regulation of sex determination in drosophila melanogaster

... protein kinase; LY-AMC, N-succinyl-Leu-Tyr-7-amino-4-methyl-coumarin; MAP, microtubule-associated protein; Ni-NTA, nickel–nitrilotriacetic acid; PKA, protein kinase A. Trang 2The regulation of intracellular ... signaling pathways involves an intricate interplay of various enzyme sys-tems One intriguing example is the interaction between calpains (i.e the Ca2+-dependent SH-prote-ases) and protein kinases ... subunits of mammalian l- and m-cal-pain They are composed of an N-terminal regulatory domain (I), a catalytic domain (II), a C2-like domain (III) and a calmodulin-like calcium binding domain (IV)...

Ngày tải lên: 16/03/2014, 02:20

... Attenuation of insulin signaling in cullin-1 mutant does not affect normal dendrite elaboration 86 3.7.5.2 Attenuation of insulin signaling in cullin-1 mutant promotes proximal severing of major ... Attenuation of insulin signaling in cullin-1 mutant promotes proximal severing of major dendrites 88 Figure 22: Attenuation of insulin signaling does not rescue dendrite pruning defect in mical ... secreted from winning axon binds to p75NTR on the losing axon, resulting in axon pruning of the latter Mechanistically, binding of BDNF to p75NTR results in suppression of TrkA-mediated signaling which...

Ngày tải lên: 09/09/2015, 11:16

... expressed in the brains than in the testis, forming the brain and/or influencing the sexual development gene network via the action of hormones Their expression can be maintained (continuous line) ... estrogen sex-reversed fish Sexually undifferentiated fry including both, XX- and with a sex ratio of 1:1, with the corresponding female XX and male XY genotypes in the offspring Crossing of sex-reversed ... [62] Interestingly, ar is higher expressed in brains of dominant A burtoni males than in subor-dinate males [63] In the developing gonads of the Nile tilapia the expression levels of ar in males...

Ngày tải lên: 27/03/2023, 04:27

... members of the basal targeting machinery (Mira and PON) or in cell fate determination (Numb, Pros and Brat) impair terminal differentiation of GMCs Trang 39Figure 7 Models of the origin of Drosophila ... through direct protein-protein interaction Par-6 is a small protein that contains a PDZ domain, a PB1 domain and an atypical CRIB domain Par6 binds aPKC through the PB1 domain, and binds Rho GTPase ... during the committee meetings My sincere gratitude goes to Liu Ming for being a friend, and for his very kind assistance with microinjection for the generation of some of the zif transgenic lines...

Ngày tải lên: 11/09/2015, 14:32

... germline specific isoform of regulatory b subunit of casein kinase 2 Western-analysis using anti-CK2btes Ig revealed CK2btes protein in Drosophila testes extract Expression of a CK2btes– b-galactosidase ... calculated in Miller units according to the formula: units¼ 1000 · A420/(culture volume in ml· incubation time in min· D600) In addition, the ability of the yeast strain HF7c (carrying HIS3reporter ... CK2btes protein 2.5-fold Fig 1 Expression of the CK2btes protein in testes (A) Detection of CK2btes protein in Drosophila tissues Western analysis using poly-clonal anti-CK2btes Ig: R, recombinant CK2btes...

Ngày tải lên: 21/02/2014, 15:20

... understand the roles of the epoxide hydrolases (EHs) in xenobiotic biotransformation in insects, we exam-ined the induction of EHs by exogenous compounds in Drosophila melangaster third instar larvae ... 463 amino acids in an open reading frame Northern blot analysis showed that the transcription of DmEH was increased in larvae within 5 h of clofibrate treatment Recombinant DmEH expressed in baculovirus ... three proteins; dots indicate amino acids identical between two proteins The small box indicates the position of substituted amino acids within the mEH catalytic triad (Glu in DmEH; Asp in Jheh1...

Ngày tải lên: 07/03/2014, 21:20

... were grown overnight in 10 mL Luria–Bertani medium containing kanamycin and chloramphenicol (also containing ampicillin in the case of double transformation) In the case of trial expression, ... expression of proteins of
50 kDa, whereas in the case of the different forms of GER the proteins were
35 kDa (Fig 3) The Caenor-habditis and Drosophila GMD and GER proteins were tested for activity in ... reducing the NADPH concentration by half, or increasing it twofold, had no influence on the yield of GDP-Fuc Furthermore, reduced conversion of the intermediate was observed in the absence of dithiothreitol...

Ngày tải lên: 16/03/2014, 13:20

... surface bind-ing sites for peanut agglutinin in the differentiatbind-ing eye disc of Drosophila Dev Biol 92, 418–427 8 D’Amico P & Jacobs JR (1995) Lectin histochemistry of the Drosophila ... stress often lead to lineage-specific expansion of gene clusters involved in such responses [23] In this context, the specific expansion of core-1 b3GalT genes in D mela-nogastermay be interpreted in ... Gal(b1-3)GalNAc-bind-ing lectin peanut agglutinin failed to reveal any signal in embryonic salivary glands [8], which could mean that salivary O-glycan chains are elongated, thus abro-gating peanut agglutinin...

Ngày tải lên: 23/03/2014, 15:20

... three types of models were constructed corresponding to these factors In the Trang 6analyses of the genetic background, pooled according originof the strains (i.e isofemale lines) Hence, in these ... three isofemale lines differed significantly in their initial survival rates in both life stages (table I: IFL) The strains originating from isofemale line A (figure 1) had lower survival in the ... striking as six strains with Adh genotype (originating from three different isofemale lines) and four strains with Adh genotype (originating from two isofemale lines) were analysed in this study (figure...

Ngày tải lên: 09/08/2014, 18:21

... acetic-acid tolerance in Indian populations of Drosophila melanogaster could be explained by balancing natural selection varying spatially along the north-south axis of the Indian subcontinent ADH polymorphism ... objective of this study is to analyse acetic-acid and ethanol utilisation by D melanogaster populations from the Indian subcontinent MATERIALS AND METHODS Isofemale lines of D melanogaster from 9 Indian ... l’acide acétique / clines de latitude / populations indiennes / Drosophila melanogaster INTRODUCTION The evolutionary potential of a species is a function of the amount of genetic vari-ation...

Ngày tải lên: 09/08/2014, 18:21

... system inDrosophila melanogaster involving expression of a quantitative mutant phenotype,radius incompletus (interruption of radial wing vein) Below we present a short review of our protracted investigations ... to78C The mutant phenotype consists of an interruption in the radial wing vein (L2), producing distal and proximal fragments ; the remaining lenghts of wing vein giving a quantitative character which ... the arrows indicate the generation of total restoration of the radial vein Atypical phenotype of invididuals is shown in figure 2 (c) ; table 1 contains statistical parameters of a line in the 40th...

Ngày tải lên: 09/08/2014, 22:22

... protein bearing the N2 domain than in the protein bearing the Nc domain Thus, the accessibility of the active site in the N2 proteins was independent of the nature of the C-terminal domain, in contrast ... protein The chimeric protein composed of the N-terminal domain of hoPL and of the C-terminal domain of hoPLRP2 was named NcC2 Conversely, the other chimeric protein, bearing the N-terminal domain ... the N-terminal and C-terminal domains of hoPL were involved in the inactivation of the protein at the water–triglyceride interface The inactivation of N2Cc in the absence of bile salts was prevented...

Ngày tải lên: 16/03/2014, 06:20

... organ of the marine ray, Torpedo californica [3] The acquisition of the crystallographic structure of the mollusc acetylcholine-binding protein [11] has provided an insight into the ligandbinding ... ligand-binding extracellular domain of the human alpha7 acetylcholine receptor expressed in yeast: replacement of the hydrophobic cysteine loop by the hydrophilic loop of the ACh-binding protein enhances ... cleavage of the signal peptide) consists of three domains, an extracellular domain (ECD) (210–220 residues), a membrane-spanning domain, and an intracellular domain [3] The N-terminal ECD of each of...

Ngày tải lên: 30/03/2014, 10:20

... epitopes of gp130 are involved in ligand binding: the Ig-like domain and the CBM [9–11] In the case of LIF-induced heterodimerization of LIFR with gp130, the cytokine first binds to the Ig-like domain ... epitopes involved in ligand binding are unchanged Replacement of D6 of gp130 by the corresponding domain of the GCSFR leads to the abrogation of signal transduction of all tested cytokines As ... tyrosine motifs of gp130 that predominantly recruit STAT3 were replaced by the STAT1 recruiting motif of the interferon-c receptor resulting in a chimeric protein designated gp130c In order to investigate...

Ngày tải lên: 18/03/2014, 01:20

... purification of an AnfA variant reported by Austin et al [22] In their study, the N-terminal domain of AnfA was truncated to prevent the intrinsic aggregation of the intact form during purification ... absence of the N-terminal domain in this variant Because sensing is a principal function of regulatory proteins, the isolation of VnfA and AnfA with their sensor (GAF) domains is highly desirable In ... effect of the nucleotide binding was reported in a study of the limited trypsin digestion with NifA + MgADP, in which binding MgADP to the central AAA+ domain is ascribed to the trigger of a conformational...

Ngày tải lên: 15/03/2014, 09:20

... bioconversion of chitin into valuable oligosaccharide derivatives for various industrial applications Pf-ChiA has a unique multidomain structure containing two chitin-binding domains (ChBD1 and ... number of amino acid residues contribute to the binding of the substrate by hydrogen bonding and ⁄ or hydrophobic interactions Using the ligplot program [31], we investigated the specific interactions ... degree of sequence similarity The catalytic domain of family 19 chitinases comprises two lobes, each of which is rich in a-helical structure [16,17] In contrast, family 18 includes chitinases...

Ngày tải lên: 15/03/2014, 11:20

... domain–domain interactions are relieved by binding of the metal to the N-terminus of the ATPase To further study the role of the Ccc2 N-terminus in the overall function of the ATPase in vivo, we engineered ... distinct roles of the N-terminal copper-binding sites in regulation of catalytic activity of the Wilson’s disease protein J Biol Chem 278, 32212–32218 40 Bal N, Wu CC, Catty P, Guillain F & Mintz ... cysteines are involved in binding Cu(I) [12,13] Ccc2 and the mammalian copper ATPases all possess a cytosolic N-terminus made of between two and six metal-binding domains (MBD) and each of these...

Ngày tải lên: 23/03/2014, 05:22

... additional N-domain, homologous to the N-domains of ClpA or ClpB, and a linker domain homologous to, but half the size of, the linker domain of ClpB [19] The N-terminal region contains two 32-amino acid ... activity in preventing the aggregation of luciferase and reactivating heat-inactivated luciferase Deletion of the N-terminal conserved repeat I (amino acids 1–38) resulted in an alteration in the ... caused by deletion of amino acids 1–38 rendered ClpC1D1 prone to heat denaturation Deletion of N-terminal conserved repeat I along with the intervening amino acids linking it to N-terminal conserved...

Ngày tải lên: 23/03/2014, 06:20

... Diagram 1of the HRSV F protein Diagram of the HRSV F protein A linear representation of the HRSV F precursor protein (A2 strain) is shown Amino acid positions of individual domains are indicated ... These findings are intriguing given that these three cysteine residues are absolutely conserved not only in the F proteins of other Pneumovirinae, but also in the F proteins of the Paramyxovirinae ... reduced binding of all four antibodies, while mutation of cysteine mutants C382S, and C422S retained similar levels of antibody binding as the wildtype F protein (Table 1) As the same set of cysteine...

Ngày tải lên: 20/06/2014, 01:20

... by addition of uninfected cells and culture medium Lysates of equal numbers of cells, now containing equal percentages of infected cells, were prepared, and protein determination using standard ... (

Ngày tải lên: 12/08/2014, 23:23