pylori binding to immobilized lewis b glycoconjugate

... 2007 FEBS 4137 All lens b- crystallins are calcium -binding < /b> proteins M K Jobby and Y Sharma A A B B Fig Stains-All binding < /b> to < /b> (A) bB2- and (B) bA3-crystallins: 100 lg of either bB2- or bA3-crystallin ... calorimetry Probing calcium -binding < /b> by Stains-All assay Calcium -binding < /b> to < /b> bB2- and bA3-crystallins was evaluated by calcium probe Stains-All, a carbocyanine dye [29] The dye binds the recombinant bA3- ... provide a B Fig Isothermal titration calorimetry: (A) calcium -binding < /b> isotherm of bB2-crystallin (B) Terbium binding < /b> isotherm of bA3-crystallin The best fit to < /b> four-site sequential binding < /b> model...

Ngày tải lên: 18/02/2014, 16:20

... anesthetics can bind to < /b> a-helical proteins [5–12,27,28], but this is the first study to < /b> demonstrate binding < /b> to < /b> a b- barrel protein using direct binding < /b> assays Isoflurane has been shown to < /b> bind to < /b> bovine ... single-site binding < /b> model 575 General anesthetic binding < /b> to < /b> a b- barrel protein J S Johansson et al Fig Competition between halothane and 1-aminoanthracene (AMA) for binding < /b> to < /b> porcine odorant binding < /b> ... energy of binding < /b> (DG°) exceeded the heat of binding < /b> (DH°) by more than a factor of two (Table 1), indicating that binding < /b> to < /b> porcine odorant binding < /b> protein is entropy driven, in contrast to < /b> the...

Ngày tải lên: 07/03/2014, 16:20

... B subunit families The predicted A subunit binding < /b> domains in B/ B55 and B ¢/PR72 were also able to < /b> interact with the PP2A A subunit The presence of a conserved motif in the highly divergent B ... lysate-synthesized B fragments [10,21] To < /b> optimize conditions for the assay, fulllength B5 6a was ®rst tested for binding < /b> to < /b> GST-A B5 6a full-length protein bound well to < /b> GST-A, but not to < /b> GST alone (Fig 1A) To < /b> ... lysate may contribute to < /b> the formation of heterotrimers [27] To < /b> map the region(s) of B5 6a responsible for binding < /b> to < /b> the A subunit, multiple B5 6 fragments were generated by PCR followed by in vitro...

Ngày tải lên: 08/03/2014, 10:20

... Mammal Bacterium Mold Yeast Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Yeast Bacterium ... Archaea Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Plant Plant a-Amylase Barley (AMY1) Barley (AMY2) Rice (2A) Maize Wheat (AMY3) Black ... complexes in which the entire binding < /b> site, as dened by subsite mapping, was occupied by an inhibitory substrate analog [22,23] Even though the validity of subsite mapping is debated, these crystal structures...

Ngày tải lên: 31/03/2014, 08:20

... prescribed for those found to < /b> be infected with H pylori < /b> by a 13C-urea breath test or serum anti-H pylori < /b> antibody More than one month after the medication, a 13C-urea breath test was conducted to < /b> ... different proton pump inhibitors, differences in CYP2C19 genotype and antibiotic resistance on the eradication rate of Helicobacter pylori < /b> infection by a 1-week regimen of proton pump inhibitor, amoxicillin ... (dichotomous) and age (a continuous variable) using an unconditional logistic regression model Results Table describes the backgrounds of the whole visitors and participants From July 2004 to < /b> October...

Ngày tải lên: 31/10/2012, 16:57

... are shown in Table 1, where it can be seen that binding < /b> is inhibited most effectively by unlabeled PCSK9-D374Y Unlabeled PCSK9-D374Y was also able to < /b> reduce the binding < /b> of its labeled counterpart ... specific binding < /b> difficult To < /b> overcome the problem of low specific binding,< /b> we used labeling by [125I]TC, which reacts with lysines, and we found [125I]TC labeling of PCSK9 to < /b> be more suitable for ... of bound antibody against LDLR was measured by subsequent binding < /b> of 125I-labeled goat anti-(rabbit IgG) (10 lgÆmL)1) A control in which the anti- Characterization of the binding < /b> of PCSK9 to...

Ngày tải lên: 14/02/2014, 14:20

... substrate binding < /b> by hydrogen bond formation, and its dipole facilitates substrate binding < /b> as well The hydrogen bond between the backbone nitrogen atom of Asp291 and the 3phosphate of the substrate ... freedom for the bulky phytate to < /b> rotate for further dephosphorylation steps or for alternative substrates to < /b> bind to < /b> the active site Indeed, PhyK is able to < /b> dephosphorylate a number of substrates including ... substrate binding < /b> motif, 290-HD-291, are conserved and in close proximity In order to < /b> orient His25 for the nucleophilic attack on the substrate, the Nd atom donates a hydrogen bond to < /b> the backbone...

Ngày tải lên: 16/02/2014, 09:20

... dCTP deaminase was also investigated by equilibrium binding < /b> This revealed a hyperbolic binding < /b> curve (Fig 4D) with a stochiometry of : of dTTP bound per subunit of dCTP deaminase 4192 Mutational ... deaminase is activated by dCTP and inhibited by dTTP and both nucleotides act on the enzyme by binding < /b> to < /b> an allosteric site to < /b> alter the cooperativity of dCMP binding < /b> [5,29,30] The activity of dCTP ... corresponding to < /b> the presence of a given concentration of inhibitor I and I0.5 is the concentration of inhibitor for half-maximal inhibition Equation (3) was used for hyperbolic binding < /b> of ligands to < /b> the...

Ngày tải lên: 18/02/2014, 16:20

... delta-like ⁄ 4; EFNB1 ⁄ 2, ephrin -B1 ⁄ 2; ERBB4, receptor protein tyrosine kinase erbB4; GHR, growth hormone receptor; IGF1R, insulin-like growth factor receptor; IL1R2, interleukin receptor II; JAG1, ... The strongly hydrophobic segment (VTAFYWAL) that is expected to < /b> be embedded in the membrane and to < /b> become exposed to < /b> the solvent upon cleavage of Jagged-1 is not sufficient to < /b> promote folding of ... from 355 to < /b> 345 nm (Fig 5) Altogether, fluorescence data confirm binding < /b> of J1_tmic to < /b> SDS micelles and DMPG phospholipid vesicles, with at least partial embedding of one or both the tryptophan residues...

Ngày tải lên: 18/02/2014, 16:20

... NPbfuc NPbglc NPbgal NPbxyl MUbglc NPbfuc NPbglc NPbgal NPbxyl MUbglc NPbfuc NPbglc NPbgal NPbxyl MUbglc NPbfuc NPbglc NPbgal NPbxyl MUbglc NPbfuc NPbglc NPbgal NPbxyl MUbglc NPbfuc NPbglc NPbgal ... software MUbglc, methylumbeliferyl b- glucoside; NPbfuc, p-nitrophenyl b- fucoside; NPbgal, p-nitrophenyl b- galactoside; NPbglc, p-nitrophenyl b- glucoside; NPbxyl, p-nitrophenyl b- xyloside Enzyme Substrate ... reductions (about 40%) of that unfavorable interaction, but they did not convert it to < /b> a favorable binding < /b> Thus, by contrast with the binding < /b> of alkyltype aglycones, single mutations were unable to < /b> cause...

Ngày tải lên: 18/02/2014, 17:20

... explained as being due to < /b> exciton splitting between the symmetric pairs of [Cd(Scys)4]2 groups in the Cd4(Scys)11 binding < /b> site [27] As noncooperative metal binding < /b> is predicted to < /b> result in the ... metal binding < /b> dictates that the Keq must be decreasing as the sequential reaction proceeds Finally, K3a and K4a for the a domain would have to < /b> be greater than K 2b and K 3b for the b domain, to < /b> explain ... incoming metal ion would be to < /b> form a bridging interaction in the a domain or to < /b> form another independent tetrathiolate site in the b domain It is probable that the equilibrium constant for the...

Ngày tải lên: 19/02/2014, 00:20

... observed binding < /b> enthalpy, DHbinding is the buffer-independent binding < /b> enthalpy, DHionization is the ionization enthalpy of the buffer, and n is the net number of protons transferred during binding < /b> ... have been shown not to < /b> be essential for binding < /b> [20] At the time we initiated our calorimetric and biophysical studies, we synthesized both human and rabbit peptides, and observed that the rabbit ... in binding < /b> may be due to < /b> binding < /b> of N-loop residues to < /b> the receptor N-domain The structure of IL-8 is known, and the structural basis for its function has been well studied [8–17] The receptor...

Ngày tải lên: 19/02/2014, 05:20

... the ability of stefin B to < /b> bind copper was assessed We used isothermal titration Copper binds to < /b> cystatin B calorimetry to < /b> monitor the binding < /b> event at pH and pH It was found that the protein binds ... cases best fit parameters were obtained from the sequential binding < /b> sites model, whereby the user defines the number of binding < /b> sites to < /b> be fitted in a sequential manner Attempts to < /b> fit data to < /b> anything ... 2006 FEBS ˇ E Zerovnik et al by these ions [41] which would not be beneficial in the light of higher toxicity of such aggregates [3] The discovery that copper binding < /b> to < /b> stefin B inhibits fibril formation...

Ngày tải lên: 19/02/2014, 06:20

... contribute to < /b> high-affinity binding < /b> of the ephrin B cytoplasmic domain to < /b> the Grb4 adaptor protein [11] The equivalent Tyr304 residue of Xenopus ephrin B1 was shown to < /b> be essential for binding < /b> to < /b> ... specific binding < /b> of ephrinB2(301–333) to < /b> the Grb4 SH2 domain The fluorescence binding < /b> experiments also indicate that the Cys301 residue may not be required for binding < /b> as it is labeled by the bulky ... the phosphotyrosine binding < /b> pocket of the Grb4 SH2 domain is almost identical to < /b> that of the Src SH2 domain, being composed of residues in the bA2, bB5, bD3, bD4, bD6 and bD¢1 structural elements...

Ngày tải lên: 19/02/2014, 12:20

... determined spectrophotometrically using their molar absorptivities at the kmax of the Soret band, as described [17] Porphyrin binding < /b> Porphyrin binding < /b> to < /b> MCL was investigated by the absorption titration ... hydrophobic, such as adenine, 2,6-toludinylnaphthalenesulfonic acid, auxins and cytokinins, to < /b> a variety of plant lectins [40–44] and the binding < /b> of H2TPPS to < /b> human serum albumin and b- lactoglobulin ... porphyrin (CuTMPyP) have also been delineated by variable temperature studies [18] It has been found that the binding < /b> of these two porphyrins to < /b> TCSL is largely driven by favorable entropic forces and...

Ngày tải lên: 19/02/2014, 16:20

... has an inhibitory effect on binding < /b> of poly(A) by PNPase In contrast, an interaction between Hfq and PNPase would be expected to < /b> inhibit both initiation and elongation of the tail FEBS Journal ... activity of FEBS Journal 272 (2005) 454–463 ª 2004 FEBS M Folichon et al A Hfq binding < /b> to < /b> RNA stimulates elongation by PAP I B Fig A monophosphate 5¢ end stimulates polyadenylation but not Hfq binding < /b> ... extends to < /b> mRNA the previous data obtained with small regulatory RNA (Fig 5B) Hfq inhibits poly(A) synthesis by polynucleotide phosphorylase Because Hfq binds to < /b> the 3¢ end of RNA harboring a...

Ngày tải lên: 19/02/2014, 16:20

... warfarin binding < /b> Reciprocally, heme binding < /b> to < /b> HSA decreases warfarin affinity by the same extent [19] Fe(III)heme allosterically inhibits ligand binding < /b> to < /b> Sudlow’s site I, possibly by stabilizing ... isosbestic points indicate that the binding < /b> equilibrium occurs through only two forms, the HSA-free and the HSA-bound Mn(III)heme (Fig 3B) 4676 Fig (A) Binding < /b> isotherms for Mn(III)heme binding < /b> to < /b> ... competitively binds to < /b> FA1, but also binds to < /b> a different site(s) and induces conformational changes that lowers the equilibrium constant for Mn(III)heme binding < /b> to < /b> the FA1 site by a factor of 10–500...

Ngày tải lên: 20/02/2014, 02:22

... CD1 1b I domain inhibits red cell binding < /b> to < /b> purified integrin Red cells bind poorly to < /b> CD11a/CD18 but more efficiently to < /b> CD1 1b/ CD18 [28] Therefore, we tested inhibition of erythrocyte binding < /b> to < /b> ... of mAb 60.1, which binds to < /b> the recombinant CD1 1b I domain These data indicate that there might be an unidentified ligand in red cells that could mediate binding < /b> to < /b> CD1 1b and probably also to < /b> CD11a ... erythrocytes to < /b> CD11a I domain was effectively blocked by monoclonal antibodies to < /b> ICAM-4 and the CD11a I domain (Fig 1B) The mAb 7E3 and the mAbs BS46 and BS56 to < /b> ICAM-4 partially but significantly inhibited...

Ngày tải lên: 20/02/2014, 11:20