pki x 509 certificate and crl extensions profile 30 4 2002

... level expression of peptides and proteins using cytochrome b5 as a fusion host Protein Expr Purif 41 , 84 97 46 Scopes RK (19 94) Protein Purification, Principles and Practice Springer, New York 47 ... maps and facilitate further rebuilding and improvement of the molecular model, until no unexplained electron density remained, and the Rcryst and Rfree values converged at 18.9% and 24. 4%, respectively ... backbone and side chain 13Ca and 13C¢ and 13Cb carbons are assigned to the extent of 97%, 93% and 93%, respectively (BMRB accession number 6 940 ) [ 24] The H–15N correlation in the TROSY experiment...

Ngày tải lên: 16/02/2014, 09:20

... 100%; H6b-e, 48 .4% and 36.6% (two non-equivalent protons), determined from 1D STD NMR spectra at a 20-fold ligand excess The concentrations of CtCBM11 and cellohexaose were 18 lM and 3 64 lM, respectively ... as lichenan, b-glucan, cellohexaose, cellotetraose, cellopentaose and G4G4G3G CtCBM11 exhibits a preference for b-1,3-1 ,4 glucans and a considerable affinity for b-1 ,4 linked glucose polymers No ... two six-stranded anti-parallel b-sheets that form a convex side (b-strands 1, 3, 4, 6, and 12) and a concave side (b-strands 2, 5, 7, 8, 10 and 11) The concave side is decorated by the side chains...

Ngày tải lên: 18/02/2014, 17:20

... )117.5 ⁄ 1 34. 7 )90 .4 ⁄ )7.5 )117.8 ⁄ 120.9 )117.5 ⁄ )3.3 u⁄w )88.1 ⁄ 137.6 )133.0 ⁄ 144 .5 )89.0 ⁄ 9.8 )163.7 ⁄ 145 .3 )1 24. 9 ⁄ 0 .48 u⁄w )99.8 ⁄ 125.7 )162.8 ⁄ 146 .9 )1 04. 4 ⁄ 0.72 ) 94. 5 ⁄ 100.67 ... w4 N Ser49 Ne Arg45 CO Lys46 N Ala48 OH Tyr55 N Ile59 N Gly58 CO Lys97 OH Tyr79 Oc1 Thr98 CO Phe102 CO Val80 3.2 2.8 2.7 3.3 3.8 2.9 3.3 2.7 2.8 2.8 3.0 2.8 w4 N Ser49 Ne Arg45 CO Lys46 N Ala48 ... structural and unfolding properties of replacing the axial methionine ligand with a lysine residue has been further assessed 244 8 DGunf 4. 5 ± 0.5 3.2 ± 0 .4 3.1 ± 0.3 3.0 ± 0 .4 m 2.3 1 .4 2.0 1 .4 ± ±...

Ngày tải lên: 07/03/2014, 17:20

... (1.55–1.50 A) 31.6 5.7 7.2 48 .8 37 816 0.191 0.216 0.0 14 1.61 61.220 128.086 P41212 1 .45 ˚ 96 550 (30 1 .45 A) 42 718 96.8 ˚ 98.5 (1.50–1 .45 A) 16.7 2.2 6.5 41 .0 40 8 34 0.182 0.207 0.0 14 1.61 15.7/2288 ... 1.69 1. 24 229.10 1 94. 49 9.55 12. 64 1 .48 77 .48 60.89 187 .49 S)1 and S)2 S)3 S)2 S)1 M) ± 0.05b (1.000c) ± 0.06 (0. 947 ) ± 0.07 (1.290) ± 30. 93 (0.007) ± 6 .42 (0.008) ± 0.36 (0.168) ± 0. 34 (0.127) ... pEQW + pEKW Uncomplexed 2.28 2.23 2.18 2 .42 2.26 2 .46 2 .30 2. 24 2.19 2.36 2 .30 2 .49 2.31 2.21 2.19 2.37 2.13 2 .46 2.27 2.28 2. 24 2.31 stacked with the imidazole ring of His 143 , similar to some...

Ngày tải lên: 08/03/2014, 23:20

... Leu1 24 Leu135 Glu137 Tyr139 Leu28 Phe42 Leu 44 Leu46 Leu58 Phe60 Met73 Ala75 Val86 Tyr88 Asn92 Phe 94 Ala107 Leu109 Leu120 Gly122 Tyr1 24 Ile15 Leu29 Phe31 Phe33 Ile47 Phe49 Val62 Gly 64 Thr 74 Phe76 ... a hinge region ( 144 – 149 ) and a a helical domain ( 130 143 ) (Fig 2) Two conserved cysteines (Cys68 and Cys160) form the conserved disulphide bridge between the C-terminal tail and the b barrel ... androstenol and androstenone The previously identified natural ligands androstenol and androstenone have been built with the program ACD/CHEMSKETCH [18] The parameter and topology files for CNS [ 14] were...

Ngày tải lên: 17/03/2014, 17:20

... predicted pKa values were 6.15 and 4. 27 for BSL2, 5.93 and 4. 20 for HEWL, and 4. 89 and 3. 84 for house fly lysozyme Although these values not agree completely with the experimental results [11], the ... 17.3 m (kJỈmol)1 M) 10.7 7.97 Thermal unfolding Tm (K) 333.8 326.6 40 6 .4 386 .4 DH (kJỈmol)1)a a BSL2 HEWL 4. 17 53 .4 12.8 4. 16 41 .9 10.1 The unfolding enthalpies at transition temperature Tm Discussion ... acetate (for pH and 5) and sodium phosphate (for pH and 7) buffer The ionic strength of each buffer was adjusted to 0.1 [40 ] Lysozyme solution and M lysodeikticus suspension were mixed, and the decrease...

Ngày tải lên: 23/03/2014, 04:21

... (47 64) 16 .4 (25.3) 20.6 (33.7) 21.2 99.0 (96.7) 17.0 (3.9) 3.2 (3.2) 5.8 ( 54. 2) C2221 74. 77 1, 1.0, 1.0 PROCHECK 91.1 8 .4 0.3 0.3 1.680 37.3 30. 2 33.9 36.1 0.018 [50] 179 28 944 (41 61) 17.3 ( 24. 7) ... 1.90 2.02 99 .4 (100.0) 15.2 (2.2) 4. 9 (4. 8) 5.7 (49 .7) 111.17 108.98 26.1 1.90 2.02 C2221 73.62 20.1 16.1 21.6 29.9 0.015 1.505 89 .4 10.3 0.3 308 33900 (48 12) 21 .4 (27.1) 25.0 (30. 0) 28.0 28.1 ... Mar˚ CCD 125 mm detector and radiation at 1 .43 1 A (PAH_1) All data sets were processed and scaled with the software mosflm [42 ] and scala [43 ] from the ccp4 suite [44 ] The structures were straightforwardly...

Ngày tải lên: 23/03/2014, 07:20

... fi Lys64a Tyr211 fi Ser13 Tyr211 fi Ser15 BCD-NDb ⁄ Boverall (%) 157.8 1 64. 7 127.3 139.3 149 .4 120.9 1 34. 5 142 .2 146 .8 139.9 153.8 144 .2 147 .9 132.9 14. 2 14. 4 8.5 9.8 12.1 8.2 11.2 12.5 8 .4 12.2 ... amides of Asn58 and Asn85 The C4-hydroxyl group of shikimate interacts with the carboxylate group of Asp100 and with the side-chain amides of Asn85 and Lys 64 The C3-hydroxyl group forms extensive hydrogen ... central six-strand mixed b sheet (b2, b1, b3, b5, b6 and b4; b5 is antiparallel to the others) flanked by three a-helices (a1, a9 and a8) on the inner side and by two a-helices (a2 and a3) and two...

Ngày tải lên: 30/03/2014, 02:20

Ngày tải lên: 03/05/2014, 17:07

... u| p (x) ∂p ∂xj − p (x) p2 (x) | u| p (x) ln | u| we have | u| p (x) + n j=1 Ω∩BR xj ∂ p (x) ∂xj n − | u| | u| p (x) n − j=1 (x, p (x) ) p2 (x) p (x) | u| ln | u| p (x) dx p (x) −2 ∂u ∂u ∂xi ∂xj xj νi ds ∂(Ω∩BR ... p (x) −2 ∂u ∂ u xj ∂xi ∂xi ∂xj dx i,j=1 Ω∩BR n dx p (x) −2 ∂u ∂u u| ∂xi ∂xj xj νi ds | i,j=1 ∂(Ω∩BR ) Introducing the following result | u| p (x) −2 n i=1 ∂u ∂ u ∂xi ∂xi ∂xj ∂ p (x) ∂xj = | u| p (x) ... (u) xj νj ds p (x) | u| i,j=1 Ω∩∂BR p (x) p (x) −2 ∂u ∂u ∂xi ∂xj xj νi | u| n + − a | u| | u| i,j=1 ∂Ω∩BR n p (x) p (x) | u| − H (x) F (u) xj νj ds p (x) −2 ∂u ∂u ∂xi ∂xj xj νi p (x) − H (x) F (u) xj νj...

Ngày tải lên: 20/06/2014, 21:20

... f dx d y dx d y 1/2 1/2 dx d y (y)g (y)d y 1/2 ∞ f (x) dx 1 /4 1/2 ∞ (x) f (x) dx y x 1/2 g (y) y A {x, y } + B max {x, y } x ∞ g(y) 1/2 f (x) x A {x, y } + B max {x, y } y ∞ 1 /4 x f (x) A {x, y ... (x) g(y) dx d y Amin {x, y } + B max {x, y } ∞ ∞ 0 f (x) dx g(y)d y Amin {x, y } + B max {x, y } ∞ ∞ 0 f (x) dx Amin {x, y } + B max {x, y } (2.20) 1/2 dy By (2.17), we have (2.6) Thus (2.6) and ... = ( f (x) /(Amin {x, y } + B max {x, y }))dx, then by (2.6), we get ∞ 0< = = g (y)d y ∞ ∞ 0 ∞ ∞ 0 f (x) dx Amin {x, y } + B max {x, y } dy (2.18) f (x) g(y) dx d y Amin {x, y } + B max {x, y }...

Ngày tải lên: 22/06/2014, 06:20

... in Σ = P G(3, q) and let E = Σ \ Π The code C can be extended if and only if there exists a partition {X1 , X2 , , Xq } of E where each Xi is a transversal set of T Geometry and Combinatorics ... Forum Geom., 2:135– 146 (electronic), 2002 [8] L Lov´sz and A Schrijver Remarks on a theorem of R´dei Studia Sci Math a e Hungar., 16(3 -4) :44 9 45 4, 1983 [9] Jacobus H van Lint and Gerard van der ... codes and Bruen-Silverman codes PhD Thesis, University of Western Ontario, 2002 [2] T L Alderson and A A Bruen Coprimitive sets and inextendable codes Des Codes Cryptogr., 47 (1-3):113–1 24, 2008...

Ngày tải lên: 07/08/2014, 15:23

... 285 299 307 319 317 316 328 340 361 397 43 3 45 5 49 8 5 54 642 713 765 8 64 867 47 1 2.9 2.8 2.8 2.9 2.9 3.0 3.0 3.0 3.1 3.1 3.2 3.5 3.7 4. 2 4. 2 4. 9 5.3 5.7 6 .4 5.8 3.8 47 .4 48.2 44 .4 40.6 41 .2 40 .1 ... 0.3 74 1058 17.9 0. 346 1 144 0 .49 8 795 0 .49 3 8 04 12 42 5 4. 6 35.1 28 .4 0 .41 2 1032 16.9 0.3 84 1106 0.523 813 0.518 821 13 47 9 5.6 35.2 28.3 0 .44 3 1082 17.0 0 .41 3 1159 0. 549 873 0. 542 8 84 14 530 6.1 ... 1 043 10 42 0 3 .4 36.1 30. 7 0.366 1 147 17.9 0. 342 1227 0 .43 1 9 74 0 .40 1 1 048 11 45 3 3.6 34. 8 30. 7 0.395 1 148 17.6 0.369 1226 0 .46 5 9 74 0 .43 5 1 041 12 505 3.9 31.5 30. 6 0 .43 6 1157 16.7 0 .41 0 1232...

Ngày tải lên: 08/08/2014, 01:22

... Modulation Transfer Function 1.0 0.8 X- ray magnification x3 x1 0.6 0 .4 10 % limit 0.2 0 10 12 14 16 18 20 Figure Evaluation of the modulation transfert function using an X- ray image of the bar/space ... International 34 (2001) 345 –353 [5] Bergander A., Salmen L., Variations in transverse fibre wall properties: relations between elastic properties and structure, Holzforschung 54 (2000) 6 54 660 [6] ... measurements of linear X- ray detectors, J X- ray Sci Technol (1996) 205–221 [ 14] Kaftandjian V., Zhu Y.M., Peix G., Babot D., Contrast transfer function measurement of X- ray solid state linear...

Ngày tải lên: 08/08/2014, 14:21

... London, UK)) and random hexamers (Roche, East Sussex, UK)) RNA was extracted and cDNA synthesized from 64 lumbar IVD samples (NP and AF samples) for gene expression analysis (consisting of 24 non-degenerate ... IVD L4/5 34 Surgical F 25 Herniated IVD L5/S1 35 Surgical M 35 Herniated IVD L4/5 36 Surgical M 44 Herniated IVD L5/S1 37 Surgical M 64 Herniated IVD L5/S1 38 Surgical M 28 Herniated IVD L4/5 ... applicable L4/5 Not applicable L5/S1 Relatively normal L4/5 47 Relatively normal L4/5 M 75 Not applicable L5/S1 M 35 Mild degeneration L5/S1 Surgical M 48 Mild degeneration L3 /4 Surgical F 64 Mild...

Ngày tải lên: 09/08/2014, 10:20

... of (page number not for citation purposes) Journal of Inflammation 2008, 5:2 38 39 40 41 42 43 44 45 46 47 48 49 50 http://www.journal-inflammation.com/content/5/1/2 Barrat FJ, Cua DJ, Boonstra ... CD4+CD25+ and CD4+CD45RBlow cells function via the action of IL-10 [26], cytotoxic T-cell-associated protein (CTLA4) [27,28], and/ or TGF-β [26-33], while other Tregs secrete IL-10 [31 -40 ] and ... perforin-expressing CD8+ T-cells, which otherwise, could mediate inflammation [42 ,44 ,45 ] Both Th1 and Th2 responses can be controlled by IL-10 Tregs and by naturally occurring Tregs [46 ] It is...

Ngày tải lên: 11/08/2014, 08:21

... dexamethasone therapy [4] References Conclusion We described a 3-year-old boy with X- ALD, adrenal insufficiency and CPAM The early and accurate diagnosis and treatment of patients with X- ALD and ... morbidity and death in childhood Pediatr 2007, 119:e4 84- e4 94 Azizkhan RG, Crombleholm TM: Congenital cystic lung disease: contemporary antenatal and postnatal management Pediatr Surg Int 2008, 24: 643 -657 ... patient and, most likely his deceased uncle and four brothers had X- ALD This disorder was probably the cause of their various constitutional symptoms and the unexpected death of his male relatives X- ALD...

Ngày tải lên: 11/08/2014, 14:21

... prevention and policy 2009, 16:7-38 Pre-publication history The pre-publication history for this paper can be accessed here: http://www.biomedcentral.com/ 147 1- 24 4X/ 11/81/prepub doi:10.1186/ 147 1- 24 4X- 11-81 ... urine samples at admission Amphetamine 17 Benzodiazepines 60 14% 49 % Buprenorphine 56 46 % Cannabis 43 35% Cocaine 1% Dextropropoxyphene 4% Methadone 7% Opiates 62 50% Discussion The program had a ... Sublingual-Tablet Formulation of Buprenorphine and Naloxone New England Journal of Medicine 2003, 349 : 949 -58 36 Kakko J, Dybrandt Svanberg K, Kreek MJ, Helig M: 1-Year retention and social function after buprenorphine-assisted...

Ngày tải lên: 11/08/2014, 15:22

... Results Time to Exhaustion The time to exhaustion of the EX-O group was 515 ± 30 minutes and 42 5 ± 30 for the EX group (p = 0.0 34) This represented a 20% higher exhaustion time for the EX-O group when ... NUTRIENT CONTROL % EXPERIMENTAL % Protein (g) 18 24. 8 17 .4 23.5 Fat(g) 12 .4 4.9 14. 8 Carbohydrate(g) 45 .5 62.7 45 .6 61.6 Total fibers (g) 21.9 - 18.9 - Insoluble fibers (g) 18 82 14. 4 76.1 Soluble ... stress and exercise and changes in peripheral leukocyte adhesion molecule expression and density Psychmestry Medicine 2000, 62:6 64- 670 42 Gleeson M, Nieman D, Pedersen BK: Exercise, nutrition and...

Ngày tải lên: 11/08/2014, 23:21