quantitative and qualitative evaluation of protein

... content of total protein (mg g -1 FW) and the % yield of the extraction of soluble proteins (% of extracted soluble proteins respect to the total content) for the roots and the leaves of the plants ... sugars and sucrose in roots (A, C, E and G) and leaves (B, D, F, and H) and chlorophyll content in leaves (I) of Zea mays plants, previously grown in the absence of nitrogen for 17 days (T0) and ... identify 15 and 14 proteins in root and leaf patterns, respectively These proteins and the changes in their accu-mulation are shown in Tables 2 and 3, while further infor-mation of mass spectrometry

Ngày tải lên: 12/08/2014, 03:21

... Including: pages of Pose problems, 33 pages of overview, 30 pages of subjects and research methods, 36 pages of research results, 35 pages of discussion, pages of conclusion and page of petition There ... Glucose – IFG and Impaired Glucose Tolerance – IGT” 1.1.2 Diagnosis and classification of diabetes 1.1.2.1 Diagnosis of diabetes: Standards for diagnosis of diabetes and blood sugar disorder of WHO ... MINISTRY OF EDUCATION AND TRAINING MINISTRY OF PUBLIC HEALTH NATIONAL INSTITUTE OF HYGIENE AND EPIDEMIOLOGY NGUYEN VAN LANH THE SITUATION OF DIABETES, PRE-DIABETES TO THE KHMERIN HAU GIANG PROVINCE AND

Ngày tải lên: 25/07/2014, 11:36

... design of the interview guide, conducted interviews, performed the qualitative analysis, and reviewed a draft of the manuscript RG performed qualitative analysis and reviewed a draft of the manuscript ... MVW and AJC contributed to the design of the study and reviewing and revising the manuscript PJK was the principal investigator of the parent study and contributed significantly to the design of ... really feeling a lot of anxiety and that, and she took my blood pressure, and it was like, 190/113 And that I hadn’t really worried about it until I seen that [letter], and you know, I’m setting

Ngày tải lên: 10/08/2014, 10:22

... 1), and analyzed phenotypes of the 2 day and the 5 day mature DC (Fig 5) The 2 day DC expressed higher levels of class I/II MHC (HLA-I and HLA-DR), cos-timulatory molecules (CD86 and CD40) and ... non-specific response of the ex vivo generated immune cells are necessary Here, using a complete protein-spanning pool of pentadecapeptides of the latent membrane protein 2A (LMP2A) of Epstein-Barr ... analysis soft-ware (GraphPad Softsoft-ware Inc San Diego, CA) and Stu-dent's t-test A 2-sided P value of less than 0.05 was considered statistically significant Results Both IFN-γ and CD137 are

Ngày tải lên: 10/08/2014, 22:20

... design of the interview guide, conducted interviews, performed the qualitative analysis, and reviewed a draft of the manuscript RG performed qualitative analysis and reviewed a draft of the manuscript ... MVW and AJC contributed to the design of the study and reviewing and revising the manuscript PJK was the principal investigator of the parent study and contributed significantly to the design of ... really feeling a lot of anxiety and that, and she took my blood pressure, and it was like, 190/113 And that I hadn’t really worried about it until I seen that [letter], and you know, I’m setting

Ngày tải lên: 11/08/2014, 05:21

... contribution of facilitation to the success of implementation in different types of projects, different types of sites, and with evidence and innovations of varying levels of strength and complexity. ... Veterans Affairs, Office of Research and Development, Health Services Research and Development... collection and analysis, and drafted the initial form and all revisions of the manuscript ... confidentiality and protection of data were adopted. For example, it was agreed that the organizers of the evaluation (CBS, ML, JR-M) would be the collectors and analyzers of raw data and would maintain

Ngày tải lên: 11/08/2014, 05:22

... University of Medicine, Department of Rheumatology and Clinical Immunology were diag-nosed and characterized as recently described [35] and this cohort was used for the evaluation of serology-clini-cal ... 0.0001) At a cut-off of 1.5 RU, the sensitivity and specificity was 36.6%/97.0% for CENP-A and 37.4%/94.8% for CENP-B (Table 2) The highest positive likelihood ratio was 49.1 at a cut-off of 1.75 RU ... [40] and several studies have reported overlap syndromes of SSc and other diseases such as SLE and RA [41] Perhaps of more clinical impor-tance, ACA have been reported to precede the diagnosis of

Ngày tải lên: 12/08/2014, 14:21

... properly cited. mtcPTM - a database of phosphorylated proteins <p>mtcPTM is a new database of phosphorylated protein sequences and atomic models Analysis of the phosphosites in mtcPTM showed ... bondbetween a phosphate group and a hydroxyl moiety of an amino-acid side chain Because of the size and charge of the phosphate groups, their introduction could have a local, and potentially global, ... repository of PTMs in human and mouse proteins that aims to preserve and present the experi-mental evidence that led to the identification of each modifi-cation We show that the graphical display of

Ngày tải lên: 14/08/2014, 07:21

... from that of other eukaryotes. Background Protein domains are the basic building blocks that determine the structure and function of proteins, and they may be con- sidered the units of protein ... ancestors of animals and of deuterostomes (Tables 1 and 2). In animals, protein tyrosine phosphorylation mediated by protein tyrosine kinase plays a crucial role in the processing of signals ... networks and the number of distinct DNA and protein domains per genome Bioinformatics 2001, 17:988-996 Dokholyan N, Shakhnovich B, Shakhnovich E: Expanding protein universe and its

Ngày tải lên: 14/08/2014, 07:21

... expressed protein p is tagged and expressed as a bait and pulls-down proteins p1, ,p k as prey, then the reverse tagging of each protein of p1, ,p k will have a smaller probability of finding ... is the number of proteins, then n + m = , which is the number of all pairs of proteins Any two of these three equations imply the third, and therefore there are three unknowns and two independent ... tested and found. SSA1 PHO3 TEF2 RPC82C Proportions of proteins sampled across datasets Figure 2 Proportions of proteins sampled across datasets This bar chart shows the proportion of proteins

Ngày tải lên: 14/08/2014, 08:20

... spite of extensive investigations on the mode of action of meisoindigo in CML and other malignancies, there are still gaps in our understanding of its mode of action Unlike indirubins where CDK and ... different phases of the cell cycle of K562 cells……… 123 Table 5-5 Effect of 4-5 on the different phases of the cell cycle of K562 cells…….… 125 Table 5-6: Distribution of normal, apoptotic and necrotic ... AND BIOLOGICAL EVALUATION OF DEPARTMENT OF PHARMACY NATIONAL UNIVERSITY OF SINGAPORE Trang 2First and foremost, I would like to dedicate my heartfelt gratitude to my supervisor Associate Professor

Ngày tải lên: 10/09/2015, 08:38

... SYNTHESIS AND BIOLOGICAL EVALUATION OF NATURAL PRODUCTS AND THEIR ANALOGS AS NEW CANCER CHEMOTHERAPEUTIC AGENTS FANG ZHANXIONG NATIONAL UNIVERSITY OF SINGAPORE 2010 SYNTHESIS AND BIOLOGICAL EVALUATION ... friends and family for their unwavering support during these four years i TABLE OF CONTENTS TABLE OF CONTENTS ii SUMMARY iv LIST OF TABLES vi LIST OF FIGURES vii LIST OF SCHEMES ix LIST OF ABBREVIATIONS ... structure of 4-7w and 1D NoE of 4-7p and 4-7r) Figure 4.3 X-ray crystal structure of 4-7w 4.3 Conclusion In summary, we have demonstrated an expeditious and high yielding synthesis of 5-unsubstituted

Ngày tải lên: 10/09/2015, 15:49

... C-termini of mature capsid protein13,27 and NS4A14, and can cleave at internal sites within NS2A and NS3 (Figue 1.2 and Table 1.1) Since the polyprotein processing is a prerequisite for assembly of ... 3TABLE OF CONTENTS SUMMARY v Chapter 2: Biosynthesis of an Acyclic Permutant of Kalata B1 from a Recombinant Fusion Protein with Thioredoxin Trang 42.2.2 Expression and purification of fusion protein ... molecules and receptors and are internalized through endocytosis In the low pH of the endosome, viral glycoproteins mediate fusion of the viral and cellular membranes, allowing disassembly of the

Ngày tải lên: 14/09/2015, 08:44

... UNIVERSITY OF SINGAPORE 2005 PART I TOTAL SYNTHESIS AND BIOLOGICAL EVALUATION OF ANTILLATOXIN AND FRAGMENTS PART II SYNTHETIC STUDIES TOWARDS THE TOTAL SYNTHESIS OF CYTOCHALASANS AND TUBEROSTEMONINE ... PART I TOTAL SYNTHESIS AND BIOLOGICAL EVALUATION OF ANTILLATOXIN AND FRAGMENTS PART II SYNTHETIC STUDIES TOWARDS THE TOTAL SYNTHESIS OF CYTOCHALASANS AND TUBEROSTEMONINE APPENDIX SILICON-ASSISTED ... (B.Sc (Hons), University of Malaya) (M.Sc National University of Malaysia) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARMENT OF CHEMISTRY NATIONAL UNIVERSITY OF SINGAPORE 2005 ACKNOWLEDGEMENTS

Ngày tải lên: 16/09/2015, 08:31

... THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ELECTRICAL AND COMPUTER ENGINEERING NATIONAL UNIVERSITY OF SINGAPORE 2012 Trang 2I hereby declare that this thesis is my original work and it has ... nodes P E i and P E j is denoted by τ ij and ξ ij respectively It is assumed that the time and energy cost of wirelesstransmission is the same at both the sender and the receiver and no techniques ... consumption of P E i in one period of the application π i is given by: Trang 28where T a and T b are connected by the edge E j and x jk and y j are defined as follows:η i = π i The lifetime of the

Ngày tải lên: 30/09/2015, 06:05

... 1NANOPARTICLES OF BIODEGRADABLE POLYMERS FOR CONTROLLED AND TARGETED DELIVERY OF PROTEIN DRUGS SMALL AND MOLECULE DRUGS LEE SIE HUEY NATIONAL UNIVERSITY OF SINGAPORE 2007 Trang 2NANOPARTICLES OF BIODEGRADABLE ... backbone of PLA and PLGA to form PLA-TPGS and PLGA-TPGS block copolymers These block copolymers are an example of amphiphiles Their uses as different carriers for delivery of protein and anticancer ... (Mu and Feng, 2002; 2003; Feng et al., 2004; Khin and Feng, 2005; Khin and Feng, 2006) Recently, many studies on the use of blends and copolymerization of PLA or PLGA with PEG for peptide/protein

Ngày tải lên: 26/11/2015, 22:41

... Trang 5of the benzamidine and thiocarbazone moieties in the proligand Additionally, a high field shift of the signal of the NH proton of the CS-NHMe residue from 7.64 ppm in the proligand [13] ... 1H NMR spectrum of 4 shows the same features as those of the nickel, palladium and platinum compounds: the absence of two N-H resonances and a high field shift of the N-H signal of the CS-NHMe ... spectrum of 4 is similar to those discussed for the complexes 1 - 3 with a sharp medium absorption band of the N-H stretch at 3387 cm-1 and a strong bathochromic shift of the C=N band with

Ngày tải lên: 12/12/2017, 06:47

... we decided to estimate the potential of another promising candidate of a pentadentate ligand (H3L) first by DFT calculations [18] H3L* and H3L are related ligand systems They provide the same donor ... experimental and optimized bonding parameters in [ReO(L*Et) underlines the reliability of performed DFT calculations and Table 1. Energies of optimized geometries of [ReO(LEt)] and [ReO(L*Et)] ... inequality of the two residues R, and consequently the 1H NMR spectrum of H3LMorph shows two overlapping signals with complex coupling patterns of two NCH2 groups and two OCH2 groups of the morpholine

Ngày tải lên: 16/12/2017, 09:19

... treatment of the psychotic symptoms of schizophrenia ~-3 Antagonism of DA receptors is a major component of the mechanism of action of classical antipsychotic agents such as haloperidol and chlorpromazine ... functionality of the previous series, and a variety of 5-, 6-, and 7-aminoalkoxy-benzopyran-4-ones were synthesized and evaluated for their activity at the DA D2 receptor 66A sample of these compounds ... functionality of compounds such as 68 was to use a heterocycle such as a pyrimidine in place of the amide carbonyl Preparation of compounds of this type led to the discovery of an interesting series of

Ngày tải lên: 29/12/2017, 17:14

... atoms: C t of the C-terminus, C c of Asp and C d of Glu, and O g of Tyr. Positive unit charges were added at the following atoms: N t of the N-terminus, N f of Lys, C f of Arg, and N d or N e of His ... A ˚ . The parameter set of charges and van der Waals radii PARSE [24], dielectric constants of 78.4 and 20.0, for solvent and protein, respectively, temperature of 298 K, and ionic strength corresponding ... stability and packing of the dimer. Parallel packing of the aromatic side chains Tyr16, Phe31, Tyr35 and Phe36 and contacts involving the side chains of Leu12, Val20, Leu28, Val29, Val33, Thr37 and...

Ngày tải lên: 08/03/2014, 22:20