sites implication in dna repair in mammalian cells

... Schar P: DNA Repair in mammalian cells: Mismatched repair: variations on a theme Cell Mol Life Sci 2009, 66:1021-1038 Nouspikel T: DNA repair in mammalian cells: Nucleotide excision repair: variations ... homologous end joining in mammalian cells J Biol Chem 2010, 285:23198-207 58 Lyndaker AM, Alani E: A tale of tails: insights into the coordination of 3’ end processing during homologous recombination ... base pairing exempting pcPNAs from the homopurine target restriction [67] Using N-(aminoethyl)-D-lysine entities the pcPNA backbone can be positively charged resulting in stable DNA duplex invasion...

Ngày tải lên: 10/08/2014, 05:21

... fused to d1-EGFP are maintained in the cells by binding to antigen Generation of soluble binding-defective and cysteine-deleted mutants To demonstrate that the observed intracellular colocalization ... was obtained with scFv 4A-d1-EGFP and 7A-d1-EGFP expressed in parallel Interestingly, in the case of 4A (but not 7A), we found a minor percentage of cells with a faint cytoplasmic staining (arrow) ... the 4A- or the 7A-cotransfected cells, thus demonstrating that strong in vivo binding of the destabilized 13R4-d1-EGFP proteins allows them to be maintained in the cells To rule out the possibility...

Ngày tải lên: 19/02/2014, 18:20

... GAL4 DNA binding domain and VP16 activation domain Various combinations of the EcR ligand binding domain, VP16 activation domain and GAL4 DNA binding domain were constructed (Fig 1A) and tested in ... for binding to ligand as well as for transactivation of reporter genes in cells An EcR gene switch that includes a fusion protein containing VP16 activation domain, GAL4 DNA binding domain and ... EcRtvay) increased ligand sensitivity of EcR gene switch EcR gene switch in a monopartite format containing a fusion protein of VP16 activation domain, GAL4 DNA binding domain and the EcR DEF domains...

Ngày tải lên: 16/03/2014, 12:20

... prevent binding of MCM complex to chromatin [12] In contrast, a recent finding suggests that an intermediate level of phosphorylation of MCM4 is required for chromatin binding during interphase ... growing HeLa cells All seven antiphosphoMCM4 sera recognized the retarded MCM4 prepared from cells in the M phase, indicating that these sites are indeed phosphorylated in the M phase in HeLa cells ... chromatin HeLa cells were separated into Triton-soluble and Triton-insoluble fractions and the insoluble fraction was further separated into DNaseI-soluble and DNaseIinsoluble fractions Proteins in...

Ngày tải lên: 16/03/2014, 13:20

... examined the effects of SA on stress response in mammalian cells using a simple screening system, and revealed that SA is a potent Hsp inducer in mammalian cells, thereby protecting cells against ... HeLa cells were grown in Eagle’s Minimum Essential medium (Nissui Pharmaceutical) containing 10% bovine serum in a CO2 incubator at 37 °C Screening for Hsp modulators A reporter plasmid containing ... into C3H10T1/2 cells (1 · 107 cells per 100 mm dish) with 30 lL lipofectAMINE reagent (Invitrogen) according to the manufacturer’s instructions, and incubated for 48 h Cells were then maintained...

Ngày tải lên: 17/03/2014, 10:20

... to bind to immobilized Mydj2-His protein (lanes 3, 3¢) In contrast, no binding between the immobilized dj2 protein and the inducible hsp70 protein was observed (Fig 6, lanes: 3, 3¢) Interestingly, ... molecular chaperone DnaJ is involved in binding to denatured protein substrates EMBO J 15, 408–417 13 Karzai, A.W & McMacken, R (1996) A bipartite signaling mechanism involved in DnaJ-mediated activation ... the protein seems to accumulate in the nucleus and especially in the nucleolus after heat shock (Fig 4) In order to further investigate the above phenomenon, we proceeded in examining the intracellular...

Ngày tải lên: 17/03/2014, 17:20

... DIFs in mammalian cells [19] Yet, the mechanisms underlying the actions of DIFs in mammalian cells remain to be elucidated Meanwhile, in the course of a study of the in vitro actions of DIFs in mammalian ... that: (a) DIFs increase [Ca2+]c in some tumor cells [9–12], (b) DIFs activate Akt ⁄ protein kinase B in human leukemia K562 cells [13], (c) DIF-1 inactivates STAT3 in gastric cancer cells [15], ... consumption in a way other than by increasing [Ca2+]c or inhibiting PDE1 To support our hypothesis that an increase in [Ca2+]c is not involved in the DIF-1-promoted glucose consumption, we examined...

Ngày tải lên: 23/03/2014, 09:20

... to insulin treatment in vivo are seen in maps generated from eEF1A phosphorylated by MS6K in vitro However, the in vivo maps also contain additional peptides indicating that further insulin-stimulated ... may involve a glycine-rich motif, which would thus serve quite a different function in these kinases from the role of a similar motif in phosphate binding in other protein kinases The eEF2 kinase ... REGULATION OF eEF2 AND eEF2 KINASE BY INSULIN AND OTHER STIMULI Redpath et al [52] showed that, in Chinese hamster ovary (CHO) cells overexpressing the insulin receptor, insulin causes the rapid dephosphorylation...

Ngày tải lên: 23/03/2014, 21:20

... protein have been limited by the fact that its detection in mammalian cells and in the HCV infectious system is elusive This study shows that intracellular levels of the core+1 protein in mammalian ... and 25 lm, respectively The protease inhibitor cocktail for mammalian extracts (containing AEBSF, aprotinin, leupeptin, bestatin, pepstatin a and E-64) was obtained from Sigma (St Louis, MO) Plasmid ... transcribed in the cytoplasm remain uncapped and therefore can be translated only by a cap-independent mechanism In the HCV IRES-containing constructs, initiation of transla- nt 342 ATG initiator in core...

Ngày tải lên: 30/03/2014, 03:20

... of cells in the G0/G1 phase: 78% in control cells versus 75%, 64% and 60% in IBA, RIS and ALE treated cells, respectively Moreover, the same treatment led to an increase in the amount of dead cells ... whose inhibition prevents protein prenylation [2,3] In vitro studies conducted on tumor cell lines suggest that N-BPs are able to exert a broad spectrum of actions, including inhibition of invasion, ... of haploinsufficient and haploproficient strains after the filtering process are given in Additional data files and 5, respectively About 45% of the haploinsufficient strains (including the QD)...

Ngày tải lên: 09/08/2014, 20:20

... trypsin-induced differential protein expression in cervical cancer cells It is interesting and important to know whether trypsineinduced protein alterations in MCF-7 cells are reproducible in other ... MCF-7 cells In contrast to an instantly up-regulated VDAC1 level was shown in MCF-7 cells, a significantly enhanced VDAC1 level in Hela cells starting from hr In summary, trypsinization-induced ... mediating integrin activations in breast cancer cells [17] In addition, one of the most important findings in this study is that trypsinization decreases the growth- and metabolism-related protein expression...

Ngày tải lên: 10/08/2014, 05:21

... competent for autoprocessing in mammalian cells Darunavir and Indinavir inhibit fusion precursor autoprocessing in transfected 293T cells To further examine autoprocessing specificity, we next ... However, it remains to be determined whether TFR regulates protease structures in an auxiliary manner during autoprocessing in the infected cell In line with this, about two decades ago Partin et al ... fusion construct was engineered for mammalian expression to examine protease autoprocessing in transfected mammalian cells Autoprocessing of the fusion precursors carrying protease mutations that...

Ngày tải lên: 10/08/2014, 05:21

... required staining Figure shows that even without staining this imaging method can readily explore in detail the dissimilarities in the internalization of different AuNPs by different cell lines Specifically, ... aggregation of the internalized AuNPs, for 2D and 3D cultures of EMT and HeLa cell (without staining) The distributions were obtained by analyzing EMT cells and HeLa cells These results indicate why ... revealing a situation intermediate between those in EMT and HeLa cells These qualitative conclusions from 2D projection images can be confirmed by examining the specimens from different illumination/imaging...

Ngày tải lên: 11/08/2014, 00:23

... lacking histidine and containing 20 mM 3-AT (figure 1C) In contrast, expression of three mutant http://www.jnrbm.com/content/3/1/5 forms of p53 [1] with point mutations in their DNA- binding domain ... stabilizing action of CP31398 in yeast cells and in human cells CP-31398, a compound isolated in an antibody-based in vitro screen, was reported to stabilize the p53 DNA- binding core domain and ... detected in yeast cells containing the other point mutant variants Average and standard deviation were determined from three independent experiments (C) Growth on selective plates containing 20...

Ngày tải lên: 11/08/2014, 08:20

... secretory cells It was found that while insulin producing β cells of mice are 3.7-5.8µm3, the Golgi stack comprised of 3.1-3.6µm3 [123] Finally, unlinking of the ribbon is an intrinstic G2/M checkpoint ... in the lateral linking between stacks by tethering homotypic cisterna but in a non-redundant fashion, as single depletions resulted in ribbon unlinking [50, 51] While the role 13 of GRASP55 in ... and pancreatic β cells [35, 36] Interestingly, these tubules are only observed in mammalian cells, possibly an advanced feature added to cisternal stacking in mammalian evolution In other organisms...

Ngày tải lên: 10/09/2015, 09:06

... action Inhibit myosin II ATPase activity by binding to myosin II-ADP-Pi Blebbistatin and inhibit the release of the ADP Inhibit actin polymerization by binding to the barb end of the FCytochalasin ... Overexpression an actin crosslinking protein α-actinin increased the accumulation of actin filaments at both equator and the entire cortex, leading to delayed cytokinesis and cytokinesis failure On ... actin FH1 Formin homology FH2 Formin homology F12K Ham's F12 K modification GAP GTPase activating protein GBD GTPase protein binding domain GEF Guanine nucleotide exchange factor GDI the guanine...

Ngày tải lên: 14/09/2015, 14:09

... plasmids were mixed with 6X DNA loading buffer including Sybr-green forming 1X DNA loading solution which was subsequently loaded into the agarose gel The DNA gel was run in Sub-Cell GT System (Bio-Rad, ... representations of the tau protein domains and its phosphorylation sites, indicating the hyperphosphorylated sites (rectangles in red) found in paired helical filaments in AD Tau hyperphosphorylations ... Cdk5 is a serine/threonine kinase and belongs to the cyclin-dependent kinase (Cdk) family (Lew et al., 1995) It was first identified around 1991 and purified from bovine brain in 1995 since which...

Ngày tải lên: 05/10/2015, 22:32

... plasmids were mixed with 6X DNA loading buffer including Sybr-green forming 1X DNA loading solution which was subsequently loaded into the agarose gel The DNA gel was run in Sub-Cell GT System (Bio-Rad, ... representations of the tau protein domains and its phosphorylation sites, indicating the hyperphosphorylated sites (rectangles in red) found in paired helical filaments in AD Tau hyperphosphorylations ... Cdk5 is a serine/threonine kinase and belongs to the cyclin-dependent kinase (Cdk) family (Lew et al., 1995) It was first identified around 1991 and purified from bovine brain in 1995 since which...

Ngày tải lên: 06/10/2015, 20:35

... Prekeris et al 2001) During mitosis, the N-terminal domain of GM130, comprising the p115 binding site, is phosphorylated on serine 25 by CDK1/Cyclin B, thereby inhibiting p115 binding(Nakamura, Lowe ... together in interphase by binding to two Golgins, GM130 and Giantin through its two arms Figure 1.2 COPI vesicle tethering under interphase and mitotic conditions During interphase, p115 dimers link ... organism, the Golgi is inherited as intact, individual stacks into the daughter cells (Nebenfuhr, Frohlick et al 2000) However, in mammalian cells where protein secretion ceases during mitosis, the...

Ngày tải lên: 16/10/2015, 15:35

... Medicine 57 Reagents F12 culture medium (Hangzhou Jino Biology Co., Ltd China), fetal bovine serum (FBS), ampicillin, penicillin and streptomycin (Shanghai Bio-engineering Co., Ltd China), brain ... leading to impairment of the cell cycle in gastric epithelial cells [11] Recent studies suggest Hp can increase the mtDNA mutation in AGS cells and mtDNA mutations have been found in Hp infected ... O2, 85% N2, 10% CO2) with continuous shaking E coli were maintained in the LB liquid medium (containing 100 µg/ml ampicillin) at 37°C for 12 h with continuous shaking Preparation of Hp and E.coli...

Ngày tải lên: 25/10/2012, 11:18