... triplicate for each CaN heterodimer CaNa, d; CaNb, s CaM dissociation constants for both CaNa and CaNb Fast and slow rates of s)1 and 0.4 s)1, and 3.9 s)1 and 0.4 s)1 were obtained for CaNa and CaNb, ... composed of CnAa or CnAb catalytic subunit isoforms by CaP (A) The reactions proceeded for 10 at 30 °C using 32 lM and 91 lM PO4-RII peptide for CaNa or CaNb, respectively CaNa or CaNb were each ... CaN heterodimers containing the CnAa or CnAb catalytic subunit and pNPP as substrate, we measured IC50 values of 20 lM for CaNa and 90 lM for CaNb Furthermore, in contrast to the results obtained...
Ngày tải lên: 24/03/2014, 03:21
... authors and readers, this journal provides supporting information supplied by the authors Such materials are peerreviewed and may be re-organized for online delivery, but are not copy-edited or ... glutamic acid and histidine as important catalytic residues, as suggested by Takahashi and More [5], also applies to RNase Sa2 Moreover, the structures provide more detailed information about ... Phe39 and interacted with Arg42 Guanine forms the most efficient hydrogen-bonding network with the enzyme, and this seems to be very important for proper enzyme–base binding Other bases form a...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx
... at °C for 24 h (right) Lanes b, the nicked precursor was incubated at pH 7.0 and °C for indicated periods of time Lane c, the nicked precursor was incubated at pH 7.0 and °C for 24 h and then ... the nicked and intact forms of the precursor) Proteins were detected by silver staining The band of the 38-kDa mature form is smeared in nondenaturing PAGE (lower panel, lanes a and c) For details ... hydrogen-bond donor and Ser278 is an acceptor In the structures of the wild-type kumamolisin-As and two of its mutants, the conformation of the catalytic Ser278 is quite similar, with the side chain torsion...
Ngày tải lên: 19/02/2014, 07:20
Báo cáo khoa học: Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution ppt
... Korea), and the DNA sequencing was performed by Solgent Co (Daejeon, South Korea) K10 E15 P16 N–TER K12 V14 P16 V14 A13 A13 K12 E15 K10 [411–420] Hydrophobic Core [57–69] Random mutagenesis and ... PM0075863 and PM0075934, respectively When the open conformation was superimposed on the open and closed templates (1C7G and 2VLH, respectively), the rmsd values were 0.37 ˚ and 1.97 A for the Ca ... the structural rationale for the other stability (E83K and T407A) and activity (T129I and T451A) mutations was investigated on the basis of the open and closed conformation models As a result,...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Respective roles of the catalytic domains and C-terminal tail peptides in the oligomerization and secretory trafficking of human acetylcholinesterase and butyrylcholinesterase potx
... acetylthiocholine or butyrylthiocholine for BChE We thank Dr Oksana Lockridge for the generous gift of vectors expressing wild-type and mutated human AChE and BChE, and Dr Yaacov Ashani for DEPQ This work ... during brain development [40,48,49] Both catalytic and noncatalytic functions clearly require appropriate oligomeric organization and localization and therefore depend on the C-terminal t peptides, ... obtained for Aa and Ba were almost the same, except for a shift in the sedimentation coefficients, which are higher for BChE than for AChE By contrast to Aa and Ab, oligomers of Ba and Bb represented...
Ngày tải lên: 07/03/2014, 03:20
314 CMR 9.00: 401 WATER QUALITY CERTIFICATION FOR DISCHARGE OF DREDGED OR FILL MATERIAL, DREDGING, AND DREDGED MATERIAL DISPOSAL IN WATERS OF THE UNITED STATES WITHIN THE COMMONWEALTH potx
... melt, and stormwater runoff; and Footpaths, bikepaths and other paths for pedestrian and /or nonmotorized access (d) For phased projects the determination of whether the Stormwater Management Standards ... government, public or private corporation or authority, individual, partnership or association, or other entity, including any officer of a public or private agency or organization Qualified Environmental ... wetlands, and other waters or wetlands within the borders of the Commonwealth where the use, degradation, or destruction could affect interstate or foreign commerce as determined by the Corps...
Ngày tải lên: 15/03/2014, 16:20
Báo cáo khoa học: Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production, catalytic properties and structure pdf
... authors and readers, this journal provides supporting information supplied by the authors Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or ... The authors wish to thank H Boer for help with CD, P Matikainen for help with the MALDI-TOF analysis, O Liehunen and B Hillebrandt-Chellaoui for excellent technical assistance, and B Smit for fermentor ... lanes and 5, and the less overglycosylated laccase ScMaL pool II in lanes and siae and T reesei Also, the temperature stability was similar for ScMaL and rMaL The N-terminal sequencing verified correct...
Ngày tải lên: 16/03/2014, 00:20
synthesis of pd or pt titanate nanotube and its application
... al / Sensors and Actuators B 128 (2007) 320–325 321 nanotubes by a hydrothermal method and prepared a flat-type catalytic combustion hydrogen sensor on an alumina substrate using both Pd and Pt/titanate ... 48 and 62 can readily be indexed to lepidocrocite-type titanate phase (e.g., orthorhombic H2 Ti2−x/4 x/4 O4 , a = 0.3643, b = 1.8735 and C = 0.2978 nm) and correspond well with 0, 1 0, 0, 0 and ... to catalytic poisoning and reduce the sensor’s maximum response temperature to 120 ◦ C [4] We have also reported reduced maximum temperature of a flat-type catalytic hydrogen sensor using TiO2 and...
Ngày tải lên: 20/03/2014, 13:08
Báo cáo khoa học: Multidrug efflux pumps: drug binding – gates or cavity? Emily Crowley and Richard Callaghan pdf
... supporting data for both models More correctly, it is interpretation of the supporting data that requires further consideration or refinement This section highlights many of the drawbacks or limitations ... Authors Journal compilation ª 2009 FEBS 533 Drug binding – gates or cavity? E Crowley and R Callaghan TM8 and TM11 accounted for > 70% of the total bound drug Based on homology modelling and ... helices (TM1, TM4-7 and TM9-12) may line the central cavity, contribute to the drug-binding pocket and ⁄ or regulate conformational coupling within ABCB1 It is worth noting that TM6 and TM12 have consistently...
Ngày tải lên: 22/03/2014, 21:20
Báo cáo khoa học: Directed evolution of a histone acetyltransferase – enhancing thermostability, whilst maintaining catalytic activity and substrate specificity doc
... Investigator We thank the BBSRC for funding, Dr Sophie Jackson for critical reading the manuscript, Dr J M Denu for the pRSET-P ⁄ CAF vector, Mike Weldon for peptide synthesis, Dr Chris Johnson for ... uses random mutagenesis and subsequent screening to identify substantially more thermostable mutants of the catalytic domain of the human HAT P ⁄ CAF, without affecting its catalytic activity or ... assay and synthetic peptide substrates Wild-type P ⁄ CAF has kcat and KM values of 12 min)1 and 53 lm with the H3 peptide and 0.29 min)1 and 194 lm with the H4 peptide, whereas the KM for acetyl-CoA...
Ngày tải lên: 23/03/2014, 06:20
Báo cáo khoa học: Purification of three aminotransferases from Hydrogenobacter thermophilusTK-6 – novel types of alanine or glycine aminotransferase Enzymes and catalysis pot
... mm Glu and 20 mm glyoxylate for GGT, 20 mm Glu and 30 mm pyruvate for GPT, 40 mm Ala and mm glyoxylate for AGT, and 10 mm phosphoserine and 10 mm 2-OG for PSOT For the AT1 assay, the pH in the ... authors and readers, this journal provides supporting information supplied by the authors Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or ... and 150 mm NaCl at flow rate of 0.5 or mLÆmin)1, respectively Gel Filtration Standard (Bio-Rad) was used as a molecular maker for calibration Each measurement of standards or samples was performed...
Ngày tải lên: 29/03/2014, 09:20
Statements of Financial Position As of June 30 or December 31, 2009 and 2008_part2 ppt
... rates for out-of-state students Grants and contracts from state, local and private funding sources, and facilities and administrative cost allowances, increased by about $875 thousand Funding for ... revenues and expenses for the years ended June 30, 2009, 2008, and 2007: For the years ended June 30, (stated in millions) REVENUES 2009 Amount Percent Tuition and fees, net Federal grants and contracts ... salaries and benefits, other post employment benefits, and utility and supply costs, of $11.6 million, $7.4 million, and $4.4 million, respectively Salary increases in FY08 for classified staff and...
Ngày tải lên: 18/06/2014, 20:20
Statements of Financial Position As of June 30 or December 31, 2009 and 2008_part4 potx
... University System, for the purpose of financing or refinancing the acquisition and installation of equipment or personal and real property and infrastructure improvements The Montana Science and Technology ... used for the purpose of restructuring Series B, C and D Facilities Improvement Revenue Bonds, and for the acquisition, construction, remodeling, improvement and equipping certain facilities and ... tuition and fees Auxiliary enterprises and other operating activities Private grants and contracts Other Gross accounts and grants receivable Less: allowance for doubtful accounts Net accounts and...
Ngày tải lên: 18/06/2014, 20:20
Statements of Financial Position As of June 30 or December 31, 2009 and 2008_part5 pot
... law and include biweekly payments for temporary loss of wages as well as qualifying permanent partial and permanent total disability Medical and indemnity benefits are statutorily prescribed for ... accordance with state law Annual reports that include financial statements and required supplemental information on the plan are available from: TIAA-CREF 730 Third Avenue New York, New York ... and the historical pattern of sharing of benefit costs between the employer and plan members to that point The projection of benefits for financial reporting purposes does not explicitly incorporate...
Ngày tải lên: 18/06/2014, 20:20
báo cáo hóa học: " Virtual reality and physical rehabilitation: a new toy or a new research and rehabilitation tool?" pptx
... presence and its importance to the use of VR for rehabilitation Why use a virtual world for rehabilitation? References Many people question why we don't just have subjects perform motor tasks ... abilities, a safe testing and training environment, the opportunity for graduated exposure to stimuli, the ability to distract or augment the performer's attention, and perhaps most important to therapeutic ... strategies of reach, grasp, and place movements performed with physical and virtual objects by healthy adults and those with hemiparesis Sveistrup presents current work on motor rehabilitation using...
Ngày tải lên: 19/06/2014, 10:20
Báo cáo y học: "Subcutaneous Immunoglobulin-G Replacement Therapy with Preparations Currently Available in the United States for Intravenous or Intramuscular Use: Reasons and Regimens" docx
... inflammatory reactions and /or the release of mediators from mast cells.4,6,19,20 Subsequently, several groups showed that these infusions can be given much more rapidly and have reported rates ... previous and current treatment regimens (if available) and details of the current subcutaneous regimen were recorded All patients gave informed consent for review of their medical records This ... reason for interest in subcutaneous infusions was adverse effects of IV infusions These included chills and rigours during IGIV infusions and /or severe headaches, often with nausea and vomiting or...
Ngày tải lên: 08/08/2014, 20:23
Báo cáo y học: "Analysis of bronchoalveolar lavage fluid proteome from systemic sclerosis patients with or without functional, clinical and radiological signs of lung fibrosis" pot
... [21,22] Furthermore, an alteration in the production of proinflammatory and /or pro-fibrotic cytokines and chemokines, growth factors (in particular transforming growth factor-β), and signaling ... data analysis and performed statistical analysis CL and CV selected patients and revised all clinical charts PE was involved in the coordination of the study and in the discussion and revision ... method) and cultures for mycobacteria, and for the microscopic examination of Pneumocystis jiroveci (Gomori-Grocott silver stain) All the other aliquots were used to assess the total and differential...
Ngày tải lên: 09/08/2014, 08:22