role of ca 2 in cell regulation
Global targetome analysis reveals critical role of miR-29a in pancreatic stellate cell mediated regulation of PDAC tumor microenvironment
... laminins, including laminin1 (LAMC1), a major ECM component [26] Further, ECM in interaction with cellular integrins forms a scaffold, and plays essential role in cell proliferation, migration/invasion ... of dysregulated miR-NAs in stromal cells with progression of different types of cancer, including pancreatic cancer, indicating the poten-tial of miRNAs in developing targeted therapies [15–20] ... PDAC cell lines [21,39] In addition, Table 3 Pathways enriched for downregulated genes in miR-29a overexpressing hPSCs Cell cycle GINS1; PLK4; TOP2A; GINS2; BLM; CDCA5; PLK1; HJURP; CASC5; ESCO2;
Ngày tải lên: 06/08/2020, 05:36
Báo cáo y học: "The role of codon selection in regulation of translation efficiency deduced from synthetic libraries" potx
... end of nonstop mRNA generated in response to ribosome stalling Rna 2006, 12:248-255. 20 Hengen P: Purification of His-Tag fusion proteins from Escherichia coli Trends Biochem Sci 1995, 20:285-286. ... 20:285-286. 21 Ingolia NT, Ghaemmaghami S, Newman JR, Weissman JS: Genome-wide analysis in vivo of translation with nucleotide resolution using ribosome profiling Science 2009, 324:218-223. 22 Brandt ... Mathematical General 2004, 37:2105. 13 Kudla G, Murray AW, Tollervey D, Plotkin JB: Coding-sequence determinants of gene expression in Escherichia coli Science 2009, 324:255-258. 14 Welch M, Govindarajan
Ngày tải lên: 09/08/2014, 22:23
Báo cáo y học: "The role of Qa-2, the functional homolog of HLA-G, in a Behcet''''s disease-like mouse model induced by the herpes virus simplex" pptx
... the in vivo injection of Qa-2 siRNA reduced the Qa-2 mRNA and protein levels in PBMC of BD mice and deteriorated BD symptoms Taken together, these findings indicate that down-regulation of Qa-2 ... domain was injected into BD mice Five to six individual BD mice in each group were intravenously injected once Figure 2 Qa-2 siRNA reduced Qa-2 mRNA and the frequency of Qa-2 positive cells in ... while it was 94.23 ± 3.86% of glucose injected control mice in lymphocytes (p = 0.029) In gran-ulocytes, the frequency of Qa-2 positive cells was 42.18 ± 28.40% in Qa-2 siRNA injected mice, while
Ngày tải lên: 11/08/2014, 03:20
Báo cáo y học: " Role of Tax protein in human T-cell leukemia virus type-I leukemogenicity" pps
... proteins; calerticulin and calnexin Calerticu-lin is a calcium-binding protein that participates in cal-cium signaling and linked to activation of thetranscription factor nuclear factor of activated ... domain through its kinase-inducible domain (KID) [62] and the p300/CBP-associated factor (P/CAF), which binds through it carboxy terminus to a distinct site located around amino acid 318 to 320 of ... hundreds of Tax modulated cellular genes [82,134] Some of them are directly involved in activation of T-cells proliferation, such as interleukin 2 (IL-2) [135] and the α subunit of its receptor (IL-2Rα)
Ngày tải lên: 13/08/2014, 13:20
Báo cáo y học: "The global role of ppGpp synthesis in morphological differentiation and antibiotic production in Streptomyces coelicolor A3(2)" pdf
... M600 12 – 120 h 12 – 120 h chp genes M570 M600 12 – 120 h 12 – 120 h whiE cluster M570 M600 12 – 120 h 12 – 120 h whiI M570 M600 12 – 120 h 12 – 120 h bldD M570 M600 12 – 120 h 12 – 120 ... 12 – 120 h 12 – 120 h bldM M570 M600 12 – 120 h 12 – 120 h act cluster M570 M600 12 – 120 h 12 – 120 h red cluster M570 M600 12 – 120 h 12 – 120 h SCO4118 M570 M600 12 – 120 h 12 – 120 ... NI60 VEC 2) 0 VEC 2) I60 VEC 2) NI60 1 2 3 2 3 1 2 3 1 2 3 2 3 1 2 3 1 2 3 2 3 1 2 3 2 3 1 2 3 1 2 3 2 3 R1 R2 R3 R1 R2 M653 M667 (a) (b) Trang 10ated with the observed over-expression of the ECF
Ngày tải lên: 14/08/2014, 08:20
THE REGULATORY ROLE OF MATRIX METALLOPROTEINASES IN T CELL ACTIVATION
... abrogated in MMP9 deficient T cells in the presence of calcium-containing media 82 Figure 25 Ionomycin-induced calcium flux is down-regulated in SB3CT treated CD4+ T cells in calcium containing media ... receptor-bound protein 2 Trang 23xxiii IL-21 Interleukin-21 IL-22 Interleukin-22 IL-23 Interleukin-23 IFN-γ Interferon gamma ITK IL-2 inducible T cell kinase IP3 Inositol 1, 4, 5 triphosphate IPF Idiopathic ... CD8+ T cells in calcium-containing media 75 Figure 21 RAS signaling in T cells 77 Figure 22 Total and phosphorylated MEK1/2 protein levels are maintained in SB3CT or vehicle treated CD8+ T cells
Ngày tải lên: 24/08/2014, 11:37
the role of fgfr3 mutation in tumour initiation, progression and invasion of urothelial cell carcinoma in mice
... Bladder 20 1.1.1 The Urothelium 22 1.1.2 Urothelial lineage and stem cells 24 1.2 Bladder cancer 26 1.2.1 Epidemiology 26 1.2.2 Causes 26 1.2.3 Types of bladder cancer 27 1.2.4 Symptoms 27 ... 1.2.5 Diagnosis 28 1.2.6 Treatment 28 1.2.7 Prognosis 29 1.2.8 Pathology of urothelial cell carcinoma 29 1.2.9 Genetics behind bladder cancer 34 1.2.10 Model of two independent pathways of ... assay 215 6.8.2 Development of an ex vivo assay to test the effects of therapeutic drugs 217 6.9 Discussion 229 6.9.1 Recombination 229 6.9.2 In vivo imaging 231 6.9.3 AdenoCre 232 6.9.4 In
Ngày tải lên: 22/12/2014, 20:56
Role of phospholipase a2 in orofacial pain and synaptic transmission 2
... zero Ca2+ conditions, or treatment of cells with lanthanum chloride and recording in external solution containing Ca2+, resulted in attenuation of sPLA2-III induced exocytosis.sPLA2-III also induced ... thus indicating its role in pain transmission (Titsworth et al 2007) After the N- and C-terminal of sPLA2-III are proteolytically cleaved, it allows for the production of sPLA2 only domain in almost ... secretion of the sPLA2 domain of the sPLA2-III into the extracellular space (Murakami et al 2005) The presence of either C or N or both terminals of the sPLA2-III results in the cytoplasmic localization
Ngày tải lên: 10/09/2015, 08:34
The role of ORF3 protein in the molecular pathogenesis of porcine circovirus 2 infection
... protein prevents p53 from binding pPirh2 in vitro and in vivo…………………………………………………… 3.3.2 ORF3 protein alters the subcellular localization of pPirh2…. 71 76 3.3.3 Mapping of the minimal domain of ... growth kinetics of PCV2 108 Figure 26 Role of ORF3 in the release of PCV2 from infected cells……… …111 Figure 27 Role of ORF3 induced apoptosis on the In Vivo spread of ... expression of ORF3 are infectious and replicate in cells in vitro, but not cause apoptosis of the infected cells. The ORF3 of PCV2 has been shown to be involved in the pathogenesis of the virus in mice
Ngày tải lên: 10/09/2015, 08:38
The role of b cells in the pathogenesis of atherosclerosis 2
... plasma cells in the bone marrow of apoE-/- mice Because we maintained the mice on BrdU in drinking water for one month, the result is also indicative that these plasma cells accumulated in the ... migration into the bone marrow Indeed, we observed a statistical significant increased population of IgM+CD138+EdU+B220- in terms of relative percentage and number in the bone marrow of apoE-/-, ... (Figure 22B, 22C & 22D) Thus, our data suggests that mRNA expression of ebi2 may not account for the robust extrafollicular responses in the spleen of apoE-/- mice Furthermore, 109 the increased
Ngày tải lên: 10/09/2015, 09:24
The role of interferon gamma in regulating antigen specific CD8 t cell responses in a mouse model of influenza
... 40 2.1.1 2.1.2 2.1.3 2.1.4 2.1.5 2.1.6 2.1.7 2.1.8 2.1.9 2.1.10 Complete DMEM for cell culture 43 2.1.11 2.2 Mice 43 2.2.1 2.3 Influenza virus 44 2.3.1 2.3.2 2.3.3 2.3.4 Trang 82.4 Cell ... assays 63 2.8.1 51Cr release assay 63 2.8.2 2.9 Reverse Transcription 65 2.9.1 2.9.2 2.9.3 2.9.4 2.10 Lung Histology 67 Trang 92.10.2 2.10.3 2.10.4 2.10.5 2.10.6 2.11 Statistical Analyses ... 49 2.4.1 + T cells from spleens and lymph nodes of nạve mice 49 2.4.2 + T cells and adoptive transfer 50 2.4.3 infected mice 51 2.4.4 2.4.5 2.5 Flow cytometry and cell sorting 54 2.5.1 2.5.2
Ngày tải lên: 10/09/2015, 09:25
Elucidating the role of dok 3 in b cell receptor signaling using gene knockout mice
... regulators of B cell receptor signaling 14 1.4.2 SH2-containing inositol 5’-phosphatase-1 1.4.4 C-terminal Src tyrosine kinase (Csk) 23 1.4.5 Downstream of tyrosine kinases (Dok) 23 ii Trang ... Mammalian cell culture methodology 60 2.4.3 Purification of bone marrow cells 61 iii Trang 52.5.2 Cloning of targeting construct 64 2.5.3 Culturing of mouse embryonic fibroblasts 64 2.5.5 ... Biological Resource Centre who had help in the microinjection of Dok-3 ES cells, maintaining and changing the many cages of mice, and the staffs from DNA Sequencing Facility for their efforts in
Ngày tải lên: 14/09/2015, 10:44
Exploration of the functional significance of mig 2 in human cancer cell susceptibility to cytotoxic agents and cell growth control a pilot study
... 51.2.1.2 Genetic factors in anticancer drug resistance 61.2.2 Possible role of mig-2 in cell growth control 11 Trang 41.2.2.2 Mig-2 involved in mitogenic signaling cell cycle control 121.3.2 Integrin ... re-expression of mig-2 in human cancer cell lines by transfection to determine thefunction of mig-2 in regulation of cell proliferation; and 4 down-regulation of mig-2 in cancer cell lines by antisense ... cellular localization B Re-expression of mig-2 in mig-2-null cell line HT29 to determine its capability of conferring cell sensitivity to anticancer drugs and to observe the effect of mig-2 on cellbehavior
Ngày tải lên: 05/10/2015, 22:15
The role of prion protein in breast cancer cell metabolism
... M., WIN, T., HAIM, S. B. & ELL, P. J. 2007. Non-[18F]FDG PET in clinical oncology. Lancet Oncol, 8, 822-30. GUERTIN, D. A. & SABATINI, D. M. 2007. Defining the role of mTOR in cancer. Cancer Cell, ... c-Jun-N-terminal kinase (Crozet et al., 2008). Overexpression of PrP in human embryonic kidney 293 cell lines, rabbit epithelial Rov9 cell lines, and murine cortical TSM1 cell line resulted in cells ... Meslin et al., 2007b, Roucou et al., 2005, Yu et al., 2012). The role of PrP in MCF7 in inhibiting TNF (Diarra-Mehrpour et al., 2004) or Bax induced cell death (Roucou et al., 2005) was explained
Ngày tải lên: 12/10/2015, 17:36
The role of caspase 1 in a murine model of influenza pneumonitis, and studies on cell death inhibitors in vitro
... Summaries of experimental procedures 54 2.29.1 Influenza A infection of RAW264.7 murine macrophage cell line in vitro 54 2.29.2 An investigation of the role of caspase-1 in an in vivo mouse model of ... 41 2.18 Histopathological Analyses 43 2.19 Bio-Rad DC Protein Assay 44 2.20 Western immunoblotting 44 2.21 TUNEL Assay 46 2.22 Bio-Plex ProTM cytokine assay 47 2.23 Infection of cell lines ... to infection in vivo 18 1.11 Generation of caspase-1 knock-out mice by germ line gene-targeting 19 1.12 Objectives of study 22 CHAPTER 2 23 2 METHODS AND MATERIALS 24 2.1 Cell lines 24 2.2
Ngày tải lên: 13/10/2015, 16:41
Elucidation of the physiologic role of TRIP br2 in cell cycle regulation and cancer pathogenesis
... Figure 32 H&E staining of liver, heart and kidney of 8-week-old 129 SvEvBrdTRIP-Br2 wildtype and mutant mice 22 8 Figure 33 H&E staining of colon, small intestines and lung of 8-week-old 129 SvEvBrd-TRIP-Br2 ... knockdown of TRIP-Br2 is sufficient to inhibit cell- autonomous growth of HCT-116 colorectal cancer cells in vitro Since the loss -of- and gain -of- function of TRIP-Br2 are associated with cell proliferative ... 122 3 .2. 2 TRIP-Br2 overexpression confers anchorage-independent growth in soft agar and promotes tumor growth in athymic nude mice 129 3 .2. 3 TRIP-Br2 is overexpressed in many human cancer cell...
Ngày tải lên: 13/09/2015, 20:18
Role of bcl 2 in metabolic and redox regulation via its effects on cytochrome c oxidase and mitochondrial functions in tumor cells
... conformational change in Bcl -2, exposing its BH3 domain, converting Bcl -2 from anti-apoptotic to pro-apoptotic (Lin, Kolluri et al 20 04) 1.6 Non-canonical role of Bcl -2 in redox regulation: Just as ... species of free oxygen radicals is important in determining the decision of the cell fate A tilt in favor of O2- levels such as the inhibition of Cu/Zn SOD using DDC, with no appreciable increase in ... reaching detrimental levels In our system, we reason that the involvement of SOD in mediating the maintenance of mitochondrial O2- in CEM/Bcl -2 cells during antimycin treatment is at best, minimal...
Ngày tải lên: 14/09/2015, 08:42
Tài liệu Báo cáo khoa học: A role of miR-27 in the regulation of adipogenesis ppt
... were cultured in DMEM containing 10% fetal bovine serum OP9 cells were grown in aMEM containing 20 % fetal bovine serum C2C 12 cells were maintained in DMEM containing 10% fetal bovine serum Adipogenic ... biologically relevant role of miR -27 in the regulation of adipose tissue functions in vivo, we examined the expression of miR -27 in the genetically obese ob ⁄ ob mice The 23 52 expression levels of ... microscopically with or without hematoxylin staining In hypoxia experiments, 3T3-L1 cells were maintained in a hypoxia chamber (Invivo 400; Ruskinn Inc., Cincinnati, OH, USA) constantly maintained...
Ngày tải lên: 18/02/2014, 08:20
Báo cáo khoa học: Alternative initiation of transcription of the humanpresenilin 1gene in SH-SY5Y and SK-N-SH cells The role of Ets factors in the regulation ofpresenilin 1 pptx
... D’Adamio, L 22 23 24 25 26 27 28 29 30 31 32 33 34 35 (20 00) Generation of an apoptotic intracellular peptide by csecretase cleavage of Alzheimer’s amyloid b protein precursor J Alzheimers Dis 2, 28 9–301 ... a major role in determining both the level of expression and the location of the start of transcription of PS1 In the Ets family of transcription Ó FEBS 20 04 Regulation of the presenilin gene ... containing the Drosophila Elk1 binding motif (25 ) (+) or E74 containing a mutated Ets consensus (m) The position of complexes defined in (A) is indicated by arrows or lines Ó FEBS 20 04 Regulation...
Ngày tải lên: 16/03/2014, 18:20
The role of p38 MAPK in cell cycle checkpoint control following DNA damage
... for cancer Author: Mark Phong Page of 29 1 List of Tables, Figures & Symbols List of Tables S/No Table ID 2. 1 2. 2 2. 3 2. 4 2. 5 2. 6 2. 7 2. 8 2. 9 10 2. 10 11 4.1 12 4 .2 13 4.3 Table 4.3: Genes induced ... Figure 5.9: Effect of Adriamycin and siRNAs Figure 6.1: A new model of p38’s role in DNA damage response 22 0 22 1 22 3 22 6 24 1 Page 13 of 29 1 List of Tables, Figures & Symbols LIST OF SYMBOLS & ABBREVIATIONS ... of Signal Transduction in mammalian cells Figure 1 .2: Canonical overview of the MAPK signaling cascade Figure 1.3: Canonical p38 MAPK signaling pathway: Receptors and signaling cascades leading...
Ngày tải lên: 11/09/2015, 09:02