regulation of peripheral inflammation by spinal adenosine

... 1REGULATION OF RHOGAP DLC1 BY FAK, PP2A AND MEK/ERK IN CELL DYNAMICS ARCHNA RAVI (M.Sc., University of Madras, India) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ... RhoGAP, one of the p190RhoGAP isoforms affects cell growth and differentiation, an effect seen by the reduction in size of mice thymus in the absence of p190B MgcRacGAP mediated regulation of Cdc42 ... other controlled by RhoGTPase activity G1-S progression depends on the regulation of cyclin and Cdk inhibitors Cyclin concentrations are affected by maintaining the levels of ERK and by extracellular

Ngày tải lên: 10/09/2015, 09:29

... neuromodulator of the N-methyl-Daspartate (NMDA) receptors The aim of this thesis is to examine the modulation of NR2B-containing NMDA receptors by the tPA/plasminogen system -Through the analysis of tPA-treated ... mechanisms engaged by tPA in the modulation of NMDA receptors would enable us to have a better understanding of the multi-faceted roles of tPA in the brain (500 words) Trang 10List of tables Table ... 11List of figures Figure 1.1 Classification of glutamate receptors 2 Figure 1.2 The different isoforms of the NR1 subunit 5 Figure 1.3 The tetrameric NMDA receptor structure and the topology of a

Ngày tải lên: 11/09/2015, 10:18

... Trang 1REGULATION OF GENE EXPRESSION BY ESRRB IN EMBRYONIC STEM CELLS ZHANG WEIWEI NATIONAL UNIVERSITY OF SINGAPORE 2008 Trang 2REGULATION OF GENE EXPRESSION BY ESRRB IN EMBRYONIC ... group of Genome Institute of Singapore), Eleanor Wong (Cloning and Sequencing group of Genome Institute of Singapore), Han Xu (Information & Mathematical Sciences Group of Genome Institute of ... regulatory loop formed by Esrrb, Nanog and Oct4 141 Figure 4.2 The regulation of differentiation-associated genes by Esrrb 144 Figure 5.1 Model for the role of Esrrb in gene regulation in pluripotent

Ngày tải lên: 12/09/2015, 08:19

... 151 4.1 Control of floral patterning by flowering time genes 152 4.2 Transcriptional activation of class B and C genes by SEP3 and LFY 153 4.3 Regulation of SEP3 expression by SOC1, AGL24, and ... 7partner of SOC1 and AGL24; it is one of the core components of histone deacetylation complex Further studies have shown that the repression of SEP3 by SOC1 and AGL24 is mediated by deacetylation of ... initiation of floral meristems The initiation of FMs from IMs starts with the onset of FM protrusion and acquisition of cell polarity Regulation of such a process not only involves the activation of

Ngày tải lên: 14/09/2015, 08:47

... and Future Work 6.1 Regulation of Spindle Dynamics by DNA Replication Checkpoint 183 6.2 Regulation of Spindle Dynamics by the DNA Damage Checkpoint 186 6.3 A Unified View of DNA Replication ... Discussion 114 CHAPTER 4 Regulation of Spindle Dynamics by DNA Damage Checkpoint 4.1 Introduction 120 4.2 Artificial removal of Cohesin does not Lead to Complete Segregation of Damaged Chromosomes ... (B.Tech.(Hons), Indian Institute of Technology, Kharagpur) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY INSTITUTE OF MOLECULAR AND CELL BIOLOGY NATIONAL UNIVERSITY OF SINGAPORE 2009 Trang

Ngày tải lên: 14/09/2015, 14:03

... Trang 1THE ROLE OF DMSO IN THE REGULATION OF IMMUNE RESPONSES BY DENDRITIC CELLS ELAINE LAI MIN CHERN Trang 2I would like to express my heartfelt gratitude to Assoc Prof Lu Jinhua for his ... IL-12 and down-regulation of IL-10 production upon IFN-γ/LPS stimulation IL-23 production by DCs was also up-regulated by DMSO priming Macrophage displayed a more tolerogenic profile and was not ... weak production of pro-inflammatory cytokines The identification of the diverse macrophage populations found in various organs of the human body was made possible by the use of the antigenic

Ngày tải lên: 16/10/2015, 11:59

... knockdown of NF-κB subunits and the downregulation of Mcl-1 expression, we next tested the effect of the NF-κB subunit siRNAs on TE-1 cell viability Silencing of p50 or p65 resulted in decrease of Mcl-1 ... promoter in intact cells by ChIP assay, respect-ively Our data provided evidence that one of the regulatory mechanisms by which Mcl-1 expression in hu-man ESCC is by binding of p50 and p65 toκB site ... Re-presentative time-course data of Mcl-1 reduced by p50 or p65 siRNA was shown in Figure 6A and B The levels of endogenous p50 and p65 decreased by 24 h after transfec-tion of si-p50 or si-p65 and peaked

Ngày tải lên: 05/11/2020, 01:14

... suppressor of inflammation, oxidative stress and apoptosis from cigarette smoke by mechanisms that may involve the regulation of microRNA However, little is known about the AhR regulation of miRNA ... translation of the transcript; both of these result in inhibition of protein production18 Since the elucidation of the role of the miRNA lin-14 and lin-4 in the developmental timing of C Elegans, ... suppressor of inflam-mation, oxidative stress and apoptosis caused by exposure to cigarette smoke, the leading cause of preventable death worldwide8–13 Many of the protective functions of the AhR

Ngày tải lên: 19/11/2022, 11:45

... function of Bdf2 To assess whether loss of Leo1 might instead affect recruitment of the H4K16 acetyltransferase Mst1 to the IRC, we analysed association of Mst1 with IRC chromatin by ChIP Levels of ... acetylation by Mst1, or increased deacetylation owing to decreased binding of Bdf2 To investigate whether loss of Leo1 affects binding of Bdf2 at the IRC, we analysed association of Bdf2 with ... Moreover, a side-by-side comparison revealed that loss of either Leo1 or Epe1 results in much stronger silencing of that spreading of heterochromatin in leo1D cells cannot be explained simply by a defect

Ngày tải lên: 04/12/2022, 10:35

... characteristic of sleep has obvious fitness costs in an environment containing predators and a limited supply of food and mates Thus, the timing of sleep must be tightly regulated by an array of sensory ... homeostatic regulation of sleep7–14, as well as its neurobiological functions3–5,15 Drosophila exhibits a daytime siesta and long bouts of consolidated sleep during the night Components of this ... development of a subset of clock cells termed DN1p neurons26,28, providing a possible explanation for this discrepancy (see below) Interestingly, we also found that PMW was suppressed by loss of CRYPTOCHROME

Ngày tải lên: 04/12/2022, 16:03

... for the Use of Experimental Animals of Ocean University of China All animal care and use procedures were approved by the Institutional Animal Care and Use Committee of Ocean University of China ... targeting FADS2 1Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, ... a total volume of 25 ml, containing 1 μ l of each primer (10 mM), 1 μ l of the diluted first strand cDNA product, 12.5 μ l of 2 × SYBR Premix Ex Taq II (TaKaRa Bio) and 9.5 μ l of sterilized double-distilled

Ngày tải lên: 04/12/2022, 16:13

... allow for effective loss-of-function of miRNAs and consequent gain-loss-of-function of their target genes To achieve concomitant knockdown of multiple miRNAs, co-application of multiple singular ... control; n = 3 (D) Down-regulation of MAPK 14 protein expression lev-els by miR-27a mimic in L6 cells * p < 0.01 vs control; n = 3 (E) Upregulation of MAPK 14 protein levlev-els by AMO-27a to knockdown ... control; n = 3 (D) Down-regulation of PI3K regulatory subunit beta protein expression levels by miR-30d mimic in L6 cells ** p < 0.01 vs control; n = 3 (E) Upregulation of PI3K regulatory subunit

Ngày tải lên: 04/12/2022, 16:13

... transcript levels of the candidate genes The up or down regulated GSTs were grouped by Hierarchical clustering with the MeV 4.0 software GO annotation of Tau MeGSTs genes was carried out by BiNGO according ... levels of proline and antioxidant can enhance resistance to stress tolerance in cassava We also discov-ered high levels of proline in cassava induced by drought cassava by regulating the levels of ... GSTs can be induced by allyl isothiocyanate at lower doses; the up-regulation of GSTs was proved to promote the tolerance to oxidative stress [17] In rice, over expression of GSTU4 has been proved

Ngày tải lên: 30/01/2023, 20:00

... cellular level of NADH and extension of lifespan However, this theory has been challenged by reports that regulation of Sir2p activity by nicotinamide, the noncompetitive inhibitor of Sir2 proteins, ... This was further supported by the ability of NADH to act as a competitive inhibitor of Sir2p and by manipulating intracellular levels of NADH through the overexpression of NADH-reductase, which ... cell cycle 26 The nicotinamide binding pocket of Hst3 is necessary for regulation of The phenotypes of hst3 hst4 mutant cells are caused by high levels of The histone chaperone Asf1p is needed

Ngày tải lên: 02/10/2024, 01:44

... will be made available soon Modulation of spinal cord synaptic activity by tumor necrosis factor alpha in a model of peripheral neuropathy Journal of Neuroinflammation 2011, 8:177 doi:10.1186/1742-2094-8-177 ... cited Modulation of spinal cord synaptic activity by tumor necrosis factor α in a model of peripheral neuropathy Diana Spicarova, Vladimir Nerandzic and Jiri Palecek Department of Functional Morphology, ... Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic Authors: Diana Spicarova, Ph.D Department of Functional Morphology, Institute of Physiology vvi, Academy of

Ngày tải lên: 19/06/2014, 22:20

... Pathogenesis of IBD 2! 1.1.2.! Dextran Sulphate Sodium (DSS)-induced Murine Model of Colitis 5! 1.2.! The Role of Immune Response in Intestinal Inflammation 5! 1.3.! The Role of Intestinal ... to investigate the role of MKP-3 in intestinal inflammation The results showed that overexpression of MKP-3 downregulated the phosphorylation of all three major groups of MAPKs (ERK, JNK and p38) ... colon of MKP-3-/- mice In addition, Ki67 staining showed increased IEC proliferation in the colon of MKP-3-/- mice These results suggested the role of MKP-3 in IEC during intestinal inflammation by...

Ngày tải lên: 01/10/2015, 17:28

... polyamine in the regulation of apoptosis [21,22] Thus, the possibility that the antiviral effect of spermine is mediated by induction of apoptosis was analyzed For this purpose, the effect of spermine ... pathway of translational regulation mediated by eukaryotic initiation factor EMBO J 19, 6891–6899 Geiss G, Jin G, Guo J, Bumgarner R, Katze MG & Sen GC (2001) A comprehensive view of regulation of ... line following inducible expression of the constitutively active form of IRF-3, IRF-3 5D [14] Up -regulation of ArgII gene expression was observed after treatment of the tetracycline inducible cell...

Ngày tải lên: 07/03/2014, 21:20

... on loans on the basis of market capital supply – demand and the creditability of borrowers” Page of 20 International Finance Regulation of Interest Rate by the State Bank of Vietnam (SBV) This ... Liberalization of interest rate From the year Page 18 of 20 International Finance Regulation of Interest Rate by the State Bank of Vietnam (SBV) 1994, Credit limit mechanism was deleted, replacing by other ... Loc MBA Intake 13 CFVG, Hanoi, June 2006 Page of 20 International Finance Regulation of Interest Rate by the State Bank of Vietnam (SBV) THE ROLES OF INTEREST RATE Interest Rate is a key economic...

Ngày tải lên: 15/03/2014, 01:20

... satisfied Brian P Ceresa, Ph.D Department of Pharmacology University of Louisville, Louisville, Kentucky, USA Section Regulation of Tissue Homeostasis by the Endocytic Pathway Chapter Key Events ... heavy chain, Molecular Regulation of Endocytosis illumination followed by repetitive stimulation causes a complete loss of synaptic vesicles along with massive accumulation of plasma membrane folds ... has been determined by taking advantage of assembly-deficient mutants (66,67) Insight into the organization of assembled dynamin multimers has been gained by computer docking of domain crystal...

Ngày tải lên: 24/03/2014, 02:21

... [57,58], Ga ⁄ tubulin Agonist GPCR Gαβγ α Gα Activation of GTPase of tubulin by Gα, and inhibition of MT assembly Gβγ Loss of GTP cap on MT end by Gα promotes catastrophy in di and increase i MT dynamics ... Effect GDPTubulin Effectors Promotion of MT assembly by Gβγ GTP-Tubulin γ-Tub Fig Model for the regulation of microtubule (MT) assembly by a and bc subunits of G proteins Based on in vitro results ... bound form of Gia and inhibit the exchange of GDP-bound for GTP-bound Ga [73–75] These signaling partners of G proteins might also be involved in the regulation of microtubule assembly by Gia or...

Ngày tải lên: 30/03/2014, 10:20