server interaction with files and http

... both files and directories this way 16.3.2.1 Moving files and directories To rename a file and move it to a different directory simultaneously, just replace the last portion of the command with ... command that copies everything in the current directory—both files and directories—into FinishedMovies: cp -R * /FinishedMovies Here's one more example: a command that copies everything (files and ... Mac always keeps them together with their associated files When you're working with files on the command line, however, you have to be more cautious In Leopard (and Tiger too), the most common...

Ngày tải lên: 14/12/2013, 12:15

... Sugihara G (1996) Design and synthesis of amphiphilic alpha-helical model peptides with systematically varied hydrophobic-hydrophilic balance and their interaction with lipid- and bio-membranes Biochemistry ... initial electrostatic interactions, whereas the interaction of PFPs with membranes was most affected by hydrophobic interactions These properties may lead to the selectivity of PCPs and ⁄ or the nonselectivity ... fluorescence intensity achieved by the peptides, and F0 and Ft are the initial fluorescence intensities observed without the peptides and after treatment with Triton X-100, respectively All conditions...

Ngày tải lên: 23/03/2014, 10:21

... fluorescence of compounds and can be used to monitor interactions with DNA and compounds behaviour when encapsulated in liposomes Both compounds and were tested for their interaction with natural salmon ... compounds and are neutral molecules (and electrostatic interaction with the negatively charged DNA molecule is not expected), the high fa values indicate that the main type of interaction with the ... cb/[DNA] and cb and cf the concentrations of bound and free compound, respectively, calculated by Table Maximum absorption (labs) and emission (lem) wavelengths, molar absorption coefficients (ε) and...

Ngày tải lên: 21/06/2014, 03:20

... Microsoft SQL Server version 6.5 and earlier, you must use the OLE DB Provider for SQL Server Furthermore, we specify both the CommandText and the OleDBConnection properties of the OleDBCommand Reference: ... www.testking.com -7- 070 - 310 A myCommand.CommandText = “TestKingCustomer, ALFKI” myCommand.Parameters.Add (“@CustomerID”) B myCommand.CommandText = “TestKingCustomer” myCommand.Parameters.Add (“TestKingCustomer”, ... (“TestKingCustomer”, “ALFKI”) C myCommand.CommandText = “@CustomerID” myCommand.Parameters.Add (“TestKingCustomer”, “ALFKI”) D myCommand.CommandText = “TestKingCustomer” myCommand.Parameters.Add (“@CustomerID”,...

Ngày tải lên: 10/12/2013, 14:16

... cmd.ActiveConnection = cnn cmd.CommandText = strSQL cmd.CommandType = ADODB.CommandTypeEnum.adCmdText ' Execute the command cmd.Execute() ' Reopen the Orders table and redisplay the ShippedDate ... can also use the Insert and Delete statements to perform other bulk operations with the Command object One of the last tasks that is useful to perform using ADO with SQL Server is to create objects ... merely to display the before and after data, as seen in Figure A.6 Figure A.6 Although they're not pretty, you can see the values of the OrderID and DeliveryDate before and after the routine has...

Ngày tải lên: 21/01/2014, 12:20

... of the cofactor interaction domain within ExoS Various single mutant ExoS proteins were tested for their capacity to interact with 14-3-3 and subsequently for their cytotoxicity and ADP-ribosylation ... how the interaction between 14-3-3 and ExoS occurs, our pull-down analysis with GST-ExoS(LDL426– 428AAA) still strongly suggests that ExoS must utilize a strategy for its interaction with 14-3-3 ... anti-ExoS antibodies (D) and (E) Cells were lysed and samples were separated by SDS ⁄ PAGE Western blot analysis was performed on immunoblotted filters with anti-Ras (D) and with anti-Erk (E) antibodies...

Ngày tải lên: 19/02/2014, 08:20

... its complex with the transition state analog S-(2-boronoethyl)-l-cysteine (BEC) His120 and His145, and the corresponding His101 and His126 in arginase I, were described among the ligands for coordination ... undetectable, both before and after the incubation with the manganese ions Fully active His120Asn and His145Asn variants exhibited about 53% and 95% of the corresponding wild-type activity, with the Km value ... interaction of human arginase II with substrate and metal ions was examined by sitedirected mutagenesis and kinetic studies Selection of target residues (Asp149, His120 and His145) was based on the...

Ngày tải lên: 20/02/2014, 02:21

... known modes of a-amylase inhibition A standard colouring scheme is used with enzyme drawn in magenta and inhibitor helix, strand and coil drawn in red, cyan and yellow, respectively The calcium ... activity PPA and HSA 0.19 T aestivum PPA and HSA 0.53 T aestivum HSA and PPA (low) 0.28 WRP25 T aestivum T aestivum PPA and HSA None WRP26 T aestivum None WRP27 T aestivum None 1,2 and BIII S cereale ... 1) and forms a separated folding unit, exclusively made of b sheet Eight of the 10 strands fold into a b sandwich structure with ÔGreek keyÕ topology The conservation of the interface of A and...

Ngày tải lên: 21/02/2014, 03:20

... understanding of the heart as a muscular pump and of the interaction between the heart and the vasculature The concepts of contractility, preload and afterload are paramount to this understanding and ... bundles of myosin strands with each strand having a tail, a hinge and a head region The tail regions bind to each other in the central portion of the filament and the Page of 23 strands are aligned ... understanding and will be the focus and repeating theme throughout the text A sound understanding of cardiac physiology begins with basic understanding of cardiac anatomy and of the physiology of muscular...

Ngày tải lên: 22/02/2014, 09:20

... b-crystallin and d-crystallin behave similarly (show only one band), whereas calmodulin and troponin behave like each other On the other hand, Calnuc and parvalbumin generate both the J-band and the c-band, ... (attenuating both the J-band and the c-band), indicating that the dye binds in the EF-hand motif Binding of Mg2+ caused a decrease only in the c-band, without disturbing the J-band (Fig 7A) Although ... and parvalbumin seem to complex with the dye similarly, and yield the J-band [25] As the dye ⁄ protein ratio is decreased, the J-band is lost in the former two proteins, yielding the b-band and...

Ngày tải lên: 07/03/2014, 00:20

... tRNA and tmRNA alanylation under standard reaction conditions and 50 °C should be close to 0.8 s)1 and 0.03 s)1, respectively This is to be compared with kcat values of 0.93 s)1 [27], 1.1 s)1 and ... mobility shift study of the interaction between EF-Tu*GDPNP and uncharged tmRNA Two equivalent gels were run at identical conditions and stained with Coomassie Blue R250 (A) and with pyronin Y enhanced ... the interaction with similar structural patterns (the alanylated acceptor stem with the specifically recognizable GU base pair, and the T loop) On the other hand, in an established complex with...

Ngày tải lên: 08/03/2014, 08:20

... maps, with the o and coot programs [36,37] The final refinement was performed with refmac from the ccp4 suite [32], with TLS parameterization Both cns and refmac used the same Rfree set (randomly ... dithiothreitol and mm EDTA, and purified further by anion exchange chromatography with a Mono Q column (Amersham Biosciences; 20 mm Tris ⁄ HCl, pH 7.5, containing 10 mm dithiothreitol and mm EDTA, with ... g) and 20 °C RI - RATE complex RI - RNase A complex RI Fig Analysis of the stoichiometry of the RI–SGRSGRSG-RATE complex by native PAGE Lanes and 2: complex of RNase A (100 pmol) with RI (200 and...

Ngày tải lên: 22/03/2014, 16:21

... in the interaction with RNA and DNA in the elongation complex At elevated ionic strength, Rif and r70 suppressed RNAP–aptamer interactions (above) Under low ionic strength conditions, Rif and r70 ... reports on interactions of the core with total cellular DNA and RNA [43,46], tRNA [47,48], ssDNA [43] and also some individual RNA sequences [49] Reported Kd values for some of these interactions ... the aptamers (Fig 5B and data not shown) Specific and nonspecific interactions of aptamers with the RNAP main channel The interaction of the aptamers with RNAP was found to be highly dependent on...

Ngày tải lên: 23/03/2014, 13:20

... them with GST–SAFB1C, GST–SAFB1CDRE, GSTSAFB2C and GST bacterial preparations The beads were washed and the eluted proteins were probed with the anti-GST IgG for SAFB protein detection and the ... These results confirm the direct interaction of SAFBs with the SRPK1 ⁄ 1a proteins and provide a direct link between the affinity of the SAFB–SRPK1 ⁄ 1a interaction and the extent of the inhibition ... substrate with the catalytic region of the kinase, on the one hand, and of the positive charge of the RS domain with the negatively charged ‘docking groove’ of SRPK1, on the other hand The RE-rich...

Ngày tải lên: 30/03/2014, 01:20

... STARD1START is well suited for a ligand with the size and shape of cholesterol [29,30] Critical residues proposed to interact with cholesterol are localized within the fragment containing amino ... yellow, and comprises half of the a3-helix and the strands b3–b7, including their connecting loops The residues Glu107 (E), Arg126 (R) and Leu137 (L) (all marked in magenta) are located within ... region and have been proposed to interact with cholesterol (see Discussion) Otherwise, the model is colored according to the secondary structure, with helices in red, b-strands in green, and loops...

Ngày tải lên: 30/03/2014, 04:20

... isoform and, again, this was stronger that the wild-type protein The dissociation of the G4R and StB-wt was fast and almost complete within min, whereas the dissociation of StB-Y31 was slower and ... (+120 mV) and negative voltage ()120, )100 and )80 mV) The traces are representative of three independent experiments 120 –120 suggests a strong interaction with the membrane in accordance with the ... to another tube and the released calcein measured using a Jasco FP-750 spectrofluorimeter (Jasco Inc., Easton, MD, USA), with excitation and emission at 485 and 520 nm Excitation and emission slits...

Ngày tải lên: 30/03/2014, 04:20

... PIS (lanes 8–12) with increasing amounts of ERRc (0.06 lL in lanes and 7; 0.13 lL in lanes and 8; 0,25 lL in lanes and 9; 0.5 lL in lanes and 10; lL in lanes and 11; lL in lanes and 12) The ERRc–DNA ... tissue culture dishes, washed twice with NaCl/Pi, and harvested in 1.5 mL NaCl/Pi by gently scraping with a rubber policeman Cells were centrifuged with 300 g and the pellet was resuspended in ... stimulating effect is not induced by lipophilic ligand receptor interaction A comparison with the homologous receptors ERRa, ERRb, ERa, and ERb and a data base search in the nonredundant protein...

Ngày tải lên: 31/03/2014, 09:20

... incident laser pulse energies of 2.0 and 2.7 ␮J with 20 nsec pulse width ͑FWHM͒ and a radius of 9.5 ␮m incident on a 0.3 ␮m chromium film A domain with a radius of 12 ␮m and axial depth of 0.9 ␮m is used ... simulations are performed with and without the recoil pressure term in Eq ͑7͒ for both 2.0 and 2.7-␮J laser pulses Due to the time required to perform a simulation with a temperature-dependent ... Nd:YLF laser and exposes the film at the desired time A 35-mm camera captures the illumination of the dye laser and stops the motion of the fluid on film A 600-nm filter with a 40-nm bandwidth is...

Ngày tải lên: 06/05/2014, 08:55

... (lanes and 8), 50 mM (lanes and 9), 100 mM (lanes and 10), 200 mM (lanes and 11), 500 mM (lanes and 12), and 1000 mM (lanes and 13) Lanes 2–7 show the dissociation of the Tat-N-TAR complex, and ... expanding, carry mutations in Tat R57S and G63Q within and close to the basic domain, and yet exhibit increased transcriptional activity [40] On the other hand, the phosphorylation of serines and ... was washed and the HIStagged Tat proteins were eluted with M guanidine-HCl, pH 4.0 The fractions collected were dialysed in 0.1 mM DTT and then analysed by 15% SDS-PAGE and stained with Coomassie...

Ngày tải lên: 18/06/2014, 22:20

... were incubated overnight with rocking at room temperature with clarified antigen preparations and mock-infected controls The blots were then washed with PBS and incubated with the anti-flavivirus ... and infection of monomeric and polymeric peptides containing alpha2beta1 and alphaxbeta2 integrin ligand sequences J Virol 2004, 78:11786-11797 Chu JJ, Ng ML: Interaction of West Nile virus with ... probed with LAMR1 and lamin B1 spe2D gel immunoblots probed with LAMR1 and lamin B1 specific antisera Two dimensional separation of βOGinsoluble NaDOC extracts transferred to nitrocellulose and...

Ngày tải lên: 19/06/2014, 08:20