promoter and tissue specific expression of recql4

... a-amylase and its tissue- specific expression in adult midgut; hence, in the present paper the geographic variation of these types of polymorphism is presented MATERIALS AND METHODS An analysis of tissue- specific ... regions of the posterior (PNIG) midgut The presence of activity is indicated by a number and absence by zero (eg, 123 10 means activity in the regions of AMG and region of PMG, but absence of activity ... frequency of 5% and higher, in some populations and MAP-types (123 00, 120 12, 100 12), which occurred only in a number of populations with a frequency of 10% and higher As the genotypes of different...

Ngày tải lên: 14/08/2014, 20:20

... glucocorticoid metabolism and signalling Site -specific variations of human fibroblastic 11β-HSD1 expression versus other components of glucocorticoid metabolism and signalling Expression of mRNA for (a) ... role in tissue- specific and inflammation -specific regulation of fibroblast function Page of 10 (page number not for citation purposes) Arthritis Research & Therapy Vol No Hardy et al Figure Expression ... as interferon-γ and IL-4 and had no significant effect on 11β-HSD1 expression in any of the fibroblasts The site -specific differences in fibroblastic 11β-HSD1 mRNA expression and differential...

Ngày tải lên: 09/08/2014, 08:22

... locus and the variability of tissue- specific expression [9] The latter type of variability is represented by the number and position of the amylase activity regions in the midgut [5] Inter- and ... the level s F of tissue- specific expression, as reflected in the number of active midgut regions and the specific activity of amylase in Drosophila subobscura adults MATERIALS AND METHODS Drosophila ... of the enzyme specific activity of either genotype (H 5.727, P > 0.05) Analyses of the association between the number of active regions and the enzyme -specific activity through compensation of...

Ngày tải lên: 09/08/2014, 18:21

... changes and cell -specific or tissue- specific gene expression We focused on the tissue- specific response to stress in detail by including a dataset in which cell typespecific and growth stage -specific ... clusters N14 and N18 included mainly genes related to the key machineries of gene expression and protein synthesis, most of which organized in tissue cluster T3 (stage specific) and T7 (stages and 2) ... of data, we analyzed in context the genome-wide expression profiles modulated by a number of stress conditions, regulatory cis-elements in promoters, and cell -specific and developmental age-specific...

Ngày tải lên: 14/08/2014, 20:22

... formation of a, b, c, d, e, f and g isoforms) [8–12]; (b) the use of an extrinsic promoter (P1) and an alternative, intrinsic promoter (P2) in the 5¢-end (leading to the formation of TA and DM classes ... TA isoform(s) and the antiapoptotic DM isoforms In general, TAp73 isoforms regulate the transcription of DMp73 isoforms, which, in turn, act as dominant negative regulators of both TAp73 and p53, ... identification of novel transcriptional Sp1 activates p73 P1 promoter in lung cancer factors that control the use of the p73 P1 promoter and, subsequently, the relative expression of p73 isoforms in...

Ngày tải lên: 29/03/2014, 09:20

... range of human and rat tendons and ligaments in terms of their progenitor cell population, responsiveness to dexamethasone, and their expression of cell surface markers Methods Reagents and consumables ... in tissue engineering, in particular of mesenchymal tissues, holds great promise for the treatment of chronic and debilitating diseases However, the source of cells for the generation of tissue ... progenitor cells and response to dexamethasone Cells from pairs of PT and ACLs were examined for their levels of progenitor cells and their response to a range of concentrations of dexamethasone...

Ngày tải lên: 20/06/2014, 01:20

... Page of 10 Figure Ca-HGO expression in the leaf and stem of 3-month-old Camptotheca acuminata plantlets Paraffin-embedded cross sections of leaves (A and B) and primary body of the stem (C and ... compared with the expression pattern of Ca-TDC1, Ca-TDC2, and Ca-HGO genes, which are involved in TIA biosynthesis Both the accumulation of CPT and the expression of Ca-TDC and Ca-HGO genes at ... of the early stages of CPT biosynthesis at the cellular level and determine whether these sites are the same as those of CPT accumulation, the cellspecific localization of Ca-TDC and Ca-HGO expression...

Ngày tải lên: 12/08/2014, 03:21

...   ¡ ¢   ¡ ¡     ( teristic profile of a bona fide syncytin gene, with high levels of expression in the placenta and very limited expression in other tissues Expression in the placenta decreases ... Transcription profile and identification of a placentaspecific envelope Quantitative RT-PCR analysis of transcript levels for the two identified candidate syncytins was performed using primers specific ... layers of the of a rabFigure indicated Structure and in situ hybridization for syncytin-Ory1 expression of day 12 rabbit placenta: (A) Schematic representation of a rabbit placenta (right) and haematoxylin...

Ngày tải lên: 12/08/2014, 23:22

... as well as seven of eight in Group had the expected bands (Figure 3) Brain -specific ERVmch8 expression We then examined the expression pattern of ERVmch8 in a set of 16 selected tissues from female ... series of potential 11 short-term and long-term events: 1) persistent expression of gag, pol, and env polypeptides, and their potential contribution to the biology of the cerebellum, 2) assembly of ... Start sites of U3 and R are indicated with an arrow Plus strand cytosine residues of the ERVmch8 (minus strand of the chromosome orientation) are indicated by upward circles and minus strand with...

Ngày tải lên: 13/08/2014, 01:21

... treatment of sepsis and associated diseases Because of this, it is of interest to determine molecular mechanisms and functions of DHEA treatment We therefore investigated the effects of DHEA application ... 11.05 ± 1.94 Figure TNF-α expression (a) In liver after 48 and 96 hours Relative mRNA expression expression of TNF-α in liver tissue, detected by semi-quantitative RTPCR 48 and 96 hours after the ... expressed as mean ± standard error of the mean (b) In lung after 48 and 96 hours Relative mRNA expression of TNF-α in lung tissue, detected by semi-quantitative RT-PCR 48 and 96 hours after the...

Ngày tải lên: 13/08/2014, 18:22

... intensity of the ethidium bromidestained band with the marker bands A total of 30–90 ng of DNA is recommended for direct sequencing of PCR products Analysis, sequencing and in vitro expression of PCR ... primers Of course in this case the use of an enzyme with 3′-exonuclease proofreading activity should be avoided to protect the differentiating 3′-end An example of the design of original and nested ... giving a clear result and is relatively rapid and simple requiring mixing of an aliquot of PCR product, 10× restriction buffer and restriction enzyme, incubation to allow digestion and then agarose...

Ngày tải lên: 25/10/2013, 22:20

... variety of tissues.[16, 17, 22, 23] This study examined the expression of TLR2, TLR4, TLR5, and TLR9 in normal primary human professional phagocytes Constitutive expression of TLR2, TLR4, and TLR9 ... increased expression of TLR2 and decreased expression of TLR5 TLR2 was significantly increased by 740 ± 180 % TLR4 and TLR9 levels were not affected by GM-CSF (Figure 3) Monocyte TLR2 and TLR5 expression ... incubation, expression of TLR2 and TLR4 declined in neutrophils maintained in culture in the absence of cytokine supplementation Specifically, TLR2 and TLR4 expression decreased by 76 ± 9.5 % and 78...

Ngày tải lên: 08/08/2014, 16:23

... among these groups Tissue distribution of IL-18 expression and identification of IL-18-producing cells in salivary glands of patients with SS and chronic sialoadenitis IL-18 expression in inflammatory ... histological evaluation of the number of foci, the presence of IL-18 in inflammatory foci and ducts, the degree of organization of the mononuclear aggregates, and the presence of germinal centers ... enhance expression of costimulatory molecules such as CD40L and CD86 [16,17], and to induce tissue damage through the induction of cell-mediated cytotoxicity [18-21] and the release of matrix...

Ngày tải lên: 09/08/2014, 01:23

... Care and Use Committee of National Chung Hsing University and of the Human Nutrition Research Center on Aging at Tufts University Induction of arthritis After the washout period, animals of the ... folate, and vitamin B12 Blood was also collected for hematology and chemistry analyses Each patient's blood specimens were kept on ice and were centrifuged within 15 of the blood draw Animal model of ... directly decreases the primary pools of vitamin B6 metabolism and storage, and to examine whether inflammation alters the excretion of vitamin B6 in vivo Materials and methods Clinical trial Study...

Ngày tải lên: 09/08/2014, 07:20

... alignment and mapping steps of the MMSEQ workflow Table MMSEQ estimates for H13 and Mcts2 isoforms in F1 hybrid samples MMSEQ estimates for each haplotype and isoform of H13 and Mcts2 of the initial and ... the expression of the two versions of each heterozygote-containing isoform (’haplo-isoform’) individually and thus it can detect asymmetric imbalances between isoforms of the same gene Our software ... inversion of the observed information matrix When analyzing the expression of thousands of transcripts, the high dimensionality of the observed information matrix and the possibility of identical...

Ngày tải lên: 09/08/2014, 22:23

... evaluation of the impact of CSOM on QOL (question 14) and one question to indicate the frequency of doctor visits in the last six months as a result of CSOM (question 15) are asked The total score and ... http://www.hqlo.com/content/9/1/48 Page of Table Results for the scales of COMOT-15 and SF-36 at three different times of assessment; M = mean value, SD = standard deviation, TM = time of measurement, p = p-value ... Health-related quality of life (HR-QOL) has an everincreasing importance as an outcome parameter For the proof of the success of surgical interventions, the evidence of an improvement of HR-QOL in addition...

Ngày tải lên: 12/08/2014, 01:22

... Secretion of IL-8 and CCL20 and Induction of cell surface ICAM-1 expression in A549 cells cocultured with MT-2 cells Secretion of IL-8 and CCL20 and Induction of cell surface ICAM-1 expression ... http://www.retrovirology.com/content/5/1/86 Activation of NF-κB and AP-1, and viral promoter LTR in HTLV-I-infected lung epithelial cells Tax activates the expression of various cellular genes through the NF-κB and AP-1 pathways ... specimens of A549 and NCI-H292 cells at and days of culture contained no viable MT-2 cells As shown in Figure 1, A549 and NCIH292 cells cocultured with MT-2 cells showed strong expression of Tax...

Ngày tải lên: 13/08/2014, 05:21

... induction of SOCS and both the presence of sepsis and the administration of PN [14] Administration of 16 hours of PN was associated with induction of the expression of hepatic mRNA of the SOCS ... concentrations of a pleiotropic cytokine (IL-6) and an anti-inflammatory (IL-10) cytokine and the expression of mRNA of SOCS proteins, of IGF-I and of the growth hormone receptor (GHR) in hepatic tissue ... found that nutrition and the route of nutrition in sepsis differentially influence circulating cytokine profiles and the expression of mRNA of SOCS proteins, of the GHR and of IGF-I The present...

Ngày tải lên: 13/08/2014, 08:20

... Science and Engineering, the Superintendent of the Postgraduate School, and the President of the College of Naval Architecture and Power Now, he is the Direct, Institute of Thermal Science and Power ... a p and bv must change synchronously The results show that the maximum work output and the maximum efficiency of the cycle will decrease with the increases of a p and bv , and the values of compression ... simulation of performance of an Otto cycle with heat transfer and variable specific heats of working fluid Int J Therm Sci., 2005, 44(5):506-511 [21] Ge Y, Chen L, Sun F, Wu C The effects of variable specific...

Ngày tải lên: 09/09/2015, 10:17

... tissues Relative mRNA expression for 17 and 22 transcripts was assayed in drought-stressed and well-watered berry tissue and leaf tissue, respectively A linear regression of the log2-ratios of ... under-counting of abundant transcripts and over-counting of rare ones, they violate the assumption of random sampling, and as such, are not usually considered for use in tag frequency analyses of gene expression ... generated and analyzed to identify gene expression related to a wide range of processes including berry development in wine grape [30,45] and in table grape [46], tissue- specific gene expression...

Ngày tải lên: 11/08/2014, 11:20