nonviral approaches for cancer gene therapy

... as source for MSCs and NSCs in suicide gene therapy 113 5.1.1 Baculoviruses as gene therapy vectors for human cancer 113 5.1.2 HESC- derived MSCs and NSCs as gene delivery vehicles for 5.1.3 ... fusion gene approach has shown superior effects Trang 31compared to single gene expression or even the injection of two genes (Samaranayake 1.1.2 Gene carriers for cancer therapy Successful gene therapy ... CELLULAR VEHICLES FOR CANCER GENE THERAPY DANG HOANG LAM NATIONAL UNIVERSITY OF SINGAPORE 2012 Trang 2HUMAN PLURIPOTENT STEM CELL-DERIVED CELLULAR VEHICLES FOR CANCER GENE THERAPY DANG HOANG

Ngày tải lên: 09/09/2015, 10:08

... for glioma gene therapy 137 4.4 Discussion 141 Chapter 5 Conclusions 146 Trang 10SUMMARY Malignant glioma remains one of the most lethal forms of cancer in humans However, current therapy for ... vectors in gene therapy for glioma Finally, this study demonstrated, for the first time, that human embryonic stem cells can provide a potentially unlimited source for glioma gene therapy Using ... CELLULAR VEHICLES FOR GLIOMA GENE THERAPY ZHAO YING NATIONAL UNIVERSITY OF SINGAPORE 2008 Trang 2DEVELOPMENT OF NEW HUMAN STEM CELL-DERIVED CELLULAR VEHICLES FOR GLIOMA GENE THERAPY ZHAO YING

Ngày tải lên: 11/09/2015, 09:00

... Trang 1REVIEW ARTICLENanocarriers as an emerging platform for cancer therapy Nanotechnology has the potential to revolutionize cancer diagnosis and therapy Advances in protein engineering and materials ... approved for the treatment of breast cancer28 The first angiogenesis inhibitor for treating colorectal cancer, Bevacizumab (Avastin), an anti-VEGF mAb that inhibits the factor responsible for the ... therapeutics in anticancer therapy Nat Rev Cancer 2, 750–763 (2002). 17 Pastan, I., Hassan, R., FitzGerald, D J & Kreitman, R J Immunotoxin therapy of cancer Nat Rev Cancer 6, 559–565 (2006).

Ngày tải lên: 28/05/2014, 15:07

... many cancers [4] Thus, the use of gene therapy may be a new way to treat CCRCC In the field of cancer gene therapy, it is well known that the success of therapy is greatly dependent on the gene ... and does not sensitive to radiotherapy and chemotherapy [2,3] There-fore, a novel therapy strategy against CCRCC needs to be developed In the past decade, gene therapy was stu-died world-widely ... follows: first cycle at 95°C for 2 min, and then 30 cycles at 94°C for 45 s, 54°C for 1 min, 72°C for 1 min and a final extension cycle of 72°C for 5 min The house-keeping gene b-actin was taken as

Ngày tải lên: 18/06/2014, 19:20

... and thus the former is the vector of choice for future gene therapy trials The number of gene therapy trials in RA is in sharp contrast to the more than 600 clinical trials of gene therapy in patients ... the application of gene therapy in RA, and the feasibility of gene therapy was recently demonstrated in phase I clinical trials Keywords: arthritis, cytokines, gene therapy, genetic synovectomy, ... safety of local gene therapy Gene therapy in RA is still in its adolescence, and more clinical studies must be initiated if it is to mature It is, however, imperative that gene therapy studies

Ngày tải lên: 09/08/2014, 01:24

... volume for elective radiation therapy for pancreatic cancer: a pooled analysis of clinical evidence Wenjie Sun1†, Cheng N Leong2†, Zhen Zhang1*, Jiade J Lu1,2 Abstract Background: Radiation therapy ... important cancer treatment modality in both adjuvant and definitive setting, however, the use of radiation therapy for elective treatment of regional lymph nodes is controversial for pancreatic cancer ... nodal regions in conformal radiation therapy for resectable pancreatic cancer, by reviewing and summarizing the probability of lymph node metastases in resectable pancreatic cancer patients treated

Ngày tải lên: 09/08/2014, 08:23

... studies of cancers [4,11,12] have provided additional support for the exclu- sivity principle. Recent cancer genome projects have therefore evalu- ated gene sets in addition to scoring genes based ... patients one gene at the time, conventional gene set approaches are not able to differentiate between two very different scenarios. For example, consider a gene set including ten genes, and suppose ... gene-oriented approach for analysis of the same data and gene sets. For the gene-oriented approach, we started out with gene-specific scores. For each gene, the total number of mutations across all tumor

Ngày tải lên: 09/08/2014, 22:23

... The genes were then ranked according to these scores, with the most mutated genes ranked highest For each gene set, we then determined whether the ranks of the scores for the genes within that gene ... patients one gene at the time, conventional gene set approaches are not able to differentiate between two very different scenarios For example, consider a gene set including ten genes, and suppose ... gene-oriented approach for analysis of the same data and gene sets For the gene-oriented approach, we started out with gene-specific scores For each gene, the total number of mutations across all tumor

Ngày tải lên: 09/08/2014, 22:23

... Human tumorigenesis is a multistep process during which accumulation of genetic and epigenetic alterations leads to the progressive transformation of a normal cel l into a malignant cancer cell. ... gain-of-function mutations in RAS or RAF genes. Compon ents of the ERK1/2 pathway are therefore viewed as attractive candidates for the development of targeted therapies of cancer. In this article, we briefly ... et al: Activation of MEK1 or MEK2 isoform is sufficient to fully transform intestinal epithelial cells and induce the formation of metastatic tumors. BMC Cancer 2008, 8:337. 16. Scholl FA, Dumesic

Ngày tải lên: 10/08/2014, 22:20

... Aptamer ALK gene silence and Cell growth arrest B ALK gene siRN A siRNA ALCL cells ALK gene si N siR NA Figure 1 Development of a tumor cell type-selective and cancer gene-specific nanocomplex for ALCL ... study, we have described an approach for devel-oping therapeutic agent by formulating a nanocomplex that is both tumor cell-selective and cancer gene-specific for ALCL The nanocomplexes are specific ... silencing of cancerous genes by siRNAs is a potential therapeutic approach for blocking tumor growth However, siRNAs are not cell type-selective, cannot specifically target tumor cells, and therefore

Ngày tải lên: 11/08/2014, 00:22

... resuscitation Review Equipment review: The success of early goal-directed therapy for septic shock prompts evaluation of current approaches for monitoring the adequacy of resuscitation Scott R Gunn1, Mitchell ... available methods for performing measurements of gastric mucosal PCO2 in the clinical setting remain rather cumbersome and expensive Perhaps for these reasons, gastric tonometry for monitoring critically ... authors used sophisticated statistical methods for generating a cohort of study (i.e PAC) patients, each one having a paired control matched carefully for severity of illness A critical assessment

Ngày tải lên: 12/08/2014, 22:22

... them to serve as "Trojan horses" for the delivery of genes for cancer therapy Indeed, clostridial spores that were genetically manipulated to harbour genes for cancerstatic factors, prodrug converting ... commonly studied and most widely used system in cancer gene therapy They are of particular utility for cancer gene therapy applications, where temporary gene expression is acceptable or even beneficial ... vectors for cancer gene therapy At present, there are various gene therapy vector systems under development against cancer However, due to the complexity of the solid tumours involving angiogenesis,...

Ngày tải lên: 14/08/2014, 19:22

... viral gene therapy approaches Adenoviruses and Adeno-Associated viruses are used to deliver genes to tumor cells with the goal of modifying the phenotype, as for example, by introducing suicide genes ... important for the resistance to possible introduction of genes using viral vectors or for the resistance to oncolytic gene therapy We believe that this finding can be of crucial interest for the ... P, Kreike B, et al: An interferon-related gene signature for DNA damage resistance is a predictive marker for chemotherapy and radiation for breast cancer Proc Natl Acad Sci USA 2008, 105:18490-18495...

Ngày tải lên: 18/06/2014, 16:20

... vectors for gene therapy formed by self-assembly of DNA with synthetic block co-polymers Hum Gene Ther 7, 2123–2133 11 Kabanov, A V and Kabanov, V A (1995) DNA complexes with polycations for the ... Molecular Medicine, vol 65: Nonviral Vectors for Gene Therapy Edited by: M A Findeis © Humana Press Inc., Totowa, NJ Maruyama and Takei lized by SECs The authors are therefore interested in HA as ... and 17 amino acid residues were sufficient to form aggregates with the DNA, From: Methods in Molecular Medicine, vol 65: Nonviral Vectors for Gene Therapy Edited by: M A Findeis © Humana Press...

Ngày tải lên: 11/04/2014, 09:59

... outperform other methods in classification performance for small gene subsets while selecting genes that have plausible relevance to cancer diagnosis After formally stating the problem and reviewing ... performance for various classifiers all trained with genes selected by the method of (Golub, 1999) In contrast, the SVM selected genes yield consistently better performance than the baseline genes ... or ALL There is therefore a lot of redundancy in this gene set In essence, all the genes carry the same information Conversely, the SVM selected genes carry complementary information This is reflected...

Ngày tải lên: 06/03/2014, 00:22

... horse for vaccine development and cancer gene therapy Curr Gene Ther 2010, 10:56-76 Kim-Schulze S, Kaufman HL: Gene therapy for anti-tumor vaccination In Methods in Molecular Biology, Gene Therapy ... Dendrimer-based nanoparticles for cancer therapy Nanotechnology for Hematology 2009, 708-719 64 Chatterjee DK, Zhang Y: Multi-functional nanoparticles for cancer therapy Science and Technology ... nanoparticles for the detection, analysis and treatment of cancer have been mentioned Many applications in vaccine therapy or gene therapy are listed as following: Bolhassani et al Molecular Cancer...

Ngày tải lên: 06/03/2014, 02:21

... suicide gene therapy Antiangiogenic gene therapy Virotherapy Virotherapy Measles virus Virotherapy Measles and mumps viruses Virotherapy Adenovirus AAV Delivery of p73 HSV-TK suicide gene therapy ... recurrent ovarian cancer Cancer Gene Ther 9: 553 – 566 Hasenburg, A., et al (2000) Thymidine kinase gene therapy with concomitant topotecan chemotherapy for recurrent ovarian cancer Cancer Gene Ther ... study of adenovirus–p53 gene therapy for recurrent ovarian cancer Cancer Gene Ther 10: 224 – 238 Zeimet, A G., and Marth, C (2003) Why did p53 gene therapy fail in ovarian cancer? Lancet Oncol 4:...

Ngày tải lên: 22/03/2014, 11:20

... Therefore, an effective non-pharmacological therapy for cancer with less cost and no side effects could have a major impact on cancer treatment Qigong therapy from TCM shows promise in treating cancer ... rates were 83% and 17% for lung cancer patients (the control was 7% in years); 83% and 23% for stomach cancer (the control was 12% in years) The median survival period for liver cancer patients was ... anti-tumor mechanism of Qigong therapy in a study of 104 different cancer patients (mainly comprised of esophagus cancer, stomach cancer, rectum cancer and lung cancer) These patients were taught...

Ngày tải lên: 22/03/2014, 17:20

... of target gene expression or gene silencing can also be performed using miRNA, siRNA or shRNA expression vectors [1] Gene therapy is useful for both genetic and acquired diseases For genetic diseases, ... Introduction Gene therapy is a promising approach to treat intractable and refractory diseases at the genetic level Basically, in gene therapy, target gene expression is induced by delivering foreign genes ... fibrosis [3] Gene therapy clinical trials were also performed for acquired diseases such as cancers, cardiovascular diseases and infectious diseases [3] There are two strategies to perform gene therapy, ...

Ngày tải lên: 23/03/2014, 14:20