incu-bated in pretreatment solution at 80 °C for 35 min, rinsed for 3 min in deionised water, incubated 10– 20 min in 0.15% pepsin in 0.1 N HCl solution at 37 °C, and rinsed again for 3 min in ... margin, indicating a field cancerisation effect in prostate cancer [40] This characteristic may be beneficial to reduce sampling error and consequently the risk of missing a higher-grade cancer ... researchers from participating institutions KPNC Trang 10investigators were responsible for the final data presentation in themanuscript, data interpretation, clinical opinion, and final manuscript review.

Ngày tải lên: 23/07/2020, 23:51

... for nc886 in the prostate, whose expression is epigenetically silenced in cancer leading to an increase in cell proliferation and invasion Nc886 might hold clinical value in prostate cancer due ... methylation increases in tumor vs normal prostate tissue, as well as in metastatic vs normal prostate tissue Additionally, nc886 promoter methylation correlates with prostate cancer clinical staging, including ... Public Domain Dedication waiver Trang 2Prostate cancer (PrCa) is the solid tumor with the highest incidence in men in Western countries, representing the second leading cause of male cancer death

Ngày tải lên: 24/07/2020, 00:42

... Background: There is increasing appreciation that non-cancer cells within the tumour microenvironment influence cancer progression and anti-cancer drug efficacy For metastatic prostate cancer (PCa), ... inserts were immersed in crystal violet stain (0.5%, diluted in H2O) for 15 min Transwell inserts were washed in running tap water to remove excess stain Crystal violet stain was extracted The ... maintained at 37°C and 5% CO2 PCa cell Transwell migration assay We were also interested in determining if PCa cell migration towards stromal cells differed depending on the geometry of the stromal

Ngày tải lên: 24/07/2020, 01:02

... Results: ERCC1 staining was seen in 64.7% of 10,436 interpretable tissues and was considered weak in 37.1%, moderate in 22.6% and strong in 5% of tumors High-level ERCC1 staining was linked to advanced ... weak in 37.1%, moderate in 22.6%, and strong in 5% of tumors Representative images of ERCC1 immunohistochemistry in prostate cancer samples are shown in Fig 1 Strong ERCC1 staining was linked ... going beyond architecture and also involving digital image analysis and deep machine learning will play a very important role in prostate cancer assessment in the future Conclusions strongly linked

Ngày tải lên: 06/08/2020, 06:28

... distinct functional domains, namely, a N-terminal domain (NTD) containing transcriptional activation units (AF-1 and AF-5), a DNA binding domain (DBD) where four-cysteine zinc-binding domains ... Defined Role of ETS proteins in Prostate Cancers (Kumar-Sinha et al., 2008) 28 1.9 Reduced Androgen Signaling in Advanced Metastatic Prostate Cancers A partial attenuation of androgen signaling ... 1.7.2 The Androgen Response Elements and other Motifs in ARBS Like other DNA binding transcription factors, the AR DNA binding domain is mainly responsible for determining its DNA binding specificity...

Ngày tải lên: 09/09/2015, 10:14