single cell and single molecule analyses using microfluidic devices

... variety (Z141) and drought-sensitive variety (NY-17) under DS and RD stress at the seedling stage using single-molecule real-time sequencing and RNA-sequencing Gene Ontology (GO) and reduce and visualize ... drought stress (left) and controls (right) g, h Z141 and NY-17 ALWC and RLWC with means and SEs (n = 3) respectively The abscissa indicates ASWC, and the ordinates indicate ALWC (g) and RLWC (h) Blue ... study, 2275 and 1343 genes were upregulated, and 3067 and 2154 were downregulated when Z141 and NY-17 were under DS, respectively In total, 1007 and 1686 genes were significantly up- and downregulated

Ngày tải lên: 24/02/2023, 08:23

... Mn4+Mn3+3 molecules. Trang 10valance manganese ions and ligands in the molecule In thenext section, we will explore spin polarizations on ligands, as well as exchange couplings between Mn and ligands, ... K, and –63.28 K, and JBB/kB= 23.32 K, 24.78 K, and 24.33 K for (14), (15), and (17) respectively These values inferred from previous experiments are JAB/kB= –33.24 K, –29.93 K, and –40.86 K, and ... ligands When both L1 and L2 are neutral ligands, the modeled molecules are expected to be Mn4+Mn2+3O3Cl(RCOO)3(L,L)3 These are denoted as Mn4+Mn2+3 or Mn4(L,L)3 When L1 and L2 are a neutral ligand

Ngày tải lên: 16/12/2017, 17:21

... variation References Anandakumar, C.R and Ganesamurthy, K., 2015 Single Marker Analysis for Oil Yield and Component Traits in Journal, 102(1-3), pp.6-9 Bhat, R S., Patil, V U., Chandrashekar, T M., ... undertaken to evaluate and characterize mutants for associating productivity, disease resistant and quality traits using transposon specific (AhMITE1) markers Trang 3Materials and Methods Field experiment ... replications Standard package of practice was followed to raise the crop Mutants and their parents were evaluated for growth, morphological, productivity and nutritional traits, and response to

Ngày tải lên: 14/01/2020, 00:13

... were quantified using the Qubit assay and library size was confirmed using the bioanalyser 12000 kit Following SMRTbell™ construc-tion, v2 primers and P4 polymerase were annealed and en-zyme bound ... amplicon library was loaded on to one SMRT cell and sequenced on the PacBio RS II in-strument using C2 chemistry and a 120 minute movie time PacBio data analysis and mutation detection Detection of ... 10%, 1% and 0.5% Library preparation and PacBio sequencing Long range PCR amplification of the BCR-ABL1 p210 transcript was performed using the Clontech Advantage PCR kit (Clontech, CA, USA) UsingBCR-ABL1

Ngày tải lên: 30/09/2020, 13:43

... patient TNBC of [34] The input data consists of single-nucleus exome sequencing of 32 cells: 8 aneuploid (A) cells, 8 hypodiploid (H) cells and 16 normal cells (N) (Fig 6a) Wang et al considered clonal ... reliable models of single-tumour evolution, quantify the extent of intra-tumour heterogeneity and generate new testable experimental hypotheses Keywords: Single-tumour evolution, Single-cell sequencing, ... in single cells under the ISA As bulk samples usually include intermixed tumour sub-populations, we simulated bulk datasets by pooling single-cell genotypes generated as described above, and

Ngày tải lên: 25/11/2020, 12:15

... labelling All D+ cells showed a fluorescence signal In total, we analysed 5894 cells using Atto655-H41-Ab (average cell count per sample 115), 5826 cells using Atto655-BRAD3-Ab (average cell count ... classification: 58% of all D+ and 53% of all D− samples were classified correctly using Atto655-Brad3-Ab labelling, 75% and 84% using Atto655-BIRMA-D6-Ab labelling, and 83% and 76% using Atto655-H41-Ab ... Fig. 5 Trang 9Data analysis – cell and single molecule detection All data analysis tasks were performed using implemented and adapted image processing techniques For cell detection tasks we applied

Ngày tải lên: 04/12/2022, 16:21

... The whole composite bridge (leading to cell nucleuses at two ends) for cell-cell adhesion Basic Materials: (F-Actin, -Catenin) for Cell 1 and Cell 2, and other molecular complexes (two blue ... Microscopy (AFM) and Magnetic Tweezersb, with an emphasis on AFM The background on cell-cell adhesion and cell sensing-response will be given and defined An interesting molecular model of cell sensing-response ... of the single-molecule experimental techniques used 2.1) Scientific Background: 2.1.1) Cell adhesion – cell sensing response Biological cell-cell adhesion means the sticking of two cells which

Ngày tải lên: 01/10/2015, 17:27

... calculators This can also befound using appropriate commands on other calculators, using a computer, or using aprobability table for the standard normal distribution Press STAT and arrow over to TESTS ... 83+, and 84+ calculators (Solution B) Solution ATo find the confidence interval, you need the sample mean,¯x, and the EBM. Trang 62 = z0.05= 1.645 using invNorm(0.95, 0, 1) on the TI-83,83+, and ... normally distributed with an unknownpopulation mean and a population standard deviation of three points A random sample of 36 scores is taken and gives a sample mean (sample mean score) of 68 Find

Ngày tải lên: 19/10/2016, 22:06

... Trang 1A Single Population Meanusing the Student t sample standard deviation s as an estimate for σ and proceeded as before to calculate a confidence ... σ. If you draw a simple random sample of size n from a population that has an approximately a normal distribution with mean μ and unknown population standard deviation σ and calculate the t-score ... mean,¯x, and the EBM. Trang 5We estimate with 95% confidence that the true population mean sensory rate is between7.30 and 9.15.Press STAT and arrow over to TESTS Arrow down to 8:TInterval and press

Ngày tải lên: 31/10/2017, 17:24

... find primary landmarks such as the head, hands, and feet A 3D human pose was reconstructed by fitting a human skeleton model to the landmarks In [2], using the information of primary landmarks as ... arms, left and right upper and lower legs) and 9 joints (two knees, two hips, two elbows, two shoulders, and one neck) There were a total of 24 DOFs (including two DOFs at each joint and six free ... cloud P musing a logistic function and the vec-tor of determined Mahalanobis distances, as in (5) Step 4. Compute the PDA mean vector μ∗ and PDA covariance matrix S∗ of the point cloud P m using

Ngày tải lên: 12/12/2017, 06:32

... 10.1142/S0219876213400082FREE AND FORCED VIBRATION ANALYSIS USING THE n-SIDED POLYGONAL CELL-BASED SMOOTHED FINITE T NGUYEN-THOI∗,†,,∗∗, P PHUNG-VAN†, T RABCZUK§, H NGUYEN-XUAN∗,†and C LE-VAN¶ ∗ Department ... meshfree methods [Liu and Nguyen Thoi (2010); Liu et al (2010); Rabczuk et al. (2004); Rabczuk and Belytschko (2005)], while the CS-FEM model is closer to the standard FEM and simpler than other ... coefficient and∇ su is the symmetric gradient of the displacement field [Bathe et al (1996); Liu and Quek (2003); Hughes (1987); Zienkiewicz and Taylor (2000)] The FEM uses the following trial and test

Ngày tải lên: 16/12/2017, 00:04

... in the core μ3-Cl ligand and the O2CMe ligands, the local electronic structures at Mn sites are also changed Therefore, combining variations in μ3-O, μ3-Cl, and O2CMe ligands is expected to be ... fields at A and B sites are (11) the distance between the A site and Z1 (d AZ1), (12) the distance between the B site and O xy (d BO xy), and (13) the distance between the B site and O z (d BO ... (by data mining using electronic features and substitutional structural features of ligands) and calculated (by DFT) exchange couplings J AB /k Bfor the 114 (blue solid circles) and the newly designed

Ngày tải lên: 16/12/2017, 10:33

... single-molecule fluorescence polarization J,N Forkey, M.E, Quinlan and Y.E Goldman 1 Single molecule force spectroscopy in biology using the atomic force microscope / Zlatanova, SM Lindsay and ... Twisting and stretching single DNA molecules T Stricky J.-F Allemand, V Croquette and D Bensimon 115 Part II (reprinted from Progress in Biophysics & Molecular Biology 77/1) Single molecule ... by single-molecule force spectro-scopy and protein engineering M Carrion-VasqueZy A.F Oberhausen T.E Fisher, PE, Marszalek, H Li and J.M, Fernandez 63 The importance of molecular structure and

Ngày tải lên: 10/05/2019, 13:43

... single-molecule fluorescence polarization J,N Forkey, M.E, Quinlan and Y.E Goldman 1 Single molecule force spectroscopy in biology using the atomic force microscope / Zlatanova, SM Lindsay and ... Twisting and stretching single DNA molecules T Stricky J.-F Allemand, V Croquette and D Bensimon 115 Part II (reprinted from Progress in Biophysics & Molecular Biology 77/1) Single molecule ... by single-molecule force spectro-scopy and protein engineering M Carrion-VasqueZy A.F Oberhausen T.E Fisher, PE, Marszalek, H Li and J.M, Fernandez 63 The importance of molecular structure and

Ngày tải lên: 14/05/2019, 11:37

... methods for static and free vibration analyses of trapezoidally and sinusoidally corrugated panels Using the results of ANSYS and ABAQUS shell simulations as references, the accuracy and reliability ... (Fig 4-1a and Fig 4-1b) and original corrugated panels are simulated using constructive rectangular meshes (Fig 4-1c and Fig 4-1d) The element types used in ANSYS and ABAQUS are SHELL181 and S4R, ... solved using the cell-based smoothed MIN3 (CS-MIN3) 3.1 Galerkin weak form for static and free vibration analyses of equivalent Reissner-Mindlin orthotropic plate The displacements in x -, y -, and

Ngày tải lên: 28/01/2021, 19:11

... Preparation of samples for single molecule fluorescence 5 Fluorescence spectroscopy of freely diffusing single molecules: examples 201 5.2 Single molecule studies of freely diffusing molecules 201 6 Fluorescence ... introduction of the two coregroups of single molecule fluorescence experiments: measurements of diffusingsingle molecules and measurements of immobilized single fluorescent molecules,in order to provide ... et al., Single molecule detection using surface-enhanced Raman scattering (SERS) Physical Review Letters 78 (1997) 1667–1670. [20] Nie, SM and Emery, SR, Probing single molecules and single nanoparticles

Ngày tải lên: 10/05/2022, 14:54

... 293T/CD20 cell line, but not to the control 293T cell line In contrast, the lentiviral vector FUW/VSVG was able to bind to both 293T and 293T/ CD20 cell lines In addition, a quantitative virus -cell ... internalization of the viral particle Cells were then stained for the presence of viral particles on the cell surface using an anti-FM antibody and quantified using flow cytometry As shown in Fig ... transfected cells Solid line, analysis on transfected 293T/CD20 cells; shaded area, analysis on 293T cells (B) Analysis of co-expression of the FM and antibody on gated GFP-positive cells FM was...

Ngày tải lên: 12/08/2014, 04:21

... histone-fold extensions of H3 and H2B are labeled as αN and αC, respectively; the interface between the H2A docking domain and the H4 C terminus as b; and iv N- and C- terminal tail regions as ... H4; histone-fold extensions of H3 and H2B are labeled as αN and αC, respectively; the interface between the H2A docking domain and the H4 C terminus as b; and N- and Cterminal tail regions as N ... support and encouragement when I was in his group for the past years Without these, I would not have made so many achievements and it is an interesting and enriching experience for doing research and...

Ngày tải lên: 14/09/2015, 08:39

... spectrofluorimeter using a · cm pathlength cuvette, using the same conditions as in the CD experiments, with excitation at 280 nm and a bandpass of nm, and emission at 320 nm with a bandpass of nm ... spectrum (Fig 2B and Table 1) Functional analyses The functional characteristics of the chimeras were analysed by cosedimentation with actin and tropomyosin followed by SDS ⁄ PAGE, and by their ability ... and TnI regulatory domains Fig (A) TnC(1–91)–TnI(98–182) experimental data and GNOM fitting Inset: pair distance distribution function p(r) The experimental values ˚ ˚ for Dmax and Rg, 110 A and...

Ngày tải lên: 16/03/2014, 18:20

... the rupture of A’ and G b-strands can be facilitated by the breaking of interstrand hydrogen bonds by D2O molecules These molecules form bridges between the two separating strands (Figure 5) One ... Snapshot of the bstrands A’ and G of the I27 protein showing D2O molecules bridging the protein backbone Steered molecular dynamics simulations measure the elongation of b-strands A’ and G for unfolding ... biological function, where they play a key role in cell adhesion and motility, formation and stability of proteins structures and receptor– ligand interactions.[3] To further explore the role of...

Ngày tải lên: 22/03/2014, 18:20