molecular biology and biological functions of endothelin

... Firstly, software LigandScout [35] was used to analyze molecular interactions of indomethacin and naproxen Fig 4 a Changes in body weight of CFA‑induced RA in rat with compounds 9 and 15 b Effect of ... 166 ppm and the methythio-, methylsulfonyl- and phenoxyl-bearing carbon signals at 156.6, 161.3 and 167.1, respectively 1H NMR of 3 and 5 showed a char-acteristic singlet of methylthio and methylsulfonyl ... reversible, and weak inhibitor binding Contrary to other NSAIDs that inhibit COX reversibly and rapidly (mefenamic acid and ibuprofen), or irreversibly and slowly (indomethacin and diclofenac),...

Ngày tải lên: 29/05/2020, 12:32

... the presence of C–H and C=C group in aromatic ring, respectively and characteristic bands at 3331 and 1604 cm−1 for the presence of –NH2 and N=CH str The structure of the bis-chalcone and its cyclized ... between hydroxyphenyl ring and imidazole ring of His 106, and between chlorophenyl ring and indole ring of Trp105, and π–π stacking between pyrimidinyl ring of compound q5 and Tyr32 phenyl ring is ... studies and elemental analysis The IR spectrum of bis-chalcone (I) showed the characteristic band at 1693  cm−1 which indicated the presence of a –C=O group and characteristic bands at 3088 and 1427 ...

Ngày tải lên: 29/05/2020, 12:32

... of 3.93 Å and has a binding energy of −0.0 kcal/mol The third type of interaction is side chain donor between Glu155 and the bridging sulphur atom of the ligand having bond length of 3.55 Å and ... interac-tion with bond length of 3.78 Å and binding energy of −2.9 (kcal/mol) and hydrogen bond acceptor interaction with bond length of 3.59 Å and binding energy of −1.5 (kcal/mol) with Lys129 ... energy of −0.6 (kcal/mol) with Ile10 There was a hydrogen accep-tor interaction between Gln131 and one of the sulphur atom of the compound with bond length of 4.05 Å and binding energy of −1.1 kcal/mol...

Ngày tải lên: 29/05/2020, 15:04

... and assess morphological, biological, molecular, and pathological characteristics of Pythium cucurbitacearum causing root rot disease of citrus trees to prevent and biological control - To collect ... susceptibility, and environmental conditions, entire fields can be devastated Key symptoms include browning and weakening of root ends, root lesions of varying sizes and colors, root decay, and yellowing of ... center of 90mm petri dishes ( 5 mm diameter holes) on 6 types of mediums and placed at 25°C Six types of mediums were tested: - Potato dextrose agar ( PDA: 250 g of potato, 20 g of agar, 18g of dextrose...

Ngày tải lên: 11/07/2023, 14:16

... Glycobiology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University, Sendai, Miyagi, Japan Introduction Glycosylation is involved in a variety of physiological and ... of glycosyltransferase regulation to the function of the adhesion molecules integrin and E-cadherin. Biological significance of GnT-III, GnT-V and Fut8 GnT-III GnT-III catalyzes the addition of ... the upregulation of GnT-III. In fact, the results of several studies suggest that E-cadherin can induce ligand-independent activation of EGFR and subse- quent activation of Rac1 and MAP kinase,...

Ngày tải lên: 18/02/2014, 17:20

... sequences of Mb and Ngb and the comparison of Fig 2 CD characterization of Ngb and Mb at neutral and acidic pH. (A) Far-UV CD spectra of human holoNgb and apoNgb dissolved in 20 m M Tris ⁄ HCl and ... understanding of the molecular features of Ngb in dictating its biological function is of great interest, especially considering the possible implications of this protein in the pathophysi-ology of ... Ngb and H64Q mutant of Ngb was followed by measuring the decrease in intensity of the Soret band (Ngb, kmax of 412 nm; H64Q, kmax of 406 nm; Mb, kmax of 409 nm) in the absorption spectra of the...

Ngày tải lên: 07/03/2014, 02:20

... and biological consequences of the substitution of Phe7 and Phe8 by (R)-b2-HPhe and of Gly9 by HGly (R)-b2 -HAla or (S)-b3-HAla [(R)-b2-HAla9]SP has pharmacolo-gical potency similar to that of ... screening of 4Ær and no cut-off was used Minimum-energy conformers of b- and a-amino acids were generated by molecular dynamics at high tempera-ture followed by energy minimization Two thousand structures ... intervals of 30 for each torsion angle /, h, and w and were subsequently refined by varying /, w angles in intervals of 10 and setting h angle to) 60 ± 10 and 60 ± 10 Each /, h, and w-value...

Ngày tải lên: 08/03/2014, 08:20

... clarification of juices, improvement in consistency of beer and the digestibility of animal feed stock 5 Application of xylanase in the saccharification of xylan in agrowastes and agrofoods intensifies ... is to discuss the properties and molecular biology of xylanases, genetics of microorganisms producing xylanases and applications Xylan, one of the major components of hemicelluloses found in plant ... terrestrial plants and accounts for 30% of the cell wall material of annual plants, 15-30% of hard woods and 7-10% of soft woods Xylan is a heteropolysaccharide having O-acetyl, arabinosyl and 4-O-methyl-D-glucuronic...

Ngày tải lên: 13/03/2014, 21:56

... Hedman1 and Maria Soderlund-Venermo1 ă Department of Virology, Haartman Institute and Helsinki University Central Hospital, University of Helsinki, Finland Department of Molecular Cell Biology, ... pathogen of the Circoviridae family, and is currently classified as a member of a new, floating genus, Anellovirus Molecular and cell biological research on TTV has been restricted by the lack of permissive ... [19], bone marrow [10] and several other organs [20] However, the main host cells and the target organs of this virus are still undefined The study of the biological functions of TTV is particularly...

Ngày tải lên: 16/03/2014, 05:20

... monomeric form of Tat is biologically active [31] In view of the importance of storage and handling of Tat for preserving its biological functions, we evaluated and optimized... ... macrophages and cells of other lineages and cause cell maturation, upregulation of costimulatory molecules, and release of several potent cytokines, prostaglandins, interleukins and platelet ... were free of contaminants and retained biological functions as evaluated in a range of assays including the induction of cytokines, upregulation of chemokine coreceptor, transactivation of the viral...

Ngày tải lên: 13/08/2014, 09:20

... regulation of mitochondrial function and the impact on 9.2.3 Regulation and function of GFRα and co-receptor isoforms in neurogenesis 1599.2.4 Functions of miRNA in GFL signaling and neurogenesis ... combinatorial interactions of GFLs, GFRα and RET receptor isoforms provides a new paradigm that allows a single ligand to exert a plethora of biological effects Trang 12List of Figures and Tables Figure ... Biochemistry and Molecular Biology (IUBMB) & Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB), Melbourne, Trang 211.6 Conference Presentation 1 Zhou L.H and Too H.P...

Ngày tải lên: 09/09/2015, 10:13

... how the complex functions as a whole, or the degree of cellular protein involvement Till date, only a number of enzymatic functions have been assigned to several nsps and a handful of host proteins ... Trang 1MOLECULAR BIOLOGY AND PATHOGENESIS OF CORONAVIRUS: VIRUS AND HOST FACTORS INVOLVED IN VIRAL RNA TRANSCRIPTION TAN YONG WAH (B SC., NATIONAL UNIVERSITY OF SINGAPORE) A THESIS ... characterization of the interaction between nsp8 and nsp12 revealed that the interaction is independent of the presence of RNA was subsequently shown that nsp8 interacts with both the N- and C-termini of nsp12...

Ngày tải lên: 09/09/2015, 18:54

... deregulation of the cell cycle which results in failure of cellular necessary to develop and synthesize new bioactive mol-ecules whose chemical structure and mode of action are Discovery of drug is ... (48.4 μM) and formation of H-bond with amino acid residues Val27 and Lys153, pi-cation interaction with Arg356 and salt bridge with Asp173, Lys52 and Glu66 within the binding pocket and compound ... class of enzymes that controls the cell cycle and are novel targets for prospective anticancer substituents was developed and screened for CDK1 and 4-cyclohexylmethoxy-pyrimidines (inhibitors of...

Ngày tải lên: 29/05/2020, 13:51

... of fleshy fruit biology and the underlying molecular and biochemical processes that contribute to fruit ripening and the resulting desirable attributes of fruits and fruit products This book offers ... written and when it is read. 1 2013 Trang 5Sonia Osorio and Alisdair R Fernie Sandra Knapp and Amy Litt Ethylene and Climacteric and Nonclimacteric Fruits 46 A Molecular Explanation for System-1 and ... Trang 2The Molecular Biology and Biochemistryof Fruit Ripening Trang 3The Molecular Biology and Biochemistryof Fruit Ripening Edited by GRAHAM B SEYMOUR MERVIN...

Ngày tải lên: 04/10/2023, 15:48

... plausibly involved in DNA recognition and binding and is positioned left of the active site [14] Rep245 is both capable of de novo synthesis of DNA primers and of elongating them Long DNA extension ... Ca atoms of the catalytic triad, are positional homologs of the R148 and K300 residues of P furiosus archaeal primase, both of which are known to play a pivotal role in the activity of this protein ... side-chains of the first pair of residues, i.e K135 and R148, matched almost exactly; those of the second pair were in close proximity The R148 residue of the Pfu-prim-ase is part of a motif which...

Ngày tải lên: 18/02/2014, 18:20

... bonds between NH of Thr5 and CO of Arg2, as well as NH of Arg2 and CO of Thr5 Structure of cyclic hexapeptides – cYT peptide The NMR data of the cYT peptide were indicative of its flexible nature ... intensity of ROE cross-peaks, and were assigned as strong, medium and weak ˚ represent the family Based on ROE violation > 0.2 A and allowed values of /, w in the Ramachandran map, only one family of ... coefficients of chemical shift of amides Glu1 and Thr5 were near 3.8–4.0 p.p.b.ỈK)1 which may be due to intramolecular hydrogen bonding or solvent-shielded amide protons of Glu1 and Thr5 Molecular...

Ngày tải lên: 19/02/2014, 13:20

... Fragments of HSQC and HMBC spectra of compound 1. Labels illustrate assignment of the amide linkage between the amino group of Asp and the carboxyl group of GalA residue E. Scheme 1. The structures of ... out on Sephadex G-50 (2.5 · 95 cm) and Sephadex G -15 columns (1.6 · 80 cm) in pyridinium-acetate buffer, pH 4.5 (4 mL of pyridine and 10 mL of AcOH in 1 L of water), and the eluate was monitored by ... min), dried and analyzed by GC-MS with the standards prepared from D -GalN and (R)- and (S)-2-BuOH. The absolute configuration of L -aspartic acid was deter- mined by chiral HPLC of the oligosaccharide...

Ngày tải lên: 19/02/2014, 16:20

... two isoforms of the protein, only the molecular mass of 50 kDa would suggest that we had purified the truncated isoform Cloning of the cDNA of two isoforms of tomato b-fructofuranosidase and recombinant ... specific 5¢-and 3¢ primers selected on the basis of the published sequences for tomato b-fructofuranosidase (GenBank accession no D11350 and S70040) Primers for the short isoform of b-fructofuranosidase ... werepreincubated with 10 lg of purified glycopeptide and 100 lg of allergen extract before incubation of the blot strips Circular dichroism (CD) spectroscopy of natural and recombinant b-fructofuranosidase...

Ngày tải lên: 21/02/2014, 00:20

... 1. Molecular forms of Scpep1. (A) Analysis of molecular forms of Scpep1: 100 lg of cell lysates (C) and 50 lL of medium (M) of HT1080 and HT1080-Scpep1 were separated by SDS-PAGE, blotted and ... overlap and distinct functions of Scpep1 and Ctsa Purification of Scpep1-His6 from stably expressing HT1080 cells Materials and methods Deglycosylation by PNGase F Cell lines and cell ... the accumulation of undigested materials in the lysosomes. As a result of the clinical relevance of soluble lyso- somal proteins in LSDs and a notable number of LSD-like diseases of unknown etiology,...

Ngày tải lên: 07/03/2014, 03:20

... at 25
C , 30
Cand 35
Cin order to identify scalar and dipolar connectivities among heme and amino acid residues Spectral widths of 13 kHz and mixing times of 80 ms for NOESY and 50 ms for TOCSY ... Tyr66(E7) (G) and Leu70(E11) (E), and to NHs of heme-residue contacts Tyr66(E7) and Phe46(CD1) to have propionates (A, B, C) and interresidue con-tacts from Tyr66(E7) to Phe46(CD1) and NHs of Arg48(CD3) ... Trang 1H NMR study of the molecular structure and magnetic properties Weihong Du1, Zhicheng Xia1, Sylvia Dewilde2, Luc Moens2and Gerd N La Mar1 1 Department of Chemistry, University of California,...

Ngày tải lên: 23/03/2014, 17:22