protein dscr 1 deters cancer and septic inflammation
microrna 124 mir 124 inhibits cell proliferation metastasis and invasion in colorectal cancer by downregulating rho associated protein kinase 1 rock1
... MiR-124 and ROCK1 in Colorectal cancer cell lines We next determined the expression levels of miR-124 and ROCK1 (mRNA and protein) in Three Colorectal cancer cell lines (HCT116, HT29 and SW620) and ... interest exist Trang 102011;61:69-90. 2009;13:1379-1388. 2011;38:734-742. bedside Carcinogenesis 2011;32:247-253. regulation Oncogene 2006;25:6163-6169. 2006;6:259-269. 10 Lu Z, Liu M, Stribinskis ... 2014;14:1103-1111. 22 Zhang W, Zou C, Pan L, Xu Y, Qi W, Ma G, Hou Y, Jiang P: MicroRNA-140-5p inhibits the progression of colorectal cancer by targeting VEGFA Cell Physiol Biochem 2015;37:1123-1133.
Ngày tải lên: 02/11/2022, 14:29
Báo cáo y học: " Characterization of protein tyrosine phosphatase H1 knockout mice in animal models of local and systemic inflammation" docx
... (#Mm00441242_m1, Applied Biosystems), IL1b (#Mm01336189_m1, Applied Biosystems), IL12a (#Mm00434169_m1, Applied Biosystems), IL6 (#Mm00446190_m1, Applied Biosystems), TNF (#Mm00443258_m1, Applied ... MCP-1 and IL10 be-tween WT and KO LPS-treated animals Both WT and KO mice displayed a LPS-induced increase in TNFα, MCP-1 and IL10; IL6 level was significantly increased by LPS in both WT and ... plasma 180 minutes after 1 mg/kg LPS injection a) PTPH1- WT and KO mice displayed a LPS-induced increase in TNFα and IL10; b) MCP-1 and IL6 levels were significantly increased by LPS in both WT and
Ngày tải lên: 11/08/2014, 03:20
Báo cáo y học: " Early lactate clearance is associated with biomarkers of inflammation, coagulation, apoptosis, organ dysfunction and mortality in severe sepsis and septic shock" docx
... 9.1 0.13 Hemoglobin (g/dL) 11.6 ± 2.6 11.0 ± 2.9 11.5 ± 2.5 11.3 ± 2.7 0.68 Platelets (×103per μL) 163.7 ± 82.2 184.0 ± 116.7 254.1 ± 135.6 244.4 ± 124.8 <0.01 Glucose (mg/dL) 303.5 ± 421.4 ... 0.5 1.2 ± 0.6 0.76 Respiratory rate (breaths per min) 29.5 ± 10.3 30.4 ± 10.9 34.2 ± 11.8 32.2 ± 10.9 0.16 Laboratories White blood cells (×103per mm3) 11.8 ± 7.1 13.5 ± 8.9 15.0 ± 10.5 15.6 ... 243.4 85.4 ± 121.9 <0.01 TNF- a (pg/mL) 65.2 ± 105.9 50.9 ± 69.2 47.4 ± 72.8 19.6 ± 19.8 <0.01 ICAM-1 (ng/mL) 409.1 ± 208.1 413.3 ± 204.5 379.7 ± 213.8 299.2 ± 156.1 <0.01 Caspase-3 (ng/mL)
Ngày tải lên: 11/08/2014, 08:22
Báo cáo y học: " Rice black-streaked dwarf virus P6 self-interacts to form punctate, viroplasm-like structures in the cytoplasm and recruits viroplasm-associated protein P9-1" docx
... for P6-P9-1 interaction A dozen P9-1 BD-fused deletions that express fusions BD-P9-11-197, BD-P9-11-207, BD-P9-1 1-248 , BD-P9-176-347, BD-P9-1167-347, BD-P9-1198-347, results indicated that all ... the major capsid and outer capsid, respectively [8,14,15] Both S7 and S9 encode nonstructural proteins S7 ORF1 P7-1 and S9 ORF1 P9-1 are components of the tubular structures and viroplasm produced ... possesses A SD/AHWL AD-P6 1-449 341-792 271-703 B 274-703 395-703 395-659 1-197 1-207 1-248 76-347 BD-P9-1 167-347 198-347 208-347 76-207 Figure 7 Investigation of P6-P9-1 interaction in YTH
Ngày tải lên: 11/08/2014, 21:21
Báo cáo y học: "Dephosphorylation of CDK9 by protein phosphatase 2A and protein phosphatase-1 in Tat-activated HIV-1 transcription" doc
... CDK9/cyclin T1, an RNA polymerase II C-terminal domain kinase [6,11,12] and histone acetyl transferases [13-15]. Whereas P-TEFb induces HIV-1 tran- scription from non-integrated HIV-1 template [6,11,12], ... -+ OA, nM - - 0.1 0.3 1 3 10 30 100 30% 50 60 70 80 90 100 IC 50 = 4 nM Transactivation, %of control Okadaic acid, nM 0.1 1 10 100 1000 B Okadaic Acid, nM 1 2 3 4 5 6 7 8 9 C 1 2 3 4 5 6 7 8 ... NIPP1. A DNA, ng Transactivation, Fold 1, no Tat 2, Tat 3, Tat+NIPP1 4, Tat+NIPP1 mut 0 10 20 30 40 50 60 0 50 100 B DNA, ng Transactivation, Fold 0 0.4 0.8 1.2 1.6 0 50 100 JK2+NIPP1 JK2+ NIPP1mut
Ngày tải lên: 13/08/2014, 09:21
Báo cáo y học: "Recombinant human activated protein C attenuates cardiovascular and microcirculatory dysfunction in acute lung injury and septic shock" ppt
... 60 80 100 120 160 180 200 220 240 # # * * 140 // 60 80 100 120 140 160 180 200 # # * * Time (h) // p ≤ 0.05; # vs BL, *vs Control, †vs Sham SHAM CONTROL RhAPC 100 Figure ... # * * † * 80 90 100 110 120 130 140 150 # # # * † † Time (h) SHAM CONTROL RhAPC 4 5 6 7 8 9 # † # # * † † 3 Time (h) 40 60 80 100 120 140 # * // Time (h) ... recombinant human activated protein C P ≤0.05: # versus baseline,†versus sham, *versus control. 80 100 120 140 160 Time (h) * // 0 200 400 600 800 1000 1200 # * † * † * †
Ngày tải lên: 14/08/2014, 07:21
Báo cáo sinh học: "A dual function fusion protein of Herpes simplex virus type 1 thymidine kinase and firefly luciferase for noninvasive in vivo imaging of gene therapy in malignant glioma" ppsx
... methods: (1) the technique is very sensitive (possibly 10-15 – 10-17 mole of luciferase/L are detectable in vivo, [13]) and detects tumor cells at a stage where radiography and PET cannot [14,15], ... reso-nance imaging, and optical imaging methods using fluo-rescence and bioluminescence were introduced and increasingly used for temporal and spatial monitoring of transgene expression [11-13] Bioluminescence ... Genetic Vaccines and Therapy 2004, 2:7 doi:10.1186/1479-0556-2-7 Received: 05 April 2004 Accepted: 04 August 2004 This article is available from: http://www.gvt-journal.com/content/2/1/7 © 2004 Söling
Ngày tải lên: 14/08/2014, 19:22
Proline rich acidic protein 1 in life and death 2
... 138 3.11.1.2 Effects PRAP1 on actin filament 139 3.11.1.3 Effects of PRAP1 on microtubules 141 3.11.2 PRAP1 interacts with Hsp 70 141 3.12 Role of PRAP1 in apoptotic cells 145 3.12.1 Induction ... 5-FU 136 Trang 103.11 Other mechanisms employed by PRAP1 inhibition is enhancing cell death 138 3.11.1 Effect of PRAP1 on cytoskeleton 138 3.11.1.1 Effects of PRAP1 on cellular morphology 138 ... SLE 156 3.13.1 Detection of PRAP1 autoantigen 156 3.13.2 Detection of PRAP1 autoanitbodies 158 3.13.3 PRAP1 and proinflammatory cytokines 158 3.13.4 PRAP1 expression in PBMC 161 3.14 Regulation
Ngày tải lên: 11/09/2015, 16:05
Proline rich acidic protein 1 in life and death 3
... Trang 1CHAPTER THREE RESULTS Trang 23.1 PRAP1 and intestinal differentiation 3.1.1 PRAP1 is expressed in epithelial cells of the intestines The expression of PRAP1 in human intestinal ... of PRAP1 is at the transcriptional level, various lengths of PRAP1 sequence upstream of its transcription start site were amplified from Bac clone (RP11-122K13) as shown in Figure 3.19 Each ... (N-terminal truncated TCF) for 24, 48 and 72 hr Figure 3.14 PRAP1 is induced in differentiated colorectal cancer cells Representative western blot of PRAP1 and GAPDH (loading control) in L8 cells
Ngày tải lên: 11/09/2015, 16:05
Proline rich acidic protein 1 in life and death 4
... Suppression of PRAP1 induction by 5-FU at protein level Representative western blot of PRAP1 and GAPDH in HCT 116 after transfection with PRAP1 specific siRNA (PRAP1 siRNA1 and 2) or PRAP1 siRNA specific ... prap1 and gapdh in HCT 116 after transfection with PRAP1 specific siRNA (PRAP1 siRNA1 and 2) or PRAP1 siRNA specific control siRNA (control siRNA1 and 2) for 48 hours, and followed by the addition ... with 5-FU and CPT at the indicated doses for 24, 48 and 72 hours Figure 3.38 Early upregulation of PRAP1 protein by 5-FU and CPT Representative western blot of PRAP1 and GAPDH in HCT116 cells
Ngày tải lên: 11/09/2015, 16:05
Proline rich acidic protein 1 in life and death r6
... Trang 19TNFa production0 1000 2000 3000 4000 5000 6000 7000 10000 U/P 5000 U/P 1000 U/P 500 Tc Tc/U Tc/U 10000 Tc/U/P 5000 Tc/U/P 1000 Tc/U/P 500 Ts Ts/U Ts/U/P 10000 Ts/U/P 5000 Ts/U/P 1000 10000 ... differentiated epithelial cells 4.1.1 Regulation of PRAP1 by differentiation 4.1.1.1 Expression of PRAP1 in intestinal epithelium The proline-rich acidic protein (PRAP1) gene was first identified ... U/P 5000 U/P 1000 U/P 500 Tc Tc/U Tc/U 10000 Tc/U/P 5000 Tc/U/P 1000 Tc/U/P 500 Ts Ts/U Ts/U/P 10000 Ts/U/P 5000 Ts/U/P 1000 Ts/U/P 500 10000 U/P 5000 U/P 1000 U/P 500 Tc Tc/U Tc/U 10000 Tc/U/P
Ngày tải lên: 11/09/2015, 16:05
Molecular function and regulation of the bax associating protein MOAP 1
... INTRODUCTION 1.1 APOPTOSIS 1 1.1.1 Definition and morphology of apoptosis 1 1.1.2 Extrinsic and intrinsic pathways of apoptosis 3 1.1.3 Apoptosis and human diseases 5 1.2 MITOCHONDRIA AS ... stimuli 114 3.2.8 MOAP-1 is a key short-lived protein required for Bax function in mitochondria 117 3.2.9 Conclusions 120 3.2.10 Acknowledgement 121 DISCUSSION 122 4.1 MOAP-1 IS A MITOCHONDRIAL ... FAMILY PROTEINS: LIFE-AND-DEATH SWITCH IN MITOCHONDRIA 14 1.3.1 Discovery of Bcl-2 as an oncogene 14 1.3.2 The Bcl-2 family 16 1.3.2.1 Bcl-2 homolog (BH) domains 16 1.3.2.2 Three classes
Ngày tải lên: 14/09/2015, 12:54
Energy and protein intake, anthropometrics, disease burden and 1 year mortality in elderly home care receivers living in germany
... participants, and more than one quarter of elderly had swallowing problems Daily mean energy intake was 2017±528 in men (n=123) and 1731±451 in women (n=216; p<0.001) Mean protein intake amounted to 1.0 ... chewing problems and almost one third (28%) from swallowing problems Daily mean energy intake was 2017 kcal in men and 1731 kcal in women and mean protein intake amounted to 1.0 g/kg body weight ... elderly Best Pract Res Clin Gastroenterol 2001 Dec;15:869-84 11 Morley JE Anorexia of aging: physiologic and pathologic Am J Clin Nutr 1997 Oct;66:760-73 12 Potter JF, Schafer DF, Bohi RL In-hospital
Ngày tải lên: 19/11/2015, 14:18
Levels and prognostic impact of circulating markers of inflammation, endothelial activation and extracellular matrix remodelling in patients with lung cancer and chronic obstructive
... Alcam (CD166) p < 0.001 Sign p < 0.001 Sign p < 0.001 p < 0.001 ns CD147 p < 0.001 Sign p < 0.001 Sign p < 0.001 p < 0.001 ns DLL1 p < 0.001 Sign p < 0.001 Sign p ... systemic and inhalation), and serum levels of CXCL16 and CD147 (r = − 0.375, p = 0.014 and r = − 0.359, p = 0.020), and a positive correlation between soluble CD14 levels and steroid use (r = 0.419, ... lung carcinoma Cancer. 1998;83(2):276 –82. 9 Durham AL, Adcock IM The relationship between COPD and lung cancer. Lung Cancer 2015;90(2):121 –7. 10 Wiencke JK DNA adduct burden and tobacco carcinogenesis
Ngày tải lên: 03/07/2020, 00:50
Association of rs2282679 A>C polymorphism in vitamin D binding protein gene with colorectal cancer risk and survival: Effect modification by dietary vitamin D intake
... odds ratio was 1.10 (95% CI, 0.88–1.37) per variant C allele and 1.21 (95% CI, 0.70–2.09) in CC homozygotes compared with AA homozygotes The ORs were similar for men and women and did not differ ... were 2.11 (95% CI, 1.29–3.74; P interaction= 0.040), 1.93 (95% CI, 1.08–3.46; P interaction= 0.043), 2.36 (95% CI, 1.26– 4.44; P interaction= 0.004), and 2.03 (95% CI, 1.11–3.72; P interaction= ... Fruit intake (servingsc/wk)b 9.7 ± 8.3 10.9 ± 8.1 0.027 Total vitamin D intake ( μg/d) b Total calcium intake (mg/d)b 1019.8 ± 488.8 1097.1 ± 567.7 0.018 Total dairy products (g/d)b 364.5 ± 303.3
Ngày tải lên: 24/07/2020, 00:33
Stomatin-like protein 2 is overexpressed in epithelial ovarian cancer and predicts poor patient survival
... all patients was 33 and 52 months, respectively The median PFS of patients with high and low/no SLP-2 expression was 19 months (range, 1 ~ 121 months) and 61 months (range, 1 ~ 108 months), respectively ... other cancers, including endometrial cancer, lung cancer, la-ryngeal cancer, and breast cancer [8, 11] Over-expression of SLP-2 in cancer tissues was associated with decreased patient survival and ... 1 Overexpression of SLP-2 mRNA and protein in epithelial ovarian cancer cell lines SLP-2 mRNA and protein expression in epithelial ovarian cancer cell lines (OVCAR3, Anglne, SKOV-3, HO8910, and
Ngày tải lên: 22/09/2020, 23:48
Impact of diabetes type II and chronic inflammation on pancreatic cancer
... analysis of CD133) and incubated overnight at 4°C with a rabbit anti-ALDH1a1 (Cell Signaling, Boston, USA, code 12035, 1:1000), rat anti-CD133 (eBioscience Inc., San Diego, USA, code 14-1331, 1:500) ... article, Trang 2pancreatic cancer of 13.3 and 69.0, respectively [11].Patients with diabetes and obesity have a moderately in-creased relative risk of 1.8 and 1.3 [12,13] These studies indicate ... isolated from obese mice (lean: 1.09/1.06-1.14, n = 3; obese: 1.06/0.96-1.21, n = 6), or 6606PDA cells, which were never injected in any animal (1.05/0.99-1.21, n = 7; median/interquartile range
Ngày tải lên: 30/09/2020, 13:44
Báo cáo khoa học: Identification of the amniotic fluid insulin-like growth factor binding protein-1 phosphorylation sites and propensity to proteolysis of the isoforms docx
... Glu C peptides 83 -10 8 +2P 93 -10 0 +P 93 -10 3 +P 93 -10 3 +2P 93 -10 8 +P 10 0 -11 1 +P 10 9 -12 1 +P 10 9 -12 2 +P 11 2 -12 1 +P 11 3 -12 1 +P 16 1 -17 2 +P Tryptic peptides 16 5 -17 5 +P 16 5 -18 3 +P Sequence Calculated mass ... 16 + 25+ 24+ 22+ 13 + 14 + C 25 410 Da 21+ 20+ 19 + 18 + 23+ 25+ 15 + 17 + 16 + 15 + 13 + 14 + 24+ B 21+ 20+ 19 + 22+ 16 + 18 + 23+ 25+ 17 + 15 + 24+ 14 + 13 + A 10 00 12 00 253 31 Da 14 00 16 00 18 00 252 51 Da 2000 m/z ... 2787 .1 853.4 11 69.4 12 49.4 17 70.7 13 57.6 15 94.7 17 08.7 12 53.5 11 39.4 13 30.0 2787.8 853.2 11 69.3 12 49.2 17 71. 4 13 57.6 15 95.2 17 08.6 12 53.9 11 39.9 13 30.3 +0.7 )0.2 )0 .1 )0.2 )0.3 )0.5 )0 .1 +0.4...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: A zinc finger HIT domain-containing protein, ZNHIT-1, interacts with orphan nuclear hormone receptor Rev-erbb and removes Rev-erbb-induced inhibition of apoCIII transcription potx
... rerio, Drosophila melanogaster and Schizosaccharomyces pombe (GenBank accession nos XP_536855.2, Q8R3 31, NP_0 010 17056.2, NP_0 010 174 01. 1, NP_608895 .1 and NP_595833 .1) Residues identical in all compared ... repression by the FEBS Journal 274 (2007) 5370–53 81 ª 2007 The Authors Journal compilation ª 2007 FEBS 5379 ZNHIT -1 as a cofactor of Rev-erbb 10 11 12 13 14 15 16 J Wang et al orphan steroid receptor Rev-erbb ... dehydrogenase bifunctional enzyme [10 ], a-fetoprotein [11 ] and rat apoAI [12 ] genes Rev-erba is also involved in circadian timing in brain and liver tissue, and regulates Bmal1 which is the master regulator...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Interactions of HIPPI, a molecular partner of Huntingtin interacting protein HIP1, with the specific motif present at the putative promoter sequence of the caspase-1, caspase-8 and caspase-10 genes pdf
... 1. 20E-008 1. 28E-008 1. 00E-008 25 50 75 10 0 0 .10 0 0.095 0.090 0.085 1. 26E-008 AAAGACACG Kd=4 nM 0 .10 5 Kd =1. 2 nM 1/ ΔF340 1/ ΔF340 0 .11 0 Kd=0.29 nM 1. 32E-008 AAAGACATG 1. 40E-008 20 25 30 35 40 45 50 1/ [DNA]µM ... 32.5 ± 1. 8 (13 .5-fold) 23 .1 ± 1. 3 (2.5-fold) 90.2 ± 11 .1 (2.2-fold) 10 .8 ± (7.2-fold) P-values GFP–N-terminus of HIPPI (fold) P-values 0.00 01 12.5 ± 3.9 0.3 0.00 01 0.0002 0.0005 17 .6 ± 1. 9 (7.3-fold) ... Casp1ups 60 bp I C1ups 717 bp (HIPPI-pDED) C1ups 717 bp (HIPPI-Nterm) C1ups 60bp (HIPPI-pDED) 0.00 0. 21 II C1ups 60bp (6XHN-HIPPI_pDED) Kd=0.34nM 0.20 1/ ΔF Fluorescence305 B shifted band 0 .19 0 .18 ...
Ngày tải lên: 07/03/2014, 10:20