nonviral vectors for cancer gene therapy

... them to serve as "Trojan horses" for the delivery of genes for cancer therapy Indeed, clostridial spores that were genetically manipulated to harbour genes for cancerstatic factors, prodrug converting ... these vectors (Table 1) Advantages of clostridial spores as "trojan horse" vectors for cancer gene therapy At present, there are various gene therapy vector systems under development against cancer ... commonly studied and most widely used system in cancer gene therapy They are of particular utility for cancer gene therapy applications, where temporary gene expression is acceptable or even beneficial...

Ngày tải lên: 14/08/2014, 19:22

... Gene therapy to inhibit pro-angiogenic pathways 12 1.1.1.6.2 Gene therapy to stimulate anti-angiogenic pathways 12 1.1.2 Gene carriers for cancer therapy 13 1.1.2.1 Viral vectors 13 1.1.2.2 Nonviral ... Abbreviations XIV Chapter Introduction 1.1 Gene therapy in cancer treatment 1.1.1 Gene therapy strategies for cancer treatment 1.1.1.1 Suicide gene therapy 2 1.1.1.1.1 Herpes Simplex Virus Thymidine ... stem cell vectors for cancer gene therapy 24 1.2.3 New reliable sources for therapeutic stem cells 26 1.3 Current mouse models in cancer research 28 1.3.1 Syngeneic models 28 1.3.2 Xenogeneic models...

Ngày tải lên: 09/09/2015, 10:08

... of vectors for gene therapy formed by self-assembly of DNA with synthetic block co-polymers Hum Gene Ther 7, 2123–2133 11 Kabanov, A V and Kabanov, V A (1995) DNA complexes with polycations for ... Molecular Medicine, vol 65: Nonviral Vectors for Gene Therapy Edited by: M A Findeis © Humana Press Inc., Totowa, NJ Maruyama and Takei lized by SECs The authors are therefore interested in HA as ... and 17 amino acid residues were sufficient to form aggregates with the DNA, From: Methods in Molecular Medicine, vol 65: Nonviral Vectors for Gene Therapy Edited by: M A Findeis © Humana Press...

Ngày tải lên: 11/04/2014, 09:59

... important for the resistance to possible introduction of genes using viral vectors or for the resistance to oncolytic gene therapy We believe that this finding can be of crucial interest for the ... vitro assay for permissivity to Adenovirus replication or transduction using a wild type or recombinant virus frequently used as oncolytic and gene therapy vectors for experimental cancer therapies ... P, Kreike B, et al: An interferon-related gene signature for DNA damage resistance is a predictive marker for chemotherapy and radiation for breast cancer Proc Natl Acad Sci USA 2008, 105:18490-18495...

Ngày tải lên: 18/06/2014, 16:20

... 1.1.2 Glioma therapy: current status and challenges 1.2 Glioma gene therapy 1.2.1 Viral vectors 1.2.2 Chemical vectors 1.2.3 Cellular vectors 10 1.3 Stem cell based glioma gene therapy 1.3.1 ... delivery vectors in gene therapy for glioma Finally, this study demonstrated, for the first time, that human embryonic stem cells can provide a potentially unlimited source for glioma gene therapy ... neural stem cell vectors in glioma gene 20 therapy 1.3.6 Stem cell based glioma gene therapy 1.3.6.1 Therapeutic genes 22 22 1.3.6.1.1 Prodrug-converting enzymes 22 1.3.6.1.2 Other gene payloads...

Ngày tải lên: 11/09/2015, 09:00

... Chapter Three: Genetic Engineering of Baculovirus Vectors for Controlled Gene Delivery…………65 3.1 Introduction………………………………………………………………… 66 3.1.1 Genetic Engineering of Baculovirus Vectors for Neuron-targeted ... Particles…………141 5.1.2 Genetic Engineering of Baculovirus Vectors for Controlled Gene Expression……………………………………………………… 142 5.1.3 Surface Modifications of Baculovirus Vectors for Improved In Vivo Gene Delivery………………………………………………… ... treatment for these difficult diseases 1.1.1 Gene Therapy The main concept of gene therapy is to use genetic materials to replace or restore function of the missing, abnormal or malfunctioning gene...

Ngày tải lên: 14/09/2015, 11:28

... different types of promoters and vectors for gene delivery to the CNS 1.1 Current progress in gene therapy Gene therapy, which usually refers to somatic gene therapy, can be broadly defined as ... used for gene therapy applications, and were rarely used for 12 Chapter Introduction gene delivery to the CNS because of their poor in vivo performance Non-viral and viral vectors are therefore ... DNA elements regulating the gene expression 11 1.4 Vectors used for gene delivery to the CNS 12 1.4.1 Non-viral vectors 13 1.4.2 Viral vectors 14 1.4.3 Hybrid viral vectors 17 1.5 Objectives of...

Ngày tải lên: 16/09/2015, 08:30

... strategy for cancer gene therapy would be the use of vectors carrying therapeutic genes that can be activated upon cellular replication This strategy may be of special clinical relevance for brain ... instable, and production 11 Table 1.1 Vectors and delivery systems for gene therapy Vectors Non-viral vectors Retrovirus Advantages • easy to produce • stable gene expression due to viral genome ... the lytic phase 1.5.1.2 HSV-1 vectors as gene delivery vehicles HSV-1 vectors are attractive vehicles for gene therapy due to their broad host range, large transgene capacity, and their ability...

Ngày tải lên: 16/09/2015, 15:54

... target for selective gene therapy As gene therapy for GBM is localised there is minimum risk of systemic toxicity (Pulkkanen and Yla-Herttuala, 2005) Gene therapy is the transfer of exogenous genes, ... in-vivo 13    1.4 Vectors for Gene Therapy Vectors which are used to carry the transgene into the tumour cells can be viral or non-viral in nature Viral vectors used in gene therapy can be replicative ... as a vector for gene therapy There is an increasing use of the insect baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-based vectors as gene delivery vectors for mammalian...

Ngày tải lên: 22/10/2015, 21:20

... paper) Gene therapy Laboratory manuals Genetic vectors Laboratory manuals Transfection–Laboratory manuals Viral genetics–Laboratory manuals I Machida, Curtis A II Series [DNLM: Genetic Vectors Gene ... Virus Type 1-Based Vectors for Gene Delivery to Human Hematopoietic Stem Cells Ali Ramezani and Robert G Hawley 467 Semliki Forest Viral Vectors for Gene Transfer Jarmo Wahlfors and Richard ... and use of viral vectors for gene transfer and gene therapy I would like to thank the many chapter authors for their contributions They are all experts in various aspects of viral vectors, and I...

Ngày tải lên: 11/04/2014, 10:39

... in activation of an oncogene, in combination with the requirement for other genetic changes before a cancer eventuates Although proviral integration can also result in gene inactivation, inactivation ... wishing to use retroviral vectors is that of insertional mutagenesis and oncogene activation Insertional mutagenesis and oncogene activation As discussed above, oncogene activation can occur ... retroviral genes Oncogene capture The mechanism of oncogene capture appears to be dependent on the generation of a chimeric retroviraloncogene transcript (69, 70) This suggests that the risk of oncogene...

Ngày tải lên: 14/08/2014, 19:22

... implications for gene therapy Gene Ther 1997; 4:258-63 Amalfitano A, Hauser MA, Hu H, et al Production and characterization of improved adenovirus vectors with the E1, E2b, and E3 genes deleted ... for generating recombinant adenoviruses Proc Natl Acad Sci U S A 1998; 95:2509-14 19 Helm GA, Alden TD, Sheehan JP, Kallmes D Bone morphogenetic proteins and bone morphogenetic protein gene therapy ... use of homologous (rat) BMP genes not improve the osteogenic potential of direct BMP adenovirus vector gene therapy, the results of this study indicate that BMP gene therapy has unique characteristics...

Ngày tải lên: 31/10/2012, 17:08

... outperform other methods in classification performance for small gene subsets while selecting genes that have plausible relevance to cancer diagnosis After formally stating the problem and reviewing ... performance for various classifiers all trained with genes selected by the method of (Golub, 1999) In contrast, the SVM selected genes yield consistently better performance than the baseline genes ... or ALL There is therefore a lot of redundancy in this gene set In essence, all the genes carry the same information Conversely, the SVM selected genes carry complementary information This is reflected...

Ngày tải lên: 06/03/2014, 00:22

... horse for vaccine development and cancer gene therapy Curr Gene Ther 2010, 10:56-76 Kim-Schulze S, Kaufman HL: Gene therapy for anti-tumor vaccination In Methods in Molecular Biology, Gene Therapy ... most common gene delivery tools used in gene therapy [37,38] The major advantage of live vectors is that they produce the antigen in its native conformation, which is important for generating ... Dendrimer-based nanoparticles for cancer therapy Nanotechnology for Hematology 2009, 708-719 64 Chatterjee DK, Zhang Y: Multi-functional nanoparticles for cancer therapy Science and Technology...

Ngày tải lên: 06/03/2014, 02:21

... a cancer gene therapy strategy Hum Gene Ther 9, 185–193 Fang J, Sawa T, Akaike T & Maeda H (2002) Tumortargeted delivery of polyethylene glycol-conjugated d-amino acid oxidase for antitumor therapy ... d-amino acid oxidase (DAAO) from Rhodotorula gracilis (EC 1.4.3.3) for cancer treatment [11] Subsequently, the strategy for cancer therapy based on oxystress and DAAO was implemented using, in addition, ... of oxystress for cancer therapy J Drug Target 15, 475–486 Niculescu-Duvaz I & Springer CJ (2005) Introduction to the background, principles, and state of the art in suicide gene therapy Mol Biotechnol...

Ngày tải lên: 16/03/2014, 02:20

... Therefore, an effective non-pharmacological therapy for cancer with less cost and no side effects could have a major impact on cancer treatment Qigong therapy from TCM shows promise in treating cancer ... rates were 83% and 17% for lung cancer patients (the control was 7% in years); 83% and 23% for stomach cancer (the control was 12% in years) The median survival period for liver cancer patients was ... anti-tumor mechanism of Qigong therapy in a study of 104 different cancer patients (mainly comprised of esophagus cancer, stomach cancer, rectum cancer and lung cancer) These patients were taught...

Ngày tải lên: 22/03/2014, 17:20

... followed by 30 cycles at 94 °C for min, 57 °C (for the 5¢Alu-PCR) or 49 °C (for the 3¢Alu-PCR) for min, and 72 °C for After this first amplification, a nested PCR was performed using 10 lL of the first ... vitro Gene Ther 4, 63–75 Pan, D., Aronovich, E.L., McIvor, R.S & Whitley, C.B (2000) Retroviral vector design studies toward hematopoietic stem cell gene therapy for mucopolysaccharidosis type I Gene ... storage in murine MPS I cells as a model for gene therapy of human MPS I ACKNOWLEDGEMENTS We thank Prof Elizabeth F Neufeld, UCLA School of Medicine, for providing us with the pBSIIKS-hIdu plasmid...

Ngày tải lên: 24/03/2014, 04:21

... highlight the relevance of CD44v isoforms in cancer which seem to be suitable targets for anti -cancer therapy In several primary and cancer cells, CD44v6 forms a ternary complex with HGF and ... Neverthe1436 less, there has been considerable interest in developing nonviral vectors for gene therapy In this regard, nonviral vectors, such as positively charged polyethyleneimine (PEI) complexes ... repair and gene therapy [106], when they are appropriately engineered for therapy We established that tissue-specific floxed plasmid ⁄ nanoparticle delivery is efficient for the activation of a gene of...

Ngày tải lên: 28/03/2014, 23:20

... into otherwise standard MuLV vectors for gene therapy has not been successful Beyond these HEMATOPOIETIC STEM AND PROGENITOR CELLS AS TARGETS FOR GENE THERAPY 137 efforts, there are obviously major ... PROGENITOR CELLS AS TARGETS FOR GENE THERAPY 143 144 GENE THERAPY FOR HEMATOLOGICAL DISORDERS restoration of cellular and humoral immunity were documented after gene therapy Data showed a surprisingly ... modified adenoviral vectors have been developed with minimal residual adenoviral genes Nonhuman marker genes such as the Neo gene or suicide genes such as tk gene included in vectors for selection...

Ngày tải lên: 10/04/2014, 22:10

... 78 VECTORS OF GENE THERAPY VIRAL VECTORS USED FOR GENE THERAPY Based on the virus life cycle, infectious virions are very efficient at transferring genetic information Most gene therapy experiments ... promoters in vivo Gene therapy with plasmid vectors has been used to attempt to treat cystic fibrosis (see Chapter 3) and cancer in humans (see Chapter 10) Risks of Nonviral Vectors for Gene Therapy There ... RETROVIRAL VECTORS 87 Use of Retroviral Vectors for Gene Therapy Retroviral vectors have been extensively used in animals and substantially used in humans to determine the efficacy of gene therapy...

Ngày tải lên: 10/04/2014, 22:11