see criminal personality profiling dna profiling geographic profiling racial profiling

... High-Molecular-Weight DNA from Blood and Forensic Specimens Peter M Schneider 1 Introduction The isolation of genomic deoxyribonucleic acid (DNA) is a crucial step in the process of DNA profiling The ... sufficient amounts of highly purified DNA from biological crime stains as well as from reference blood samples High-mol-wt DNA is usually only required for DNA-profiling protocols based on Southern ... floating DNA pellet from the solution (see Note 4) 4 Rinse the DNA pellet attached to the glass hook twice m 70% ethanol to remove excess salt 5 Dry the pellet briefly in the air and resuspend the DNA...

Ngày tải lên: 11/04/2014, 09:41

... A R C H Open AccessA signal amplification assay for HSV type 1 viral DNA detection using nanoparticles and direct acoustic profiling Yildiz Uluda ğ1 , Richard Hammond2, Matthew A Cooper2,3* Abstract ... nanoparticles under flow, they achieved a detection limit of 10-12M for synthetic DNA sequences Pang et al employed DNA probe-modified 13 nm gold nanoparticles to detect specific sequences from theb-thalassemia ... reduced As can be seen from the examples given in the intro-duction section, the conditions of the hybridisation assay show great variation between applications The length of the target DNA sequence...

Ngày tải lên: 11/08/2014, 00:22

... phylogeographical reconstructions to forensic ana-lyses and species identification [1,2] In plants, many studies have focused on the organelle genomes (i.e., plastid DNA cpDNA and mitochondrial DNA ... to develop new markers for a rapid genomic profiling (by Multiplex PCRs) of cpDNA haplotypes in the Mediterranean olive tree Results: Eight complete cpDNA genomes of Olea were sequenced de novo ... utility of cpDNA variation for forensic analyses is limited to rare haplotypes In contrast, the high cpDNA variation in wild populations demonstrated that our markers may be useful for phylogeographic...

Ngày tải lên: 11/08/2014, 11:20

... Trang 1DNA CHIP PLATFORM: A HIGH-THROUGHPUT GENOTYPING TECHNOLOGY FOR GENETIC DIAGNOSIS AND PHARMACOGENETIC PROFILINGTrang 2THIS THESIS IS DEDICATED TO:Trang ... polymorphisms on the risk of relapse In conclusion, DNA chip platform is a high-throughput and reliable technology forgenetic diagnosis and pharmacogenetic profiling Trang 11LIST OF TABLESPage Table 2.1 ... oligonucleotide spacers 76 Table 3.1 The number of the mutations detected in the 200 reference DNA Table 3.2 Comparison of ssDNA yields using single primer and unbalanced Table 3.3 Respective genotype frequencies...

Ngày tải lên: 12/09/2015, 08:18

... commercially available Qiamp DNA mini Kit (Qiagen) was used to purify the DNA Total DNA was quantified using the ND-1000 spectro-photometer (Nanodrop, Wilmington, DE) Genome-wide DNA methylation profiles ... methylated dsDNA for each sample was amplified using Whole Genome Amplification (WGA, Sigma-Aldrich Co., St Louis, MO, USA) Genomic DNA was used as the control for each sample DNA methyla-tion profiling ... profiles Fourμg of genomic DNA was fragmented by sonication and purified using Mini-Elute columns (Qiagen Co., Hilden Germany), and the amount of double-stranded DNA (dsDNA) was measured using the...

Ngày tải lên: 20/09/2020, 18:46

... the tumour pairs were generally concordant between methylation profiling and aCGH Conclusion: Our results show that DNA methylation profiling as well as aCGH have potential as diagnostic tools ... of the same clonal origin will have closely related DNA methylation patterns and profiles In this study, we set out to compare DNA methylation profiling and aCGH as tools to distinguish between ... CpGenome™ Universal Methylated DNA (Chemicon/Millipore, Billerica, MA) and peripheral blood mononuclear DNA were used as the methylated and unmethylated controls, respectively DNA methy-lation standards...

Ngày tải lên: 28/09/2020, 09:25

... C H A R T I C L E Open AccessRNA-seq profiling of a radiation resistant and radiation sensitive prostate cancer cell line highlights opposing regulation of DNA repair and targets for radiosensitization ... qRT-PCR The DNA repair pathway in radioresistant PC-3 cells was then targeted for radiation sensitization using the PARP inhibitor, niacinimide Results: Opposing regulation of a DNA repair and ... previously been shown to inhibit DNA repair and down-regulate BRCA1 and RAD51 [21] To determine whether radioresistant cells could be rendered sensitive by targeting the DNA repair pathway, the PARP...

Ngày tải lên: 30/09/2020, 14:49

... excerpt from his latest book, published by SAMS—talks about profiling, a concept that I have very rarely seen associated with PHP applications Profiling takes the guesswork out of understanding where ... directory called //sseerrvviiccee This will contain information on all the various services that ddaaeemmoonnttoooollss is running; a program called ssuuppeerrvviissee monitors the //sseerrvviiccee ... ssuuppeerrvviissee to interact with them in an automat-ed fashion Luckily, most applications can be modified so that they can be compatible with ssuuppeerrvviissee, and our ssmmssppaarrsseerr script...

Ngày tải lên: 11/12/2013, 02:15

... by centrifugation cDNA synthesis and gene expression profiling by DNA microarray Alexa-labeled target cDNA was prepared from 20 μg total RNA using a SuperScript Plus Indirect cDNA Sys-tem kit (Invitrogen ... were Table 1 Selection of candidate genes by DNA microarray Total RNA was extracted from each cultured cell line and reverse-transcribed to produce cDNA cDNA samples from cancer cells (labeled with ... Carlsbad, CA, USA) cDNA obtained from cancer cell lines was labeled with Alexa 555, and cDNA obtained from the control cell line was labeled with Alexa 647 The two Alexa-labeled cDNA samples were...

Ngày tải lên: 15/02/2014, 04:20

... therapy, and anti-HER-2 therapy, alone or in combination ER-Gene Expression Profiling Gene expression profiling (see Glossary, Appendix B) is an emerging technology for identifying genes whose ... resulting DNA using PCR The quantification of the DNA produced after each round of amplification is accomplished by the use of fluorescent dyes that intercalate with double-stranded DNA, or by ... intercalate with double-stranded DNA, or by modified DNA oligonucleotide probes (see Glossary, Appendix B) that fluoresce when hybridized with complementary DNA In a PCR template, relative ratios of the...

Ngày tải lên: 06/03/2014, 01:20

... tags) Trang 13of genomic or mitochondrial DNA or unspliced RNA contamination in senger RNA preparations Even using oligo-dT selection and DNAse digestion,DNA and unspliced RNA tends to persist in ... frepresenta-or ing at the cDNA level load-2.1.2 Control for cDNA Loading Relative to Reference Gene With real-time RT-PCR or StaRT-PCR, control for loading is best done atthe cDNA level by amplifying ... Balance cDNA with 6 × 105β-actin CT molecules (the amount of β-actin CT in 1 µL of SMIS) After establishing the amount of cDNA in balance with 6 × 105 copies of β-actin CT, this amount of cDNA is...

Ngày tải lên: 10/04/2014, 22:32

... Journal of Translational Medicine BioMed Central Open Access Research Gene expression profiling for molecular distinction and characterization of laser captured primary lung cancers ... available from: http://www.translational-medicine.com/content/6/1/69 © 2008 Rohrbeck et al; licensee BioMed Central Ltd This is an Open Access article distributed under the terms of the Creative ... candidate genes with diagnostic, prognostic and therapeutic value [6-9] Several gene expression profiling studies in lung cancer have been published, however, it is still difficult to compare...

Ngày tải lên: 18/06/2014, 15:20

... was completed at 42°C for 1 h Full-length dsDNA was synthesized incubating the pro-duced cDNA with 2 U of RNase-H (Promega) and 3 μl of Advantage cDNA Polymerase Mix (Clontech), in Advan-tage ... Double-stranded cDNA was prepared from 3 μg of total RNA (T-RNA) in 9 μl DEPC -treated H2O using the Super script II Kit (Invitrogen) with a T7-(dT15) oligonucleotide primer cDNA synthesis was ... Polymerase Mix (Clontech), in Advan-tage PCR buffer (Clontech), in presence of 10 mM dNTP and DNase-free water dsDNA was extracted with phenol-chloroform-isoamyl, precipitated with ethanol in the presence...

Ngày tải lên: 18/06/2014, 15:20

... digestion and final RNA purification was carried over as follows Sixty ul DNase master mix (containing 6 ul 10× DNase buffer, 4 ul DNase, 50 ul nuclease free water) was added to the center of the filter ... informa-tion that is complementary to that derived from mRNAtranscriptional profiling Thus, comprehensive micro-RNA expression profiling can help to unravel these mas-ter regulators of gene expression, ... functions(see discussion-invasion and metastasis) Interestingly the highest expression of miR-10b wasobserved in nodular melanoma (AM8), invasive melano-mas (AM6, AM9) and desmoplastic melanoma (AM7)(see...

Ngày tải lên: 18/06/2014, 16:20

... specificity of DNA-based hybridization [19,20] In these methods, all transcripts, including globin, are amplified to produce complementary cDNA It is believed the high specificity of DNA-DNA interactions ... profiling from whole blood Many of these used similar methodologies for objectively measuring expression profiling performance [14,22] Others have noted the benefits of usincDNA targets for profiling, ... amelio-rated through the highly-specific properties of DNA: DNA binding Most microarray platforms typically utilize a fixed probe length of DNA, whether spotted in place or synthesizedin situ This...

Ngày tải lên: 18/06/2014, 16:20

... responsible for tumour-like growth of rheumatoid synovium [24] In this study a cDNA array membrane containing 588 cDNA fragments of known cancer-related genes was used to compare the gene expression ... distinct cells that are associated with disease characteristics and subtypes, Review Transcription profiling of rheumatic diseases Lisa GM van Baarsen1, Carina L Bos1, Tineke CTM van der Pouw Kraan2and ... to gain insight into the molecular complexity of cells and tissues has arisen with the advent of DNA microarray technology, which facilitates open-ended survey to identify comprehensively the fraction...

Ngày tải lên: 09/08/2014, 01:22

... enhanced with fluorescently labeled anti-streptavidin antibodies DNA chips were scanned to obtain quantitative gene expression levels DNA chip hybridization, Fluidics Station operations, scanning, ... and imported into DNA-Chip Analyzer version 1.3 [45] Data were normalized, and expression values, based on the perfect match/mis-match (PM/MM) model, were calculated for each DNA chip All chips ... expression values for each DNA chip were combined into a single file (three chips × four experimental conditions × three to five replicates), and imported back to DNA-Chip Analyzer; the resulting...

Ngày tải lên: 09/08/2014, 06:22

... was used to validate microarray data Before cDNA synthesis, RNA sam-ples were DNase treated to remove genomic DNA contamina-tion by using Ambion's DNA-free Kit (Ambion Inc., Austin, TX, USA), ... biomarkers for RA More-over, gene expression profiling in animal model holds great promise for our understanding of human pathogenesis For example, profiling gene expression in a rat model of ... biomarkers by transcript profiling of peripheral circulating lymphocytes As an initial proof-of-concept, we demonstrated the feasibility of such technology by successful profiling PBMCs in a rat...

Ngày tải lên: 09/08/2014, 07:20

... described [21], and the resulting, labelled cDNAs were imme-diately used for hybridisation Transcriptome analysis and qRT-PCR Our array covering 12,000 cDNA probes for 10,000 non-redundant genes ... arrays were made from a single batch of cDNA probes Every RNA sample was hybridised at least twice on separate arrays Whenever necessary, the sequence of cDNA probes was con-trolled with an ABI3100 ... significantly differ between the paired subsets 1 and 2 Gene profiling in pre-treatment PBMCs correlates with treatment responsiveness Gene profiling in PBMCs was studied in the two training sub-sets...

Ngày tải lên: 09/08/2014, 08:22

... 1Open AccessVol 8 No 4 Research article A model of anti-angiogenesis: differential transcriptosome profiling of microvascular endothelial cells from diffuse systemic sclerosis patients Betti Giusti1, ... article is online at: http://arthritis-research.com/content/8/4/R115 © 2006 Giusti et al.; licensee BioMed Central Ltd This is an open access article distributed under the terms of the Creative ... factor activation [4] and release of ECM-trapped regulatory molecules [5,6] While gene-expression profiling using microarray technologies is available for skin biopsies [7] and cultured fibroblasts...

Ngày tải lên: 09/08/2014, 08:22