of recombinant adeno associated virus vectors

... values are expressed as means ± SD Results Distribution of recombinant adeno-associated virus vectors in mouse tissue The distribution of the rAAV-hAGA vector was assessed by isolating genomic ... limitations of a short half-life, requirement for frequent administration, and limited efficacy for patients with renal failure Therefore, we investigated the efficacy of recombinant adeno-associated virus ... TA, Kay MA: Recruitment of single-stranded recombinant adeno-associated virus vector genomes and intermolecular recombination are responsible for stable transduction of liver in vivo J Virol...

Ngày tải lên: 10/08/2014, 05:21

... by recombinant adeno-associated virus vectors J Virol 1996, 70:3227-3234. 28. Xiao W, Warrington KH Jr, Hearing P, Hughes J, Muzyczka N: Ade-novirus-facilitated nuclear translocation of adeno-associated ... of serotype 1, 2, and 5 recombinant adeno-associated viral vectors Methods 2002, 28:158-167. 32. Schmidt M, Katano H, Bossis I, Chiorini JA: Cloning and character-ization of a bovine adeno-associated ... organization of the adeno-associated virus 2 genome J Virol 1983, 45:555-564. 11. Owens RA: Latent infection of the host cell by AAV and its dis-ruption by helper viruses In Parvoviruses Edited...

Ngày tải lên: 12/08/2014, 04:21

... recom-binant adenoassociated virus (rAAV) to deliver and express normal, non-mutated RPE65 cDNA [16,22,23] The use of rAAV-mediated gene therapy has attracted much interest as it demonstrated a number of ... × 1010 particles of rAAV.RPE65 or rAAV.RPE65.1 was then deliv-ered into the subretinal space of the mouse eye, the diam-eter of which is about 3.5 mm Successful delivery of virus into the subretinal ... rAAV.RPE65.1 Analysis of RPE/ choroid and retina flatmounts of rAAV.RPE65-injected Rpe65-/- mice showed that the area of RPE65 expression covered approximately 30% of the surface of the RPE/ choroid...

Ngày tải lên: 14/08/2014, 19:22

... the E2A and E4 sequences from the adenovirus to produce the recombinant AAV2 (rAAV2) One of the advantages of using pDG is that no replication-competent adenovirus has been detected To produce ... adeno-associated virus, rAAV2-TTR-RB15 or Trang 4rAAV2-TTR-RB15 mutant (as control) of 2 × 1011 virusparticles was injected into the jugular vein of 2-months-old mice (n = 10 for each rAAV2 virus ... as % of cells treated with inactive RB15-mutant The results are shown as means ± stand-ard deviation of triple experiments Experiment of cells infected with adenovirus-RB15-mutant, adenovirus-RB15...

Ngày tải lên: 14/08/2014, 19:22

... gene transfer for MPS IVA with the use of adeno-associated virus (AAV)-based vectors, we have compared the expression level of GALNS under the control of either the CMV immedi-ate early enhancer⁄ ... potential application of AAV vectors for the treatment of Morquio A dis-ease, depending on the combined choice of transduced cell type, selection of promoter, and cotransduction of SUMF1 Abbreviations ... long-term in vivo studies: (a) evaluation of silencing of the promoter, and the resultant level of coexpression of SUMF1 and GALNS; and (b) confirmation of tar-geting of the expressed enzyme into affected...

Ngày tải lên: 29/03/2014, 21:20

... Samulski RJ: Adeno-associated viral vectors as gene delivery vehicles Int J Mol Med 2000, 6:17-27. 12 Carter BJ: Adeno-associated virus and the development of adeno-associated virus vectors: a ... trs is part of the AAV2 packaging signal Background Adeno-associated viruses (AAVs) are mammalian parvo-viruses that typically require a helper virus, such as an adenovirus or herpesvirus for productive ... synthesis of adeno-associated virus VP1 capsid protein J Virol 1988, 62:3356-3363. 18 Mendelson E, Trempe JP, Carter BJ: Identification of the trans-acting Rep proteins of adeno-associated virus...

Ngày tải lên: 12/08/2014, 01:21

... Attenuation of dengue virus infection by adeno-associated virus-mediated siRNA delivery Address: 1 Division of Allergy and Immunology-JMC Airway Disease Research Center, Department of Internal ... pellets were stored at -80°C Purification of recombinant adenoassociated viruses Cells were lysed by 5 cycles of freezing and thawing to release the virus Crude viral lysate were collected by ... smohapat@hsc.usf.edu * Corresponding author dengue virussiRNAgene expressionadeno-associated virus Abstract Background: The need for safe and effective treatment of dengue virus (DEN), a class A agent that causes...

Ngày tải lên: 14/08/2014, 19:22

... cell entry Adeno-associated virus biology Genome structure, DNA replication and virus assembly The human adeno-associated virus (AAV) was discovered in 1965 as a contaminant of adenovirus (Ad) ... author Abstract The initial discovery of adeno-associated virus (AAV) mixed with adenovirus particles was not a fortuitous one but rather an expression of AAV biology Indeed, as it came to be ... analyses of target cells and Southern blot hybridisations that 50% of infected cells were stably transduced by AAV when a multiplicity of infection of 100 was used Raising the dose of virus increased...

Ngày tải lên: 19/06/2014, 08:20

... the 3′-terminus of the virus genome, when com-pared to their arrangement in viruses of the genus Closter-oviruses Moreover, the size of CPm of GLRaV-3 is much larger than that of BYV and CTV In ... expression and cis-acting elements of Grapevine leafroll-associated virus 3 (GLRaV-3; genus Ampelovirus) was examined and compared to that of other members of the family Closteroviridae Six putative ... insects (ampeloviruses) and represent the most complex plant viruses infecting a broad range of agricul-turally important crops [2] Closteroviruses in the genera Closterovirus and Crinivirus have...

Ngày tải lên: 12/08/2014, 04:20

... of recombinant adeno-associated virus Methods Mol Med 2001; 47: 157-170 [39] Zhang X, Li CY: Generation of recombinant adeno-associated virus vectors by a complete adenovirus-mediated approach ... evolution of adeno-associated viral vectors for enhanced gene transfer Pharm Res 2008; 25: 489-499 [25] Couto LB: Preclinical gene therapy studies for hemophilia using adeno-associated virus (AAV) vectors ... RO: The role of the adeno-associated virus capsid in gene transfer Methods Mol Biol 2008; 437: 51-91 [27] Halbert CL, Allen JM, Miller AD: Adeno-associated virus type 6 (AAV6) vectors mediate...

Ngày tải lên: 05/09/2015, 11:13

... of coordinatewise negatively associated random vectors in real separable Hilbert spaces We also show that the concept of coordinatewise negative association is more general than the concept of ... the concept of negative association for random vectors in a real separable Hilbert space and develop the Baum-Katz theorem for sequences of coordinatewise negatively associated random vectors which ... > 1 of Rd-valued random vectors is NA In the following definition, we present another concept of negative association for H-valued random vectors which is more general than the concept of Ko...

Ngày tải lên: 16/10/2015, 09:29

... The patterns of topo-isomerase and RFC- 37 were the same irrespective of the Fig 1 Alkaline sucrose gradient centrifugation of viral DNA of mini-chromosomes eluted from nuclei of normoxically ... unwinding of the SV40 genome [5], an increasing amount of RPA-34 is bound to the viral minichromosome in the form of a single-stranded DNA-associated RPA trimer complex Phosphorylation of the 34-kDa ... for initiation of SV40 replication after re-oxygenation, we tested the influence of these inhibitors on the formation of SV40 form U, which Fig 4 Sensitivity of minichromosomes of hypoxic and...

Ngày tải lên: 22/02/2014, 04:20

... AccessResearch Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral ... efficiency of transduction could be increased to 35–70% by pseudotyping the retrovirus with the envelope protein of another retrovirus, Gibbon ape leukemia virus In contrast with oncoretroviruses, ... system Binding of antigen to the TCR complex results in a cascade of events com-mencing with phosphorylation of critical domains of the receptor by membrane-associated Src family of protein tyrosine...

Ngày tải lên: 19/06/2014, 08:20

... g/L of uridine, 2 g/L of glucose, 0.372 mL/L of Dawes Trace 1, 2.14 mL/L of Dawes Trace 2, 0.072 mL/L of Dawes Trace 3, 0.606 mL/L of 1 M calcium chloride dihydrate (CaCl2-2H2O), 0.30 mL/L of ... could result in a panel of reagents that will greatly improve diagnosis of Lassa fever in endemic regions of the world The classification of Lassa fever and other arenaviruses by the U.S Government ... C ELISA of purified recombinant LASV proteins using an α-LASV mAb mix Figure 4 ELISA of purified recombinant LASV proteins using an α-LASV mAb mix ELISA was performed with 100 ng of purified...

Ngày tải lên: 20/06/2014, 01:20

... fusion activity of the recombinant proteins A large number of syncytia were observed when Assembly of VLPs by expression of RVFV N and Gc proteins Figure 5 Assembly of VLPs by expression of RVFV N ... expression of the M and S segment of Hantaan virus, VLPs were assembled in mammalian cells using recombinant vaccinia virus but were not produced in insect cells with similar recombinant baculovirus ... 8Expression of N, Gn and Gc proteins produced virus-like particlesFigure 4 Expression of N, Gn and Gc proteins produced virus-like particles A) Sf9 cells were infected with the recombinant baculovirus...

Ngày tải lên: 20/06/2014, 01:20

... 14 days after administration of several different recombinant adenoviruses at a dose of 5.7 × 1012 virus particles (vp)/kg to male Sprague Dawley rats Wild type adenovirus, containing all viral ... function of CYP isforms 3A2 and 2C11 after a single dose of recombinant adenovirus serotype for a period of 14 days in the male Sprague Dawley rat [9,10] The expression and function of these isoforms, ... dose of wild type adenovirus serotype (WT), a first generation recombinant adenovirus serotype expressing E coli beta-galactosidase (AdlacZ), PEGylated AdlacZ, (PEGAd), helper-dependent adenovirus...

Ngày tải lên: 20/06/2014, 01:20

... sections of infected cells The values represent percentage of each virus form relative to the total number of virus forms counted IMV, intracellular mature virus; CEV, cell associated envel-oped virus; ... strains of vaccinia virus [7,29,30] Differences existed in the abundance of mature virus forms produced by CPXV-NOHI and progeny viruses The intracellular mature virus (IMV) is the major mature virus ... varying degrees of sta-bility of the transgene in the progeny transgenic viruses The stability of the HA + phenotype of MVA-HANP in Vero cells was not included because of low virus titres The...

Ngày tải lên: 12/08/2014, 04:21

... Integration of adeno-associated virus (AAV) and recombinant AAV vectors Annu Rev Genet 2004, 38:819-45. 8. Becerra SP, Koczot F, Fabisch P, Rose JA: Synthesis of adeno-asso-ciated virus structural ... wild type (wt) adenovirus by providing E2A, E4 and VA adenoviral genes to enable viral replication The second method offers the advantage of avoiding wt adenovirus infection and of yielding rAAV ... and organization of the adeno-associated virus 2 genome J Virol 1983, 45:555-564. 10. Trempe JP, Carter BJ: Alternate mRNA splicing is required for synthesis of adeno-associated virus VP1 capsid...

Ngày tải lên: 12/08/2014, 04:21

... sections of infected cells The values represent percentage of each virus form relative to the total number of virus forms counted IMV, intracellular mature virus; CEV, cell associated envel-oped virus; ... strains of vaccinia virus [7,29,30] Differences existed in the abundance of mature virus forms produced by CPXV-NOHI and progeny viruses The intracellular mature virus (IMV) is the major mature virus ... varying degrees of sta-bility of the transgene in the progeny transgenic viruses The stability of the HA + phenotype of MVA-HANP in Vero cells was not included because of low virus titres The...

Ngày tải lên: 12/08/2014, 04:21

... mosaic virus (BYMV), Broad bean wilt virus (BBWV), Cucumber mosaic virus (CMV),Soybean mosaic virus (SMV), Strawberry latent ring spot virus (SLRSV), Tobacco mosaic virus (TMV), Tobacco rings pot virus ... ring virus (ToBRV), Tomato ringspot virus (ToRSV), Tobacco rattle virus (TRV), Tomato spotted wilt virus (TSWV), Tobacco streak virus (TSV) (Katoch et al., 2003;Dubey et al., 2010) These viruses ... mosaic virus, CYVV= Clover yellow mosaic virus, WLMV=White lupin mosaic virus, OrMV=Ornithogalum mosaic virus, SVYV=Squash vein yellowing virus and CYNMV=Chinese yam necrotic mosaic virus Figure.1...

Ngày tải lên: 11/03/2020, 11:31