proteomic analysis of human mesenchymal stem cells

... human stem cells cannot automatically be considered to behave in a similar way to other adherent cells lines This is because with human stem cells it is the cell itself that forms the basis of ... 10.6 cells/bead; b day 2, 10.7 cells/bead; c day 3, 6.2 cells/bead; d day 4, 9.5 cells/bead; e day 5, 19.7 cells/bead; f day 7, 52.1 cells/bead; g day 8, 67.6 cells/bead; h day 9, 98.4 cells/bead; ... of human stem cells is not a trivial exercise Indeed, expanding human stem cells in culture is currently more of an art than a science so much so that some laboratories cannot or do not grow human

Ngày tải lên: 02/11/2022, 10:41

... Trang 1Integrated analysis of DNA methylome and transcriptome reveals the differences in biological characteristics of porcine mesenchymal stem cells Zheng Feng1, Yalan Yang1, ... main sources of mesenchymal stem cells (MSCs) These two MSCs display significant differences in many biological characteristics, yet the underlying regulation mechanisms of these cells remain ... and gene expression profiles of porcine MSCs were generated The osteogenic and adipogenic differentiation ability of porcine BMMSCs is greater than that of UCMSCs A total of 1979 genes were differentially

Ngày tải lên: 30/01/2023, 20:29

... specific diseases in the course of ex vivo gene therapy 1.1.1 Human Embryonic Stem Cells Human embryonic stem (hES) cells are cells derived from the inner cell mass of human blastocysts The hES cell ... GENETIC MODIFICATION OF HUMAN EMBRYONIC STEM CELLS Du Juan NATIONAL UNIVERSITY OF SINGAPORE 2008 Trang 2BACULOVIRUS-MEDIATED GENETIC MODIFICATION OF HUMAN EMBRYONIC STEM CELLS DU JUAN (B Sc.) ... TRANSDUCTION OF HUMAN EMBRYONIC STEM CELLS Trang 282.1 Introduction hES cells hold great potential as a system for the study of lineage specification and as an unlimited source of cells for the

Ngày tải lên: 11/09/2015, 14:38

... safe xenofree cryopreservation method for the storage of human embryonic stem cells Stem Cells 22: 779-89 Richards M, Tan SP, Tan JH, Chan WK, Bongso A (2004) The transcriptome profile of human ... undifferentiated growth of human embryonic stem cells Stem Cells 21:546-556 Richards M, Fong CY, Chan WK, Wong PC, Bongso A (2002) Human feeders support prolonged undifferentiated growth of human inner cell ... Characterization of Neural Stem Cells from the Adult Human Olfactory Bulb Stem Cells 18: 295-300 Palmer TD, Schwartz PH, Taupin P, Kaspar B, Stein SA, Gage FH (2001) Progenitor cells from human brain

Ngày tải lên: 16/09/2015, 15:55

... the bone marrow (mesenchymal stem cells, hematopoietic stem cells) and (2) embryonic stem and embryonic germ cells derived from discarded human embryos and germ line stem cells. Adult stem cells can ... marrow (mesenchymal stem cells, hematopoietic stem cells) and (2) embryonic stem and embryonic germ cells derived from discarded human embryos and germ line stem cells Adult stem ... specific differentiation of hES cells is mandatory. 1.5 Human Embryonic stem cells As the name suggests, the human embryonic stem cells are derived from the early embryo. These cells have the capacity

Ngày tải lên: 09/10/2015, 11:24

... Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of embryos of blastocyst ... Culture of human ES cell line 2.1.1 Preparation of feeder cells 2.1.2 Maintenance of human ES cells 2.1.3 Preparation of embryoid bodies 2.2 Culture of NCCIT cell line 2.3 Preparation of shRNA ... completion of my research Trang 31.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory networks and transcription factors in human ES cells

Ngày tải lên: 16/10/2015, 11:58

... detection of activity of alkaline phosphatase using a Lumi-Phos kit (Pierce Biotechnology) The visu-alized bands of western blot were quantified with Bio-Rad QUANTITY ONE software The volumes of target ... process of cancer cells and are potentially useful for cancer therapy due to easy accessibility of the targets Methods: In the present research, TMT method followed by mass spectrometry analysis ... contributors 1 Key Laboratory of Living Donor Liver Transplantation, Ministry of Public Health, Department of Liver Transplantation Center, The First Affiliated Hospital of Nanjing Medical University,

Ngày tải lên: 29/09/2020, 16:18

... and toxicity of O6 -methylguanine, and of MGMT, responsible for the removal of the O6-methyl adduct In fact, the lack of ex-pression of one of MMR components and/or the presence of high MGMT ... densities (O D.) of the protein of interest and those of tubulin The results are representative of one out of two experiments with similar results SJ: SJGBM-2 Panel C Analysis of MGMT activity ... activity was evaluated as fmol of32P-NAD+/μg of protein MGMT activity assay and bisulfite sequencing analysis of MGMT promoter methylation Cells (1×106) were lysed in 0.5 ml of a buffer containing 0.5%

Ngày tải lên: 05/11/2020, 01:28

... haematopoietic stem cells Nodal Member of the TGF-beta superfamily Anterior-posterior and visceral endodermal patterning Gfap Component of intermediate filaments of glial cells of the astrocyte ... sample of cells A frequently discussed matter in ultrasound trapping is the viability of trapped cells after being exposed to ultrasound.Nyborg (2001)reviewed the 80-year history of studies of biologic ... acquisition and processing software (Soft Imaging System, SIS, GmbH) Experimental procedure Single cell suspensions of ES cells were prepared as described above and diluted to 3000 cells/mL The ultra-sound

Ngày tải lên: 02/11/2022, 10:47

... differentiation of human adipose stem cells into functional hepatocytes Jieqiong Huang*, Xinyue Guo*, Weihong Li & Haiyan Zhang The generation of hepatocytes that are derived from human adipose stem cells ... presented in the cells of four groups The percentage of cells with LDL partiparti-cles in cells of Chir99021 at day 20 was relatively higher than other groups, which resembles the human hepatocytes ... expression of FOXA2 and CXCR4 in cells was examined by flow cytomet-ric analysis The data showed that the percentage of FOXA2 positive cells in four treated group was 78.8% (activin A-treated cells),

Ngày tải lên: 19/11/2022, 11:45

... differentiation of hMSCs and degradation of the hydrogels [21,34,35] Human mesenchymal stem cells (hMSCs) have been shown to benefit from the presence of fibronectin in the early stages of chondrogenic ... effect of RGDS peptide release In effect, the presence of the MMP7–cleavable moieties in the hydrogels and the action of encapsulated cells via the release of MMP7 enables the release of RGDS ... ng/mL recombinant human MMP7) Release of the RGDS peptide from the hydrogels was evaluated over time in the absence of cells using cystamine–FITC as a model substrate in place of the RGDS peptide,

Ngày tải lên: 24/11/2022, 17:41

... differentiation of adult stem cells. Regenerative medicine is a multidisciplinary field aiming to regenerate tissues by combining biological factors and engineering fundamentals1 Recently the use of stem cells ... lineages2,3 Human mesenchymal stem/stromal cells (hMSCs) can undergo chondrogenic, osteogenic and adipogenic differentiation3, among others, and are not asso-ciated to the ethical concerns of other stem ... differentiation of hMSCs in certain areas of the construct, gradients of growth factors27 and material stiffness28 were generated To the best of our knowledge, no studies have linked the stem cell

Ngày tải lên: 04/12/2022, 10:32

... number of CDCs for experiments, regardless of age To characterize the phenotype of CDCs, the percentage of cells positive for CD90 and CD105, which are validated markers of mesenchymal stem cells14, ... the function of cardiac stem cells In addition, these factors do not necessarily influ-ence the quantity and quality of stem cells uniformly For example, although hematopoietic stem cells from ... determinant of the quantity and quality of CDCs Senescent cells accumulate in tissues with advancing age Tissue-specific stem cells, for example those of the hematopoietic and musculoskeletal system

Ngày tải lên: 04/12/2022, 14:53

... MSCs A num-ber of previous studies involving intra-articular injection of exogenous cells have observed varying levels of car-tilage repair coupled with low numbers of engrafted cells at the injury ... exist Mesenchymal stem cells (MSCs) have been isolated from a variety of tissues including bone marrow (BM)1, adipose tissue2, synovium and synovial fluid3 and periosteum4 Indeed, the use of BM-MSCs ... & Joint Health, University of Calgary, Calgary, AB, Canada 2University of Calgary, Department of Surgery, Calgary, AB, Canada 3University of Calgary, Department of Radiology, Calgary, AB, Canada

Ngày tải lên: 04/12/2022, 14:57

... differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem ... with human mesenchymal stem cells Tissue Eng 2002, 8:131-144 24 Johnstone B, Hering TM, Caplan AI, Goldberg VM, Yoo JU: In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells ... Research (IZKF) Würzburg References Kolf CM, Cho E, Tuan RS: Mesenchymal stromal cells Biology of adult mesenchymal stem cells: regulation of niche, selfrenewal and differentiation Arthritis Res Ther...

Ngày tải lên: 09/08/2014, 14:22

... differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved stem cells ... differentiation of human mesenchymal stem cells into insulin producing cells J Biomed Mater Res A 2008, 86(4):1097-1105 Chao KC, Chao KF, Fu YS, Liu SH: Islet-like clusters derived from mesenchymal stem cells ... 53(7):1721-1732 Page 10 of 10 doi:10.1186/1423-0127-17-56 Cite this article as: Lin et al.: Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through...

Ngày tải lên: 10/08/2014, 05:21

... NANOFIBERS FOR OSTEOGENIC DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS NGUYEN THI HIEN LUONG (B.Eng., Ho Chi Minh city University of Technology, Vietnam) A THESIS SUBMITTED FOR THE DEGREE OF ... 43 2.3 Stem cells 51 2.3.1 Definition and classification 51 2.3.2 Stem cells in tissue engineering 53 2.3.3 Mesenchymal stem cells 54 v 2.4 Stem cell ... there are issues raised on accessibility of tissue sites where the mature cells can be harvested [23-30] Among several types of stem cells, mesenchymal stem cells (MSCs) have been shown to be an...

Ngày tải lên: 09/09/2015, 10:07

... culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube baby ... summarizing 12 years of our team’s experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The ... translational research in the field of embryonic and induced pluripotent stem cells It commences with practical aspects of the derivation and growth of human embryonic stem cells from inner cell mass...

Ngày tải lên: 22/03/2014, 09:20

... 13 The expression of miR-92 has been reported in human embryonic stem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine ... The tested adult cells were Human Microvascular Endothelial Cells (HMVEC), Human Umbilical Vein Endothelial Cells (HUVEC), Umbilical Artery Smooth Muscle Cells (UASMC), Normal human astrocytes ... undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational Medicine 2009, 7:20...

Ngày tải lên: 18/06/2014, 15:20

... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according ... isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in ... vitro culture is capable of sustaining a large number of cells, which can confer specific advantages and disadvantages The use of freshly isolated human hematopoietic stem cells from thalassemic...

Ngày tải lên: 18/06/2014, 19:20