of human neural stem cells
Pharmacological inhibition of poly(ADP-ribose) polymerase-1 modulates resistance of human glioblastoma stem cells to temozolomide
... and toxicity of O6 -methylguanine, and of MGMT, responsible for the removal of the O6-methyl adduct In fact, the lack of ex-pression of one of MMR components and/or the presence of high MGMT ... densities (O D.) of the protein of interest and those of tubulin The results are representative of one out of two experiments with similar results SJ: SJGBM-2 Panel C Analysis of MGMT activity ... activity was evaluated as fmol of32P-NAD+/μg of protein MGMT activity assay and bisulfite sequencing analysis of MGMT promoter methylation Cells (1×106) were lysed in 0.5 ml of a buffer containing 0.5%
Ngày tải lên: 05/11/2020, 01:28
engraftment of nonintegrating neural stem cells differentially perturbs cortical activity in a dose dependent manner
... limi-tations of other gene delivery vehicles.2 However, primary NSC transplants, as well as NSCs derived from embryonic stem cells and induced pluripotent stem cells generate a high proportion of cells ... C17.2 cells yield high-density, titratable levels of engraftment This system pro-vides an ideal, testable model to evaluate the limits of physiologi-cal tolerance of host circuits to donor cells, ... Institute of the Children’s Hospital of Philadelphia, Philadelphia, PA, USA; 2 Department of Pediatrics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; 3 Department of
Ngày tải lên: 02/11/2022, 09:21
expansion of human mesenchymal stem cells on microcarriers
... human stem cells cannot automatically be considered to behave in a similar way to other adherent cells lines This is because with human stem cells it is the cell itself that forms the basis of ... 10.6 cells/bead; b day 2, 10.7 cells/bead; c day 3, 6.2 cells/bead; d day 4, 9.5 cells/bead; e day 5, 19.7 cells/bead; f day 7, 52.1 cells/bead; g day 8, 67.6 cells/bead; h day 9, 98.4 cells/bead; ... of human stem cells is not a trivial exercise Indeed, expanding human stem cells in culture is currently more of an art than a science so much so that some laboratories cannot or do not grow human
Ngày tải lên: 02/11/2022, 10:41
Activation of wntβ catenin signalling via GSK3 inhibitors direct differentiation of human adipose stem cells into functional hepatocytes
... differentiation of human adipose stem cells into functional hepatocytes Jieqiong Huang*, Xinyue Guo*, Weihong Li & Haiyan Zhang The generation of hepatocytes that are derived from human adipose stem cells ... presented in the cells of four groups The percentage of cells with LDL partiparti-cles in cells of Chir99021 at day 20 was relatively higher than other groups, which resembles the human hepatocytes ... (Wnt3a-treated cells), 77.1% (Chir98014-treated cells), and 72.2% (Chir99021-treated cells) (Fig. 3c), and the percentage of CXCR4 positive cells in four treated group was 72.1% (activin A-treated cells),
Ngày tải lên: 19/11/2022, 11:45
influence of aging on the quantity and quality of human cardiac stem cells
... number of CDCs for experiments, regardless of age To characterize the phenotype of CDCs, the percentage of cells positive for CD90 and CD105, which are validated markers of mesenchymal stem cells14, ... the function of cardiac stem cells In addition, these factors do not necessarily influ-ence the quantity and quality of stem cells uniformly For example, although hematopoietic stem cells from ... determinant of the quantity and quality of CDCs Senescent cells accumulate in tissues with advancing age Tissue-specific stem cells, for example those of the hematopoietic and musculoskeletal system
Ngày tải lên: 04/12/2022, 14:53
lipid mediated wnt protein stabilization enables serum free culture of human organ stem cells
... and self-renewal of multipotent human intestinal stem cells (a) Expansion of single human duodenum organoid cells (passage 7) able to form new organoids (n¼ 3, mean±s.e.m.) (b) Human duodenum organoids ... expansion of duodenum stem cells (Fig 4b) This shows that simple addition of liposomes to organoid medium enhances the stability of Wnt3a protein and allows the serum-free expansion of human organoids ... expansion of undifferentiated ESCs (Fig 2d), and higher proliferation of human duodenum organoid cells than purified Wnt3a (Fig 2e) To specifically assay for the expansion of organ stem cells, we
Ngày tải lên: 04/12/2022, 15:06
pge2 maintains self renewal of human adult stem cells via ep2 mediated autocrine signaling and its production is regulated by cell to cell contact
... PGE2-mediated novel properties of human adult stem cells using hUCB-MSCs and hAD-MSCs Results Proliferation of human adult stem cells is decreased by the inhibition of COX-2 or mPGES-1 via G1 cell ... GJIC-mediated regulation of the COX-2/PGE2 axis is not yet reported In the present study, we assessed the role of PGE2 produced by human adult stem cells in the regulation of self-renewal and immunomodulation ... autocrine effect of PGE 2 on human adult stem cells from cord blood or adipose tissue, and the regulation of its production by cell-to-cell contact, followed by the determination of its immunomodulatory
Ngày tải lên: 04/12/2022, 15:54
cellular responses to sindbis virus infection of neural progenitors derived from human embryonic stem cells
... exposure of the cells to either high concentration of gp120, or in the SGZ of gp120 transgenic mice [36,37] Thus, it is the SINV vector that disturbs Nestin expression observed in the neural stem cells ... cell responses of hESC-derived hNPCs to SINV infection demonstrated that it is a good model to study stem cell infection We are adding hNP1 cells to a novel type of human stem cells that are ... Diva software (BD Bioscience) The percentage of cells expressing fluorescence intensity greater than the control cells was calculated Proliferation assay For the assessment of the effect of SINV
Ngày tải lên: 01/11/2022, 09:05
derivation of neural progenitors and retinal pigment epithelium from common marmoset and human pluripotent stem cells
... embryonic stem cells (CESCs) closely resemble the morphology of human pluripotent stem cells (a) Colonies of CESCs grown on irradiated feeder cells (4x magnification, left) and morphology of individual ... the notion that human pluripotent stem cells assume a default neural default pathway in the absence of extrinsic explanation of lower neural commitment of cjes001 CESC into germ cells as previously ... differentiating cells formed aggregates termed embryoid bodies (EBs), consisting of cells representative of three differ-entiated germ layers 2.5 Nucleofection of HESC and HIPSCs Trypsinized human stem
Ngày tải lên: 01/11/2022, 09:48
Use of IPs cell derived neural stem cells as a cellular vehicle for glioma and breast cancer therapy
... Current status of gene therapy for glioma and breast cancer 22 1.4 Stem cells 23 1.4.1 Adult stem cells 24 1.4.1.1 Neural stem cells 24 1.4.2 Induced pluripotent stem cells 25 1.5 Stem cell ... (elaborated in section 3.1) 1.4 Stem cells 1.4.1 Adult stem cells (ASCs) Adult stem cells (ASCs), also known as somatic stem cells, are multipotent undifferentiated stem cells found in a tissue or ... Trang 11LIST OF FIGURES Figure 1 Generation of iPS cells from mouse embryonic fibroblasts (MEFs) 55 Figure 2 Generation of neural stem cells from mouse iPS cells and characterization of derived
Ngày tải lên: 09/09/2015, 18:56
Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation
... mechanisms of regulation of NSC differentiation 1.1.5.2 Pluripotent stem cell-derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blastocyst-stage ... Tables viii List of Figures ix List of Abbreviations xi Chapter 1: Introduction 1 1.1 Neural stem cells 1 1.1.1 A brief history of NSCs 1 1.1.2 The “definition” of an NSC 2 1.1.3 ... distinction of NSCs from other neural or non-neural cells, none of the markers can exclusively identify NSCs as many are also found in more committed neural progenitors, differentiated cells or
Ngày tải lên: 10/09/2015, 09:08
Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... which include issues of tumorigenicity and functionality of ESCs 2.2 Human Embryonic Stem Cells (hESCs) Embryonic stem cells (ESCs) are derived from the inner cell mass of the blastocyst-stage ... chondrogenic cells Fig 22 Tumorigenicity of hESC-derived chondrogenic cells Fig 23 ECM modulation of post-expansion differentiation capability of hESC-derived chondrogenic cells Fig 24 Response of ... temporary relief of symptoms Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs)
Ngày tải lên: 11/09/2015, 09:57
Atlas of Human Pluripotent Stem Cells doc
... culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube baby ... summarizing 12 years of our team’s experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The ... translational research in the field of embryonic and induced pluripotent stem cells It commences with practical aspects of the derivation and growth of human embryonic stem cells from inner cell mass...
Ngày tải lên: 22/03/2014, 09:20
báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... 13 The expression of miR-92 has been reported in human embryonic stem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine ... The tested adult cells were Human Microvascular Endothelial Cells (HMVEC), Human Umbilical Vein Endothelial Cells (HUVEC), Umbilical Artery Smooth Muscle Cells (UASMC), Normal human astrocytes ... undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational Medicine 2009, 7:20...
Ngày tải lên: 18/06/2014, 15:20
Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem ... mesenchymal stem cells for osteoarthritis therapy Nat Clin Pract Rheumatol 2008, 4:371-380 Estes BT, Wu AW, Guilak F: Potent induction of chondrocytic differentiation of human adipose-derived adult stem ... arcades of Benninghoff [29] and by the appearance of hypertrophic cells Analyses of lacunae surface areas in the different growth plate zones revealed mean lacunae surface areas ± SD of 100.8...
Ngày tải lên: 09/08/2014, 14:22
Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
... differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved stem cells ... differentiation of human mesenchymal stem cells into insulin producing cells J Biomed Mater Res A 2008, 86(4):1097-1105 Chao KC, Chao KF, Fu YS, Liu SH: Islet-like clusters derived from mesenchymal stem cells ... stromal cells differentiate into neural cells in vitro Exp Neurol 2000, 164(2):247-256 Lumelsky N, Blondel O, Laeng P, Velasco I, Ravin R, McKay R: Differentiation of embryonic stem cells to...
Ngày tải lên: 10/08/2014, 05:21
báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... Figure The combination of stem cell models and epigenomics in studies of the role of non-coding mutations in human disease Epigenomic analyses of cells derived through in vitro stem cell differentiation ... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine ... stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3;...
Ngày tải lên: 11/08/2014, 12:21
Engineered poly(l lactic acid) based nanofibers for osteogenic differentiation of human mesenchymal stem cells
... NANOFIBERS FOR OSTEOGENIC DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS NGUYEN THI HIEN LUONG (B.Eng., Ho Chi Minh city University of Technology, Vietnam) A THESIS SUBMITTED FOR THE DEGREE OF ... 43 2.3 Stem cells 51 2.3.1 Definition and classification 51 2.3.2 Stem cells in tissue engineering 53 2.3.3 Mesenchymal stem cells 54 v 2.4 Stem cell ... Table 2.9 Classification of stem cells [23-30] 51 Table 2.10 Advantages and disadvantages of fabrication methods of nanofibers 79 Table 2.11 Clinical trials using nanofibers 88 Table...
Ngày tải lên: 09/09/2015, 10:07
Baculovirus mediated genetic modification of human embryonic stem cells
... specific diseases in the course of ex vivo gene therapy 1.1.1 Human Embryonic Stem Cells Human embryonic stem (hES) cells are cells derived from the inner cell mass of human blastocysts The hES cell ... MODIFICATION OF HUMAN EMBRYONIC STEM CELLS DU JUAN (B Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES NATIONAL UNIVERSITY OF SINGAPORE AND INSTITUTE OF ... BACULOVIRAL TRANSDUCTION OF HUMAN EMBRYONIC STEM CELLS 11 2.1 Introduction hES cells hold great potential as a system for the study of lineage specification and as an unlimited source of cells for the regenerative...
Ngày tải lên: 11/09/2015, 14:38
The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... Adult stem cells Adult stem cells also known as somatic stem cells can be found in diverse tissues and organs The most common adult stem cells are the hematopoietic stem cells, mesechymal stem cells ... blood cells and platelets, while skin stem cells give rise to the various types of skin cells Some recent reports suggest that adult stem cells such as haemopoietic stem cells, neuronal stem cells ... of cells make up the human body viz., germ cells, somatic cells and stem cells Somatic cells include the bulk of the cells that make up the human adult and each of these cells in their differentiated...
Ngày tải lên: 16/09/2015, 15:55