scientific workflow optimization in e science

... temperature was measured by reading every 0.2 °C for sec from 95 °C to 60 °C Table 2-8 Primer sequences for PCR of cell cycle regulating genes and reference genes Annealing Gene bank Gene Sequence ... humidified atmosphere containing 5% CO2 Cells were passaged every week and seeded at a density of × 103 cells/cm2 in 80 cm2 cell culture flasks For cell freezing, × 106 cells in freezing medium were ... fluorescent dye that binds the DNA is added to a suspension of permeabilized single cells or cell nuclei The stained material is then measured in the flow cytometer and the emitted fluorescence...

Ngày tải lên: 19/11/2015, 15:51

... The findings are presented in Table While only 20% of the 60 minutes irradiated larvae survived for days, the control experiment showed that 100% of the larvae were alive during the same period ... rate of the larvae By this time the larvae has developed to the infective third stage larvae Larval development test The test was carried out in a 90 mm diameter petri dish 100 µl of the egg ... an increasing UV irradiation exposure is in line with the findings of Samuelson et al., (1984) The decrease hatchability in eggs expose to high level of (60 minutes) of radiation might have been...

Ngày tải lên: 07/08/2014, 17:22

... work, interpreted the data and helped drafting the manuscript All authors read and approved the final manuscript Competing interests The authors declare that they have no competing interests Received: ... Icycler (Bio-Rad, Hercules, CA) at a melting temperature of 95°C for 15 sec and an annealing temperature for for 40 cycles The primer sequences and annealing temperatures for the six genes studied ... cytometry (FCM, BD Biosciences, USA) was Page of performed with PI and fluorescein isothiocyanate (FITC)-labeled Annexin V (Joincare Biosciences, Zhuhai, China) [10] After treatment, the remaining...

Ngày tải lên: 09/08/2014, 09:20

... between regions were created using numerical weight coefficients The degree of association between the two matrices indicates the degree of fit of the model (Fortin and Gurevitch 1993, Legendre ... coefficients were also computed using the real number of degrees of freedom, according to the level of autocorrelation present (Garrett and Toulany 1981) The difference between the levels of mean ... Synchrony levels obtained after correcting the number of degrees of freedom were similar to the levels obtained after first-differencing Synchrony in Pre-Fishery Abundance The level of synchrony in PFA...

Ngày tải lên: 13/11/2014, 09:14

... once, immediately after the stress exposure Mitogen responsiveness The mice anesthetized with halothane were sacrificed and thereafter the thymuses were removed in a sterile manner The thymocyte ... after the restrained stress exposure The thymuses and spleens were removed and placed in disposable Petri dishes containing sterile, ice-cold PBS The suspended cells were released from the lymphatic ... thymocyte levels and CD4+, CD8+ and CD19+ splenocyte levels were determined, each time scoring 300 cells Production of interleukin-1 (IL-1) Mice were anaesthetized with halothane Peritoneal exudate...

Ngày tải lên: 07/08/2014, 18:21

... the textbook Basing on those findings, some recommendations for implementing pre-listening activities were provided They were improving the prelistening activities in the material and using pre-listening ... the survey questionnaire Besides, the researcher conducted class observations in three classes to support the findings The results showed the current situation of teaching and learning prelistening ... prelistening stage at Nguyen Gia Thieu high school, the students’ preferences for pre-listening activities and the teachers’ and the students’ comments on the pre-listening activities available in the...

Ngày tải lên: 19/03/2015, 10:37

... more difficult 91% of the students agree that pre-listening activities make the lesson more interesting and they themselves feel much more interested in the lesson The second ranked purpose is ... Research question To implement pre-listening activities, teachers use many different techniques Yet, some are more frequently used than the others Here are the most frequently used techniques ... part in the lesson and listen effectively through interesting and suitable pre-listening activities In order to achieve these objectives, four research questions are raised: What are the teachers...

Ngày tải lên: 15/07/2015, 13:01

... ones such as neurodegenerative diseases As axons are usually severed or degenerated in these instances, recovery of function is therefore greatly dependent on the ability of the axons to regenerate ... day E. coli Escherichia coli EAE experimental autoimmune encephalomyelitis EDTA ethylenediaminetetraacetic acid EGFR epidermal growth factor receptor EOR ER overload response ER endoplasmic reticulum ... be expressed in the cell bodies and processes of oligodendrocytes, including the outer myelin sheath surrounding myelinated axons, and at the inner adaxonal sheath where myelin and axon came into...

Ngày tải lên: 11/09/2015, 10:06

... and proteins resulting from selected up-regulated genes were further investigated by Western blotting Intracellular generation of ROS was detected by 2’, 7’-dichlorofluorescein diacetate (DCFDA), ... tissue tolerance once defined only for radiotherapy alone should be redefined in chemo-radiotherapy Based on a ROSrelated apoptotic model, there is the possibility of preventive strategies targeted ... showing a computer generated isodose plan 64 Box-plots to compare pre-treatment median sensori-neural hearing thresholds in the lower speech frequencies with the corresponding values at different...

Ngày tải lên: 14/09/2015, 10:43

... nerve toward the newly generated hair cells, suggesting that the new cells attracted the neurons Therefore, the nonsensory 43 cells in the mature cochlea retain the competence to generate new ... often not well kept or easily interpreted Not only was hearing not often considered important in the face of life-threatening diseases, hearing losses were frequently not recorded in those who ... when fetal mouse neural stem cells were transplanted into the mouse inner ear after drug-induced injury, some of the grafted cells differentiated into glial or neural cells whereas other cells...

Ngày tải lên: 14/09/2015, 10:44

... electrons We perform the integral with respect to t2 ; Next, we perform the integral with respect to t of Eq (6) The expression for the unbalanced electron distribution function can be written ... NGUYEN QUANG BAU are the intensity and frequency of a weak EMW; ϕ1 is the phase difference between two electromagnetic waves, Jk (x) is the Bessel function II.2 Calculations of the absorption coefficient ... motion of electrons is confined along the z direction in a DSL, we only consider the in - plane (x,y) current density vector of electrons j⊥ (t) The AC of a weak EMW by confined electrons in the DSL...

Ngày tải lên: 30/10/2015, 20:52

... selenite Error bars represent the standard error based on three independent experiments The Wilcoxon matched-pair test was used to compare effects between control ⁄ treatment experimental set-ups: ... No time-dependent effects were observed, as the activity of both enzymes decreased in parallel with the duration of incubation for both treated and non-treated cells, reflecting varying expression ... important redox enzymes in the endogenous defence against oxidative stress As ROS are continuously formed in all aerobic organisms during metabolism, there is a need for effective defence systems that...

Ngày tải lên: 30/03/2014, 02:20

... barrier of clam development However, the increasing of the biomass gained as well as final production indicated the benefit can be obtained if the appropriate stocking biomass was determined The economic ... in the experiment site were daily monitored at designated points within the experimental area Growth of clam, expressed in mean of shell length (cm) and mean of live weight (g), was determined ... reduction trend when increasing the stocking biomass There was no significant difference between T1 and T4 was detected This is due the fact that the increase in biomass negatively affected the growth...

Ngày tải lên: 21/06/2014, 05:20

... The experiment site was situated in the intertidal areas near the estuary where the clams have been already cultivated for recent years The environment factors such as DO, water temperature, ... the bottom If the clam biomass levels increase beyond a certain level, the natural feed might not be enough for growing Moreover, in the same size treatments, increasing biomass leads to increasing ... salinity (Refractometer) of water in the experiment site were daily monitored at designated points within the experimental area Growth of clam, expressed in mean of shell length (cm) and mean...

Ngày tải lên: 22/06/2014, 12:20

... abnormalities on the foreleg and /or hindleg were counted as one Abnormalities of the extremities were more frequent than cleft palate after irradiation These results are in agreement with earlier studies ... observed for any externally detectable developmental anomalies Fetuses were weighed individually and the mean fetal weight of the individual group litter was calculated Fetuses weighing less ... were checked for external malformations under dissection microscope Fetuses were fixed in Bouin's solution, then stored in 70% ethanol The presence of visceral malformations was determined using...

Ngày tải lên: 07/08/2014, 14:23

... Kriegel et al [12] Small head size has been reported to be a prominent effect in the Japanese children exposed during the 4-17 weeks of gestation [15] Significant decrease in head size was a feature ... of the extremities in live fetuses rose (Table 2) Other anomalities were observed in any of the exposed groups Table Observation on the mouse fetuses 18 days after exposure to different doses of ... the snout to the base of the skull was recorded as head length The distance between the two ears was recorded as head width Measurements were made with a vernier callipers All fetuses were checked...

Ngày tải lên: 07/08/2014, 15:20

... not detected in these experiments Fucoidan treatment increases the expression of surface markers in BMCs BMCs are one of the main immune cell reservoirs in hosts To determine the recovery effects ... then irradiated once (1 Gy) After 24 h, cells were harvested and used for the cell death measurements Annexin V-FITC/PI staining and rhodamine 123 staining were used to confirm the protective effects ... fucoidan-treated BMCs produce high levels of cytokines related to immune responses IL-12 and TNF-alpha were selected for this study as they are representative cytokines which are involved in cell-mediated...

Ngày tải lên: 07/08/2014, 23:22