single cell differential mrna display

... separate cluster, and most of the isolated cell-line cells analysed formed separate clusters A few cells from each cancer cell line were a b Fig 2 Single cell mRNA analysis of pancreatic CTCs a Unsupervised ... all analysed cells b Principal component analysis of the single cell data Each point represents a single cell in the analysis Trang 6markedly different from all the other cell-line cells (Fig.2a); ... Additional file 1: Single-cell Cq values for all cells analysed. (DOCX 36 kb) Additional file 2: Correlation plots of mRNA expression levels in single pancreatic cancer cell-line cells (DOCX 120

Ngày tải lên: 06/08/2020, 07:24

... (TP + TN)/(TP + TN + FP + FN) We generated eight cases: 10 cells, 30 cells, 50 cells, 75 cells, 100 cells, 200 cells, 300 cells, and 400 cells for each condition We noticed that the number of identi-fied ... circulating tumor cells [5] and stem cells Consequently, analyzing gene expression values on the single-cell scale provides deep insight into the interplay between intrinsic cellular processes ... example, single-cell data analysis isimportant in cancer studies, as differential gene expres-sion analysis between different cells can help to uncover driver genes [10] Tools developed for differential

Ngày tải lên: 25/11/2020, 13:14

... Keywords: Single-cell RNA-seq, Time-series data, Dynamic time warping Background Methodological advances provide transcriptomic infor-mation on dozens of individual cells in a single-cell sequencing ... the complex cellular states and to model dynamic biological processes [4] From traditional bulk samples RNA sequencing (RNA-seq) to single-cell RNA sequencing (scRNA-(RNA-seq), cell-to-cell variabilities ... were captured between single cells at four time points The data were generated from RNA from each cell, which was isolated and used to construct a sin-gle mRNA-seq library per cell with a sequencing

Ngày tải lên: 25/11/2020, 17:41

... Introduction to Bioterrorism, Bioaerosol Mass Spectrometry and Single Cell Analysis Introduction Bioterrorism Bioaerosol Mass Spectrometry Single Cell Analysis Trang 12Mass Spectrometer Introduction Proposed ... the FTMS 1s the analyzer cell (ICR cell) Here ions are trapped, excited, and detected The ICR cell is located in the homogeneous region of the magnetic field Many types of cell configurations have ... introduced the first ICR cell in which both ion formation and detection events occur at the same location in the cell.'*° This design differs first in that the trapped ion analyzer cell uses a static

Ngày tải lên: 14/11/2014, 06:13

... known as single-cell protein (SCP) production, referring to the fact that most microorganisms used as producers grow as single or filamentous individuals rather than as complex multicellular organisms, ... of the cell wall The protein content of the cells is approximately two-thirds of the total nitrogen, whereas RNA accounts for 15% and chitin for 10% The chemical composi-tion of single-cell biomass ... low-costfinal product: l Good at breaking down a wide range of complex substrates (e.g., cellulose, hemicellulose, pectin) l Can tolerate low pH values, which helps in preventing contamination

Ngày tải lên: 17/10/2016, 17:34

... diet Thus, single cell protein arises as a perfect administration of United States of America (Gervasi et al., 2018) Alternative to conventional sources for food and feed Single cell proteins ... according to the surveys conducted in recent years Single cell protein (SCP) represents microbial cells grown in mass culture that are harvested as dried cell mass in the form of natural protein concentrate ... for single cell protein production proves to be a cheap and economic substrate and also helps in management of waste The algal protein has been the most highlighted name in the world of single cell

Ngày tải lên: 14/01/2020, 00:33

... specialized cells In plants, successful single cell type analysis has been undertaken for only a handful of cell types, including pollen grains, but also, due to ease of isolation, cells of the ... Conclusions: This single-cell type omics approach shows that epidermal bladder cells of M crystallinum are metabolically active modified trichomes, with primary metabolism supporting cell growth, ... Trang 2Single-cell-type analysis is a powerful experimental ap-proach, allowing for the capture of information from specific cell types that would normally be lost due to the heterogeneity of cells

Ngày tải lên: 22/05/2020, 04:26

... These cells types are: HNSCC Malignant cells, Fibroblasts, Macrophages, Dendritic cells, Endothelial cells, Mast cells, B cells, conventional CD4+T cells, T-regulatory cells, conventional CD8+ T cells, ... stromal cell types including Fibroblasts, B cell, Macrophages, Endothelial cells, Dendritic cells, Mast cells and T cells (Additional file1: Figure S2A and Additional file1: Figure S2B) (3) T cell ... Identifiable cell types using HNSCC single cell data Overall, the adaptive clustering analysis on single-cell transcriptome data pooled from all HNSCC tumor sam-ples identified distinct 11 cell clusters

Ngày tải lên: 17/06/2020, 16:53

... patient 2 Trang 6total, 40 single cells of patient 1 (ten single cells for eachregion) and 48 single cells of patient 2 (eight single cells for region D and ten single cells for the other regions) ... performed single-cell WGS to access the ITH of each region at the single-cell level Tumor cells were Trang 5sorted by FACS based on the 7-AAD−Lineage−EpCAMhighbiomarker combination [36] and then single ... collected and stored at−20 °C The fluorescent activated cell sorting (FACS) and single-cell isolation To isolate single tumor cells, cryopreserved cells were thawed and stained with combinations of

Ngày tải lên: 06/08/2020, 03:14

... reference for each single lymphoblast cell WGA product was a pool of WGA DNA from multiple (5–10 single cell) WGA reactions from either male or fe-male lymphoblast reference cell lines Gender-mismatched ... three experiments in which a single cell was spiked in, that one cell was identified In one sample that had 3 mCTCs spiked in, 5 cells were identified Upon review of the cells by a board-certified ... of single cells from slides was performed with CytePicker that is integrated with CyteFinder (Additional file 5: Figure S5) CytePicker is a hydraulically controlled semi-automated single cell

Ngày tải lên: 30/09/2020, 10:50

... prognostic implications of ASCs Single cell sorting for CD34+CD38- cells and CD34+CD38+ cells The proportion and frequency of ASC were examined using a single cell sorter (BD FACS Aria, BD Biosciences, ... and designated AML cell populations The mtDNA copy numbers were analyzed for the col-lected bulk cells from the CD34+CD38- cells (ASCs), CD34+CD38+ cells, CD33+ cells, and CD19+ cells Total DNA ... stem cell signaling profiler array, we found that the expression of 27 genes Figure 1 Morphology of single hematopoietic and AML stem cell clones Single cells, either hematopoietic stem cells

Ngày tải lên: 30/09/2020, 12:34

... replicate samples of single MCF7 cells (positive control), single DTCs, and single CTCs, but was not detected in single WBCs (wild type/ negative control) or single BT474 cells (which carry a ... two MCF7 single cells shown here carry the G1633A heterozygous mutation; Normal DNA, BT474 single cells, and single WBCs are wild type (G/G); PIK3CA G1633A mutations were detected in single DTCs ... DAPI+, as expected of tumor cells (Figure 2B and C). PIK3CA gene mutations To first assess our mutation detection method, targeted DNA from single tumor cells or cells from cell lines was successfully

Ngày tải lên: 14/10/2020, 17:23

... patient TNBC of [34] The input data consists of single-nucleus exome sequencing of 32 cells: 8 aneuploid (A) cells, 8 hypodiploid (H) cells and 16 normal cells (N) (Fig 6a) Wang et al considered clonal ... we can read geno-types of individual cells via single-cell sequencing (SCS) Despite this theoretical advantage, however, current tech-nical challenges in cell isolation and genome amplification ... accumulation of mutations in single cells under the ISA As bulk samples usually include intermixed tumour sub-populations, we simulated bulk datasets by pooling single-cell genotypes generated as

Ngày tải lên: 25/11/2020, 12:15

... likely of Macrophages/Monocyte cells Figure 1d shows an example of another important marker, CD38, expressed in many immune cells including T cells, B cells and Monocyte cells We would like to emphasize ... Background: Single cell RNA sequencing (scRNA-seq) brings unprecedented opportunities for mapping the heterogeneity of complex cellular environments such as bone marrow, and provides insight into many cellular ... DigitalCellSorter Conclusions: This methodology assures that extensive marker to cell type matching information is taken into account in a systematic way when assigning cell clusters to cell types

Ngày tải lên: 25/11/2020, 12:42

... able to predict network structures from single cellexpression data accurately, even if they are specifically developed for single cell methods Also, single cell methods, whichusually depend on more ... and CLR, and three single cell-specific methods: SCENIC, SCODE and PIDC – were applied to two single cell experimental datasets, and two simulated datasets that resemble single cell data Evaluation ... specific aspects of analyzing single cell gene expression data MAST [4] assesses differential gene expression whileaccounting for technical variation in single cell data,whereas scDD tests for

Ngày tải lên: 25/11/2020, 13:57

... the cells is necessary for the maintenance of oscillations Removal of external Ca2+ leads to a cessation of oscillations in most cases in endodermal cells [62] and HeLa cells [63] In other cell ... However, cellular Ca2+ transients also have a spatial dimension In the cytoplasm of single cells, Ca2+ gradients can be observed when Ca2+ release from the ER is excited at particular subcellular ... that the cells oscillating faster in isolation peak before the slower cells Thus the intercellular coupling leads to a (near) : entrainment, or synchrony, of the oscillations in adjacent cells On...

Ngày tải lên: 24/03/2014, 00:21

... average gene expression of a culture of isogenic cells and the gene expression of a single cell within such a culture [15,16] Therefore, single cell analysis with high spatiotemporal resolution ... processes within a cell Single cell analysis is not yet widely applied mainly because of the fact that the technologies that are needed are not fully developed The fact that microbial cells are much ... mRNAs per cell [22] Assuming that these mRNAs comprise 5% of the total RNA [20,23] and that the average RNA length is 2,500 nucleotides [20], S cerevisiae would contain 2.5 pg total RNA per cell...

Ngày tải lên: 09/08/2014, 23:20

... detection of single EBV-transformed cells using the GADA algorithm Single- cell CNV detection of all seven EBV-transformed cells Each row represents the profile of one EBV-transformed cell and each ... contributions JRV designed the novel single- cell experiment and critically reviewed the manuscript EV and TV designed the novel single- cell experiment, carried out the single- cell experiments and generated ... number variation detection of single EBV-transformed cell 1168 using existing Page 12 of 14 normalization methods (a-d) Single- cell CNV detection of EBVtransformed cell 1168 after global loess...

Ngày tải lên: 09/08/2014, 23:20

... continuous light The cells used for on-chip cultivation were taken from the culture in the early-log phase (≈104 cells/ml) On-chip single- cell cultivation system The on-chip single- cell cultivation ... the absolute cell volume, whereas ours is based on the relative cell volume (a ratio of the current cell volume to the initial cell volume) Discussion To investigate cell growth and cell cycle ... dynamics of single- cell gene expression over multiple cell cycles Mol Syst Biol 2005, 1, 2005.0024 13 Wakamoto Y, Yasuda K: Quantitative evaluation of cell- to -cell communication effects in cell group...

Ngày tải lên: 11/08/2014, 00:22

... sequence up to 25 single cells on a single- flow cell lane, thus allowing 200 single cells to be profiled in a single run Moreover, by decreasing the genomic resolution of each single- cell copy number ... of cells present, but further to obtain genetic information from the rare cells that may be the most malignant Isolating single cells To study a single cell it must first be isolated from cell ... Methods for single- cell genomic profiling Several methods have been developed to measure genome-wide information of single cells, including cytological approaches, aCGH and single- cell sequencing...

Ngày tải lên: 11/08/2014, 12:21