... ’-GGAATTCCATATGGATCTTTACAAGAATCCAAATGTAC-3’ Tth xynB3-2 5 ’-CCGCTCGAGCTCGATCTTTGTATTTGTGAAGAAAAC-3’ Trang 9orα-arabinosidase activity after pre-incubation of enzymesat 75°C, 80°C, 85°C, 90°C and ... β-xylosidases from bacteria and archaea Among these families, the members ofβ-xylosidases in GH39, GH52 and GH54 were all from bacteria, and almost no information was available in fungi and archaea ... p-nitrophenyl-β-D-xylopyranoside (pNPX, Sigma, USA) was used for β-xylosidase activity analysis and p-nitrophenyl-α-L-arabionfuranoside (pNPAF, Sigma, USA) forα- arabinosidase activity analysis Under standard assay
Ngày tải lên: 01/11/2022, 08:52
... quasitwo-dimensional electron gas (Q2DEG) in a GaAs/InGaAs/GaAs QW Therefore, the aim of this article is to calculate the finite temperature magnetoresistance of a Q2DEG realized in a GaAs/InGaAs/GaAs lattice-mismatched ... exchange-correlation effects; metal–insulator transition Classification numbers: 71.30.+h; 75.47.Gk c 2020 Vietnam Academy of Science and Technology 124 TRANSPORT PROPERTIES OF A GaAs/InGaAs/GaAs QUANTUM ... temperature transport properties of a quasi-twodimensional electron gas in a GaAs/InGaAs/GaAs quantum well under a magnetic field, taking into account many-body effects via a local-field correction
Ngày tải lên: 17/08/2020, 20:58
Synthesis and X-ray powder diffraction, electrochemical, and genotoxic properties of a new azo-Schiff base and its metal complexes
... plate-incorporation assay was examined with Salmonella typhimurium TA98 and TA100 strains in the presence and absence of S9 mix according to Maron and Ames.30 Mutagenicity tests and preparation of S9 ... elemental analysis, and infrared and ultraviolet-visible spectroscopy; the proton NMR spectrum of the mbH ligand was also recorded The azo-azomethine ligand, mbH, behaves as a bidentate ligand coordinating ... mutagens Each strain used for testing was checked for the presence of strain-specific marker as described by Maron and Ames.30 2.9.2 Mutagenicity assay and preparation of S9 The standard plate-incorporation
Ngày tải lên: 12/01/2022, 23:04
influence of dust and mud on the optical chemical and mechanical properties of a pv protective glass
... glass, dust, dry mud, and glass surface after mud removal. Trang 7small particles The alkali and alkaline earth metals (Na and Ca) in the particles dissolve in the water during the mud formation, ... Trang 1Influence of dust and mud on the optical, chemical, and mechanical properties of a pv protective glass Bekir Sami Yilbas 1 , Haider Ali 1 , Mazen M Khaled 2 , Nasser Al-Aqeeli 1 , Numan ... Sahara Desert was investigated by Middleton5 The data showed that dust-storm activity in the west and east of the Sudano–Sahelian belt had dramatically increased during the drought years; by a
Ngày tải lên: 04/12/2022, 14:53
Surface reconstructions and related local properties of a bifeo3 thin film
... centers of mass of the surrounding four Bi atoms The statistical error bars in the matrix are smaller because of the larger amount of data Trang 6a type I surface A closer look at the averaged ABF ... study of such surface phenomena is of great importance to gain a basic understanding of physical interactions between lattices, charges and polarizations at surfaces or interfaces in BFO, which may ... lattice constant along the x- and y- axes and fixed them to the calculated lattice constant of DSO (110)o We then scanned along the z-axis and fully relaxed all of the atoms to obtain a local energy
Ngày tải lên: 19/03/2023, 15:41
Modelling of dynamical and statical properties of a car seat with adjustable pressure profile
... scanning of surface of the additional latex tube Figure 3.35 Example of scanned surface of the additional latex tube The data is illustrated as a cloud of points on the surface of the additional ... chapter 2, 3 and 4 First, the structure and function of the car seat using the device which was made in the past is presented and analyzed carefully in chapter 2 Chapter 3 presents analytical and ... results and experimental results Simulations were conducted with internal pressures maintained at constant values of 0 kPa, 5 kPa, 10 kPa, 15 kPa, 20 kPa, and 25 kPa For each internal pressure scenario,
Ngày tải lên: 16/11/2023, 14:01
effect of normalizing temperature on microstructure and mechanical properties of a nb v microalloyed large forging steel
... conditions in this paper, the formation of austenite in initial ferrite and pearlite was asynchronous The nucleation and growth of austenite in initial pearlite was earlier and faster than that in ferrite ... typical TEM micrographs and EDS analysis of the precipitations (a and d)cuboidal precipitation, (b and e)spherical precipitations, (c and f)fine precipitations Fig 12 Three typical TEM micrographs ... samples was etched by a 4% nital solution The size and area fraction of ferrite and pearlite constituent were measured by software Image-Pro Plus For each specimen, at least 5 fields of view containing
Ngày tải lên: 30/04/2024, 21:42
Campaigns of a Non-Combatant, and His Romaunt Abroad During the War ppt
... doubtthat I shall hear of him again as the proprietor of an overland mail, or the patron and capitalist of Greenlandemigration I passed the second and third days quietly in camp, writing a couple of ... horse, a deep challenge came directly from the thicket, and I saw the flash of a sabre, as Istammered a reply Led to a cabin close at hand, my pass was examined by candle-light, and I learned that ... into a bouncing amble and left the attaché far behind My pass was again demanded above Langley's by a man who ate apples as he examined it, and who was disposed to hold a long parley I entered a
Ngày tải lên: 07/03/2014, 01:20
Hollywood on the Head of a Pin: Montage and Marketing at the Oscars® docx
... the era of the global popular film market, is also a magic word At once a place in Los Angeles, an industrial marker and a mythical land, it is emblematic of contemporary cinema’s contradictory ... media complex and mall at Hollywood and Highland offered— for virtual guests—geographic cachet, ersatz Intolerance elephants, and big-screen video displays As commentators and stars alike remarked, ... what Workman’s Oscar montages tend to communicate is a sort of cult of the cut, ultimately naturalizing quantity (the fast-paced abundance of images) as quality (by, moreover, an Academy-Award
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Studies on the regulatory properties of the pterin cofactor and dopamine bound at the active site of human phenylalanine hydroxylase pptx
... Trang 1Studies on the regulatory properties of the pterin cofactorand dopamine bound at the active site of human phenylalanine hydroxylase Therese Solstad1, Anne J Stokka1, Ole A Andersen2and ... structure of the hPAHÆadrenaline/ dopamine binary complex [17] was superimposed onto that of the ligand-free rPAH containing the regulatory and catalytic domains [19] the catecholamine main-chain is also ... binding of substrate slightly increases the rate of phos-phorylation of rPAH and hPAH by PKA, H4biopterin acts as a negative effector on the same process [12,13].Finally, nonenzymatic deamidation of
Ngày tải lên: 17/03/2014, 09:20
Báo cáo khoa học: Cleavage site analysis of a serralysin-like protease, PrtA, from an insect pathogen Photorhabdus luminescens and development of a highly sensitive and specific substrate pdf
... metallo-endoprotease of Serratia marcescens(serralysin), are the alkaline protei-nase of Pseudomonas aeruginosa, the ZapA metallo-protease of Proteus mirabilis and proetases A, B, C, G and W of various ... internal standard, because it was not hydrolysed by PrtA Peak areas were normalized with the internal standard, and the degree of cleavage was calculated from the reduction in the normalized area of ... of such a substrate based on analysis of PrtA cleavage site specificity, and kinetic characterization of PrtA activity on the new substrate Results and Discussion Identification of PrtA cleavage
Ngày tải lên: 23/03/2014, 09:20
Báo cáo khoa học: Dynamics of a-synuclein aggregation and inhibition of pore-like oligomer development by b-synuclein doc
... with biochemical and ultrastructural analysis, that a-syn can arrange into homodimers that can adopt nonpropagating and propagating conformations The evidence predicts that propagating a-syn dimers ... nonpropagating (head-to-tail) and propagating (head-to-head) conformations Propagating a-syn dimers on the membrane incorporate additional a-syn molecules, leading to the formation of pentamers and ... a-syn is a 140-amino acid (aa) protein, and b-syn is a 134-aa protein Each of the synucleins is composed of an N-terminal lipid-binding domain containing 11 residue repeats and a C-terminal acidic
Ngày tải lên: 23/03/2014, 09:21
Báo cáo khoa học: Odorant binding protein has the biochemical properties of a scavenger for 4-hydroxy-2-nonenal in mammalian nasal mucosa doc
... tracheobronchi- al tree, nasal mucosa and sweat glands [25]. Human tear lipocalin has significant sequence homology with the human forms of OBP and, at least in humans, par- tially shares a similar tissue ... functional properties of this form (in terms of aggregation state and binding with different ligands including AMA, HNE and unde- canal) are analogous to those of the native form (data not shown). The ... radical intermediates generated as a consequence of oxidative metabolism. They can react with virtually all classes of biological molecules and are responsible for most of the cellular damage caused by oxidative
Ngày tải lên: 23/03/2014, 10:20
Báo cáo khoa học: Characterization of a-synuclein aggregation and synergistic toxicity with protein tau in yeast potx
... extraneuronal plaques composed of aggregated amyloid (Ab) peptides and by intracellular paired helical filaments and neuro-fibrillary tangles In the absence of b-amyloid, paired helical filaments and neurofibrillary ... a-synuclein aggrega-tion and cytotoxicity, and offer great potential for defining the underlying mechanisms of toxicity and synergistic actions of a-synuclein and protein tau Abbreviations AD, Alzheimer’s ... conformational changes in protein tau appear to be essential in the develop-ment of tau pathology [14–16] Considerable overlap in the pathology of AD and PD has been reported For instance, tau and a-synu-clein
Ngày tải lên: 23/03/2014, 13:20
Báo cáo khoa học: Crystal structure determination and inhibition studies of a novel xylanase and a-amylase inhibitor protein (XAIP) from Scadoxus multiflorus pot
... nucleotide and derived amino acid sequences of XAIP are available in the EMBL/GenBank/DDBJ databases under the accession number EU663621 Structural data are available in the Protein Data Bank database ... molecular weight of the ternary complex of XAIP, GH11 xylanase and GH13 a-amy- lase. These observations indicate that XAIP associates with GH11 xylanase and GH13 a-amylase, as well as with both xylanase ... xylanase was recorded to be up to 50% for an enzyme to XAIP molar ratio of 1 : 1.5 (Fig. 3B). Similarly, at a molar ratio of 1 : 1.2 between a-amylase and XAIP, the activity of a-amylase was reduced
Ngày tải lên: 29/03/2014, 09:20
Báo cáo khoa học: Protein dissection enhances the amyloidogenic properties of a-lactalbumin potx
... USA) All other chemi-cals were of analytical reagent grade and were obtained from Sigma or Fluka (Basel, Switzerland) Trang 10Isolation of nicked and gapped LAThe LA derivatives were obtained ... 1000 LA Th1-LA pH 2.0 0 10 20 30 40 50 LA Th1-LA desβ-LA pH 7.4 Fig 2 Conformational characterization of Th1-LA and desb-LA by CD (A, B) and ANS binding (C, D) Far-UV CD spectra of Th1-LA and desb-LA ... rearrangement takes place during the aggregation process, as given by the disap-pearance of the a-helix bands at 208 and at 220 nm and by the development of the band near 217 nm, typ-ical of b-secondary
Ngày tải lên: 30/03/2014, 16:20
Tài liệu Báo cáo Y học: Antibacterial and antifungal properties of a-helical, cationic peptides in the venom of scorpions from southern Africa pptx
... explain the higher antibacterial activity on Gram-negative bacteria for parabutoporin compared to opistoporin Also with magainin analogs, an increase in antibacterial activity against Gram-negative ... being least active against S marcescens Mastoparan is far less active in inhibiting growth of Gram-negative bacteria and melittin is only active against three of the Gram-negative bacteria at the ... (34 amino acids) [19], peptides consisting of amino acids 7–22 of parabutoporin (an a- helical part having the four amino acids LAKK identical to mastoparan) and of the first 28 amino acids of the...
Ngày tải lên: 21/02/2014, 15:20
Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf
... were made with PYMOL [47] able sequences, some bacterial family 19 chitinases have catalytic domains that are at least as large as those of the plant enzymes and that may contain at least six ... Haemophilus influenzae, and Pseudomonas aeruginosa Although many plant family 19 chitinases are known, crystal structures are available for only two of these [9,15–17] The first structure of a ... 4-MU-(GlcNAc)3, as also earlier demonstrated by Saito et al [21] On the other hand, ChiG showed activity against a- chitin and b-chitin (supplementary Fig S2), chitooligosaccharides (Figs and 3), carboxymethyl-chitin–remazol–brilliant...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Dissociation/association properties of a dodecameric cyclomaltodextrinase Effects of pH and salt concentration on the oligomeric state pot
... mutant, 5Â-TCTGCTGCAGCA GGGTGTT GAGAAGCGCTGGATG-3Â (forward) and 5Â-CATCCAG CGCTTCTCAACACCCT GCTGCAGCAGA-3Â (reverse); for the H539V mutant, 5Â-CGACAAGGCGGGCGTC ACGTTA ACGCTGCCTGTCC-3Â (forward) ... His89 to 118 Val89, and His539 to Val539 using the following primers: for the H49V mutant, 5Â-AGTACATGTGGGACGTCAC CATGGAGTATGTCCC-3Â (forward) and 5Â-GGGACAT ACTC CATGGTGACGTCCCACATGTACT-3Â (reverse); ... dissociation of dodecameric CDase I-5, sedimentation equilibrium 110 Fig Apparent molecular mass of CDase I-5 at various pH values determined by analytical ultracentrifugation analysis analysis was performed...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Purification and properties of a new S-adenosyl-Lmethionine:flavonoid 4¢-O-methyltransferase from carnation (Dianthus caryophyllus L.) pot
... was a mixture of 0.05 M NaPi buffer, pH 3, and acetonitrile (6 : 1, v/v); separation was performed isocratically, at a flow rate of mLÆmin)1, and the volume of injected samples was 10 lL The amounts ... B and eluted with 80 mL of a 0–0.3 M linear gradient of NaCl in buffer B, at a flow rate of 0.4 mLÆmin)1 Fractions containing an F 4¢-OMT activity were pooled, dialyzed and concentrated as above ... of molecular mass markers are indicated in kDa F 4¢-OMT activity towards the different assayed substrates, transformation products and kinetic analysis The enzyme became inactive when the assayed...
Ngày tải lên: 08/03/2014, 08:20