plasma cell proliferative disorders

... Additional file 1 T cell counts (per l) HIV-1 viral load (log copies/ml) HIV-specific CD4+ T cells (p24 proliferation- stimulation index) HIV-specific CD8+ T cells (IFNJ spot-forming cells/10 6 PBMC) ... controlling viraemia, these must also be accompanied by p24-specific T cell proliferative responses [23-25] Appropriate T cell help is also required to achieve maturation and display of effector ... stable CD4 T cell counts >500 cells/μl, and no history of ART [27,28] Subsequently, loss of LTNP status was defined by any of the following events: a consistent decline in CD4 T cell counts

Ngày tải lên: 13/08/2014, 05:21

... circulating cell-free DNA (cfDNA), which includes both tumor-derived DNA (so-called “circulating tumor DNA”, or ctDNA) and DNA derived from non-tumor cells, such as hematopoietic and stromal cells, ... blood There is growing evidence of interactions between cancerous cells and other cells, including fibroblasts, platelets, and immune cells, espe-cially within the tumor microenvironment These in-clude ... AccessMachine learning enables detection of early-stage colorectal cancer by whole-genome sequencing of plasma cell-free DNA Nathan Wan†, David Weinberg†, Tzu-Yu Liu, Katherine Niehaus, Eric A Ariazi, Daniel

Ngày tải lên: 17/06/2020, 17:20

... related but not synonymous Transferrin is the sole plasmaprotein that binds iron The TIBC therefore depends on the quantity of transferrin in the plasma A mathematical conversion is needed to directly ... andserum iron arises from the fact that ferritin is the storehouse for intracellular iron.30Ferritin molecules within cells are multi-subunit spherical shells that can sequestermore than 4000 iron ... ferritin is thesame as intracellular ferritin and consequently transports iron in the serum Serum ferritin is a secreted protein that contains essentially no iron.31 Cellular iron storesmodulate

Ngày tải lên: 10/08/2014, 08:20

... excess of 10,000 cells/μL reduce the risk of catastrophic bleeds, such as intracranial hemorrhage 䊏 PURE RED CELL APLASIA Pure red cell aplasia reflects selective destruction of red cell progenitors ... pure red cell aplasia Absentfrom the peripheral blood are abnormal red cell features such as schistocytes, targetcells, spherocytes, or basophilic stippling The peripheral blood red cells are ... heterogeneous conglomeration of disorders derived from a collection ofimpendent mutations involving genes on several chromosomes.1Cell fusion studiesdone in vitro show that cellular components from

Ngày tải lên: 10/08/2014, 08:20

... patterns in these disorders First, independent mitochondrial replication com-bined with random segregation into the daughter cells at cell division means that bypure chance newly produced cells can have ... nuclear genome When cells undergomitosis, the organelles distribute randomly to the daughter cells Acquired mitochon-drial defects therefore pass unevenly to the daughter cells.130This property ... ATP produced by eukaryotic cells The mature erythrocyte is the sole mammalian cell devoid ofmitochondria, with consequent total reliance on glycolysis for energy Most cellscontain between 100 and

Ngày tải lên: 10/08/2014, 08:20

... membranestructures produce rigid red cells with impaired ability to pass through capillaries Trang 14FIGURE 15–2 Heinz body prep of G6PD deficient cells The red cells are ghostly outlines with the ... decrepit red cells Disturbed ionchannel activity produces erythrocyte swelling and rupture Some cells show bizarredistributions of hemoglobin with protein confined to one portion of the cell and aghostly ... Reticuloendothelial cells rapidly remove these spavined erythrocytes from thecirculation Sometimes an erythrocyte rips free of the reticuloendothelial cell, leaving a large rent in its membrane The cells appear

Ngày tải lên: 10/08/2014, 08:20

... developing erythroid precursor cells Cellular immunity can damage ordestroy these cells as well The result is a singular process with very limited produc-tion of red cells by an erythron that generates ... begins with a thor-ough washing of the cells to be tested in order to remove any adventitial plasmaproteins that might adhere to the cell membrane The only human plasma componentleft to react with ... VIIIMaternal Circulation Antibody 4 Antibody 3 Endogenous Red Cells Transfused Red Cells Antibody 2 Lymph Node Antibody 1 Normoblast Packed Red Cells FIGURE 19–1 Schematic representation of the components

Ngày tải lên: 10/08/2014, 08:20

... binding Cell lines and patient-derived primary lymphocytes The acute lymphoblastic leukemia (WSU-pre-B-ALL), dif-fuse large cell lymphoma cell line (WSU-DLCL2), follicu-lar small cleaved cell lymphoma ... cytotoxic effect on primary lymphoma cells The CLL/SLL cells expressed CD5, CD19, CD20 and faint monotypic SIg The MZL cells were CD5-, CD19+ and CD20+ Mononuclear cells were separated by Ficoll-Hypaque ... washed twice with PBS and then cell pellet was resus-pended in RPMI-1640 culture medium Effect of TW-37 on Growth of established cell lines and fresh lymphoma cells Cells from established lines

Ngày tải lên: 10/08/2014, 22:20

... species, cell wall fortification and a special form of programmed cell death (PCD), also known as hypersensitive cell death [3-5] PCD is an important mechanism of removing unwanted cells in order ... whose members function in cell proliferation, ion channel reg-ulation and cell death [15] HIR protein expression in maize and barley is associated with localized host cell death and disease resistance ... co-localization of OsHIR1 (15 nm gold particles) and OsLRR1 (6 nm gold particles) to the plasma membrane PM: Plasma membrane; CW: Cell wall. Trang 5OsHIR1 were generated Three weeks after germination,the leaves

Ngày tải lên: 11/08/2014, 11:21

... University Cell and DNA Repository (which contains the AGRE samples), cultures were split 1:2 every 3 to 4 days, and cells were harvested for miRNA isolation 3 days after a split, while the cell lines ... to known genetic disorders, such as fragile X syndrome, tuberous sclerosis complex, and Rett syndrome [2,3] However, the etiology of ASD in Abstract Background: Autism spectrum disorders (ASD) ... post-transcriptional gene regulatory networks associated with autism spectrum disorders by microRNA expression profi ling of lymphoblastoid cell lines Tewarit Sarachana1, Rulun Zhou2, Guang Chen2, Husseini

Ngày tải lên: 11/08/2014, 12:20

... con-centration exposed to the cells n = 5 Trang 7Electron micrographs of the apical plasma membrane of A549 cellsFigure 4 Electron micrographs of the apical plasma membrane of A549 cells A: Control experiments ... Mini Kit (Qiagen AG, Basel, Switzerland) The cells were released from the cell culture membrane with a cell scratcher and the provided lysis buffer The cell lysate was then centri-fuged in shredder ... into the cells Qualitatively, differences in cellular uptake were observed between the differently sized particles The majority of 1 Surface area of the apical plasma membrane of A549 cells at

Ngày tải lên: 12/08/2014, 14:20

... sample to measure cell-free plasma DNA was taken from the antecubital vein of each patient immediately after return of spontaneous circulation in the emergency room Plasma and cells were separated ... minutes and plasma samples were stored at -80°C Plasma samples were centrifuged at 16,000 g for 10 minutes before DNA extraction to remove any residual cells DNA was extracted from 200-μl plasma samples ... the cell-free circulating plasma DNA measurements by residual white blood cells or platelets we used high-speed centrifugation at 16,000 g after storage, which almost com-pletely eliminates cellular

Ngày tải lên: 13/08/2014, 20:21

... inner cell mass forms all three germ layers of the embryo The cells of the inner cell mass, known as embryonic stem cells, can be extracted and cultured in vitro, and are able to generate any cell ... aberrations in cultivated stem cells and their progeny 91 5.6 ADK-/- lt-NES cells as an adenosine releasing cell population 92 5.7 The effect of grafted ADK-/- lt-NES cells in epileptic animals ... 1.1 Stem cells Stem cells have the remarkable potential to differentiate into specialized cells and thereby are key factors for the development of the whole organism All types of stem cells share

Ngày tải lên: 26/11/2015, 09:54

... Adipose tissue-derived stem cells, Articular cartilage injury, Joint failure, Mesenchymal stem cells, Osteoarthritis, Platelet-rich plasma Introduction Platelet-rich plasma (PRP) has been widely ... Stem cells from adipose tissue were isolated and differenti-ated in vitro into adipogenic, chondrogenic, myogenic, and osteogenic cells in the presence of specific induction factors [12] These cells ... resuspended in PBS to determine the cell quantity and viability using an automatic cell counter (NucleoCounter; Chemometec, Gydevang 43, DK-3450 Allerod, Denmark) Platelet-rich plasma preparation Human

Ngày tải lên: 12/12/2017, 05:46

... Hematopoietic stem cell transplantation B-thalassemia major Fanconi’s anemia Immunodeficiency diseases Inherited metabolic disorders A B S T R A C T Allogeneic hematopoietic stem cell transplantation ... Trang 1ORIGINAL ARTICLEAllogeneic hematopoietic stem cell transplantation for non-malignant hematological disorders a Department of Medical Oncology, National Cancer Institute, ... whole group of BTM patients was 72.4% DFS was 74% for peripheral blood stem cell (PBSC) group compared to 64% in the BM stem cell group The inci-dence of graft rejection was significantly lower in

Ngày tải lên: 15/01/2020, 06:21

... source of RNA than whole plasma, while other miRNAs show better diagnostic performance when tested in the whole plasma Keywords: Prostate cancer, Cell-free miRNAs, Extracellular vesicles, Exosomes, ... cell-free miRNA present in plasma was recovered from EVs, the EV-incorporated miRNA profile is clearly dif-ferent from that in the whole plasma Diagnostic potential of EV-enclosed and total cell-free ... RNA profiles in EVs, plasma and serum of 3 healthy individuals and showed that the miRNA levels dif-fer remarkably between plasma and serum EVs and between EVs and cell-free plasma and serum [30]

Ngày tải lên: 06/08/2020, 04:44

... readout The continuous monitoring of cell viability by the xCELLigence system allows us to distinguish between cell death and reduced proliferation [29] Cells (5 × 103 cells/well) were seeded into E-plates ... distribution of cells in each cell cycle phase: sub-G1 (dead cells), G1, S Trang 5and G2/M Results were displayed as the average ofthree separate experiments Real-time proliferation analysis Cell proliferation ... in cell lines revealed that induction of the NGF-TrkA signaling produced a phenotype of dramatic suppression of cell proliferation through inhibition of cell division and pronounced intracellular

Ngày tải lên: 23/09/2020, 00:06

... A R C H A R T I C L E Open AccessPrognostic information of serial plasma osteopontin measurement in radiotherapy of non-small-cell lung cancer Christian Ostheimer*, Matthias Bache, Antje Güttler, ... prognostic effect of baseline OPN plasma levels which was increased by co-detection with other hypoxia-related proteins in the radical radiotherapy of non-small-cell lung cancer (NSCLC) This prospective ... radiotherapy of NSCLC Keywords: Osteopontin, Radiotherapy, Non-small-cell lung cancer, Tumor hypoxia, Prognostic factors, Plasma biomarker * Correspondence: Christian.Ostheimer@uk-halle.de Department

Ngày tải lên: 30/09/2020, 15:07

... produced predominantly by monocytes, macrophages, and dendritic cells, and is a potent chemoattractant for macrophages, NK cells, and some T cells [36] It is appar-ent that the levels of CCL22 mRNA ... non/ex-smokers (nonsmokers and COPD ex-smokers), plasma CXCL8, CCL22, CCL4, and MVD were greater in current smokers No differences in other cytokines were seen Plasma CXCL8 moderately correlated with ... correlates significantly with the development of goblet cell hyperplasia Keywords: Mucin, Chronic obstructive pulmonary disease, Goblet cell hyperplasia, Chemokine, Neutrophil, Macrophage * Correspondence:

Ngày tải lên: 23/10/2022, 16:56

... Introduction Plasma medicine and plasma’s biomedical applications are attracting rapidly increasing levels of interest in plasma research [1, 2] The fields of application include plasma-based ... pressure plasmas for biomedical applications Currently, however, there is very little fundamental knowledge regarding the relevant interaction mechanisms of plasma species with living cells In ... of reactive oxygen plasma species interacting with bacterial cell walls M Yusupov1,3, E C Neyts1, U Khalilov1, R Snoeckx1, A C T van Duin2 and A Bogaerts1 1Research Group PLASMANT, Department

Ngày tải lên: 02/11/2022, 08:50