screening in high density formats

... High-density Stocking in a Recirculating Aquaculture System on Gill BJ Smith, S.A Smith, and CJ Pfeiffer Aquatic Medicine Laboratory Virginia-Maryland Regional College of Veterinary Medicine ... 15 control fish were maintained in their original system, in which the stocking density remained low From the experimental group, 15 fish were arbitrarily selected to investigate the reversibility ... The remaining fish were divided into two groups and placed in recirculating systems where they were reared for eight months One group (control; n =21) was maintained at low population density

Ngày tải lên: 27/09/2019, 10:20

... of cotton using the RAD-Seq method and the map linkage 3984 markers with a total distance of 3499.69 cM In this study, a recombinant inbred line (RIL) popula-tion, containing 196 individuals ... information of sequencing data in each line in the whole RIL population a Distribution of the number of markers in each line of the whole RIL population b Distribution of the average sequencing ... results of this research would provide useful information for the next-step work including fine mapping, gene functional analysis, pyramiding breeding of functional genes as well as marker-assisted

Ngày tải lên: 22/05/2020, 04:04

... morphology, number of infloresinflores-cences per shoot, flowering date [26], timing and duration of flowering and of veraison, veraison-ripening interval [14,44], architecture of the inflorescence [45], ... setting, and environmental factors (e.g rainfall, hail storms), the number of individuals bearing fruits var-ied from year to year Vines were planted in 2008, without replicate, in the vineyard ... beginning of the vegetative period (late spring) The samples were immediately stored in liquid nitrogen and transferred to a freezer maintained at −80°C Sam-ples, weighing 0.5 g were ground in

Ngày tải lên: 26/05/2020, 23:45

... widely in China while the female parent ‘Miaoqianzhima’ is a landrace accession originating from Anhui province in China The two varieties are distinct in many morpho-logical traits, including plant ... markers combined with 1,195 previously reported EST-SSR or genomic-SSR and 79 InDel markers [31], were used to construct a high-density genetic map of sesame using a recombinant inbred line (RIL) ... so far most high-density genetic map of 14 LGs in combination with SSR and InDel markers Using this RIL population and genetic map, several grain yield-related QTLs had been detected in more than

Ngày tải lên: 27/05/2020, 00:10

... the main enriched GO terms were cellular process, protein metabolic process, protein modification process, macromolecule modification, nucleoside bind-ing, lipid bindbind-ing, ATP bindbind-ing, ... by linkage mapping in three RIL populations The lines linking loci denote epistatic associations between QTLs Blue lines denote links between two QTLs on different chromosomes, while red lines ... mapping population c Chr indicates chromosome Major QTLs are shown in bold d Interval indicates the confidence interval between two markers e Position indicates the physical position of the interval

Ngày tải lên: 28/06/2021, 20:42

... tradeoff space between independent variables First, increasing thrust can be done by increasing power, without introducing a penalty on delta-V Likewise, increasing propellant density has a direct ... species (assuming single ionization) can be written in terms of accelerating voltage as in Equation 1.2.7 This can be further described in terms of a specific propellant species, shown in Equation ... is inextricably intertwined with propellant density and so these are collectively determined by propellant choice The tradeoff space between molar mass and accelerating voltage is then determined

Ngày tải lên: 24/10/2022, 01:51

... risk, such as earlier initiation of screening, shorter screening intervals, or the addition of screening modalities such as, breast ultrasound or magnetic resonance imaging (MRI) other than mammography ... findings as mass number, Mass form (round, oval, polygonal, linear, branching or speculated), Mass margin (well defined or indistinct), enhancing pattern (homogenous, septated, inhomogeneous or ring ... classified according to their clinical pre-sentations into two groups: Group 1: Cases with a past history of breast carcinoma Group 2: High risk patients coming for regular annual screening A thorough

Ngày tải lên: 02/11/2022, 09:25

... genotyped using the Illumina Bovine SNP50 BeadChip (Illumina Inc., San Diego, CA) In total, 557 bulls in the EuroGenomics project (Lund et al., 2011) were re-genotyped using the Illumina Bo-vineHD ... (MGS) in training data set; Groupsire: bulls had sire but no MGS in training data set; Group mgs : bulls had MGS but no sire in training data set; Group non : bulls had neither sire nor MGS in ... maternal grandsire (MGS) in the train-ing data set (Group smgs); (2) bulls that had sire but no MGS in training data set (Group sire); (3) bulls that had MGS but no sire in training data set (Group

Ngày tải lên: 02/11/2022, 14:34

... 5q15-35.3 and 5q14.3 in high-grade colorectal adenomas LOH was not detected in either low-grade or high-grade col-orectal adenomas In intramucosal adenocarcinomas, regions of gain defined in more than ... molecular alterations in high-grade adenoma are similar to those in intramucosal adenocarcinoma at the level of chromosomal instability [16, 17] In addition, this finding is highly useful to predict ... tumour In fact, distinguishing adenomatous lesions (in particular, high-grade adenoma) from intracarcinomatous lesions on the basis of surface macroscopic findings is very difficult We will examine

Ngày tải lên: 04/12/2022, 15:34

... caudal peduncle; 8, Origin of caudal fin membrane on ventral midline; 9, Posterior insertion of anal fin; 10, Anterior insertion of anal fin; 11, Insertion point of pelvic spine into the pelvic girdle; ... between pond and marine nine-spined sticklebacks–includ-ing the two populations used in this study–has suggested this divergence has a genetic basis31 Among other things, marine nine-spined sticklebacks ... participate in several pathways, but none of them is involved in the Eda-pathway (cytokine-cytokine receptor interaction pathway) controlling for lateral plate number variation in the three-spined

Ngày tải lên: 04/12/2022, 16:06

... sequencing (RAD-seq), which can provide abundant InDel and SNP markers to construct high-density genetic map [39–41] In the present study, a high-resolution genetic map was constructed in a recombinant ... cloned in wheat For example, the appli-cation of semi-dwarfing genes Rht-B1b and Rht-D1b not only effectively improve the lodging resistance but also improve the harvest index, resulting in increasing ... [11, protein kinase glycogen synthase kinase3 and indepen-dently control semispherical grain trait [13] A jasmonic acid synthetic gene keto-acyl thiolase 2B was cloned in a TGW mutant, showing significant

Ngày tải lên: 30/01/2023, 20:47

... two being smaller when tree size increases real volume It follows from the on where 0.2618is versus Wood In a infradensity direct stem volume index to dry mass conversion, the wood infradensity ... Within the interest of land set aside programmes in industrialized countries, a joint European research program was initiated as a collaborative study between the Universities of Antwerpen, Edinburgh ... differs olating Volume index versus as soon as dry mass The estimation of stem dry mass by means of volume index data integrates incertainties due to both real volume estimation and In other respects,...

Ngày tải lên: 08/08/2014, 18:21

... intersect with similarly high- ranking ext AUC values in the other groups, with more than 75% of outlier peaks in JPT and CHB intersecting with high- ranking ext AUC values in the other East Asian ... possessing both high ranking extAUC values and high average Fst/θ between populations (abbreviated as a high/ high peak); and peaks with high- ranking ext AUC values but low average Fst/θ (a high/ low ... extrema To delineate the extremes of local variation in ext AUC values, we divided each population’s values by determining local peaks and valleys in the data To determine inflection points in the values,...

Ngày tải lên: 09/08/2014, 22:23

... proteins is the need to achieve high cell density while maintaining high culture viability in order to obtain high recombinant glycoprotein yield and quality In most of these proceses, batch ... ‘knocking’ bioreactor principles into my brain and his ‘weird’ insights, Yih Yean for his endless help and fish-tales, Vesna for keeping things in perspective, Niki for her friendship and inputs ... containing two to four cysteine-rich pseudo-repeats, a single transmembrane region and a conserved intracellular protein-protein interaction domains called death domain (Curtin and Cotter, 2003) Upon...

Ngày tải lên: 15/09/2015, 17:10

... improvement in culture cell density for CHO GT cell lines in FBC translated into improvement in recombinant protein yield Compared to the 20 mg/L of recombinant human IFN-g yield in CHO parental ... was inserted with a pair of oligonucleotide insert containing unique Requiem sequences shown by underlined nucleotides 352 Cell Line CHO IFN-g is a suspension Chinese Hamster Ovary cell line ... Relative increase or decrease in expression in various cell lines compared to the parental CHO IFN-g cell line as determined by quantitative real-time PCR using primers specific for the genes examined...

Ngày tải lên: 15/09/2015, 17:10

... signal-regulating kinase Activating transcription factor Baculoviral IAP repeat-containing protein 1/neuronal apoptosis inhibitory protein Baculoviral IAP repeat-containing protein 4/X-linked inhibitor ... signaling genes in cell culture In this article, we examined the apoptosis signaling pathways induced during BC and FBC of a Chinese Hamster ovary (CHO) cell line producing recombinant human interferon ... repeat-containing protein 4/SURVIVIN Bruton agammaglobulinemia tyrosine kinase Caspase and FADD-like apoptosis regulator, Cflar Caspase Gadd153 Direct IAP binding protein with low PI mRNA Death inducing...

Ngày tải lên: 15/09/2015, 17:10

... glutamine setpoint fed-batch instead (Fig 1A) This pointed to increased glutamine consumption during implementation of additional glucose control Indeed, the specific glutamine consumption in the ... glutamine and glucose concentrations compared to batch so that feeding could be initiated earlier at f15 –18 h after seeding Once feeding had been initiated, glutamine concentrations can be maintained ... yet high enough to prevent severe nutrient limitation In addition, we found that maintaining a fixed glucoseto-glutamine ratio allowed for an indirect method of limiting glucose uptake Since...

Ngày tải lên: 15/09/2015, 17:10

... today not contain the minimum distance constraint anymore, as this results in a lower code rate, which is costly when considering the high data rates in high- density magnetic recording Code rates ... counterparts This thesis focuses mainly on nonlinearities found in longitudinal recording A dominant and well known nonlinearity found in magnetic recording is termed nonlinear transition shift (NLTS) ... PRECOMPENSATION IN HIGH- DENSITY MAGNETIC RECORDING LIM YU CHIN, FABIAN (B Eng (Hons.), NUS) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF ENGINEERING DEPARTMENT OF ELECTRICAL ENGINEERING NATIONAL...

Ngày tải lên: 27/11/2015, 12:23

... quarter in the middle, still has the highest density) New Towns in the south-west of Hanoi (including Linh Dam) have been rapidly developed in many types and forms, contributing very much in the ... expansion underlying urbanization in Hanoi in general and Hanoi New Towns in particular Getting along with the rapid expansion, infill growth has occurred with high density in the south-west ... population density, road and housing densities Urbanization results in the expansion of administrative boundaries, stretching in various directions and leading to changes in land use, mainly at...

Ngày tải lên: 29/08/2013, 08:15

... bovine serum (FBS, hyClone, Utah, USA) containing 100 UÆmL)1 penicillin/streptomycin and mM L-glutamine at 37 °C under 5% CO2 The JAr and Jeg3 cell lines (ATCC) were maintained in Dulbecco’s minimum ... Results Binding of HDL to choriocarcinoma cells Specific binding of HDL3 to all three choriocarcinoma cell lines at °C was saturable at the highest lipoprotein concentrations (100 lg proteinÆmL)1) ... scanning microscopy Immunofluorescence was performed on choriocarcinoma cells (24 h in DMEM containing 10% FBS, 1% glutamine and 1% penicillin/streptomycin) and CHO cells (HAM’s F12 medium containing...

Ngày tải lên: 20/02/2014, 23:20