single cell cultivation chip system

... light The cells used for on-chip cultivation were taken from the culture in the early-log phase (≈104 cells/ml) On-chip single-cell cultivation system The on-chip single-cell cultivation system ... their regulation in the eukaryotic cell cycle, we used an on-chip single-cell cultivation system and Chlamydomonas cells as the model system The Chlamydomonas cell cycle was divided into interdivision ... mechanism of cell cycle regulation in the unicellular green algae Chlamydomonas reinhardtii, we developed an on-chip single-cell cultivation system that allows for the strict control of the extracellular...

Ngày tải lên: 11/08/2014, 00:22

... subsequent single-cell sequencing or single-cell cultivation experiments During the whole procedure from pumping to single-cell droplet isolation, cell suspension and oil were pumped into the chip ... limit of single-cell droplets (λ = 1, black circle) (c) Result of the single-cell cultivation experiment S: single-cell samples; N: blank droplet (d) Microscopic images of P rhodozyma cells from ... a single cell, thus the present single-cell isolation method can be used for analysis of gene expression heterogeneity among microbial populations Single-cell whole genome sequencing Single-cell...

Ngày tải lên: 24/11/2022, 17:54

... the cells is necessary forthe maintenance of oscillations Removal of external Ca2+leads to a cessation of oscillations in most cases inendodermal cells [62] and HeLa cells [63] In other celltypes, ... along time in periodically contracting muscle cells (e.g heartcells) and neurons [1], before they were discovered in themid-1980s in nonexcitable cells, notably in oocytes uponfertilization [2] ... hormonestimulation [3,4] Later, they have also been found in manyother animal cells (cf [5–10]) as well as in plant cells [11],with many of these cells not having an obvious oscillatorybiological function The...

Ngày tải lên: 24/03/2014, 00:21

... plant cells in more detail, we established a cell-free protein N-myristoylation system as an application of an advanced wheat germ cell-free translation system that allows protein synthesis on a ... in the wheat germ cell-free translation system Effect of the combination of amino acids at positions 3 and 6 on protein N-myristoylation in the wheat germ cell-free translation system Ser6 in the ... germ cell-free translation system Wheat germ extracts have previously been shown to contain protein N-myristoylation activity [25] To inves-tigate the mode of protein N-myristoylation in plant cells...

Ngày tải lên: 29/03/2014, 21:20

... average gene expression of a culture of isogenic cells and the gene expression of a single cell within such a culture [15,16] Therefore, single cell analysis with high spatio-temporal resolution ... AccessSingle cell transcriptomics of neighboring hyphae of Aspergillus niger Charissa de Bekker1, Oskar Bruning2, Martijs J Jonker2,3, Timo M Breit2,3and Han AB Wösten1* Abstract Single cell profiling ... processes within a cell Single cell analysis is not yet widely applied mainly because of the fact that the technologies that are needed are not fully developed The fact that microbial cells are much...

Ngày tải lên: 09/08/2014, 23:20

... Detection of chromosomal aberrations from a single cell by array comparative genomic hybridization (single-cell array CGH), instead of from a population of cells, is an emerging technique However, ... plot of a single EBV-transformed cell (a-c) MA plot for EBV-transformed single lymphoblastoid cell 1162 before normalization (a), genomic DNA before normalization (b), EBV-transformed single lymphoblastoid ... one model It would be attractive to enrich this model by accounting for single-cell data properties for single-single-cell CNV detection in the near future Application 2: human embryo array CGH...

Ngày tải lên: 09/08/2014, 23:20

... sequence up to 25 single cells on a single-flow cell lane, thus allowing 200 single cells to be profiled in a single run Moreover, by decreasing the genomic resolution of each single-cell copy number ... individual locus in a single cell, it could not amplify the entire Figure 2 Isolating single cells and techniques for genomic profiling (a-c) Single-cell isolation methods (d-f) Single-cell genomic profiling ... of cells present, but further to obtain genetic information from the rare cells that may be the most malignant Isolating single cells To study a single cell it must first be isolated from cell...

Ngày tải lên: 11/08/2014, 12:21

... the cell cycle are single-cell studies, where growth during the cell cycle of a single cell is measured, and cell-culture studies, where growth during the cell cycle of a large number of cells ... that single cell studies aremore misleading than population studies Understanding cell growth should be based on cell culture behavior rather than single cell studies It is also argued that single-cell ... Mitchison has proposed that single-cell studies, because they show variations in cell growth patterns, are more suitable for understanding cell growth during the cell cycle, and should be preferred...

Ngày tải lên: 13/08/2014, 23:20

... Introduction to Bioterrorism, Bioaerosol Mass Spectrometry and Single Cell Analysis Introduction Bioterrorism Bioaerosol Mass Spectrometry Single Cell Analysis Trang 12Mass Spectrometer Introduction Proposed ... the FTMS 1s the analyzer cell (ICR cell) Here ions are trapped, excited, and detected The ICR cell is located in the homogeneous region of the magnetic field Many types of cell configurations have ... 28cell relies entirely on a pulsed mode of operation while the drift cell configuration used a continuous current of ionizing electrons and continuous drift of ions through the length of the cell...

Ngày tải lên: 14/11/2014, 06:13

... 4] There are two types of stem cells - adult stem cells and ES cells Adult stem cells are found within adult organs whereas ES cells are derived from the inner cell mass of pre-implantation embryos ... Embryonic Stem Cells Stem cells are cells that are at an early stage of development, which can proliferate by self-renewal and retain the potential to differentiate into two or more cell types [1, ... wells were sacrificed for cell counting at each passage to obtain the number of cells and from there calculate the population doublings The wells were harvested into single cell suspensions using...

Ngày tải lên: 11/09/2015, 10:06

... it is a sign of the aging of the cells hESCs on feeder-free culture systems tend to acquire senescence at earlier passages compared to those on feeder co-culture systems, and senescence is usually ... ratios, resembling spontaneously differentiated cells Although hESCs on DxSDOCDOC did have some spontaneously differentiated cells in the population, these cells were not common Colonies of hESCs on ... matrices For each hESC sample, triplicates of 10,000 labeled cells were used for each marker An average of the percentage of positively-labeled cells of each triplicates was plotted, together with the...

Ngày tải lên: 11/09/2015, 10:07

... differentiation using a protocol modified from WiCell Research Institute, National Stem Cell Bank In this protocol, the hESCs formed embryoid bodies using non-adherent cell culture plates and were transferred ... regulating cellular differentiation, and because little is known about the epigenetic factors that define the differentiation state or potency of stem cells in comparison to differentiated cells, ... colonies consist of cells with high nuclear to cytoplasm ratio In contrast, hESC cultured on Matrigel grew in larger colonies, albeit still with defined colony edges These control cells still retained...

Ngày tải lên: 11/09/2015, 10:07

... Oct-4 is an intracellular pluripotency marker, which requires extra processing of the cell suspension to permeate the cellular membrane and nuclear membrane, thus resulting in more cells lost in ... sizes of colony cells compared to the differentiated cells hESCs on DxSDOC grew in large round colonies that still had a dense morphology The colonies have distinct borders and the cells within ... the limitations of using an intracellular pluripotency marker (Oct-4) the other pluripotency markers included were cell surface markers, hence preventing excessive cells lost to processing steps...

Ngày tải lên: 11/09/2015, 10:07

... molecules into a host cell through its membrane by giving quick electrical pulses across the cell For this thesis, the design and fabrication of a novel single cell electroporation biochip by the proton ... images of these stained electroporated cells on our biochips further proves that the stain has been successfully bound to the DNA This single cell electroporation system is a promising method for the ... and cell passage protocols The cell recovery is the method for recover the cell from being kept alive at very low temperature (-150 o C) The freeze cells were immediately added into the fresh cell...

Ngày tải lên: 11/09/2015, 10:16

... other macrophages [89,108,76,109], nerve cells [110] and other cells[111] are available This is an expected phenomenon for phagocytic cells (macrophages) and cells that function as a barrier and/or ... endothelial cells J Cell Sci 1988, 91:231-238 Morgan DM, Larvin VL, Pearson JD: Biochemical characterisation of polycation-induced cytotoxicity to human vascular endothelial cells J Cell Sci 1989, ... association with the cell membranes after application to human skin [91] The authors suggested that single molecules, which make up the particles, may penetrate the intercellular spaces and,...

Ngày tải lên: 11/08/2014, 00:22

... [27] Cultures (5·104 cells/well) of P-ECs and of PA-SMCs were prepared, and IL-6 levels in the culture cell lysates were measured using an ELISA (R&D Systems, Lille, France) Cells were used for ... receptors are expressed not only by inflammatory cells, but also by constitutive vesselwall cells Thus, both pulmonary vessel cells and inflammatory cells may be targets for IL-6 Since the hypoxic ... performed in duplicate using the ABI Prism 7000 system (Applied Biosystems Foster City, CA) PCR primers were designed using Primer Express Software (Applied Biosystems) To avoid inappropriate amplification...

Ngày tải lên: 12/08/2014, 14:20

... of TLRs in lung cells or tissue TLRs are widely expressed on both resident lung cells as well as infiltrating cells of myeloid and lymphoid origin Primary bronchial epithelial cells express mRNA ... mRNA [35] Finally, lung tissue cells may also be activated through cooperative interactions with TLR responsive lymphoid cells, as exemplified by airway smooth muscle cell activation via IL-1b release ... IL-10 [184] while basophils may also instruct T cells to become Th2 cells [185] TLRs have been shown via genetic association studies as well as single and multiple gene knockout studies to play...

Ngày tải lên: 11/08/2014, 03:20

... differential cell counts of the bronchoalveolar lavage fluid (BALF) The total cell count was measured by using a hemocytometer The differential cell count was determined by manually counting 200 cells ... fibroblast proliferation and synthesis of extracellular matrix, occur [9] Various types of cells, including macrophages and neutrophils have been the immune cells primarily implicated as playing potential ... cells and neutrophils were barely detected as infiltrating cells in the lung (data not shown) Discussion The ability of CD69 to function as a signal transducing molecule in various types of cells,...

Ngày tải lên: 12/08/2014, 14:20

... location of ED-1 positive cells was determined with immuno-electron microscopy, we counted these cells in lung sections under a light microscope BDL rats showed more ED-1 positive cells in their alveolar ... Quantification of ED-1 positive cells The methods have been described previously [15] An observer, who was blinded to the identity of treatment groups, counted the ED-1 positive cells in alveolar septa ... sections from control rats (C) contained occasional septal ED1-positive cells (arrow) while those from BDL rats had numerous reactive cells in the septa (arrows) Immunoelectron micrograph (E) shows gold...

Ngày tải lên: 12/08/2014, 14:20

... signal intensity under different experimental conditions (d) shows a single bronchiole from a non-sensitized mouse, (e) A single bronchiole from a sensitized mouse treated with control mismatched ... inflammation (Figure 3e) with goblet cell hyperplasia, compared with the non-sensitized animals No differences were found in the total number of inflammatory cells or in eosinophil accumulation ... since in vitro and ex vivo data suggest that this PG contributes to migration of mast cells and dendritic cells and, therefore, promotes inflammation [49,50] However, we believe that a more complex...

Ngày tải lên: 12/08/2014, 14:20