recent developments in next generation sequencing annotation

... therapies High-throughput sequencing including (1) targeted sequencing (genes of interest), (2) whole exome sequencing (protein-coding portions), and (3) whole genome sequencing (the entire human ... calling, and finally interpretation and reporting Fig 2 Basic scheme of a next generation experiment using an Illumina sequencing platform In clinical NGS, quality control procedures must be incorporated ... is indicative of rapid advancement of the field Third generation sequencing (or next-NGS) was developed with the purpose of making sequencing cheaper than second-generation sequencing Third generation

Ngày tải lên: 14/01/2020, 23:30

... text-mining, and previous knowledge in database In this study, the significantly dysregulated genes were input into STRING for protein-protein interaction network analysis The minimum required interaction ... collected and integrated this information, by consolidating known and predicted protein-protein association data of various organisms (28) The protein-protein interactions, including direct (physical) ... was directly determined using Illumina's sequencing-by-synthesis (SBS) technology Sequencing data (FASTQ files) were generated by Welgene's pipeline based on Illumina's base-calling program bcl2fastq

Ngày tải lên: 15/01/2020, 10:05

... Urothelial Carcinoma Using Next-Generation Sequencing and Bioinformatics Approaches Hsiang-Ying Lee1,2,3*, Yi-Jen Chen1,4*, Ching-Chia Li2,3,5,6, Wei-Ming Li3,5,6,7, Ya-Ling Hsu6, Hsin-Chih Yeh2,3,5,6, ... urothelial carcinoma; cell cycle; matrisome; next-generation sequencing; bioinformatics Introduction Urothelial carcinoma (UC), arising from the urothelium of the urinary tract, including bladder, ... account the distinct genetic and epigenetic differences in UTUC, recent progress in high-throughput next-generation sequencing (NGS) of the whole genome can achieve good resolution in characterization

Ngày tải lên: 15/01/2020, 16:25

... targets in RA is ongoing [16,18,19] Recent advance in the technique of whole genome sequencing using next-generation sequencing (NGS) method provides unbiased detection of both coding and non-coding ... IGF1 insulin like growth factor PCBP3 poly(rC) binding protein SUSD3 sushi domain containing ARHGDIB Rho GDP dissociation inhibitor beta 0.48 3.21 0.15 ITIH6 inter-alpha-trypsin inhibitor ... Chondrocytes Using Next-Generation Sequencing and Bioinformatics Approaches Yi-Jen Chen1,2, Wei-An Chang1,3, Ling-Yu Wu1, Ya-Ling Hsu4, Chia-Hsin Chen2,5,6,  and Po-Lin Kuo1,7,  1 Graduate Institute

Ngày tải lên: 15/01/2020, 18:29

... Synoviocytes Using Next-Generation Sequencing and Bioinformatics Approaches Yi-Jen Chen1,2, Wei-An Chang1,3, Ling-Yu Wu1, Ching-Fen Huang1,2, Chia-Hsin Chen2,4,5 , Po-Lin Kuo1,6  1 Graduate Institute ... novel therapeutics targeting these small non-coding single-stranded RNAs through intra-articular injection may contribute to the maintenance of joint homeostasis, fine tuning inflammatory and catabolic ... bioinformatics databases, and validated in clinical OA synovium tissue data available in functional genomics data repository We propose the findings will gain novel insights into understanding

Ngày tải lên: 15/01/2020, 19:54

... mapping to individual chromosomes Unique markers were defined as those diagnostic for a single cultivar of the ten included in this study There Table 1 Bread wheat germplasm used for 9 K Infinium ... of genetic diversity in durum wheat Single strand conformation polymorph-ism (SSCP) and KASPar technology were exploited for fine mapping‘Gene of Interest’ in durum wheat lines with bulked segregant ... samples (51 in total) were sub-mitted to the Department of Primary Industries (DPI), Victoria (Australia) for genotyping using the 9 K Infinium SNP array [3,18,22,27] with details presented in Additional

Ngày tải lên: 27/05/2020, 01:22

... gene that encodes two proteins: p16INK4A and p14ARF In physio-logical conditions, p16INK4Ainhibits protein kinase cyclin-dependent kinase 4 (CDK4)/Cyclin D1 (CCND1), which in turn affects the cell-cycle ... progression depending on RB (retinoblastoma susceptibility) protein, while p14ARF inter-feres with the murine-double-minute− 2(MDM2) protein, preventing the degradation of the p53 and favoring its control ... therapy, especially in complex cases with numerous MPMs In this study, a next generation sequencing approach was used to investigate the occurrence of germline and somatic mutations in MPM patients

Ngày tải lên: 17/06/2020, 17:13

... trials for molecular-targeting drugs Recent advances in mutation testing, including next-generation sequencing, have led to the increased use of formalin-fixed paraffin-embedded tissue We evaluated ... Advances in mutation testing for molecular-target-ing drugs, includmolecular-target-ing next-generation sequencmolecular-target-ing (NGS), have led to the increased use of formalin-fixed paraffin-embedded ... provided profiles of cancer-related genes in Japanese patients with esophageal cancer by next-generation sequencing using surgically resected formalin-fixed, paraffin-embedded tissue, and identified

Ngày tải lên: 03/07/2020, 01:43

... AccessDetection of genes mutations in cerebrospinal fluid circulating tumor DNA from neoplastic meningitis patients using next generation sequencing Yue Zhao1†, Jun Ying He1†, Jun Zhao Cui1, Zi-Qi ... the present study aimed to investigate the gene mutations in the CSF ctDNA samples obtained from NM patients using the cutting-edge technique of next generation sequencing (NGS) This approach can ... Cytospin, US) After naturally drying on the slide, the deposit was dyed with May– Grünwald–Giemsa liquid (Yucai, Beijing, China) and Alcian blue staining (Yucai, Beijing, China), according to

Ngày tải lên: 06/08/2020, 05:50

... the adoption of NGS in the clinic We believe acting upon these findings, for instance by organizing educational events, advocating new ways of evidence generation and steering towards the most ... cheap next generation se-quencing (NGS) may confer great benefit in a clinical setting as well, especially in oncology [1] Many insti-tutes are currently developing NGS-based gene panels, which investigate ... Domain Dedication waiver Trang 2In recent years, the cost and time required for large scale sequencing have rapidly decreased, catalyzing an increased understanding of genetic variation in both

Ngày tải lên: 21/09/2020, 11:26

... 2014;17(6):707 –13. 49 DiBardino D, Saqi A, Elvin J, et al Yeld and clinical utility of next-generation sequencing in selected patients with lung adenocarcinoma Clin Lung Cancer 2016;17(6):517 ... al Clinical next-generation sequencing in patients with non-small cell lung cancer Cancer 2015;121(4): 631 –9. 51 Besser J, Carleton HA, Gerner-Smidt P, Lindsey RL, Trees E Next-generation sequencing ... which may result in losing information at the individual level Conclusions The NGS has been finding barriers to its coverage not only in precision therapy, but also in screening for heredi-tary

Ngày tải lên: 28/09/2020, 09:26

... first report of efficient isolation and next-generation sequencing of CTCs in HCC, to our knowledge In this study, ion semicon-ductor next-generation sequencing showed a signifi-cantly higher proportion ... progression Heitzeret al recently reported the first comprehensive genomic profiling of single CTCs using array comparative genomic hybridization and next-generation sequencing in a study of 37 patients ... C L E Open AccessCirculating tumor cells in hepatocellular carcinoma: a pilot study of detection, enumeration, and next-generation sequencing in cases and controls Robin K Kelley1*, Mark Jesus

Ngày tải lên: 30/09/2020, 11:17

... Visualization, Germ-line, Exon-level Background Targeted next-generation sequencing (NGS) is increas-ingly being adopted in clinical laboratories for genomic diagnostic tests [1–6] In addition to single-nucleotide ... Targeted next-generation sequencing (NGS) is increasingly being adopted in clinical laboratories for genomic diagnostic tests Results: We developed a new computational method, DeviCNV, intended ... detecting CNVs in targeted NGS data that includes visualization functionality and con-fidence scores for clinical interpretation Since DeviCNV was developed with the intention of use in clinical

Ngày tải lên: 25/11/2020, 14:46

... can largely be grouped into three main types: sequencing by synthesis, sequencing by ligation and singlemolecule sequencing Sequencing by synthesis involves taking a single strand of the DNA ... WORK 2.1 Next- generation sequencing Next- generation sequencing (NGS) techniques became commercially available around 2005, the first one being the Solexa sequencing technology [27] Since then, ... abiotic stress response and plant pollination Next- generation sequencing has been widely used for understanding plant metabolisms By using next- generation sequencing, draft genomes for unknown species...

Ngày tải lên: 09/09/2015, 11:16

... and is divided into three distinct domains: the N terminal domain (NTD), the deoxyribonucleic acid (DNA) binding domain, and the ligand binding domain (LBD) The “hinge domain” links the LBD to ... CTD-2310E8 RP11-188K5 Invitrogen Invitrogen Invitrogen Invitrogen Invitrogen Invitrogen Invitrogen Invitrogen Invitrogen   33     Methods 6.1 Next generation sequencing (SOLiD4) DNA Extraction ... 2.8 Next Generation Sequencing Next generation sequencing (NGS) has revolutionized science in the past few years It has made a profound impact on the understanding of genetics and biology In research,...

Ngày tải lên: 19/11/2015, 14:04

... tremendous worldwide interest • Integration of this emerging field into engineering and science curriculum is important to prepare the future generation of scientists and engineers ... CMOS inverter out Vdd in p+ Vss Challenges in Nanowire Growth • Uni-directional nanowire growth; vertical or horizontal substrate engineering electric field directed ⇔ ⇔ Understanding of the interfacial ... shock protein (HSP 60) in organisms living at high temperatures (“extremophiles”) is of interest in astrobiology • HSP 60 can be purified from cells as a double-ring structure consisting of 16-18...

Ngày tải lên: 21/02/2014, 17:20

... overall banking system has increased by percentage points since mid 2007 to about 24 per cent, with all of the main groups of banks increasing their use of this funding source During late 2008 ... debt funding and the shift in their funding mix from securitisation to deposits, which is currently a relatively expensive source of funding Banks’ Lending Rates and Margins In setting interest ... banks were facing increasing funding cost pressures For all banks, most of the increase in their lending rates over the cash rate since mid 2007 has been due to their higher funding costs For...

Ngày tải lên: 29/03/2014, 01:20

... between the directions in as-deposited grains and the directions in Rexed grains is therefore im possible in a grain Two of the directions in neighboring grains, which are at right ... IPFs in centeral axis zone of Cu wire drawn by 90% in 14 passes (~15% per pass) through coni cal-dies of in half-die angle, taking strain-hardening per pass into count [31] 17 18 Recent Developments ... preservation and cryo-medicine The authors give us a very clear overview of the state of the art in what is known about inhibiting recrystallization in ice, including a very nice look at inhibition by biological...

Ngày tải lên: 29/03/2014, 21:20

... that the brain may control limb kinematics in a reaching task by shifting the equilibrium points [53] or creating a "virtual trajectory" of the end-point [54], instead of scaling individual muscle ... Virtual training environments can easily display both forms of feedback to inform patients about instant errors in task performance, motivate patients in task learning, and reinforce previous gains ... traumatic brain injury [15,32], cerebral palsy [25,33,34], and incomplete spinal cord injury [16,17] Because this review focuses on new technologies and to avoid repeating past study findings, we...

Ngày tải lên: 19/06/2014, 10:20

... overcome by introducing MIH The MIH provides intelligence to the link layer such as the link going down triggers to wake up FMIP 2.2 Related Work In [10, 11], some schemes of integrating SIP and ... algorithm providing better performance to the multiinterface terminals in the integrated networks can be found in [18] 3.2.4 SIP and MIP Bing Update After sending UNA to the NAR for announcing its existence, ... multimedia subsystem IS: Information server LD: Link down event LGD: link going down event LUP: Link up event MIH: Media independent handover MIIS: Media independent information service NAR:...

Ngày tải lên: 21/06/2014, 11:20