signaling on reprogramming and stemness transition in somatic cells

... differential effects on cancer cells in culture Garcinol treatment alters expression of chromatin modifying enzymes in MCF7 cells, resulting in reprogramming of key histone and p53 PTMs and growth arrest, ... resulted in a dose-dependent reduction in the proportion of cells entering S phase, as indicated in Figure 1C In contrast to garcinol compounds, curcumin-treated MCF7 cells showed an increase of cells ... func-tion is regulated by lysine acetylafunc-tion, which impacts on its function in transcription, DNA damage checkpoints and cell fate decisions Acetylation of lysines in the C-terminal activation

Ngày tải lên: 05/11/2020, 07:02

... AccessFTY720 inhibits proliferation and epithelial-mesenchymal transition in cholangiocarcinoma by inactivating STAT3 signaling Zhaoyang Lu1†, Jiabei Wang1†, Tongsen Zheng1†, Yingjian Liang1, Dalong Yin1, ... of Mcl-1, cyclin D1 and Bcl-xL It might also inhibit EMT by up-regulation of E-cadherin and down-regulation of Vimentin and N-cadherin, both in vitro and in vivo Methods Cell lines and reagents ... cyclin-CDK complexes, allowing Rb to become activated and to halt the cell cycle In addition to inducing p16 and p27, FTY720 also downregulated cyclin D1 and cyclin E in CC cells contributing

Ngày tải lên: 30/09/2020, 14:39

... (bone resorption experiexperi-ments) [31] The bones were preincubated for 18 to 24 hours in α-MEM con-taining 0.1% albumin, antibiotics, and 1 μmol/l indomethacin After preincubation, the bones ... Semi-quantitative reverse tran-scription-polymerase chain reaction (RT-PCR) analysis on samples obtained by incubating five bones in control medium and five in medium contain-ing SF (10%) from a patient ... 5h). Effects of neutralising antisera on bone-resorbing activity in SFs The role of different cytokines in SF-induced bone resorption was investigated by incubating mouse bone organ cultures with

Ngày tải lên: 09/08/2014, 10:20

... protein 54 (Tmem54), coiled coil domain containing 28B (Ccdc28b) and testis-specific serine kinase 3 (Tssk3) all to be downregulated Furthermore, we found zink finger and BTB domain containing ... incidence of sialadenitis in congenic mice and healthy controls Congenic regions and incidence of sialadenitis in congenic mice and healthy controls (a) The location of the two nonobese diabetic (NOD) ... Gng10 Guanine nucleotide binding protein (G protein), gamma 10 1.8 16008 Igfbp2 Insulin-like growth factor binding protein 2 1.7 73723 Sh3bgrl3 SH3 domain binding glutamic acid-rich protein-like

Ngày tải lên: 09/08/2014, 10:21

... The clinical S epidermidis strain RP62A was grown in the presence of IQ-143 (concentrations of a quarter of the minimum inhibitory concentration and twice the minimum inhibitory con-centration) ... MsrA (conferring resistance to macrolides, lincosamides, strep-togramins), TetK (conferring resistance to tetracycline), NorA (conferring resistance to fluoroquinolones), VgaAB (conferring resistance ... the combina-tion with metabolic modeling allowed us to fill in miss-ing information on all central metabolic enzymes, including those not affected by significant gene expres-sion changes, and to

Ngày tải lên: 09/08/2014, 22:24

... pro-duction of resveratrol in Asia, particularly in China In Europe, wine is the main source of this substance A variety of stilbenes have been found in wine, including astringin, cis- and piceid and ... damage and the intensity of mycorrhizal colonisation In inoculated F japonica, leaf damage increased piceid content, decreased the intensity of mycorrhizal colonisation and weakened the relation-ship ... only pathogens but also mycorrhizal ones, belong to the stressors capable of induction of such responses in plant cells like chromatin decondensation enabling, besides others, gene expres-sion

Ngày tải lên: 11/08/2014, 11:20

... bacteria by resuspending a single colony in 50 μl of destH20, vortexing, heating at 95°C for 5 min and centrifuging at 12000 × g for 1 min Oneμl of the resulting supernatant containing DNA was used ... reproducing complex field conditions in the laboratory Our understanding on how variations in water tem-perature, oxygen and salinity levels might influence fish welfare and susceptibility for infectious ... difficulties in reproducing complex biotic and abiotic field conditions in the laboratory In this study we looked at the relationship between SAV-infection in salmon and sub-lethal environmental

Ngày tải lên: 12/08/2014, 04:20

... National Institute of Environmental Medicine, Division of Physiology, Karolinska Institutet, Stockholm, Sweden Email: Sofia Jonasson* - sofia.jonasson@medsci.uu.se; Linda Swedin - linda.swedin@ki.se; ... patients no protection against MCh-induced bronchoconstriction [5,6] Most investigations of murine models of airway inflam-mation have focused on bronchial responsiveness and ... major impact on peripheralairway and tissue physiology, protecting against MCh-induced increases in tissue elastance (H) in both animals with acute and chronic inflammation and also in healthy

Ngày tải lên: 12/08/2014, 15:21

... weakness in knee joint osteoarthritis: the effects of prolonged vibration on torque and muscle activation in osteoarthritic and healthy control subjects Health and Rehabilitation Research Institute, ... mechanisms explaining γ-loop dysfunction may be different in different populations Obtaining a better understanding of its underlying causes could have important implications in the rehabilitation of these ... activation of the muscle [19, 46] In support of this conjecture, Konishi and colleagues [32] observed a reduction in maximum quadriceps torque production and EMG amplitude following the injection

Ngày tải lên: 12/08/2014, 18:20

... currently ongoing, is comparing the effects of epinephrine with a combination of a fixed dose of dobutamine in addition to norepinephrine Dopamine alone or versus norepinephrine (Table 1) The infusion ... dobut-amine and norepinephrine Duranteau and coworkers [11] compared the effects of epinephrine, norepinephrine and the combination of norepinephrine and dobutamine in patients with septic shock on ... flow NA Epinephrine, norepinephrine in versus norepinephrine 1 ± 0.6 with epinephrine and random order to achieve MAP versus norepinephrine + norepinephrine + dobutamine, 70–80 mmHg dobutamine 1.1

Ngày tải lên: 12/08/2014, 20:20

... respiration, and organ function in two experimental sepsis models Methods 48 pigs were randomized to continuous endotoxin infusion, fecal peritonitis, and a control group (n = 16 each), and each ... endotoxin high-volume (n = 6, 75%) groups Mortality was higher in high- vs low-volume Figure 1 Continuous and bolus inputs and urine, gastric and ascites outputs for each group Continuous and bolus ... electronic supplement [see Table S1 in Additional Data File 2] Mitochondrial function Sepsis had only limited effects on hepatic mitochondrial respi-ration [see Table S2 in Additional Data File 2 and

Ngày tải lên: 13/08/2014, 20:21

... deter-mined the independent predictors of in-hospital and one-year mortality and assessed the impact of beta-blocker at admission and/or at discharge on outcome. Materials and methods Setting and ... angiotensin-converting enzyme inhibitors; ARB, angiotensin receptor blocker; CI, confidence interval; HR, hazard ratio Trang 6continuation of beta-blocker therapy in patients withADHF was demonstrated ... support CM contributed to conception and design, analysis and interpretation of data, manuscript draft, and critical revision of the manuscript Competing interests Dr Mueller reported receiving research

Ngày tải lên: 13/08/2014, 21:21

... dimensions and weights of front leg muscles, tendons, and bones were determined in 44 gilts that were offspring of three Duroc lines from a five-generation divergent-selection experiment on leg ... age and confined to enclosed pens with concrete floors and flush gutters A 1G% corn-soybean meal grower ration containing appropriate levels of vitamins and minerals was fed to the gilts on a ... heavier in high-line than in low- or control-line pigs In contrast to weight values of the bones, there were several significant line differences in bone lengths The humerus was shorter in high-line

Ngày tải lên: 14/08/2014, 20:20

... contain vast amounts of information including contents,product history, origin and destination points, order information and handlinginstructions RFID can track ‘pedigree’ information where the product ... production and finished goods,important information for shipping and possible recalls RFID-Secondly, for finished goods shipping out the back door, RFID tags on going pallets and containers contain ... reduceoperating costs by reducing labor costs, claims and returns, thereby increasingoperating income And working capital can be reduced by enabling reductions ininventory and lowering inventory

Ngày tải lên: 28/09/2014, 15:37

... propositions including statements and questions and the projection of proposals consisting of offers and commands While direct and indirect speech in traditional grammar emphasize on structure and ... without mentioning three metafuntions: ideational function, interpersonal function and textual function These three metafunctions are interrelated to one another and they are corresponding to clause ... complex, intonation and rhythm and cohesion and discourse These concepts are considered the theoretical foundation for many of his followers to base on in their research and publications Ten years

Ngày tải lên: 02/03/2015, 14:22

... from the China Health and Nutrition Survey (CHNS) We thank the National Institute of Nutrition and Food Safety, China Center for Disease Control and Prevention; the Carolina Population Center, ... are conducted in Section 1.6, and Section 1.7 concludes Trang 151.2 Basic education in rural ChinaIn rural China, basic education is provided almost entirely by local government It normallyconsists ... Liaoning province were not included in our main sample, as we focus oncommunities that were surveyed every wave and the 1997 survey was not conducted in Liaoning.While the middle school attainment

Ngày tải lên: 10/09/2015, 09:09

... cost: Aeration in dual-chamber MFCs brings continuous energy consumption while single-chamber MFCs eliminate this operation with reduced energy consumption This parameter is evaluated in lab-scale ... saturated, and it could also be used in single-chamber MFCs by incorporating membrane-cathode-assembly (MCA) Trang 2410 configuration The main challenge in choosing a membrane is to find one selective ... reducing robustness In addition, the microorganism-based MFCs usually include an aeration system in aqueous cathode compartment, bringing additional operational cost in practical applications

Ngày tải lên: 10/09/2015, 09:29

... 33Interestingly, recent studies have demonstrated up-regulation and increased involvement of COX Va and Vb in a variety of cancers Autocrine gastrins-induced up-regulation in COX Vb resulted in ... biogenesis and activity in response to changing environment or physiological conditions plays a defining role in cellular metabolic adaptation Regulation in COX biogenesis enables the modulation of ... to uncoupling and led to ROS production in CEM cells (Hao, Yu et al 2004) Indeed, we reported increased oxygen consumption in CEM/Neo cells treated in similar fashion However, we reason two possible

Ngày tải lên: 14/09/2015, 08:42

... interaction in the combined transcription translation assay At a TmCSP concentration of 139.5 lm (the TmCSP concentration for 50% inhibition of the protein expression), the protein expression ... RNA chaperones on the protein expression CSPs in different concentrations were used to monitor the concentration dependent effects on gene expression We analyzed the inuence on expression rate ... concentrations in expression as indicated) was incubated with TmCSP (nal concentration was 139.5 lM) for 20 at 25 C and then subjected to CAT expression transcription and or translation by interaction...

Ngày tải lên: 07/03/2014, 21:20

... will continue to rise in the years ahead, due to the high proportion of adults in the region already living with HIV/AIDS, and the continuing difficulties in expanding access to life-prolonging ... food, clothing, shelter, health care and education; Emotional problems, including lack of caring, love, support, space to grieve and containment of emotions; and Social problems, including lack ... manpower, skills and financial constraints, their contributions have been limited and concentrated more in urban and semi-urban areas thus limiting the desired national response to addressing orphan...

Ngày tải lên: 16/03/2014, 10:20