molecular techniques and applications methods in molecular biology vol 666

... 12Brad M Binder and G Eric Schaller (eds.), Ethylene Signaling: Methods and Protocols, Methods in Molecular Biology, vol 1573, DOI 10.1007/978-1-4939-6854-1_1, © Springer Science+Business Media ... Yoon and Yi-Chun Chen Trang 20Brad M Binder and G Eric Schaller (eds.), Ethylene Signaling: Methods and Protocols, Methods in Molecular Biology, vol 1573, DOI 10.1007/978-1-4939-6854-1_2, © Springer ... synthesis in plants Nature 286:256–260 Bram Van de Poel and Dominique Van Der Straeten Trang 36Brad M Binder and G Eric Schaller (eds.), Ethylene Signaling: Methods and Protocols, Methods in Molecular...

Ngày tải lên: 13/05/2017, 21:17

... establish clean point mutations and in-frame deletion mutations that have been instrumental in identifying and understanding the molecular interactions between components of the Yersinia type III ... dendritic cells This interaction causes an increase in pro-infl ammatory cytokines and chemokines The increase in infl ammation eventually leads to edema, erythema, abscess formation, and mucosal hemorrhages ... synthesis and secretion translocators have been studied in some detail Utilizing in-frame deletion mutagenesis uncovered two LcrH-interacting domains in YopD—the fi rst being a large N-terminal domain...

Ngày tải lên: 25/12/2016, 15:05

... as insulin-like growth factor-binding proteins (IGFBPs) [ 23 ] and interleukin-6 (IL-6) (reviewed in ref 24 ) These observations indicate that senescence is not just dictated by events happening ... Nikiforov (ed.), Oncogene-Induced Senescence: Methods and Protocols, Methods in Molecular Biology, vol 1534, DOI 10.1007/978-1-4939-6670-7_1, © Springer Science+Business Media New York 2017 ... gelatin and let coverslips dry before cell plating 7 Cells should always be plated in duplicate for experimental replicates 8 SA-βgal staining should be performed in the evening Staining...

Ngày tải lên: 25/12/2016, 15:07

... and 2 (HTLV-1 and HTLV-2) infections in HTLV seroindeterminate individuals from Sao Paulo, Brazil J Clin Virol 44(3):185–189 80 Garin B, Gosselin S, de The G, Gessain A (1994) HTLV-I/II infection ... HTLV indeterminate pattern provisionally called “New HTLV indeterminate” indeterminate pattern (T–Z) HTLV-1 Infection Epidemiology and Diagnosis Trang 25discriminate between HTLV-1 and HTLV-2 infections ... protein (GD21) of HTLV-1 and HTLV-2 as well as specific synthetic peptides originating from HTLV-1 (including MTA-I or rgp46-I) and HTLV-2 (including K55 or recognize both HTLV-1 and HTLV-2 infection...

Ngày tải lên: 13/05/2017, 21:19

... 30Ingrid C Gelissen and Andrew J Brown (eds.), Cholesterol Homeostasis: Methods and Protocols, Methods in Molecular Biology, vol 1583, DOI 10.1007/978-1-4939-6875-6_3, © Springer Science+Business ... Cholesterol-Binding Domains in Membrane Proteins Coralie Di Scala and Jacques Fantini Abstract In eukaryotic cells, cholesterol is an important regulator of a broad range of membrane proteins, including ... 1 Ingrid C Gelissen and Andrew J Brown 2 Hybrid In Silico/In Vitro Approaches for the Identification of Functional Cholesterol-Binding Domains in Membrane Proteins 7 Coralie Di Scala and...

Ngày tải lên: 13/05/2017, 21:19

... Snick J (2006) Allergen- induced interleukin-9 production in vitro: correlation with atopy in human adults and comparison with interleukin-5 and interleu-kin- 13 Clin Exp Allergy 36(2):174–182 ... chain and common γ chain [13] Signal transduction of IL-9 leads to activation of multiple STAT molecules including STAT1, STAT3, and STAT5 and MAPK and IRS-PI3K pathway [14–16] T Cell lines and ... Study of Inflammatory Diseases in Research and Clinical Settings 247 Patricia Keating and James X Hartmann Index 257 Contents Trang 8Silvia C.P almeida • Faculdade de Medicina, Instituto...

Ngày tải lên: 13/05/2017, 21:20

... possession an ever-expanding arsenal that continues to aid us in dissecting the function of telomerase and telomere-binding proteins, probing the changes in telomeres, and elucidating the consequences ... transfer and minimize bubbles 9 The membrane may be further incubated in NaOH solution for 5–10 min to denature any remaining DNA, neutralized again, and rinsed in 2× SSC before cross-linking 10 ... (2001) The Pin2/TRF1- interacting protein PinX1 is a potent telomer- ase inhibitor Cell 107:347–359 49 Baumann P, Cech TR (2001) Pot1, the tive telomere end-binding protein in fission yeast and humans...

Ngày tải lên: 13/05/2017, 21:21

... of interest 2 Hongseok Ha et al. Trang 13For studies involving multiple samples, Illumina provides6 bp index sequences for pooling multiple samples in one ing library We tested 48 indexes and ... blocking during runs 16 If tips are to be used again within 24 h they can remain in thetip holder and be stored in the fridge, inside the black box.Ensure that there are some volumes of NDTs remaining ... solution obtained homoge-22 Gwen Hoad and Kristina Harrison Trang 326 Prepare Binding Buffer/Streptavidin Sepharose bead mix.38 μL Binding buffer and 2 μL beads are required per well.Prepare volume...

Ngày tải lên: 13/05/2017, 21:21

... obtain a large amount of quantitative information on immune cell migration and interactions including the velocity, meandering index, turning angles, duration and timing of interactions, and ... of Birmingham, Birmingham, UK and Molecular Biology, The University of Texas at Austin, Austin, TX, USA London, UK and Inflammation, The University of Glasgow, Glasgow, UK Switzerland and Inflammation, ... Trafficking: Methods and Protocols, Methods in Molecular Biology, vol 1591, DOI 10.1007/978-1-4939-6931-9_1, © Springer Science+Business Media LLC 2017 Key words Multiphoton, Modeling, 3D imaging,...

Ngày tải lên: 13/05/2017, 21:21

... Contributors Trang 11Jing Lin and Marcos Alcocer (eds.), Food Allergens: Methods and Protocols, Methods in Molecular Biology, vol 1592, DOI 10.1007/978-1-4939-6925-8_1, © Springer Science+Business Media ... immunodetection and providing certain discriminating character-istics such as isoelectric points or molecular mass Using 2DGE, proteins are fractionated according to their isoelectric points in the first ... with five volumes of binding buffer (5 mL) at a flow rate of 2 mL/min 4 Elute with a linear gradient from 0 to 0.5 M NaCl in binding buffer in 15 column volumes (15 mL) Fraction containing purified...

Ngày tải lên: 13/05/2017, 21:22

... of four zinc fingers and a low- complexity activation domain, both of which are required for pro-moting DNA binding and mediating transcriptional activation During ZGA, Vfl/Zld-binding sites ... novel binding sites using structural information RBPmap [ 58 ] Scan for known RNA-binding motifs RNAcontext [ 59 ] Discovery of novel binding sites using structural information MEMERIS [ 60 ] Incorporates ... Activation: Methods and Protocols, Methods in Molecular Biology, vol 1605, DOI 10.1007/978-1-4939-6988-3_2, © Springer Science+Business Media LLC 2017 Chapter 2 Link of Zygotic Genome Activation and...

Ngày tải lên: 13/05/2017, 21:24

... Protein-Ligand Binding Sites and Structures 97Hui Sun Lee and Wonpil Im 9 Local Alignment of Ligand Binding Sites in Proteins for Polypharmacology and Drug Repositioning 109Michal Brylinski ... query protein structure,including global and local structure matching to known proteins Chapter8describes G-LoSA, which finds ligand binding sites similar to a query binding site within a referencedatabase ... (ed.), Protein Function Prediction: Methods and Protocols, Methods in Molecular Biology, vol 1611, DOI 10.1007/978-1-4939-7015-5_1, © Springer Science+Business Media LLC 2017 1 Trang 12Hawkins &...

Ngày tải lên: 13/05/2017, 21:25

... contain-ing ampicillin with an individual bacterial colony and incubate at 37 °C overnight in a shaker incubator (240 rpm) Purify the plasmid DNA using QIAprep Spin Miniprep Kit according to ... Viruses: Methods and Protocols, Methods in Molecular Biology, vol 1602, DOI 10.1007/978-1-4939-6964-7_2, © Springer Science+Business Media LLC 2017 gen-Key words Equine arteritis virus, EAV, Equine ... directly into the tube containing TransIT-LT1/OPTI-MEM I and mix by pipetting gently or vortexing for 2 s Incubate mixture at RT for 30 min 5 Remove medium from tissue culture dish containing attached...

Ngày tải lên: 16/05/2017, 23:21

... Netherlands Trang 12Andrew F Hill (ed.), Exosomes and Microvesicles: Methods and Protocols, Methods in Molecular Biology, vol 1545, DOI 10.1007/978-1-4939-6728-5_1, © Springer Science+Business ... scattered light contains information, ing detection and analysis of the particles which is employed in common and new techniques based on light scattering allow-Bearing in mind the principle of energy ... sensing zone of the Andrew F Hill (ed.), Exosomes and Microvesicles: Methods and Protocols, Methods in Molecular Biology, vol 1545, DOI 10.1007/978-1-4939-6728-5_2, © Springer Science+Business...

Ngày tải lên: 24/12/2016, 22:31

... SY5Y and 5-day-old mouse brain were examined using 10% Tris/tricine gels with reducing Ó FEBS 2003 Analysis of mouse and human brain presenilin complex (Eur J Biochem 271) 381 Fig PEN-2 and APH-1 ... 10-kDa band in PS1 wild-type neural stem cells, E15 mouse brain and SY5Y cells Low-level expression was present in PS1 deficient cells, adult mouse brain and in control and AD human brain cortex ... H.T., Tandon, A., Fraser, P.E & St George-Hyslop, P (2003) APH-1 interacts with mature and immature forms of presenilins and nicastrin and may play a role in maturation of presenilin.nicastrin complexes...

Ngày tải lên: 16/03/2014, 16:20

... a kinase In many signal-transducing protein kinases, site-specific phosphorylation by an upstream protein kinase (a kinasekinase) activates them Before these activation steps, the protein kinases ... signaling protein kinases Cdc37 phosphorylation by CK2 and signaling kinases to Cdc37, we next investigated whether the CK2-phosphorylated form of Cdc37 associated with signaling protein kinases in ... Cdc37 band appearing within (Fig 8B, lane 8) Again, the band shift induced was direct, from the low-mobility to high-mobility bands, with no intermediate bands being detected Analysis of signaling...

Ngày tải lên: 30/03/2014, 03:20

... Staining: The most sensitive staining for protein is silver staining This involves soaking the gel in Ag NO3 which results in precipitation of metallic silver (Ag0) at the location of protein ... identification /finger printing of intact proteins or of proteins digested in gel slices Since gels used in biochemistry are chemically rather unreactive, they interact minimally with biomolecules during ... improve methods allowing unveil rotavirus co-infections in future studies These findings would be of interest in order to increase current knowledge about rotavirus evolution and determine the...

Ngày tải lên: 28/06/2014, 15:20

... many amino acid substitutions McLellan and Sherman (1991),using non-denaturating electrophoresis, reported that from 40- 57% of proteins differing by single amino acids can be separated on a single ... of the best methods for distinguishing among protein molecules (McLellan and Inouye, 1986) In the same way, isoelectric focusing (IEF), polyacrylamide gel electrophoresis (PAGE) and 2dimensional ... system, single zone of activity was evident in the species Staining intensity and resolving power were poor for T superba The R of ’s f a the observed zone were 0.36 and 0.40 for Using the H...

Ngày tải lên: 08/08/2014, 23:22

... availability during the plant growth, in the protein profiles of both organs In order to obtain further information, in this work we investigated protein accumulation changes induced by nitrate in both ... total proteins, amino acids, reducing sugars and sucrose in roots (A, C, E and G) and leaves (B, D, F, and H) and chlorophyll content in leaves (I) of Zea mays plants, previously grown in the absence ... Healthcare) Determination of reducing sugars, sucrose, amino acids, total proteins and chlorophyll Reducing sugars, sucrose and amino acids were extracted by homogenizing frozen tissues in volumes of...

Ngày tải lên: 12/08/2014, 03:21

... against viral infection Others Rho GTPase activating protein 29 (PARG1, ARHGAP29), encoding for a protein-tyrosine phosphatase-associated Rho GTPase activating protein, is involved in signaling ... and utilize host cytoskeleton to promote viral infection like many other viruses[28,29] FLNA is an actin-binding and signal mediator scaffolding protein that crosslinks actin filaments and links ... control and experimentally infected pigs were processed routinely for haematoxylin and eosin (H&E) staining and were re-isolated of H-PRRSV and N-PRRSV viruses and then were identified by IFA and...

Ngày tải lên: 12/08/2014, 04:20