stem cell biology and research impact factor

... embryonic stem cells during their self-renewal However, after the identification of the protein constituents of a given system, the next step usually involves understanding how these systems change ... to Stem Cell Biology and Regenerative Medicine Edited by Sheng Ding Copyright
2008 John Wiley & Sons, Inc 145 146 PROTEIN CHARACTERIZATION BY BIOLOGICAL MASS SPECTROMETRY biological systems Since ... of stem cell biology The various references cited are clearly not meant to be exclusive, but rather provide good starting points for obtaining additional details regarding the numerous topics and

Ngày tải lên: 01/03/2023, 11:31

... CHEMICAL AND FUNCTIONAL GENOMIC APPROACHES TO STEM CELL BIOLOGY AND REGENERATIVE MEDICINE CHEMICAL AND FUNCTIONAL GENOMIC APPROACHES TO STEM CELL BIOLOGY AND REGENERATIVE MEDICINE ... Xu, Simon Hilcove, and Sheng Ding Regeneration Screens in Model Organisms 207 Chetana Sachidanandan and Randall T Peterson 10 Proteomics in Stem Cells 223 Qiang Tian and W Andy Tao Index 243 ... Large-Scale Genomic Analysis of Stem Cell Populations 169 Jonathan D Chesnut and Mahendra S Rao v vi CONTENTS Exploring Stem Cell Biology with Small Molecules and Functional Genomics 187 Julie

Ngày tải lên: 01/03/2023, 11:33

... this thesis, and to Rory Johnson for his feedback and comments I also wish to thank everyone in the GIS Stem Cell group for stimulating discussions and fun companionship Finally and most importantly, ... in ES cells 117 3.3.2 Zic3 functions downstream of Oct4, Nanog and Sox2 and is positively regulated by 4.2.3 Zic3 and Sox2 co-occupy physical binding sites in mouse ES cells 134 4.3.1 Zic3 and ... factor, Zic3, in the maintenance of ES cell pluripotency Zic3 is expressed in ES cells and this expression is repressed upon differentiation The binding of transcription factors Oct4, Nanog and

Ngày tải lên: 11/09/2015, 09:02

... Transcriptional control of stem cell pluripotency 1.1.1 The transcription factors OCT4, NANOG and SOX2 constitute the human embryonic stem cell transcriptional core 1.1.2 An expanded transcriptional ... in the GIS Stem Cell groups for companionship, support and helpful discussions Most importantly, my family has given me incredible support and encouragement Their love and understanding helped ... pluripotent stem cells 28 3.2 Expansion and mitotic inactivation of MEF cells 28 3.3 Preparation of MEF-conditioned medium 29   iii  3.4 Culture of PA6 mouse skull bone marrow stromal cells 29 3.5

Ngày tải lên: 09/09/2015, 17:54

... pluripotent stem cells and a panel of somatic tissues Of these, AK056826 (lncRNA_ES1), EF565083 (lncRNA_ES2) and BC026300 (lncRNA_ES3) were specifically expressed in the three pluripotent stem cell ... in NPCs compared to hESCs and neurons, and mirrored the expression of genes known to be important in the maintenance of neural stem cell identity, such as PAX6, POU3F2 and SOX1 (Figures 5.2C-D) ... Pluripotency and Neural Differentiation LncRNAs are emerging players in embryogenesis and in developmental processes (Amaral and Mattick, 2008; Dinger et al., 2008) Recent studies in embryonic stem cells

Ngày tải lên: 09/09/2015, 17:54

... confirmed by qPCR in two independent cell lines: hESC (H1)-derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression was upregulated ... factor, is a transcription factor expressed in neural stem cells and non-neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds upstream of RMST in the neural stem ... AK056164, AF429305 and AF429306 (Figure 7.1 and Figure 8.4) In humans, RMST is located on chromosome 12q23, and the ENCODE ChIP-seq data from human embryonic stem cells (H1) and neuroblastomas

Ngày tải lên: 09/09/2015, 17:54

... hnRNPA2 and SOX2 Fold enrichment relative to the isotype IgG RIP is presented, and the respective p-values are indicated (A) hnRNPA2-FLAG was ectopically expressed in ReN-VM cells, and enrichment ... RMST or SOX2 in neural stem cells Figure 8.18: RMST and SOX2 regulate a common pool of targets The extent of overlap between differentially expressed genes in si-SOX2 and si-RMST datasets was ... hnRNPA2B1 and transcription factor SOX2 In addition, the data presented in this chapter indicates that RMST and SOX2 regulate a large set of target genes that are essential for neurogenesis, and supports

Ngày tải lên: 09/09/2015, 17:55

... as in the regulation of intracellular calcium signals, in angiogenesis and control of cell adhesion molecule expression, and chemotaxis More particularly, in immune cells, it was shown that the ... (S1P), the goldstandard method to measure and quantify SPHK activity in vitro More details about this assay will be addressed below 2.1.2.1.3 Cell lysis CHO cells were collected and re-suspended ... mixed and left to stand at room temperature for 5-10 minutes The organic and aqueous phases were separated by adding 240μl of chloroform and 240 μl of 2N KCl The mixture was then vortexed, and

Ngày tải lên: 11/09/2015, 16:06

... Inhibitors in Human BM- and AD- MSCs Differentiation CHAPTER ROLES OF SPHK INHIBITORS IN HUMAN BMAND AD- MSCS DIFFERENTIATION Human stem cells are more and more becoming the research focus for their ... promote stem cells differentiation in order to develop novel and safer strategies for stem cells differentiation In our study, inhibition of the enzyme SPHK, which is tightly related to the cells ... when suppressing SPHK activity in the stem cells, the balance between proliferation and differentiation could be lost, the cell growth was arrested, and the cells would then enter into certain

Ngày tải lên: 11/09/2015, 16:06

... PDGF and promote human embryonic stem cells Trang 2proliferation, as well as maintaining their stemness However, human adult stem cells, such as MSCs, are very different with embryonic stem cells ... the stem cells remained as stem cells Starting cells for human BM-MSCs were passage 6, and for human AD-MSCs (cells from two different donors) were passage 4 Cells were equally grouped and cultured ... It can act as an intracellular mediator, and it can also act as an extracellular ligand to bind and stimulate its-specific receptors, expressed on the same or nearby cells, in an autocrine or

Ngày tải lên: 11/09/2015, 16:06

... Heart J 146(1): 62-8 Dimarakis, I and N Levicar (2006) "Cell culture medium composition and translational adult bone marrow-derived stem cell research." Stem Cells 24(5): 1407-8 Djouad, F., ... "Mesenchymal stem cells rescue CD34+ cells from radiation-induced apoptosis and sustain hematopoietic reconstitution after coculture and cografting in lethally irradiated baboons: is autologous stem cell ... hematopoietic stem cells from normal human bone marrow." Stem Cells 1(2): 111-23 Kolesnick, R and D W Golde (1994) "The sphingomyelin pathway in tumor necrosis factor and interleukin-1

Ngày tải lên: 11/09/2015, 16:06

... one hand, it could act as an intracellular mediator and trigger numerous cellular events, including intracellular calcium release, and promote cell survival and cell growth On the other hand, ... Therefore, mediating stem cells differentiation, in particular, promoting stem cells differentiation, in a controlled way, would be highly desirable in stem cells research and in their future ... BM-MSCs and AD-MSCs, as tissue-specific stem cells, are very different from human embryonic stem cells in terms of cell differentiation properties and in vitro culture conditions Human BM-MSCs and

Ngày tải lên: 11/09/2015, 16:06

... MICRORNAS IN EMBRYONIC STEM CELL DEVELOPMENT AND DIFFERENTIATION TAY MEI SIAN YVONNE NATIONAL UNIVERSITY OF SINGAPORE 2008 THE ROLE OF MICRORNAS IN EMBRYONIC STEM CELL DEVELOPMENT AND DIFFERENTIATION ... providing me with an excellent environment to hone my technical expertise and intellectual prowess, and giving me the opportunity, independence and resources to conduct my research. His tremendous ... Seng, Huibin, Yin Loon, Minh, Sandy, Phil and all other past and present members of Bing’s lab, thank you for your friendship, and for making the lab such a stimulating and fun place to work in.

Ngày tải lên: 12/09/2015, 08:16

... minority of cancer stem cells These cancer stem cells possess tumour-related features such as uncontrolled growth and the ability to form metastases They also maintain their inherent stem cell capacity ... Clarke MF Cancer stem cells: models and concepts Annu Rev Med 2007; 58: 267-84 Dallas NA, Xia L, Fan F, et al Chemoresistant colorectal cancer cells, the cancer stem cell phenotype, and increased ... cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals Cell 1994; 78(1): 59-66 Polyak K, Hahn W Roots and stems: stem cells in cancer Nat Med 2006; 12(3):

Ngày tải lên: 03/10/2015, 20:57

... stem cells, can-cer stem cells, and potential of stem cells engineering to revolutionize cancan-cer treatment Keywords— Stem Cells, Cancer, Stem Cell therapy, Stem cell treatment, Cancer stem cells ... CSCs: Cancer stem cells; DC: dendritic cells; MSCs: Mesenchymal stem cells; SCT: stem cell transplant; iPSCs: induced pluripotent stem cells Sincerely thanks to Jeffrey J Heard and Ashley L ... therapy, immunotherapy, and stem cells Stem cells have contributed significant roles for those approaches of cancer treatment and research Basing on unique characteristics of stem cells such as self-renew,

Ngày tải lên: 16/12/2017, 14:59

... metastasis and a high incidence of chemo- and radio-resistant whether the expression of AHR and its downstream target CYP1B1 drives cellular mechanisms associated with poor IBC prognosis and the stem cell-like ... AHR ligands induce breast cancer stem-like cells in human triple-negative breast cancer (TNBC) and IBC[11] cells Furthermore, correlations were found between the AHR signalling pathway and markers ... hyper-activation of AHR and after AHR knock down in SUM149 IBC cells Since AHR and CYP1B1 levels correlated with Wnt5a/b and b-catenin in IBC but not in non-IBC (Fig 3E and F) In addition, AHR and CYP1B1

Ngày tải lên: 14/01/2020, 02:39

... 10.7150/ijms.34758 Research Paper MRC-5 Cancer-associated Fibroblasts Influence Production of Cancer Stem Cell Markers and Inflammation-associated Cell Surface Molecules, in Liver Cancer Cell Lines Song-Ming ... proportions of CD90+ cells were significantly higher in MHCC-LM3-(MRC-5)-CM and HepG2-(MRC-5)-CM cells, and moderately higher in Bel-7402-(MRC-5)-CM and SMMC-7721-(MRC-5)-CM cells, than in controls ... MHCC-LM3-(MRC-5)-CM cells, and moderately higher in HepG2-(MRC-5)-CM cells, than controls However, the TLR1+ and TLR4+ proportions were lower in Bel-7402-(MRC-5)-CM and SMMC-7721-(MRC-5)-CM cells than

Ngày tải lên: 14/01/2020, 23:21

... attachmentof some cells (e.g., fibroblasts but not epithelialcells) to extracellular matrix components b. Itbindsantigens and enzymes to the cell surface c. It facilitates cell-cell recognitionandinteraction. ... Nonmuscle Cells 122 Review Test 123 Answers and Explanations 125 Trang 99 NERVOUS SYSTEM 126I. Overview—Nervous System 126 II. Histogenesis of the Nervous System 126 III. Cells of Nervous System 127 ... 1943-Cell biology and histology / Leslie P Gartner, James L Hiatt, Judy M Strum — 6th ed. p ; cm — (Board review series) Includes bibliographical references and index. Summary: “BRS Cell Biology and

Ngày tải lên: 20/01/2020, 04:43

... dermis, and composed of three cell types a Dark cells line the lumen of the gland and contain many mucinogen-rich secretory granules b Clear cells underlie the dark cells, are rich in mitochondria and ... zona fasciculata, and zona reticularis (Figure 13 10) 3 Zona reticularis a synthesizes and secretes weak androgens (mostly dehydroepiandrosterone and some androstenedione) and perhaps small amounts ... neuroendocrine system (DNES) cells, located in the epithelial lining of the respiratory and gastrointestinal systems (discussed in chapters 15 and 16, respectively) B Glands of the endocrine system include

Ngày tải lên: 22/01/2020, 06:06

... clusters of cells between the follicularcells and basal lamina 2. These cells belong to the population of DNES cells,also known as APUD cells(amine precursor uptake and decarboxylation cells), or ... glandsis embedded in the dermis and is composed ofthree cell types a Dark cellsline the lumen of the gland and contain many mucinogen-rich secretorygranules b Clear cellsunderlie the dark cells, ... epitheliumconsisting of olfactory cells, porting (sustentacular) cells, and basal cells sup-(3) It has a lamina propria that contains many veinsand unmyelinated nervesandhouses Bowman glands. LWBK615-c15[228-243].qxd

Ngày tải lên: 22/01/2020, 16:54