molecular methods for bacterial strain typing

... of SBV ···3 4 Purpose of this study ···3 II Materials and methods ···4 1 Bacterial strain and plasmid vector for molecular cloning 4 2 Bacterial culture medium ···5 3 SBV-infected honeybee samples ... resistance to SBV might be found.II Materials and methods 1 Bacterial strain and plasmid vector for molecular cloning The Escherichia coli (E coli) strain DH5αF’, genotype F´/endA1 hsdR17(rK– mK+) ... diluted to 1× for thefinal use III Results and discussion 1 Standard DNAs for SBV genotyping The standard DNAs of 5 genotypes were constructed, and used aspositive control for genotyping identification

Ngày tải lên: 21/05/2020, 11:16

... seconds for stable amplification and to save the total time required for performance The denaturation time was set at 1 second for the lowest temperature and time of performance The final performance ... dataset revealed that the closest relationship was found for strain AmSЬѴ-K̟0г21 (JQ390591) for 2100D0, and strain AЕSЬѴ-K̟0г4 (K̟Ρ296803) for 2134D51 The number of nucleotides was traced from the ... coli (E coli) strain DH5αF', known for its high transformation efficiency, was widely utilized for cloning purposes Plasmid blueXem, derived from Bluescript II KS (+), was employed for cloning

Ngày tải lên: 24/07/2023, 17:04

... Methods for adjusting ambient air quality levels for meteomlogical effects an discussed by Zeldin and Meisel (1978) 16.3 METHODS 16.3.1 Graphical Graphical methods are very useful aids to formal ... in the pth group For example, in the sequence (23, 24, hace, 6, trace, 24, 24, trace, 23) we have g = 3, I, = 2 for the tied value 23, I, = 3 for the tied value 24, end t, = 3 for the three trace ... In this tia an we review these problems, m g g a appmaehes for Unir alleviation, and reference pertinent literature for additional information Hamed d al (1981) review the literature dealing with

Ngày tải lên: 18/02/2014, 04:20

... to the standard formula for the Newton iteration x+= x c − F  (x c)−1 F (x c ). (2.2) One could say that Newton’s method for unconstrained optimization is simply the method for nonlinear equations ... search globalizations of the BFGS quasi-Newton method for unconstrained and bound constrained problems We refer the reader to the literature for more general results Our intention is that both ... ≤ f(x) for all x, (1.3) is sought The constrained optimization problem is to minimize a function f over a set U ⊂ R N A local minimizer, therefore, is anx ∗ ∈ U such that f(x ∗ ) ≤ f(x) for all

Ngày tải lên: 21/02/2014, 09:20

... Incorporating Non-local Informa-tion into InformaInforma-tion ExtracInforma-tion Systems by Gibbs Sampling In Proceedings of the Annual Meeting of the Association for Computational Linguistics ... LEX-PRED.6 For this learning method, we use CRF++.7 We choose a configuration that provides good perfor-mance on our source domain (i.e ETHICS).8 For semantic role labeling we use SWIRL9, for chunk ... for chunk parsing CASS (Abney, 1991) and for con-stituency parsing Stanford Parser (Klein and Man-ning, 2003) Named-entity information is pro-vided by Stanford Tagger (Finkel et al., 2005) 4.2 Convolution

Ngày tải lên: 22/02/2014, 02:20

... components of strain and the displacement components are au, dw, buaU , aor | wow 5z lấy Í Scar + eax t oe ox For small deformations, the strain-displacement reli ‘the expressions for the strain ... Theory 11 22.2 Strain Components Corresponding to the six stress components, the state of strain ata point can be divided into six strain components, In a vector form, the state of strain is expressed ... ¢¢ = components of normal strains ‘Yer Yrs and Yor = components of shear strains The notation and sign convention used for the strain components are the same as those for the stress components,

Ngày tải lên: 23/02/2014, 21:20

... Toxicity DRAFT FOR CONSULTATION 14.07.10 263 The test may be particularly useful as part of the initial screening for the assessment of 264 chemicals for which little or no toxicological information ... any combination of alternative approaches 1026 Therefore, for reproductive toxicity testing, animal studies will remain the last resort for 1027 foreseeable time This is caused by e.g the often ... screening and SAR efforts Expert Opinion on Drug Discovery 2010;5:21-31 [90] Enoch SJ, Cronin MTD, Madden JC, Hewitt M Formation of structural categories to allow for read-across for teratogenicity

Ngày tải lên: 05/03/2014, 16:20

... Performance Tests10.1 At the beginning of each day that analyses are to be performed, the GC/MS system must be checked to see if acceptable performance criteria are achieved for BFB The9performance ... correction will be formalized through a rulemaking in FY97 Equation 1 where: A = Area of the characteristic m/z for the parameter to be measured.s A = Area of the characteristic m/z for the internal ... established performance criteria to determine if the results of analysesmeet the performance characteristics of the method When results of sample spikesindicate atypical method performance, a quality

Ngày tải lên: 05/03/2014, 21:20

... concentration foreach compound and internal standard Calculate response factors (RF) for eachcompound using Equation 1 Equation 1 where: A = Response for the parameter to be measured.s A = Response for ... of wastewaters for derivatization products ofthe parameters listed in the scope (Section 1.1), and was used to develop themethod performance statements in Section 14 Guidelines for the use of ... standards If the response for anyparameter varies from the predicted response by more than ±15%, a new calibrationcurve must be prepared for that compound 7.5 To calibrate the ECDGC for the analysis of

Ngày tải lên: 05/03/2014, 21:20

... control Forexample, the year 2000 target rate (per 100,000)for cancer mortality was 130 for the total population, but it was 175 for blacks The implication of setting different targetsfor different ... transformation of the dependent variable yi.For transformations of the dependent variable yi(e.g., the logarithmic or logit transformation),β 1then becomes a relative-risk (logarithmictransformation) ... the last decade, focus on and efforts topromote population health data for major ethnicgroups have increased Ten-year trends now areavailable for some groups For subgroups withinmajor racial/ethnic

Ngày tải lên: 06/03/2014, 01:20

... 2009 FEBS W.-J Xu et al Methods for 3¢ end amplification from SAGE tags A B Fig Schematic for GLGI (A) In this process, first-strand cDNA synthesized by oligo(dT) is used for PCR In the first cycle, ... only for the subsequent PCR but also for 5¢-RACE, 3¢-RACE and northern blotting, etc Finally, the two-step nested PCR principle can easily produce 3¢-end cDNA tag-specific frag- Methods for 3¢ ... approaches for transcriptome research and genome annotation Nat Methods 2, 495–502 FEBS Journal 276 (2009) 2657–2668 ª 2009 The Authors Journal compilation ê 2009 FEBS 2663 Methods for 3Â end

Ngày tải lên: 07/03/2014, 00:20

... a molecular chaperone [5] PDI is thought to bind poly-peptides through its hydrophobic region and to form, break and isomerize disulfide bonds in these polypep-Keywords endoplasmic reticulum; molecular ... processing step is required for assembly into hexamers [22] A major glycinin, A1aB1b, possess two intramolecular disulfide bonds, Cys12–Cys45 and Cys88–Cys298, which are required for hexamer assembly ... activity produced through the regeneration of the active form of reduced RNaseA Data represent the mean ± SD for four experiments The data for GmPDIS-1, GmP-DIS-2 and GmPDIM are from Wadahama et

Ngày tải lên: 07/03/2014, 05:20

... Therefore, the promoter of a C fusiformis fcp gene has been isolated and tested for its applicability in C fusiformis transformations Prim-ers were designed based on a C fusiformis fcp cDNA sequence (cffcpA-3) ... zeocin-resistant transformants per 107cells were obtained using 1 lg plasmid pPd-ble Trang 3(the transformant numbers represent averages fromthree transformation experiments) Thus, C fusiformis transformation ... databank for Phaeod-actylum tricornutum [7], there is now an urgent demand for genetic tools to analyze the function of diatom genes in vivo Genetic transformation meth-ods are at present available for

Ngày tải lên: 07/03/2014, 21:20

... vectors v1;:::;vn;rform a basis for the null space ofU, and therefore ofA An affine function is a linear function plus a constant. Trang 191 5 53 5 : Reduction to echelon form transformsAand b as ... a basis for range(A) Let v1;:::;vhbe a basis for null(A), and extend this basis (theorem 2.4.1) into a basis v1;:::;vnfor Rn Then we can show that then;hvectorsAvh+1;:::;Avnare a basis for the ... offered for the importance of linear systems The first is apparently deep, and refers to the principle of superposition of effects For instance, in dynamics, superposition of forces states that if force

Ngày tải lên: 14/03/2014, 14:20

... chloroform using liquid-liquid extractions in a separatory funnel The chloroform extract is extracted with acid The acid extract is then neutralized and extracted with chloroform The final chloroform ... concentration for each compound and internal standard Calculate response factors (RF) for each compound using Equation 1 Equation 1 where: A = Response for the parameter to be measured.s A = Response for ... individual falls outside the range for accuracy, the system performance is unacceptable for that parameter Locate and correct the source of the problem and repeat the test for all parameters of interest

Ngày tải lên: 14/03/2014, 20:20

... concentration for each compound and internal standard Calculate response factors (RF) for each compound using Equation 1 Equation 1 where: A = Response for the parameter to be measured.s A = Response for ... individual falls outside the range for accuracy, the system performance is unacceptable for that parameter Locate and correct the source of the problem and repeat the test for all parameters of interest ... laboratory safety are available and have been identified for the information of the analyst.4-6 5 Apparatus and Materials 5.1 Sampling equipment, for discrete or composite sampling 5.1.1 Grab sample

Ngày tải lên: 14/03/2014, 20:20

... examined flocculating properties in an effort to develop an effective extraction method for bioflocculants from the microbial cell surface Materials and Methods Activated sludge samples Activated ... measured for min, and the relative A660 against the control test was calculated every minute using the following formula: The relative A660 = A660 of flocculating test A660 of control test For the ... effective extraction method for activated sludge However, cell disruption is not effective for the isolation of bioflocculants Thus, NaOH extraction was the most effective method for the extraction of...

Ngày tải lên: 05/09/2013, 09:08

... 264 11 Analytical Methods for Monitoring Biodegradation Processes oriented, and not much attention was paid to the identification of environmental requirements for, and testing of, biodegradable ... into low -molecular- weight molecules that are excreted from the body (bioresorption), or dissolving without modification of the molecular weight (bioabsorption) [4, 5] On the other hand, for environmentally ... appear in an oxidized or reduced form after biodegradation depending on whether the conditions are aerobic or anaerobic, respectively 265 266 11 Analytical Methods for Monitoring Biodegradation...

Ngày tải lên: 25/10/2013, 08:20

... Specifications for the Trak-Star Ultrasonic Speed Sensor Page 13 Part I: Sensors for Mobile Robot Positioning Chapter 1: Sensors for Dead Reckoning the kinematic design closely before attempting ... extended axes for all wheels intersect in a common point (adapted from [Byrne et al, 1992]) Page 16 X Part I: Sensors for Mobile Robot Positioning Chapter 1: Sensors for Dead Reckoning For the sake ... while Part II discusses the methods and techniques that use these sensors The report is organized in chapters Part I: Sensors for Mobile Robot Positioning Page Sensors for Dead-reckoning Heading...

Ngày tải lên: 12/12/2013, 21:16

... replacements for mechanical gyros for over three decades With little or no moving parts, such devices are virtually maintenance free and display no gravitational sensitivities, eliminating the need for ... factor linearity that far surpasses the best mechanical gyros Dithered biasing, unfortunately, is too slow for high-performance systems (i.e., flight control), resulting in oscillatory instabilities ... high-tolerance machining and clean-room assembly required for ring-laser gyros The glass fiber in essence forms an internally reflective waveguide for optical energy, along the lines of a small-diameter...

Ngày tải lên: 22/12/2013, 20:16