species cell lines and animal breeds

... proteins on the cell surface and is regulated at least in part by cellular signaling through cell-cell and cell-ECM interactions Automatic impedance recording began after cell seeding and continued ... in HER2+ and HER2- breast cancer cell lines a HER2+ cell lines (n = 9) and HER2- cell lines (n = 10) were evaluated with the CELx HSF test as described in the Methods The sum of NRG1b- and EGF-driven ... demonstrated using ligands with high affinity and specificity for HER1 and HER3 The HER2-driven signaling quantified ranged 50-fold between the lowest and highest cell lines The HER2+ cell lines were almost

Ngày tải lên: 19/09/2020, 21:32

... melanoma cell lines depending on their origin [18] Methods Cell lines and cell culture conditions The selected cell lines included cells of primary and meta-static origin Thirteen human melanoma cell ... their cell surface However, melanoma cell lines show intracellular expression of all the aforementioned receptors and most of their respective ligands When analyzing the xenografts and the cell lines ... examples are shown in Figure 2 (C and D) and Figure 3 (C and D) Surface and intracellular expression of chemokines and their receptors in WM-115 and WM-266.4 cell lines after xenotransplantation

Ngày tải lên: 05/11/2020, 01:20

... neural crest cells and glioma cells (Dirks, 2001) The idea behind cancer stem cell as an origin for brain tumor arose from the identification of stem cells among leukemic cells These stem cells possess ... al., 2006) Demyelination and gap junctions The etiology of MS and NMO is associated with immune cells (T and B cells), although the initiating cause is still unknown and several contributing ... functional coupling and Cx43 expression of three different glioma cell lines They found that DEX decreases both functional GJC and Cx43 protein expression in all three cell lines (Hinkerohe et

Ngày tải lên: 02/11/2022, 11:39

... SF295 cells were grown in DMEM, and all other cell lines were grown in RPMI 1640 All media contained 10% heat-inactivated fetal bovine serum and antibiotics For cell survival studies, cells were ... making cells more susceptible to radiation-induced DNA damage and, 3) downregulates total Chk1 and Cyclin B abrogating the radiation-induced G2/M checkpoint and allowing cells to re-enter the cell ... repaired, cells are released from the cell cycle block and are allowed to divide Persistent DNA damage may result in cell death initiated by other surveillance mechanisms In eukaryo-tic cells, the

Ngày tải lên: 09/08/2014, 10:20

... time- and concentration- dependent cell death in all cell lines with T98G and A172 cells being more sensitive than U87MG cells at all time points (Fig 1C–E). Human malignant glioma cell lines ... malignant glioma cell lines to radiation-induced cell deathFigure 6 ErPC sensitizes human malignant glioma cell lines to radiation-induced cell death. Induction of apoptosis and clo- nogenic cell survival ... could increase radiation-induced cell death in human malig- nant glioma cell lines. To this end, T98G, A172 and U87MG cells were treated with 2.5, 5 and 10 Gy and/or 0, 12.5, 25, 50, 75 or 100

Ngày tải lên: 09/08/2014, 10:20

... human lymphoblastoid cell lines (LCLs) from the HapMap col-lection These cell lines represent a unique resource as they have been densely genotyped by the HapMap Pro-ject [21], and are now being ... and PolII binding [25,26] Finally, one of the HapMap cell lines is now being intensely studied by the ENCODE Project [27] This convergence of diverse types of genome-wide data from the same cell ... (d) Methylation levels inside and outside of annotation categories, including CpG Islands (CGIs) for probes within 100 bp of the TSS, and histone modifications and transcription factor (TF) binding

Ngày tải lên: 09/08/2014, 22:23

... promising and prospective resources for cell and gene therapy in mesenchymal and non-mesenchymal applications because of their great self-renewal and versatile plasticity in vitro and in vivo ... primary cell cultures of hMSCs with HPV 16 E6E7 and devel-oped the KP cells [6], which were then overexpressed with hTERT Several single-cell derived clones were iso-lated and 3A6, 1C5 and 3G11 ... between 3A6 and KPby performing RT-PCR for trophoectoderm (CDX2 and CGb), germline (Dazl, Vasa and Scp3), osteogenic (BSP, Bone Sialoprotein and OCN, Osteocalcin) and neural (Pax6 and Nestin)

Ngày tải lên: 10/08/2014, 05:21

... cyclinD1, and on cancer cells invasion, proliferation and cell cycle cell in two cell lines were investigated Methods Cell lines and culture The human breast cancer cell lines MDA-MB-231 and MCF-7 ... mentioned Figure 6 Cells growth curve and MTT analysis for MDA-MB-231 and MCF-7 cells A: cells growth curve analysis for MDA-MB-231 and MCF-7 cells B: MTT analysis for MDA-MB-231 and MCF-7 cell compared ... with 2 × 103 cells/filter, and cells were cultured for 24, 48, 72 and 96 hours, respectively, then added 20 ul MTT to cells and cultured for 4 hours After removing the cul-ture medium and adding

Ngày tải lên: 10/08/2014, 10:21

... resistance in breast and ovarian cancer cell lines Niels Eckstein Abstract Breast and ovarian cancers are among the 10 leading cancer types in females with mortalities of 15% and 6%, respectively. ... 13, and 14) and (ii) proa- poptotic caspases. Caspases triggering apoptosis are further categorized into initiating caspases (subtypes 2, 8, 9, and 10) and effector caspases (subtypes 3, 6, and ... uptake, increased efflux, or cellular compartimen- tation. Several ATP binding cassette (ABC) transport pro- teins are involved like MRP2 and MRP6, Ctr1 and Ctr2, and ATP7A and ATP7B, respectively

Ngày tải lên: 10/08/2014, 10:21

... tumor growth and in the induction of T cell dysfunction. Therefore, we studied in murine renal cell carcinoma (RCC) cell lines, the effect of arginase II on tumor cell proliferation and L-arginine ... cell lines expressing arginase II can modulate the L-arginine metabolic pathway to regulate both cell growth and T-cell function. Blocking arginase may lead to a decrease in RCC cell growth and ... CL-19 after 48 and 72 hours (P = 0.001 and P < 0.0001) compared to CL-2 and Renca cell lines in which the levels did not change significantly at any time point (Figure 2B). All cell lines also

Ngày tải lên: 10/08/2014, 22:20

... higher in the P8 cell lines than in the R1 cell lines Release of CAgag protein and MuLV particles from R1A and P8A cell lines and analysis of infectivity levels To determine whether cell-associated ... brains, R1A cell lines, P8A cell lines, and in SC-1 cells which had been infected with P8A cell line homogenate (labeled SC-1-Tf-P8A1), whereas CAgag was not detected in the Zpl 2-1 cell lines, SAMR1 ... SAMR1 brains, and ICR-A cell lines (Fig 5A) The CAgag levels in P8B cell lines were significantly higher (p < 0.01) than in R1A cell lines (Fig 5B) SAMR1 brain homogenate and Zpl 2-1 cell homogenate

Ngày tải lên: 13/08/2014, 05:21

... lines and tested their effect on cell cycle, apoptosis and Cyclin D1 expression Methods Effect of compounds on RT cell cycle profiles, and cell death were assessed by MTS cell survival assays and ... compounds to induce cell cycle arrest and apoptosis, and to down-modulate Cyclin D1 in RT cells Induction of cell cycle arrest in rhabdoid tumor cells lines treated with 4-HPR and its derivatives ... tested the efficacy of 4-HPR and highly active halogen-substituted (5h and 5j) and peptidomimetic derivatives (11a, 11c, and 11d) on three RT cell lines, MON, G401, and A204 (Figure 1) Since 4-HPR

Ngày tải lên: 13/08/2014, 18:21

... p53-deficient status and all 10 p53 cell lines used (wt+mt), which include the 5 cell lines with wild-type p53-deficient status. Similarly, 117 genes were overlapping between the group of 5 cell lines with ... factor, and hepatocyte growth factor [18,34,37,39-42]. We recently identified specific gene expression signatures in HNSCC cell lines (UM-SCC, University of Michigan Cell Lines Series of Head and ... genes that affect cellular proliferation, apoptosis, angiogenesis, immune and proinflammatory responses, and therapeutic resistance in head and neck squa- mous cell carcinoma (HNSCC) and other cancers

Ngày tải lên: 14/08/2014, 08:20

... CD15 (13%), CD33 (24%) and CD64 (12%) Cell lines The following cell lines were analyzed: K562 (human chronic myeloid leukemia cell line) and HL60 (human acute myeloid leukemia cell line), both obtained ... leukemia cells we ana-lyzed the expression of CD80 and CD86 We determined the expression of these receptors on the cell surface of pri-mary AML cells and leukemic cell lines The pripri-mary cells ... use of primary leukemia cells and leukemia cell lines in cancer gene therapy Background Leukemia cells are obvious and attractive targets for gene transfer since these cells are potentially susceptible

Ngày tải lên: 14/08/2014, 19:22

... other cell types include undifferentiated preadipocytes, immune cells (macrophages and leukocytes), lymph nodes, endothelial and smooth muscle cells, nerve fibers and a matrix of collagen and reticular ... study the molecular and cellular events during adipogenesis The in vitro model includes some adipogenic cell lines (clonal lines 3T3-L1, 3T3-F442A, Ob17, BFC-1, ST13 and A31T) and the primary culture ... assay, cell cycle analysis and apoptosis assay Trang 353 Materials and Methods 3.1 Cell Culture Hep-G2 cell line was purchased from American Type Culture Collection (ATCC) (Manassas, USA) Cells

Ngày tải lên: 03/10/2015, 20:32

... ESR1 and GAPDH are included in Table S2 Cell lines and cultures Five breast carcinoma cell lines were obtained from the Cell Resource Center of Peking Union Medical College (Beijing, China) and ... regulation of cancer cell proliferation and growth For instance, let-7 can inhibit proliferation of lung and liver cancer cells by targeting multiple cell cycle oncogenes [28] and miR-34b and miR-34c ... breast cancer cell lines and tumor specimens (A) For breast cancer cell lines, the expression levels of mature miR-22 were determined by quantitative RT-PCR with U6 as an internal standard miR-22...

Ngày tải lên: 22/03/2014, 21:20

... AML cells are 10-15-fold more resistant to DNR than CD34- AML cells [8] CD34+ KG1a and TF-1 AML cell lines are 30-40 fold more resistant to mitoxantrone than more mature HL-60 and U937 cells, and ... AML cell lines (KG1a and Kasumi-1) and in CD34+ primary AML samples We showed that curcumin selectively induced apoptosis in KG1a and Kasumi1 cell lines, as well as in primary CD34+ AML cells, ... suppression of cell growth of human T -cell leukemia virus type Iinfected T -cell lines and primary adult T -cell leukemia cells Int J Cancer 2006, 118:765-772 21 Kunnumakkara AB, Anand P, Aggarwal...

Ngày tải lên: 18/06/2014, 19:20

... the cell growth of HTLV-1-infected T -cell lines and primary ATL cells Next we examined the effect of AG490 on the growth of HTLV-1-infected T -cell lines and primary ATL cells HTLV1-infected T -cell ... AG490-treated cells Thus, AG490 is both anti-proliferative and pro-apoptotic in HTLV-1infected T -cell lines Figure and primary ATL cells AG490 reduces cell growth of HTLV-1-infected T -cell lines AG490 ... HTLV-1-infected T -cell lines Figure HTLV-1-infected T -cell lines and primary ATL cells AG490 inhibits constitutive activation of Jak and Stat in AG490 inhibits constitutive activation of Jak and Stat...

Ngày tải lên: 13/08/2014, 09:21

... (JNK1/2/3) and p38 isoforms (α, β, γ and δ) Usually, ERK1/2 is involved in cell proliferation and differentiation, while JNK and p38 cascades are activated by cellular stresses However, depending on cell ... p38 siRNA and control siRNA-A were purchased from Santa Cruz Biotechnology 2.2 Cell lines MCF-7, MDA-MB-231 and BT-474 breast cancer cell lines as well as MCF10A normal breast epithelial cell line ... MCF-7 and MDA-MB-231 breast cancer cells (Colin et al., 2010) In addition, the cytotoxicity of 15d-PGJ2 in 786-O, Caki-2 and ACHN human renal cancer cell lines was mediated in caspasedependent and...

Ngày tải lên: 10/09/2015, 09:09