sodium dodecylsulfate polyacrylamide gel electrophoresis and western blotting

... 44 and 46 to 44 kDa band, spot 52 to 39 kDa band, spot 55 and 56 to 37 kDa band, spot 58 to 35 kDa band, spot 59 to 33 kDa band, spot 63 to 28 kDa band, spot 64 to 15.5 kDa band Spot 42, 54 and ... found and considered as corresponding antigen bands on SDSPAGE immunoblotting profiles: Spot corresponds to 80 kDa band, spots 15, 16, 17, 18 and 19 to 65 kDa band, spot 37 to 55 kDa band, spot ... [2] Similar molecular bands, including 17, 29/30, 37 and 46 kDa, were observed from sera of cattle, dogs, sheep, goats, rabbits and pigs [6] Bands such as 15.5-18, 30/31 and 37 kDa, were also...

Ngày tải lên: 07/08/2014, 17:23

... dimension gels SDS-PAGE was performed at constant effect (10 W /gel) for about h and 30 using the Ettan DALT II system (Amersham Biosciences) Staining of gels and gel image analysis Second dimension gels ... 2-DE, gel image analysis, analysis of MALDI results, database search, western blot and drafted the manuscript MA and LOC conceived the study, participated in its design and coordination and helped ... Pteronyssimus and D Farinae), molds (Cladosporium and Alternaria) and animal allergens (cat, dog and horse) SPT were performed on the volar side of the forearm with saline buffer as negative and histamine...

Ngày tải lên: 12/08/2014, 18:22

... labeling and 2D-DIGE analysis KH, AZ and MD performed mass spectrometry data collection and data analysis KH, EP, AZ, EM, MD and AB drafted the manuscript FG carried out the western analysis KH, AB and ... image of the Cy and Cy3 scans from the analytical gel used for additional spot picking and protein identifications The pH and MW range are the same as in Figure 50 μg of pre and post-shock was ... tris-glycine gels (Jules Gels, Milford, CT), sealed with agarose (SDS equilibrium buffer, 0.5% (w/v) agarose, and 0.25% (v/v) of saturated aqueous bromophenol blue) and run at 20 W per gel on the...

Ngày tải lên: 13/08/2014, 13:20

... and role of the PS complex components other than PS We have applied Blue Native gel electrophoresis (BN/PAGE) [19] to analyse further and characterize endogenous native complexes from mouse and ... digitonin before BN/PAGE and Western blotting for PS1 or NCT detection Sharp bands of PS1 reactivity indicated that the 360-kDa PS1 complex contained both PS1 N-terminal reactivity and PS1 C-terminal ... full-length PS1, NTF, CTF and PEN-2 NCT was detected as the mature form APH-1b was detected primarily as a major band of 36 kDa (possible dimer) and as minor bands of 18 kDa and possible trimer at...

Ngày tải lên: 16/03/2014, 16:20

... phospho-affinity gel electrophoresis followed by western blotting with anti-Cdc37 Samples were analyzed after dephosphorylation for (lanes and 7), (lanes and 8), 10 (lanes and 9), and 20 (lanes and 10) ... phospho-affinity gel electrophoresis (A) Non-phosphorylated (lane 1) and phosphorylated (lane 2) Cdc37 were separated by phospho-affinity gel electrophoresis and analyzed by western blotting with ... analyzed by phospho-affinity gel electrophoresis and stained with CBB phospho-affinity gel electrophoresis Phosphorylation was rapid and detected within (Fig 8A, lane 4) and completed within at 30...

Ngày tải lên: 30/03/2014, 03:20

... Fuzzy bands Poor separation of low molecular weight samples Table Advantages and disadvantages of agarose gel electrophoresis 2.2 Polyacrylamide gel electrophoresis (PAGE) Polyacrylamide gels are ... tank and not sink into the gel pocket The gel Gel Electrophoresis – Principles and Basics loading buffer also contains dyes that facilitate observation of the sample during gel loading and electrophoresis, ... analyze the influence of the gel electrophoresis matrix (agarose and polyacrylamide) and staining system (ethidium Introduction to Agarose and Polyacrylamide Gel Electrophoresis Matrices with...

Ngày tải lên: 28/06/2014, 15:20

... storage and optimum resolution in the gels This paper reports the effects of 16 valof electrophoresis buffer pH, tissue storage conditons, and storage times on the capacity of starch gel electrophoresis ... the tissue and type of storage conditions, protocols for enzyme extraction, starch gel preparation and electrophoretic conditions, gel and electrode buffer characteristics, staining and genetic ... experiment, we have investigated the effect of buffer pH (B and H gel gel electrophoresis and electrode buffers) on the resolving power and staining intensities of electromorphs Follwing a conservative...

Ngày tải lên: 08/08/2014, 23:22

... the study and drafted the manuscript GP, LL and MB participated in PFGE and in immunofluorescence studies XS provided informatics and support with statistics for data analysis SM, OC, RM and GC ... system (GelDoc 2000 and Quantity One software, Bio-Rad) The sum of fluorescence within DNA smears was used for calculations and assessing differences in between experiments Determination of H2AX and ... cell-plugs Before electrophoresis, cell-plugs were transferred into ice-cold lysis buffer (pH 7.4) containing 2% sodium lauroyl-sarkosine (Fluka Chemie, Buchs, Switzerland), and 0.5 mg/mL proteinase-K...

Ngày tải lên: 09/08/2014, 09:20

... sugars and sucrose in roots (A, C, E and G) and leaves (B, D, F, and H) and chlorophyll content in leaves (I) of Zea mays plants, previously grown in the absence of nitrogen for 17 days (T0) and ... 2-DE representative gels of the soluble fractions of root and leaf samples are shown in Figure The electrophoretic analyses detected about 1100 and 1300 spots in roots and leaves gels, respectively ... circles and closed squares) and leaves (open triangle and open rhombuses) of Zea mays plants, previously grown for 17 days under nitrogen starvation (T0) and incubated for further 6, 30 and 54...

Ngày tải lên: 12/08/2014, 03:21

... dyes (Cy2, Cy3 and Cy5) and electrophoresis of all the samples on the same 2D gel, reducing spot pattern variability and the number of gels in an experiment and yielding simple and accurate spot ... SDS-PAGE gels were run at 15 mA /gel for 20 and then at 30 mA /gel at 15°C until the bromophenol blue dye front reach the bottom of the gel Scanning and image analysis After 2D-DIGE, scan the gels ... SX, QW and JJ conceived and designed the study SX and QW performed the experiments SX, QW and JJ analyzed data, and wrote the manuscript PC, DM, XY, LQ and YN coordinated the study YC, KZ and XW...

Ngày tải lên: 12/08/2014, 04:20

... variables and Use of western OTC medications and consultations with western medicine practitioners Among all respondents (n = 33,263), 9.4% used western OTC medications only whereas 41.4% used both western ... consultation services and western OTC medications in the previous year 32.7% used western medicine consultation only, and 16.6% used neither western OTC medication nor western medical services ... Table Demographic, socioeconomic and health related characteristics among western medical services and OTC medication users Visited western Used western OTC Consulted western medicine medicine medications...

Ngày tải lên: 25/10/2012, 10:06

... and proteins The centerpiece and "workhorse" of agarose gel electrophoresis is the horizontal gel electrophoresis apparatus There are many types of electrophoresis units, but the horizontal electrophoresis ... Principles and Practice of Agarose Gel Electrophoresis Agarose Gel Preparation Experiment Procedures PREPARING THE GEL FOR ELECTROPHORESIS 11 After the gel is completely solidified, carefully and slowly ... a gel with wells in the middle of the gel After the agarose gel is cast, the gel (on the tray) is placed in the buffer-filled apparatus chamber for sample loading and electrophoresis During electrophoresis, ...

Ngày tải lên: 23/02/2014, 11:10

... between wells and lead to better resolution of the bands.1 • Use both hands when loading the gel; hold the sample-loading device in one hand and use your other hand and arm to support and stabilize ... • Record and evaluate the results of the electrophoresis 10 Clean up • Discard the gel in the trash and pour the salt solution down the drain • Rinse the electrophoresis chamber and gel form ... electrode leads, and remove the chamber lid Remove the gel from the electrophoresis chamber • You may also remove the gel from the form and place it on a piece of plastic wrap • Placing the gel on a...

Ngày tải lên: 13/03/2014, 19:38

... long time reaction and its distribution in particle size is in the broadening range In this article, we report on the preparation of CdSe quantum dots (QDs) by sol -gel method and investigate their ... spectrophotometer Result and discussion To calculate the particle size of QDs we use some following models: + Using absorption spectra to estimate the mean sizes of QDs: The Effros, Brus and Kayanuma’s ... between mean size and specific parameters of QDs [7]: a  a  E g (a ) = E g + π R  B  − 1,786R *  B  − 0,248R * y y  a   a  * y (1) where E g(a) is the effective band gap of QDs with...

Ngày tải lên: 14/03/2014, 13:20

... size, and crystalline phase can be controlled by changing the hydrothermal parameters such as reaction temperature and pressure, pH values, sol composition, type of the solvent and additive, and ... outer diameter and the wall thickness of the titania microtubules being 2–8 and 0.2–2 µm We report in this study a novel anionic template-assisted sol gel approach via urea treatment and under hydrothermal ... sol gel and hydrothermal processing Experimental Titanium tetrachloride (TiCl4 ; 99.9%, Acros) was used as the titanium source; sodium dodecyl sulfate (SDS, CH3 (CH2 )11 OSO3 Na; 99%, Aldrich) and...

Ngày tải lên: 20/03/2014, 13:07

... 4f5/2 and W 4f7/2 are found to lie at 37.1 and 35.20 eV, respectively and are contributed to lattice tungsten in the WO3 crystal 3.2 Gas sensing properties of the thin film sensors Results and discussion ... , CO, and water vapor were measured A given amount of each gas was injected into the chamber and mixed by a fan for 30 s The sensitivity to gases, S, is defined as S = V g /V a , where Vg and Va ... The relationship between the gas sensitivity and TMA concentration at 70◦ C NH3 , gasoline, C2 H5 OH, CH4 and CO at the same concentration of 1000 ppm and to water vapor These experimental results...

Ngày tải lên: 20/03/2014, 13:12

... 2,3,5,7,8-pentamethylchroman 6-sulfonyl (Arg), triphenylmethyl (Cys, Asn and Glu), t-butoxycarbonyl (Lys) and t-butyl (Ser and Tye) Briefly, a standard Fmoc deprotection was used in conjunction with benzotriazol-1-yloxytris(dimethylamino)phosphonium ... °C and were terminated by addition of 1% trifluoroacetic acid (v/v) The internal standard FMRF-amide was added and samples were centrifuged at 20 000 g for 10 at °C Supernatants were collected and ... capillary zonal electrophoresis, several parameters have to be established Fig shows the capillary zonal electrophoresis profile of FMRF-amide (internal standard), angiotensin II, angiotensin I and LORF...

Ngày tải lên: 23/03/2014, 12:20

... 10022-4212 BP Amoco’s key strengths are in oil and gas exploration and production; the refining, marketing and supply of petroleum products; and the manufacturing and marketing of chemicals For the first ... and development In 1995 they increased spending for research and development by 41% and in 1996 research and development expenses increased 67% Total operating expenses were for 1994, 1995, and ... revenues, sales and marketing expenses, general and administrative expenses, and non-operating expenses, which were offset by higher relative research and development expenses and the higher tax...

Ngày tải lên: 23/03/2014, 20:37

... hydrolysis and enzyme function (The prefix L stands for loop, a transmembrane domainconnecting peptide L2/3, L4/5, L6/7, and L8/9 are localized on the cytosolic side, and L1/2, L3/4, L5/6, L7/8, and ... prize lecture ÔAll hands to the sodium pumpÕ J Physiol 462, 1–30 14 Vilsen, B., Andersen, J.P., Petersen, J & Jorgensen, P.L (1987) Occlusion of 22Na+ and 86Rb+ in membrane-bound and soluble protomeric ... interaction with the carbonyl groups of Val304, Ala305, and Ile307 (M4) and through the side-chain oxygen atoms of Asn796 and Asp800 (M6) and Glu309 (M4) [26] A similar situation could be assumed...

Ngày tải lên: 24/03/2014, 00:21

... respectively male and female, yang and yin, sun and moon The anticlockwise version is the Buddhist and Taoist form and was sometimes associated with the Greek goddesses Artemis, Demeter and Hera The ... with the West) and the accompanying exchange of coinage, a rich and varied source of imagery; and the expansion of religion through missions and pilgrimage, especially Buddhism and Christianity ... Shang period representing clouds and rolling thunder and was hence called the 'cloud and thunder pattern' (yun wen and leiwen) The pattern symbolized life-giving rain and the abundance it brought...

Ngày tải lên: 24/03/2014, 09:24