stem cells in regeneration and therapy

... stem cells is considered an important approach in cancer treatment However, the similarity between stem cells and cancer stem cells can cause mistargeting between stem cells and cancer stem cells, ... of DCs and induced cytotoxic T lymphocytes (CTLs) in vitro and in vivo Materials and methods 4T1 culture Murine 4T1 mammary gland tumor cells, which are sponta-neously metastatic tumor cells derived ... cancer stem cells or stem cells This study evaluates the specificity of DC therapy primed with breast cancer stem cells (BCSCs) in breast cancer treatment We investigated the specific inhibi-tion

Ngày tải lên: 16/12/2017, 00:33

... hours (h), at low and high subculture passages The hASCs are commonly used in cell-based therapy since their isolation is minimally invasive and because they are abundant and rapid in proliferation ... (Gibco) and 1% Penicillin-Streptomycin Solution Both hASCs and hWJ-MSCs were maintained at standard culture conditions of 37°C with 5% carbon dioxide in a humidified atmosphere The non-adherent cells ... and negative (not colored) cells was counted in each sample in at least three random fields under a light microscope (at 200× magnification and bright field illumination) [68] To avoid staining

Ngày tải lên: 15/01/2020, 02:05

... PKCδ as a target in primary cancer stem cell lines and stem-like cells derived from human tumor cell lines, including breast, pancreatic, prostate and melanoma tumor cells Novel and potent small ... Cancer Institute (Detroit, MI) and maintained in DMEM-F/12 medium containing 5% heat-inactivated horse serum, 10 μg/mL insulin, 20 ng/mL epi-dermal growth factor, 0.1 μg/mL cholera enterotoxin, and ... self-renewing and anchorage-independent sphe-roids were obtained by culturing breast cancer cells MCF7, Hs587T and MDA231; melanoma cells SBcl2 and FM6; human breast cancer stem cells and pancreatic

Ngày tải lên: 05/11/2020, 01:11

... clearest evidence of in vitro oogenesis In addition, the PSCs showed positive alkaline phosphatase staining, and the intensity of the staining was stronger in the germinal granules than in any other ... that germline stem cells per se might not persist in postnatal and adult mammalian ovaries, but that progenitor cells/small PSCs in the ovary can instead differentiate into germline stem cells under ... (HBSS) and 10 min with 0.25% trypsin-EDTA at 38.5°C Trypsin was neutralized by adding 10% fetal bovine serum (FBS), and tissues dispersed into single cells by gentle pipetting The dispersed cells

Ngày tải lên: 16/12/2017, 04:38

... CD24-/CD44+ cells These findings indicate that a distinct CD24+/CD44+ subpopulation may represent CSC or tumor initiating cells in HNSCC We confirmed the stemness feature of CD24+/CD44+ cells by showing ... Sensitivity of CD24+/CD44+ and CD24-/CD44+ cells to cisplatin and gemcitabine anticancer drugs Flow cytometry sorted cells were exposed to cisplatin (A), and gemcitabine (B) at increasing concentrations ... CD29 (β1-integrin) and CD44 expression in HNSCC More than 99% cells were CD29 and CD44 double-positive, indicating CD24+/ CD44+ cells were also CD29+ Recently, a subpopulation of cells (Lin−/CD29+/CD24+)

Ngày tải lên: 05/11/2020, 01:35

... for the invasion-metastasis cas- cade of CSC [240,241] Several different molecules are involved in the homing and invasion process, including selectins and selectin-ligands, integrins and their ... however, stem cells do not undergo redis- tribution and homing, unless these cells transform to metastasizing CSC Stem cell homing of normal hematopoietic stem cells and LSC is a multi-step process and ... to stem cell niche teractions and stem cell self-renewal in normal and (pre) malignant conditions [225]. in-Stem cell homing and abnormal spread of NSC/CSC Depending on the organ system, homing

Ngày tải lên: 02/11/2022, 09:03

... CD24-/CD44+ cells These findings indicate that a distinct CD24+/CD44+ subpopulation may represent CSC or tumor initiating cells in HNSCC We confirmed the stemness feature of CD24+/CD44+ cells by showing ... Sensitivity of CD24+/CD44+ and CD24-/CD44+ cells to cisplatin and gemcitabine anticancer drugs Flow cytometry sorted cells were exposed to cisplatin (A), and gemcitabine (B) at increasing concentrations ... CD29 (β1-integrin) and CD44 expression in HNSCC More than 99% cells were CD29 and CD44 double-positive, indicating CD24+/ CD44+ cells were also CD29+ Recently, a subpopulation of cells (Lin−/CD29+/CD24+)

Ngày tải lên: 02/11/2022, 11:41

... markedly increased the staining intensity of HIF-2α and Oct4, while HIF-2α or Oct4 silencing markedly suppressed it The increase was further improved in the cells co-overexpressing both HIF-2α and ... vselMSCs against the apoptotic response to hypoxic injury. There was high expression of bFGF, VEGF, Bcl2, and survivin mRNA and protein in HIF-2α+vselMSCs and than in siHIF-2α vselMSCs and siOct4vselMSCs, ... function and heart remodeling in the infarcted rats We further demonstrated that HIF-2 α and Oct4 jointly regulate their relative downstream gene expressions, including Bcl2 and Survivin; the

Ngày tải lên: 04/12/2022, 10:38

... delivery investigated a tissue engineering approach where two strategies are mainly applied: engineering of a stem cell-containing tissue construct or a beating cardiomyocyte-containing tissue ... valued for their paracrine effects in reducing inflammation [188] and promoting growth of the surrounding cells [268] MSC injection promotes the recruitment of CPCs and helps in the improvement of ... remain to be addressed. Keywords: Mesenchymal stem cells, Cardiac regeneration, Niche hypothesis, Cell therapy, Cell transplantation Background Stem cells are capable of differentiating into cells

Ngày tải lên: 04/12/2022, 15:30

... spermatogonial stem cells Int J Androl 2000;23(Suppl 2):89–91. 29 McLean DJ, Russell LD, Griswold MD Biological activity and enrichment of spermatogonial stem cells in vitamin A-deficient and hyperthermia-exposed ... Brinster RL Maintenance of mouse male germ line stem cells in vitro Biol Reprod 2003;68:2207–2214. 33 Orwig KE, Avarbock MR, Brinster RL Retrovirus-mediated modification of male germline stem cells ... multiplication and stem cell renewal in the C3H/101 F1 hybrid mouse Mutat Res 1993;290:193–200. 40 Nagano MC Homing efficiency and proliferation kinetics of male germ line stem cells lowing transplantation

Ngày tải lên: 10/08/2014, 18:21

... 1 Schematic for preclinical testing of stem cell-based therapies Preclinical testing of therapies derived from stem cells should be initiated in vitro and include genotype and phenotype assessments ... understand and define the risks and the benefits of stem cells transplantation before clinical trials in humans 5 LENGTH OF PRECLINICAL STUDIES Determining the appropriate length of the preclinical ... using stem cell-derived cellsfor in vivo testing 3.2 In Vivo Testing After the functional capacity of cells destined for transplantation have beenestablished with in vitro systems, cell safety and

Ngày tải lên: 10/08/2014, 18:21

... apoptotic and inflammatory sig-nalling pathways, promoting co-culture induced chon-drogenesis Curcumin suppresses IL-1β-induced apoptotic and inflammatory responses in MSCs in a time and concentration ... (OA) and rheumatoid arthritis (RA) involve degenerative changes in the joint, leading to loss of function, pain and significant disability [1] OA and RA are not only common joint diseases in the ... IL-1β alone and was blocked in all cultures treated with IL-1β and curcumin Here, a four-hour pre-treatment with curcumin was as effective in inhibiting the IL-1β induced apoptotic signalling cas-cade

Ngày tải lên: 12/08/2014, 14:22

... levels of proinflammatory cytokines and chemokines (TNF-a, IL-6 and monocyte chemoattractant protein-1) and increased levels of the anti-inflammatory/regulatory cytokine (IL-10) in sera of UC-MSCs ... reduced inflammatory responses in CIA There were reduced levels of proinflammatory cytokines and chemokines (TNF-a, IL-6 and MCP-1) and increased levels of the anti-inflammatory/regulatory cytokine ... responses and induced Tregs in CIA Conclusions: In conclusion, human UC-MSCs suppressed the various inflammatory effects of FLSs and T cells of RA in vitro, and attenuated the development of CIA in

Ngày tải lên: 12/08/2014, 15:22

... also seen in primitive endoderm and extraembryonic endoderm cell lines (XEN cells) [29,30], and can be induced in ESCs or in the inner cell mass (ICM) by knocking out or downregulating expression ... (NCAM) and cadherin 11 (Cdh11), which are expressed in MSCs, were not expressed in MSC-like cells, and Nrcam, Pdgfra, CD24, and Cdh13 were expressed in MSC-like cells but not in MSCs One thousand ... murine MSCs, and Sca-1 and CD44, which, as mentioned above, discriminates between mMAPCs and MSC-like cells Hence, although some differences could be detected in MSCs and MSC-like cells, they involve

Ngày tải lên: 14/08/2014, 08:20

... embryonic stem cells into ovarian structures containing oocytes Stem Cells 2006; 24:266–273 24 Qing T, Shi Y, Qin H, et al Induction of oocyte-like cells from mouse embryonic stem cells by co-culture ... pivotal role in translation initiation by binding eIF4E and eIF4A, by forming a bridge between the mRNA and the ribosome, and by binding PABPC1 to facilitate translation of poly(A)-containing mRNAs ... stored mRNAs RINGO/Spy protein is absent in the oocyte cytoplasm, while its mRNA is maintained in a dormant state This is achieved by binding of Pumilio-2, an RNA-binding protein initially described...

Ngày tải lên: 22/03/2014, 20:21

... marrow injury conditions [43], subsequent studies have expanded this finding to situations of inflammation [44], and peripheral tissue injury [45-47] Hematopoietic stem cells are being increasingly ... a peak increase of approximately 53% in the number of CD34 expressing cells and and a 90% increase in CD133 cells in circulation Furthermore, a significant augmentation of cells possessing hematopoietic ... medium containing 100 U/ml penicillin, 100 μg/ml streptomycin, and mM L-glutamine, and subsequently resuspended in 100 ul (0.5 M cells per 100 ul) of buffer (PBS+0.5% BSA) Cells were stained with...

Ngày tải lên: 18/06/2014, 16:20

... hematoxylin and eosin, and then with combined Luxol fast blue and cresyl violet to identify myelin and nerve cells [10] Percentages of myelinated areas in damaged spinal cords were calculated using ... stained with Alizarin red S D: Intense Alizarin red S stained after weeks incubation at osteogenic media and confirmed calcium deposition A and B: Oil red O stain, C and D: Alizarin red S stain, ... effective in canine SCI model, there was little histological evidence of spinal cord tissue regeneration [22] In this study, we examined whether canine ASCs could survive and integrate into neural cells...

Ngày tải lên: 07/08/2014, 23:22

... such as intestine [52] and from the ectodermal tissues including skin [53], deciduous teeth [54] and nerve tissue [8,9,55,56] ASCs originate during ontogenesis and remain in a marginal area in a ... islets and reintroduce physiological secretion of human insulin AHSCT improves b -cells function and frequently decreases the exogenous insulin need [20] or induces a persistent insulin independence ... enlargement and blood clots in veins and arteries Surgery is the usual therapy and, in many cases, is followed by chemotherapy [234-236] The gastrointestinal tract is a target of GVHD in transplants and, ...

Ngày tải lên: 10/08/2014, 10:20

... expand and differentiate [6] Their origin could be in embryonic cells or in progenitors unrelated to embryonic stem cells Adult stem cells are undifferentiated cells occurring in tissues and ... capacity [42] Induced pluripotent stem cells Induced pluripotent stem cells (iPSCs) from somatic cells are revolutionizing the field of stem cells Obtained by reprogramming somatic stem cells of a ... minimizing the risk of rejection ASCs regulate T cell function by promoting induction of suppressor T cells and inhibiting production of cytotoxic T cells, NK cells, and proinflammatory cytokines...

Ngày tải lên: 18/06/2014, 16:20

... and HepG2/ADM cells The drug-resistant HepG2/ADM cells were cultured in the cell culture medium containing μg/ mL adriamycin (Sigma) Both cell lines were maintained in RPMI-1640 medium containing ... mice using a 1.0 mL syringe Intravenous injection of reagents and tumor growth inhibition study The nude mice inoculated with HepG2/ADM cells were divided into four groups with seven mice in each ... DNR-mediated apoptosis in HepG2r/ADM cells, we investigated apoptosis-related protein expression in the cells (Figure 6C) DNR or Cdte QDs cannot induce apoptosis strongly in HepG2r/ADM cells due to multidrug...

Ngày tải lên: 21/06/2014, 03:20