single molecule fluorescence detection mark a osborne

... by a single myosin molecule during interaction with actin Cell 1998, 92:161-171 Harada Y, Funatsu T, Murakami K, Nonoyama Y, Ishihama A, Yanagida T: Single- molecule imaging of RNA polymeraseDNA ... base-pair DNA duplex with an overhanging segment was attached at one end (via a biotin-avidin linkage) to a polystyrene bead 500 nm in diameter and annealed at its distal end to a 15 base-pair ... Lang et al http://jbiol.com/content/2/1/6 References Ishijima A, Kojima H, Funatsu T, Tokunaga M, Higuchi H, Tanaka H, Yanagida T: Simultaneous observation of individual ATPase and mechanical...

Ngày tải lên: 06/08/2014, 18:20

... unfolding rate changes with an applied force, we can obtain estimates for the values of both ku0 and Dxu Given that ku0 = A expACHTUNGRE(ÀDGu/kBT) and assuming a pre-factor, A ~ 1013 sÀ1,[39] we can ... experiments All data was obtained and analyzed using custom software written for use in Igor 5.0 (Wavemetrics, Oswego, OR) There was approximately 0.5 nm of peak-to-peak noise and a feedback response ... 7916–7921 [43] A S R Koti, A P Wiita, L Dougan, E Uggerud, J M Fernandez, J Am Chem Soc 2008, 130, 6479–6487 [44] A P Wiita, S R K Ainavarapu, H H Huang, J M Fernandez, Proc Natl Acad Sci USA 2006,...

Ngày tải lên: 22/03/2014, 18:20

... Ishijima A, Kojima H, Funatsu T, Tokunaga M, Higuchi H, Tanaka H, Yanagida T: Simultaneous observation of individual ATPase and mechanical events by a single myosin molecule during interaction ... interaction with actin Cell 1998, 92:161-171 30 Harada Y, Funatsu T, Murakami K, Nonoyama Y, Ishihama A, Yanagida T: Single- molecule imaging of RNA polymeraseDNA interactions in real time Biophys ... combined single- molecule force and fluorescence measurements can probe biomolecular interactions (a) An optical trap; (b) dual optical traps; (c) an atomic force microscope (AFM) Labeling of a biomolecule,...

Ngày tải lên: 06/08/2014, 18:20

... Kashioka, and S Nakamura 200 9a Recent advances in wfst-based dialog system In Proc INTERSPEECH, pages 268–271 C Hori, K Ohtake, T Misu, H Kashioka, and S Nakamura 2009b Statistical dialog management ... information, and Ω = {A1 , , Aq } is the set of DAs Using the maximum approximation for summation, (1) can be written as A = arg max t A Ω h (A, Ht ) = P r(At = A | At−1 ) W W = arg max P r(W|Ut ... dialogue act detection using named entity classes, partial sentence trees, derivation rules, and entropy-based dialogue actderivation rule matrix is investigated Data-driven dialogue acts are...

Ngày tải lên: 20/02/2014, 05:20

... used in preparation of business plan) 13 Appendix (general background data, research data, additional financial data, marketing materials, etc.) 2013 FIRST NATIONAL BANK BUSINESS PLAN COMPETITION ... Policies • Promotional activities • Media Financial Analysis • Cash flow information • Balance sheet • Projected plan • Narrative about growth Summary Bibliography/Appendix Overall Appearance Conciseness ... visually appealing and complete and accurate information about your business • The business plan may not contain fabricated information about backgrounds, experience, educational level of author,...

Ngày tải lên: 15/03/2014, 20:20

... from a : 50 molar ratio On the other hand, a higher amount of GR was required to observe a similar inhibition as a C1q*/GR molar ratio of : 3000 was at least necessary (data not shown) Such a large ... individual DNA strands This single molecule approach appeared as a valuable tool with which to investigate the binding properties of fucoidan, a sulfated fucose-based polysaccharide known as one ... (fucoidan) and a C1q activator (DNA) are able to bind to the same region of the protein by using not only native C1q but also the C1q isolated domains CLR and the globular region (GR) Materials and...

Ngày tải lên: 16/03/2014, 23:20

... participants were encouraged to investigate alternative approaches in summarization and report their results NeATS participated only in the fully automatic multi-document summarization task A ... Clusters are formed through strict lexical connection For example, Milan and Kucan are grouped as “Milan Kucan” since “Milan Kucan” is a key bigram concept; while Croatia, Yugoslavia, Slovenia, republic, ... D Evans, V Hatzivassiloglou, M Kan, B, Schiffman, -Y and S Teufel 2001 Columbia MultiDocument Summarization: Approach and Evaluation DUC -01 Workshop on Text Summarization New Orleans, LA Radev,...

Ngày tải lên: 17/03/2014, 08:20

... with a minimum target velocity of 0.1 miles per hour (0.147 feet/second) Have a nuisance alarm rate (NAR) and false alarm rate (FAR) not to exceed one per MDARS-E platform per shift Have an image-assessment ... a variety of applications High-power military and commercial radar sensors are typically combined with sophisticated signal processing, making them capable of excellent range accuracy and target ... electronics are based around an Analog Devices ADSP21060 SHARC processor, high-speed analog-to-digital converters to sample the radar signals, and a complex programmable logic device (CPLD) that controls...

Ngày tải lên: 22/03/2014, 11:20

... DNA samples of known genotypes at 50 C SEA/ SEA; SEA/aa; -SEA/aCSa; aa/aa; 5 23.7/aa; 24.2/aa; aQSaa/ aa The peak appeared at 5.1 0.1 min, which indicates aa, aCSa, or aQSa alleles, whereas ... — peak peaks peak — aa/aa CS/aa QS/aa WS/aa of mutation carrier samples, as follows: cases of aCSa/ aa, cases of aQSa/aa, and cases of aWSa/aa (Table I) These results were in accordance with ... /QS cases, and /WS cases) 2a3 .7/aa 2a4 .2/aa 2a3 .7/ -a4 .2 2a3 .7/- 2a4 .2/- 2a3 .7/ -a3 .7 2a4 .2/ -a4 .2 /-aa/aa (including CS/aa cases, QS/aa cases, and WS/aa cases) the original genotype The method has...

Ngày tải lên: 23/03/2014, 22:20

... essentially that of a degenerate decision tree Our object detection framework Feature Evaluation Haar Basis Functions Haar Basis Functions Integral Image Integral Image Haar Basis Functions Haar Basis ... process Stage 11 Stage Stage 22 Stage Ada Boosting Learner Ada Boosting Learner hh 1 Pass Ada Boosting Learner Ada Boosting Learner hh0 hh hh 1 2 Stage 33 Stage Pass 10 Ada Boosting Learner Ada Boosting ... the feature evaluation Speed up the feature evaluation A new image representation  Integral Image A learning algorithm( Based on AdaBoost[5]) Select a small # of visual features from a larger...

Ngày tải lên: 24/04/2014, 13:29

... general, these points may not be available Finally, the need for additional triacs raises physical packaging issue and may not be a cost-effective solution In terms of fault detection in matrix ... fault-indicator J_f ault variable that is updated from the previous interrupt This variable is assigned a value at the end of the fault -detection mechanism that it indicates the location of the single ... Auburn, AL Dr Hung has received several awards for his teaching and research activities, including a Best Paper Award from IEEE T RANSACTIONS ON I NDUSTRIAL E LECTRONICS He served as a Treasurer...

Ngày tải lên: 13/05/2014, 00:55

... and Adam Ray Zachary Najacht prepared virus transport media and coordinated shipments; Diana Goldberg and Richard Zane coordinated the receipt of samples; and Cathy Acker prepared data summaries ... of Alaska, and lower rates discovered along the West coast and Aleutian/Bering Sea Islands (Fig 1) The lowest prevalence rates were found along the Arctic Coastal Plain and in Southeast Alaska ... programs and JCP provided confirmatory virus analysis All authors approved the final version of the manuscript Additional material Additional file Results of avian influenza surveillance in Alaska...

Ngày tải lên: 20/06/2014, 01:20

... prevention, treatment, and care on a global scale Authors and Disclosures Mark A Wainberg, PhD, has disclosed that he has received grants for clinical research from GlaxoSmithKline and Bristol-Myers ... furthering the accessibility and exchange of information among researchers from a diversity of developing-country settings, eJIAS will contribute in a significant way to the advancement of HIV/ AIDS prevention, ... lifetime." Sir Paul Nurse, Cancer Research UK Your research papers will be: available free of charge to the entire biomedical community peer reviewed and published immediately upon acceptance cited...

Ngày tải lên: 20/06/2014, 08:20

... trigram language model described in Section 3.3 and clustered DT adapted acoustic models that are automatically gradually adapted to each individual speaker This unsupervised iterative speaker adaptation ... spontaneous speech data that was built in an effort taken to advance toward the ultimate goals of the MALACH project The novelty of our contribution lies mainly in the fact that we have managed ... audio data available in digital form is growing with an unprecedented pace Actually, we are already trying to address a similar research issue in a joint project with Czech Television (national public...

Ngày tải lên: 20/06/2014, 21:20

... R(nT), C(nT), s TNAR available R, C on sec data TNAR available R, C on sec + prim data No TNAR No TNAR available R, C on sec data TNAR available R, C on sec + prim data No TNAR - 29 - Receivers ... Scholtz [9] from a GLRT approach under a Gaussian noise assumption and assuming the total noise covariance matrix and the useful propagation channel are two unknown parameters The advantages of this ... in satellite location systems or target detection in radar and sonar Among the detectors currently available, a spatio-temporal adaptive detector which uses the sample covariance matrix estimate...

Ngày tải lên: 21/06/2014, 00:20

... Tip-enhanced Raman scattering along a single wall carbon nanotubes bundle Phys Status Solidi B 2010, 247:2818 Hayazawa N, Yano T, Watanabe H, Inouye Y, Kawata S: Detection of an individual single- wall ... nanotubes Anal Bioanal Chem 2009, 394:1787 Yano T, Verma P, Saito Y, Ichimura T, Kawata S: Pressure-assisted tipenhanced Raman imaging at a resolution of a few nanometers Nat Photon 2009, 3:473 Cançado ... single- wall carbon nanotube by tip-enhanced near-field Raman spectroscopy Chem Phys Lett 2003, 376:174 Saito Y, Hayazawa N, Kataura H, Murakami T, Tsukagoshi K, Inouye Y, Kawata S: Polarization measurements...

Ngày tải lên: 21/06/2014, 05:20

... modifications References H Kabata, O Kurosawa, I Arai, M Washizu, S .A Margarson, R.E Glass, N Shimamoto, Science 262, 1561 (1993) Y Harada, T Funatsu, K Murakami, Y Nonoyama, A Ishihama, T Yanagida, ... elasticity as a Magnetic traps Magnetic traps operate similarly to optical traps in that they allow free maneuvering of a bead in solution Just as a particle is trapped in a potential well created ... stretched at pH 8.0 on a poly(methylmethacrylate)-coated surface, measured by a ‘‘photocleavage assay.’’ Each pair of images shows a T7 DNA molecule before (left) and after (right) the photocleavage...

Ngày tải lên: 22/06/2014, 18:20

... total applied bias As a result, the assumption of constant bias is no longer valid This effect can lead to an unchanged apparent barrier height In addition to the tip-sample separation-related barrier ... surface features are recorded and plotted as a function of scan position Each mode has its own advantages The basic constant-current mode was originally employed and can be used to track surfaces ... and molecules directly, based upon structural characteristics and other properties, have become unprecedentedly strong and also practical Characterization of individual atoms and molecules has...

Ngày tải lên: 27/06/2014, 10:20