opg anti il 17 antibody and etanercept

... antibody was incubated overnight at room temperature As a secondary antibody, biotinylated goat anti- rabbit antibody was used and detected with biotin-streptavidin-peroxidase staining (Elite kit; ... 35SO42- incorporation into patellar and tibial cartilage was assessed The cartilage of the patella and the tibia was isolated 3, 7, and 21 days after viral injection and incubated with 35SO42- for ... cartilage and osteophyte Osteoarthritis Cartilage 2003, 11:278-284 31 Fukui N, Zhu Y, Maloney WJ, Clohisy J, Sandell LJ: Stimulation of BMP-2 expression by pro-inflammatory cytokines IL- 1 and...

Ngày tải lên: 09/08/2014, 10:21

... AJ, Soliman H, Kaiser W, Miller LK Anti- and proapoptotic activities of baculovirus and Drosophila IAPs in an insect cell line Cell Death Differ 1997, 4: 733–44 13 Hildt E, Munz B, Saher G, Reifenberg ... Therefore, the band with molecular weight around 4.5 kilo base pairs detected by cIAP2 probe was expected as the cIAP1 RNA (Figure 1B, the band below cIAP2 band) The nature of the third band below ... IAP-family genes in human cancers and myeloid leukemias Clin Cancer Res 2000, 6 :179 6–803 41 Wang CY, Mayo MW, Korneluk RG, Goeddel DV, Baldwin AS Jr NF-kappaB antiapoptosis: induction of TRAF1 and...

Ngày tải lên: 02/11/2012, 11:17

... RAB25 and S100P antibodies The following antibodies, dilutions, and pretreatment conditions were used: antiRAB25 (1:100), trypsin pretreatment; Abnova Corporation, Taipei, Taiwan) and anti- S100P ... for each gene and used as the standard sample in the subsequent assay Each standard curve consisted of six serial dilutions (1, 1/5, 1/25, 1/125, 1/625, and 1/3125) of the selected standard cDNA ... Figure Quantification and validation of 19 genes by qPCR The expression of 19 genes in test lung cancer cell lines (black and gray) and four validation cell lines (white) was quantified by qPCR and...

Ngày tải lên: 15/02/2014, 04:20

... Relative IL6 R mRNA level A L.-H Zhu et al 0.6 0.4 0.2 pSilencer/ pSilencer/ NC sh -IL6 R pSilencer/ pSilencer/ NC sh -IL6 R * 90 80 70 60 50 40 30 20 10 pSilencer/NC pSilencer/sh -IL6 R Fig Knockdown of IL6 R ... pSilencer/ sh -IL6 R 1.2 * 0.40 0.35 0.30 0.25 0.20 0.15 0.10 0.05 1.0 0.6 0.4 0.2 24 h 48 h pSilencer/ pSilencer/ NC sh -IL6 R C 0.8 pSilencer/NC pSilencer/sh -IL6 R Number of colonies Relative IL6 R ... enhanced chemiluminescence and exposure to chemiluminescent film LabWorksÔ Image Acquisition and Analysis Software was used to quantify the band intensities The antibody to IL6 R was purchased from...

Ngày tải lên: 18/02/2014, 04:20

... fertile and survive for at least year under basal conditions [28], but show ejaculatory abnormalities [29] and high susceptibility to oxygen toxicity [30] Recent studies of our group [31] and ... synthesis and heme degradation is responsible To explore the role of free heme in HO-1 and HO-2 expression, we treated HeLa and HepG2 cells with SA and determined the expression levels of HO-1 and ... (C and D) were left untreated or treated with SA (5 mM) for the indicated time and harvested The upper panels in (A) and (C) show the northern blot analysis of HO-1 and HO-2 mRNA in HeLa (A) and...

Ngày tải lên: 19/02/2014, 05:20

... antibody Note that HO-1 mRNA and protein were undetectable in Jurkat and K562 cells under the conditions used bone marrow origin, including KG1 myeloid cells, Jurkat T-lymphocytes, and K562 and ... (Immobilon-P, Millipore Corporation, Billerica, MA, USA) The membranes were treated for h at room temperature (20–22 °C) with anti- HO-1 (a gift from Shigeru Taketani) [40] or anti- HO-2 antibody (Stressgene ... mobilization and utilization of cellular iron and heme: endless findings and growing evidence of tight regulation Tohoku J Exp Med 205, 297–318 3146 32 Fujita H & Sassa S (1989) The rapid and...

Ngày tải lên: 19/02/2014, 06:20

... Ber-T9 monoclonal antibodies (anti- CD71) and rabbit anti- ferritin Igs, followed by FITC-conjugated rabbit antimouse and FITC-conjugated swine anti- rabbit Igs, respectively Mouse and rabbit Igs were ... The authors would like to thank L Mayer and K Uchida for providing antibodies, A Vale, F Pisarra and M.T Silva for help and comments on TEM, and M Santos and R Hultcrantz for critical reading of ... 43, a monoclonal antibody to human MHC class II molecules (DAKO); Nor3.2 a monoclonal antibody to non-native human CD1d (BIODESIGN, Saco, ME, USA [26]); CD1d42, a monoclonal antibody to native...

Ngày tải lên: 19/02/2014, 16:20

... nuclear-specific antibodies anti- TBP or anti- Egr1, as described in the Materials and methods As a loading control, the presence of b-actin protein was also confirmed by using specific antibody, as ... proteins and lg of polyclonal anti- eEF1A (lane 4), with nuclear proteins and 0.9 lg of polyclonal anti- eEF1A (lane 5), with nuclear proteins and lg of total CRS (lane 6), with nuclear proteins and ... water and incubated for 1.5 h with an anti- mouse IgGconjugated horseradish peroxidase secondary antibody (Promega, Madison, WI, USA) After washing once with NaCl/Pi containing 0.05% Tween-20 and...

Ngày tải lên: 21/02/2014, 00:20

... obtained after 2–3 weeks and re-cloned by serial dilution The stable cells transfected with sense and antisense PKB were named S-PKB/H7721 and AS-PKB/H7721 Cell culture and treatment Cells were ... subjected to Western blotting and detected by using phospho-GSK-3a/b (Ser21/9) antibody and ECL reagents Finally, the intensity of the GSK bands on X-ray film was quantified by densitometric scanning ... migration and invasion with monoclonal antibody For inhibition of cell adhesion, migration and invasion, H7721 cells were preincubated with 10 ngÆmL)1 or 20 ngÆmL)1 different mAbs against Lewis antigens...

Ngày tải lên: 21/02/2014, 00:20

... dilution), and with polyclonal antibodies (1 : 1000 dilution) specific for (D) NF-jB1 (p50 and p105) and (E) RelA (p65) Total cell lysate (C) was incubated with antibodies to total ERK1 ⁄ and p38, ... Primary antibodies were incubated at °C overnight, and horseradish peroxidase (HRP)-conjugated secondary antibody (1 : 20 000 dilution) was incubated for 1.5 h at room temperature The antibody ... PAGE and transferred to nitrocellulose Blots from cytosolic and nuclear fractions were incubated with antibodies to (A) phospho-ERK1 ⁄ (1 : 1000 dilution) and (B) phospho-p38 MAPK (1 : 1000 dilution),...

Ngày tải lên: 07/03/2014, 12:20

... Primary antibody probing was performed with anti- p21waf1 (0.2 lgÆmL)1), anticyclin D (2 lgÆmL)1), anti- CDK Ó FEBS 2004 CRE-decoys in colorectal cancer cells (Eur J Biochem 271) 2775 (2 lgÆmL)1), anticyclin ... di Napoli, Italy), and were maintained in McCoy’s 5A with 10% (v/v) fetal bovine serum HCT116 and GEO cell lines were both wild-type p53, and SW620 lines were mutant p53 No antibiotics were used ... 5-fluorouracil (5-FU) or SN38 on cell growth and viability; and third, to elucidate the cellular mechanisms underlying any synergistic effects seen in the drug combinations Materials and methods...

Ngày tải lên: 07/03/2014, 15:20

... buffer and boiling Antibodies were used at the following dilutions: anti- hCdt1, : 500; anti( h-geminin) (Santa Cruz), : 500; anti- Gem2, : 2000, anti- hCdc6/18 (Upstate Biotechnology), : 1000; anti- cyclin ... serum in NaCl/Pi for h and incubated overnight at °C with anti- hCdt1 or antigeminin Ig (Santa Cruz) at : 100 dilution in blocking buffer Secondary antibodies, anti- rabbit or anti- goat horseradish ... full-length Cdt1 and geminin (HisT7-geminin and His-geminin, amount of recombinant protein run in each lane in ng is indicated) and immunoblotted with anti- Cdt1 and anti- geminin Ig See Materials and methods...

Ngày tải lên: 07/03/2014, 16:20

... Western blot analysis using anti- TPH serum as the primary antibody and by (32P) autoradiography using a FLA-3000 fluorescence image analyzer (details are described in Materials and methods) Size markers ... visualized using an enhanced chemiluminescence detection system (ECL; Amersham, Buckinghamshire, England) Protein bands were exposed to an X-ray film (Konica, Medical Film 20287) For autoradiography ... presence and absence of okadaic acid and/ or MG132 By Western blot analysis of the whole cell extracts, TPH of molecular mass 53 kDa was localized side-by-side with purified TPH and the anti- TPH...

Ngày tải lên: 08/03/2014, 16:20

... weighing between 300 and 340 g were individually housed and maintained on a 12 light: 12 h dark cycle beginning at 06.00 hours Tap water and standard laboratory chow were available throughout After ... of h-orexin A and B, C-terminal orexin A and B fragments and reported antagonists on SK-N-MC cells and CHO cells stably transfected with the human OX1 receptor (100 pM radioligand) Ox1 receptor ... ethanedithiol (15.7 lL, 0.2 molÆL)1) and trimethylsilylbromide (13 lL, 0.1 molÆL)1) [24] The solution was shaken for 40 at room temperature and the peptide was precipitated and washed as described Purification...

Ngày tải lên: 08/03/2014, 22:20

... (both internalized and endogenous cystatin C were visualized) The primary antibody was polyclonal rabbit anti- (human cystatin C) serum [20] and the secondary antibody used was an anti- (rabbit-IgG) ... a primary antibody against cystatin C and a secondary antibody conjugated with Alexa-568 fluorophore (Fig S2) There was no fluorescence labelling in control cells or in cells used in antibody specificity ... goat and conjugated with Alexa-568 Further control experiments included primary antibody omission and antigen absorption FEBS Journal 275 (2008) 4571–4582 ª 2008 The Authors Journal compilation...

Ngày tải lên: 16/03/2014, 06:20

... western blot with the antieEF1A antibody of the protein recovered from the gel band is shown To completely elucidate the relationship between the structure of the GTs and their ability to inhibit ... demonstrating a full G-based structure, showed a fast and a slow migrating band similarly to GT-G4 (data not shown) Figure 6B illustrates the stability of GRO26A and GT-G4: these structures were not disrupted ... used two oligomers, GRO29A and GRO26A, whose structures were related to antiproliferative activity in tumour cells [10] As illustrated in Fig 6A, the weak CD bands of GT and GT-G1 indicate absence...

Ngày tải lên: 16/03/2014, 13:20

... (A, B, C and D) and glycoproteins (E, F, G and H) were analyzed by HPLC as described in Experimental procedures (D) and (H) correspond to the pattern of oligomannosides bound to LLO and glycoproteins, ... residue and nine Man and two GlcNAc residues at the reducing end M4Gn1, M5Gn1, M6Gn1, M7Gn1, M8Gn1, and M9Gn1 indicate oligomannosides with four, five, six, seven, eight and nine Man residues and ... oligomannosides containing one Glc, nine Man, and two GlcNAc residues M3, M4, M5, M8 and M9 indicate oligomannosides containing three, four, five, eight and nine Man residues and two GlcNAc residues, respectively...

Ngày tải lên: 16/03/2014, 16:20

... Table 1) and the highest GPx activity (Fig 2), whereas bone- and muscle-derived q0 cells have rather similar GPx activities and similar capacities to eliminate intracellular oxidants under standard ... mM)1Æcm)1 [39] Reverse transcription and quantitative PCR RNA (5 lg) was reverse transcribed to cDNA using random hexamer primers and the Stratascript enzyme Quantitative PCR was performed using ... primary antibodies to CuZnSOD (Santa Crutz) or MnSOD (Stressgen Biotechnology), membranes were probed with horseradish peroxidase-conjugated secondary antibody (Amersham Biosciences) Bound antibody...

Ngày tải lên: 16/03/2014, 18:20

... without (lane 2) IL- 2 were developed by anti- CD25 Ig (antiTac Ig) (lane 1,2), MIKb1 [anti- (IL- 2Rb) Ig] (lane3), TUGH4 [anti( IL- 2Rc) Ig] (lane 4), anti- CD71 Ig (MEM-75) (lane 5) and anti- CD48 Ig (MEM-102) ... following antibodies: IL- 2Ra was targeted by anti- Tac Ig (IgG2a), while monoclonal anti( Mik-b3) Ig (IgG1j) and anti- TUGh4 Ig (Pharmingen, San Diego, CA, USA) were used against the IL- 2Rb and cc ... J., Alileche, A., Goldman, C.K., Sharrow, S & Waldmann, T.A (1997) Preassembly of interleukin (IL- 2) receptor subunits on resting Kit 225, K6 T cells and their modulation by IL- 2, IL- 7, and IL- 15:...

Ngày tải lên: 17/03/2014, 23:20

... are illustrated using a box plot The line inside each box is the median; the upper and lower limits of the box are the 75th and 25th percentiles, respectively, and the vertical bars above and ... a fragile cancer-relevant genomic region in chromosome 17 (17p13.3), and mapped to an exon of the C17orf91 gene [4,30] To date, several genes, including HOXA6, HOXA4, HSPG2, GPNMB, CLIC4 and SP1, ... MCF-7 and MDA-MB-231, P = 0.0012 for the comparison between T-47D and MDA-MB-231 and P = 0.0010 for the comparison between BT-474 and MDA-MB231, but P = 0.0399 for the comparison between MCF-7 and...

Ngày tải lên: 22/03/2014, 21:20