nkt cells in periodontitis lesions

... on the cells recovered from nitrocellulose filters Using this stain one can discriminate between cells that lost membrane integrity, stain red and are dead, while intact viable cells stain green ... estimated the surviving cells using direct counting techniques including the live/dead stain Based on differences in results between these enumeration techniques, the results indicate that only ... Thus, when integrating different time points the CFU declined steadily, while the fraction of viable cells remained similar over time A Trang 12regression line describing the deduced increase of

Ngày tải lên: 20/06/2014, 20:20

... methanol for 10 min Cell monolayers were blocked in 5% BSA in PBS for 45 min and incubated for 1 h at room temperature with P-gp antibodies (Invitrogen, Beijing, China), followed by incubation for ... Southeast inocu-lated into the right flank of mice using a 1.0 mL syringe Intravenous injection of reagents and tumor growth inhibition study The nude mice inoculated with HepG2/ADM cells were ... uptake of the DNR into HepG2/ADM cells Staining and flow cytometry analysis to detect apoptosis Using acridine orange/ethidium bromide (AO/EB) dye mixture staining for apoptotic cells, apoptotic

Ngày tải lên: 21/06/2014, 03:20

... processes were intermingled with other IR cells especially with somatostatin-IR cells (Figs 2ac) In the pancreatic duct, spindle shaped insulin-IR cells were detected in the inter-epithelial cells of ... gastrin, chole-cystokinin, secretin, glucagon, insulin, motilin and gastric acid [26] and absorption of amino acid, glucose and fatty acid in the gastrointestinal tract [6] Somatostatin-IR cells ... dispersed mainly in the central region, intermingled with insulin-IR cells [44, 50] However, Yoshida et al [55] suggested that somatostatin-IR cells occur in the peripheral regions of islets intermingled

Ngày tải lên: 07/08/2014, 15:20

... effective in canine SCI model, there was little histological evidence of spinal cord tissue regeneration [22] In this study, we examined whether canine ASCs could survive and integrate into neural cells ... hematoxylin and eosin, and then with combined Luxol fast blue and cresyl violet to identify myelin and nerve cells [10] Percentages of myelinated areas in damaged spinal cords were calculated using ... adipose-derived stem cells (ASCs) improved neurological function in a canine spinal cord injury model Eleven adult dogs were assigned to three groups according to treatment after spinal cord injury by

Ngày tải lên: 07/08/2014, 23:22

... lining cells, are now thought to be responsible for initiating and sustaining the inflammation The re-emer-gence of the notion that cells of the innate immune system are essential in generating ... synovial lining cells, B cells and T cells in the pathogenesis of CIJD (for a recent review on this, see [2]) 3 The receptor system, implied by our findings, is present on synovial lining cells, B cells ... involved in the pathogenesis and severity of inflammatory arthritis [12,13] T cells of multiple specificities, including self-specificities, are a frequent finding in inflammatory joint diseases

Ngày tải lên: 09/08/2014, 01:23

... washing buffer including PBS, EDTA and BSA sep-arated by FACS sorting Sorted cells (1000) were incu-bated with 500,000 autologous antigen-presenting cells in the presence of 20 U/ml interleukin ... peptide-specific T cells were detected with phycoerythrin (PE)-labelled HLA-B27 tetramer (a) and 0.66% of CD8+ T cells by performing intracellular cytokine staining of IFN-γ-secreting cells (b) After ... stained with 5 µg/ml anti-CD69-PE antibody (BD Pharmingen) and 1 µg/ml anti-CD8-PerCP (BD Pharmin-gen) Cells were then fixed in 2% formalin and resus-pended in saponin buffer, followed by incubation

Ngày tải lên: 09/08/2014, 01:24

... cells are sentinels of the immune system, detecting and delivering a first response to invading bacteria and other insults Accumulating within inflamed tissues, mast cells produce cytokines and other ... migration up chemotactic gradients into the joint The composition of inflammatory cells recruited in a continuing fashion by mast cells, including the degree of skewing of lymphocytes toward Th1 versus ... functional contribution of mast cells to continuing inflammation remains to be experimentally determined In humans, given the expanded numbers of mast cells within the joint and their enormous capacity

Ngày tải lên: 09/08/2014, 01:24

... cells were labelled with 5,6-carboxyfluorescein diacetate succinimidyl ester (CFSE) and injected intravenously After 24 hours single-cell suspensions from draining lymph node (dLN), nondraining ... and GITR-ligand interactions, abrogating the suppressive effect of Treg cells [37] With this in mind, it could be interesting to investigate whether the accumulated Treg cells in patients with ... allografts [28], in Langerhans islets and pancreatic lymph nodes in inflammation-induced diabetes [29], in chronically inflamed skin in a Leishmania infection model [30], and in the mucosa and

Ngày tải lên: 09/08/2014, 06:22

... liposomes were injected s.c in the hind paws, the majority of the CD4+CD134+ T cells in the joint-draining PLN could indeed be targeted The non-T cells in the PLN that were found to bind both anti-CD134 ... drug-containing anti-CD134 liposomes Next, we investigated whether local targeting to CD134+ T cells in the joint-draining PLN would affect the course of actively induced arthritis in the AA ... This indicated that the absolute number of CD134+CD4+ T cells decreased during the interval from day 7 to day 10 In the spleen, the main increase in the percentage of CD134-expressing T cells

Ngày tải lên: 09/08/2014, 06:22

... performed in 22 points inside the high dose/low gradient PTV region (15 points for med-iastinic lesion, 4 and 3 points for single and multiple metastasis, respectively) and in 27 points (15 for ... point dose measurements can be performed both in all tumor inserts and in the homogeneous mediastinum region, thanks to several inserts created inside the phantom Trang 32.2 Treatment planningFor ... considering the g-area histograms and distribution using the Tomotherapy Inc software (Research station), by con-sidering all the points of the film that are included in the homogeneous/inhomogeneous

Ngày tải lên: 09/08/2014, 09:20

... cells in the inflamed glands Glandular B cells expressing distinct features of IgV light and heavy chain rearrangements, (re)circulating B cells with increased mutations of cµ transcripts in both ... frequencies of individual peripheral B cells containing IgV heavy chain transcripts of multiple isotypes indicate disordered selection and incomplete differen-tiation processes of B cells in the inflamed ... cytokines and chemokines including a striking overexpression of the B-cell-attracting chemokine CXCL13 (BCA-1) and, to a smaller degree, CXCL12 (SDF-1) [28-31] Accordingly, B cells expressing

Ngày tải lên: 09/08/2014, 10:20

... percentage was determined in rela-tion to the initial 1 × 106 plated cells Di-I-acetylated low density lipoprotein uptake and Ulex staining PBMNCs were suspended in culture medium Cells (4 × 105) ... amino-terminal two repeats of the death effector domain (DED), it lacks the carboxy-terminal half of the proteolytic domain Caspase 8L binds to the Fas-asso-ciating protein with death domain ... smaller in size and contained less emerging, spindle-shaped cells Van Beem and colleagues [41] have shown that these CFU and the spindle-shaped emerging cells are monocyte-derived CD14+ cells but

Ngày tải lên: 09/08/2014, 10:20

... arthritis Introduction T cells are likely to play an important role in the pathogenesis of rheumatoid arthritis (RA) (reviewed in [1]) In the synovial joint, infiltrating T cells are predominantly ... be excluded using the combination of cells The density of CD4+CD28null T cells were calculated by dividing the number of CD4+CD28null T cells by the total area of infiltrating T cells, measured ... patients analysed, the dominant TCR-Vβ withinalso present in the joint In one such representative patient, cells in the circulation, in the left knee synovial fluid and in the right knee synovial

Ngày tải lên: 09/08/2014, 10:21

... The clinically observed increase in tumor control after combination of goserelin with radiotherapy in PC cannot be attributed to an increase in radiosensitivity of PC cells by goserelin in vitro ... line Washing and incubation with HFM was done in the maintenance cul-tures After 24 h cells were detached using trypsin/EDTA, counted, diluted and replated in 25 cm2 flasks in FMH + goserelin ... staining Changes in protein expression To test the functional activity of goserelin in the investi-gated cell lines, we analyzed protein expression and phos-phorylation that are involved in functional

Ngày tải lên: 09/08/2014, 10:21

... seen in the cells of a plexiform lesion (P) Inset exhibits prominent luminal staining of GDF-15 in cells lining the vascular channel (arrow) Note the presence of GDF-15 in the endothelial cells ... signal for GDF 15 is noted in cells lining vascular channels Inset shows prominent luminal staining of GDF-15 in endothelial cells (arrowhead) Note lower signal for GDF-15 in the connective tissue ... detected in the smooth muscle cells of remodeled pulmonary arteries (Figure 3) In concentric lesions GDF-15 expression was noted in cells lining the small lumen of lesions, probably endothelial cells...

Ngày tải lên: 12/08/2014, 13:22

... heating channels in the jacket, through the highly conductive jacket material, and into the core We can calculate the temperature profile by defining a heat balance in the reactor, assuming that ... production in future cars, is the new generation of fuel cells These work principally as batteries do, yet while batteries can be considered as batch reactors, fuel cells are continuous reactors In a ... combination of modeling and experiments has shown to lower costs and accelerate the pace of building prototypes and understanding of these new systems The optimization of the fuel cell, in combination...

Ngày tải lên: 19/10/2013, 09:15

... shown that interactions between KCs and endotoxin comprise the initiating event leading to hepatotoxicity in liver injury, including endotoxemia and ischemia ⁄ reperfusion injury [14] In our studies, ... Regulation of hepatic drug-metabolizing enzyme genes by Toll-like receptor signaling is independent of Toll-interleukin receptor domain-containing adaptor protein Drug Metab Dispos 36, 95–101 10 ... numerous procarcinogens, including aflatoxin B1 [21] In the present study, CYP1A1 and 1A2 activities were significantly decreased, with a concomitant decrease in their protein levels during the late...

Ngày tải lên: 14/02/2014, 18:20

... cyclase, forskolin induces increased levels of cAMP in treated cells This in turn activates cAMP-dependent protein kinase A (PKA) which phosphorylates CREB and other target proteins Wortmannin A mM stock ... obtained after irradiating cells with UV light are in perfect agreement with the immunoblot analyses and suramin inhibition experiments presented in our study indicating an auto- and/or paracrine ... phosphatidylinositol 3-kinase (PtdIns3-kinase, IC50 ˆ nM) wortmannin allows us to investigate downstream signalling of the PtdIns3 pathway and dependent activation events Suramin Suramin (Sigma, St Louis,...

Ngày tải lên: 17/03/2014, 17:20

... polyadenylated during meiotic maturation and binds CPE-binding protein (CPEB), a highly conserved RNA-binding protein with both a zinc finger and an RNA-recognition motif (RRM) (53,54) Injection into Xenopus ... stored mRNAs RINGO/Spy protein is absent in the oocyte cytoplasm, while its mRNA is maintained in a dormant state This is achieved by binding of Pumilio-2, an RNA-binding protein initially described ... cap-binding factor The binding of maskin to eIF4E precludes the binding of eIF4G to eIF4E, thus inhibiting the formation of the translation initiation complex Following stimulation, the kinase...

Ngày tải lên: 22/03/2014, 20:21

... declined in gastric cancer [35], we speculated that PTEN might participate in the VD3-induced apoptosis in gastric cancer cells To investigate this, undifferentiated HGC-27 adenocarcinoma cells ... were therefore interested in determining whether TSA ⁄ NaBu had any effects on promoting the binding of Egr-1, a major transcription factor of PTEN To this, we first examined the in uences of TSA ... transfected cell lines HGC-27 cells were transfected with pSliencer 4.1–CMV neo-siPTEN or pSliencer 4.1–CMV neo vector, using the Lipofectamine 2000 (Invitrogen) protocol Cells were maintained in IMDM...

Ngày tải lên: 22/03/2014, 21:20