redox regulation of gene expression and cell function

... of a native redox process to actuate gene expression This bacterial electro-genetic device is simple, specific and versatile We take advantage of the well-characterized native redox-response of ... Additionally, we expand on this genetic circuit by demonstrating electronic induction of cell motility and by connecting electronically actuated cells to non-actuated cells via generation of the native ... cells and gene expression Figure 1a provides a schematic representation of our approach To test the effect of pyocyanin, Fcn(O), and Fcn(R), on gene expression and their interactions with cells we

Ngày tải lên: 24/11/2022, 17:42

... UTILIZATION OF A CANINE CANCER CELL LINE (FACC) PANEL IN COMPARATIVE AND TRANSLATIONAL STUDIES OF GENE EXPRESSION AND DRUG SENSITIVITY Submitted by Jared S Fowles Graduate Degree Program in Cell and ... Oncology of Melanoma Epidemiology of human and canine melanoma 22 Comparative biology of melanoma 23 Comparative genetics and molecular biology of melanoma 25 Treatment of human and canine ... Combined inhibition of MAPK and PI3K/AKT pathways is synergistic in human and canine melanoma cells 166 Figure 3.7 Cell cycle analysis of human and canine melanoma cells after AZD6244 and/or rapamycin

Ngày tải lên: 07/08/2019, 02:33

... expression of stress-response genes is regulated Transcription factors play an important role in regulating the expression of the stress-response genes Another level of regulation of gene expression ... effects on the growth and development of plants (Abdeen et al 2010) Post-transcriptional regulation of gene expression Besides stress-induced regulation of gene expression at the tran-scription level, ... downregulated the expression of a histone deacetylase AtHD2C, while overexpression of this enzyme brought about enhanced expression of ABA-responsive genes and greater salt and drought tolerance

Ngày tải lên: 07/10/2021, 01:20

... Trang 1REGULATION OF GENE EXPRESSION BY ESRRB IN EMBRYONIC STEM CELLS ZHANG WEIWEI NATIONAL UNIVERSITY OF SINGAPORE 2008 Trang 2REGULATION OF GENE EXPRESSION BY ESRRB IN EMBRYONIC STEM CELLS ... the study of ES cell biology First of all, mouse ES cells provide a universal cell source for the study of embryogenesis, gene function and directed differentiation Because mouse ES cells are ... understanding of the roles of extracellular and intracellular signals in the maintenance of mouse ES cells holds promises to generate improved methodologies for maintenance and proliferation of human

Ngày tải lên: 12/09/2015, 08:19

... operons.  How positive and negative control function in gene expression.  The impact of DNA methylation and histone acetylation on gene expression.  How timing and coordination of specific events ... formation and induction.  The role of miRNAs in control of cellular functions.  The role of gene regulation in embryonic development and cancer. Trang 7TranscriptionTrang 9Operon: cluster of related ... product of proto-oncogene  Increase activity of each protein molecule produced by gene Trang 51 Ras gene: stimulates cell cycle (proto-oncogene) ◦ Mutations of ras occurs in 30% of cancers ◦ Functions:

Ngày tải lên: 18/05/2017, 08:54

... Trang 1Lecture Connections28 | Regulation of Gene Expression © 2009 W H Freeman and Company Trang 2CHAPTER 28Regulation of Gene Expression – DNA elements that control transcription ... stimulates expression of the lac operon Trang 37CRP Homodimer Binds and Bends DNA polymerase, stimulating transcription of genes in the lac operon Trang 39Combined Effects of Glucose and Lactose ... protein’s activity in the cell • Regulation of transcription is commonly achieved via binding of inhibiting and activating proteins to the DNA near the beginning of the gene • Transcription factors

Ngày tải lên: 15/06/2017, 20:50

... TỐ PHIÊN MÃ Trang 7Quorum sensingTrang 8Regulation of Porin Proteins by a Two Component Signal Transduction SystemTrang 9POSITIVE REGULATION OF GENE EXPRESSION – ĐIỀU HÒA DƯƠNG BIỂU HIỆN GEN“Bật” ... hiện ở operon lacTrang 18The lac operon allows the option of lactose utilization as a carbon source Trang 19NEGATIVE REGULATION OF GENE EXPRESSION – ĐIỀU HÒA ÂM BIỂU HIỆN GEN“Tắt” biểu hiện gen ... Trang 1ĐIỀU HÒA BIỂU HIỆN GEN – REGULATION OF GENE EXPRESSIONTrang 3CÁC VẤN ĐỀ CẦN TÌM HIỂU1 Điều hòa biểu hiện gen ở prokaryote 2 Điều hòa

Ngày tải lên: 09/09/2019, 10:52

... that a total of 965, 1662 and 3783 genes showed significantly up-regulated expression and a total of 287, 1511 and 3261 genes showed significantly down-regulated expression by P, HC and PHC treatments, ... alteration in expression of 12 selected DEGs identified by cDNA microarray and profile the expression dynamics of these genes during the 48 h of PHC treatment usingeIF4A as a reference gene as its expression ... accumulation of excessive ROS, and the expression of alternative oxi-dase gene (AOX1a) is also known to respond to various stresses in plants [58] The expression ofAOX1 affects both ROS and RNS generation

Ngày tải lên: 22/05/2020, 04:44

... Activation of mARs eventually leads to modification of tumor cell proliferation, migration and apoptosis [5, 6, 13, 14, 19]. Cell proliferation, migration and cell death are regu-lated by alterations of ... understand the complex signaling of mAR induced cel-lular functions and death [46]. Conclusions In conclusion, transient up-regulation of Orai1 protein abundance and transient increase of store ... important for rapid androgeneffects [31]. The present study explored, whether mAR activation is followed by alterations of Orai1 protein abundance and function in MCF-7 breast tumor cells and addressed

Ngày tải lên: 22/09/2020, 23:09

... RNAi screening and next generation sequencing of cell lines and primary tumor material will promote the identification and functional characterization of non-coding RNAs, RBP, and regulatory ... posttranscriptional control of gene expression Anderson and colleagues character-ized the MK2-mediated regulation of the zinc finger protein Tristetraprolin (TTP), which had been shown to bind and desta-bilize ... the context of active cell cycle checkpoints We have hence used live cell imaging to follow the subcellular dis-tribution of Cdc25B and C after genotoxic stress in control cells or cells that

Ngày tải lên: 04/12/2022, 16:01

... 6Secretion of IL-8 and CCL20 and Induction of cell surface ICAM-1 expression in A549 cells cocultured with MT-2 cellsFigure 3 Secretion of IL-8 and CCL20 and Induction of cell surface ICAM-1 expression ... expression of cytokines, chemokines and cell adhesion molecule in A549 cells cocultured with MT-2 cells Figure 2 Induction of expression of cytokines, chemokines and cell adhesion molecule in A549 cells ... specimens of A549 and NCI-H292 cells at 3 and 5 days of culture contained no viable MT-2 cells As shown in Figure 1, A549 and NCI-H292 cells cocultured with MT-2 cells showed strong expression of Tax

Ngày tải lên: 13/08/2014, 05:21

... ROLE OF TRIP-BR PROTEINS IN THE REGULATION OF MAMMALIAN GENE TRANSCRIPTION AND CELL CYCLE PROGRESSION (BSc & ARCS, Imperial Col ege of Science, Technology and Medicine, University of London) ... presence of FBW7 rescue 109 FIGURE 22A: Schematic illustration of the general structural and functional features of a DNA enzyme 111 FIGURE 22B: Post-transcriptional suppression of gene expression ... the cases of Rsc1 and Rsc2) 15 Trang 28(1.2.2) The role of PHD zinc fingers and bromodomains in the regulation of eukaryotic gene transcription To date, the molecular function(s) of the PHD

Ngày tải lên: 16/09/2015, 17:12

... through several downstream target genes [18-21] We investigated the expression of Gata4 and Gata6 and the downstream target gene, Dab2 in Emb-LPD and NPD EBs Gata6 gene expression was signifi-cantly ... H A R T I C L E Open AccessEpigenetic regulation of histone modifications and Gata6 gene expression induced by maternal diet in mouse embryoid bodies in a model of developmental programming Congshan ... EBs of Emb-LPD and NPD groups presented as ratio to the geometric mean of Gapdh and Ppib transcripts (n = 5 per treatment) (B) Expression of Gata6 and Dab2 protein in EBs from NPD and Emb-LPD groups

Ngày tải lên: 02/11/2022, 09:29

... Actin Fig 4 Expression and sub-cellular localization of ASF ⁄ SF2 in normal and arsenite-treated cells (A) NIH3T3 cells were treated for increasing time periods with arsenite (1 m M ) and ASF ⁄ ... different mutants (B, D) Subcellular distribution of ASF ⁄ SF2 mutants in untreated COS cells (B) and in COS cells treated with arsenite (D) Cells were fixed and the localization of the proteins was ... presence of RNAse A in the cell lysate (B) Sub-cellular distribution of TIAR partners COS cells were transfected with the DNA constructs encoding the HA-tagged interact-ing candidates and were

Ngày tải lên: 16/02/2014, 15:20

... promoter region of pRIBOTEX and related vectors induces the transcrip-tion of genes cloned downstream of the promoter and their chromosomal integration [19] After 48 h of transfection, geneticin (G418) ... deletion mutant for ODC and ADCgenes, we used these polyamine auxotrophic par-asites as recipients of foreign ODC or ADC genes to study their expression and the eventual suppression of polyamine auxotrophy ... during the transient expression of the foreign gene After-wards, the free plasmid disappeared almost completely for several weeks and, finally, when the expression of the ADC gene became stable,

Ngày tải lên: 19/02/2014, 07:20

... carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells Amit K Pandey, Vikash Bhardwaj* and Malabika Datta Institute of Genomics and Integrative ... the same In view of these controversial reports, we sought to decipher the role of Foxa2 (HNF-3b), if any, in the regulation of gluconeogenesis in HepG2 cells, and the effects of TNFa pretreatment ... liver-specific functions, and, most importantly, these functions are stable through passages They are therefore valuable in the study of several hepatic functions and other aspects of metabo-lism, and have

Ngày tải lên: 16/03/2014, 02:20

... apop-totic cell death induced by PMA/ionomycin treatment of the DT40 cell line Up- and downregulation of expression of the E2A or Aiolos genes by PMA/ionomycin treatment To determine whether or not expression ... increased expression of the E2A gene and dramatically suppressed that of the Aiolos gene in DT40, but in E2A)/)had no effects or similar effects on the expression of other disrupted genes (Fig 3A) and ... Section of Biochemistry and Molecular Biology, Department of Medical Sciences, Miyazaki Medical College, University of Miyazaki, Japan 2 Section of Surgical Oncology and Regulation of Organ Function,

Ngày tải lên: 16/03/2014, 04:20

... the balance of conven-tional and innate T-cell lineages In the absence of Itk, there is a reduction of con-ventional CD4 + and CD8 + cells and an expansion of innate CD4+and CD8+T-cell lineages ... multiple levels of regulation of cytokine production Itk and the regulation of cytokine-producing innate T-cell populations The contributions of TFKs to peripheral T-cell activa-tion and effector ... or Itk and Rlk⁄ Txk show altered T-cell development and impaired mature T-cell effector function, highlighting the importance of this family in T cells [1] In addition, altered expression of Tec

Ngày tải lên: 28/03/2014, 22:21

... Inhibition of uPAR by DNAzymes all of which contribute to focal proteolysis and regulation of wound healing, angiogenesis, and metastasis [5] Of the components of the uPA system, uPAR is of particular ... control and mutant DNAzyme-treated cells (Fig 5) Inhibition of Saos-2 cell invasion by Dz720 The plasminogen activator system can promote the breakdown of ECM and facilitate cell invasion and metastasis ... substrate, and a maximum reduction of 84% of uPAR substrate concentration at a molar ratio of : 200 (Fig 2D) The specificity of the DNAzymes was confirmed by the lack of cleavage by the untreated and...

Ngày tải lên: 16/03/2014, 22:20

... First, single strands of the promoter sequences of the A thaliana genes were randomized 200 times, the reverse complement of the randomized strand was determined, and the number of times the 33 ... dependent and independent component to carbon and nitrogen regulation Two examples of Model are shown (genes Y and Z) For gene Y, nitrogen only has an effect in the presence of carbon, while for gene ... CN-element gene for an alignment Despite this lack of similarity, we tested for the presence of CN1 and CN2 in the promoter of this gene; three copies of CN1 (p-value = 0.052) and one copy of CN2...

Ngày tải lên: 14/08/2014, 14:21