modelling nanoparticle virus and cell adhesion

... chemokines and cell adhesion molecule in A549 cells cocultured with MT2 cells A549 cells were cocultured with MMC-treated MT-2 or CCRF-CEM cells At and days after cocultivation, A549 cells were ... IL-8 and CCL20 and Induction of cell surface ICAM-1 expression in A549 cells cocultured with MT-2 cells Secretion of IL-8 and CCL20 and Induction of cell surface ICAM-1 expression in A549 cells ... of NF-κB and AP1 A549 cells cocultured with MT-2 cells exhibited high transcriptional activity of NF-κB and AP-1, compared with control A549 cells and A549 cells cocultured with CCRFCEM cells (Figure...

Ngày tải lên: 13/08/2014, 05:21

... for inhibition of the T -cell adhesion mechanism Materials and methods Peptides The linear and cyclic peptides lER, lVY, cER, cVY, cEL and cYT (Table 1) were designed and purchased from Multiple ... buffered saline (NaCl/Pi) and 0.25% (v/v) dimethylsulfoxide Cell suspensions and peptide solutions (80, 200 and 500 lM) were aliquoted into a 48-well cell culture cluster and incubated at 37 °C ... %peptide ¼ (Jurkat cells without formation of E-rosettes/total Jurkat cells) · 100% Lymphocyte-epithelial adhesion assay Caco-2 cells were used between passages 50 and 60 and were plated onto...

Ngày tải lên: 19/02/2014, 13:20

... CL and CLAP, the catalytic and noncatalytic subunits, respectively, of the protease are shown on the right, while protein standards are shown on the left (B) Lanes labelled e (1.5 and 3.0 lg) and ... IEF standards (Std) and buffer control gel run in parallel The numbers at the right represent the pI values of the major bands in e and f, while the numbers at the left are the pI values of standard ... CLAP and BSA, artemin, and p26 to the Artemia CL Results showed that CLAP is resistant to hydrolysis by CL at 30 °C and pH 5.0, while BSA and two abundant proteins in Artemia embryos, artemin and...

Ngày tải lên: 07/03/2014, 16:20

... the central and peripheral nervous systems, different isoforms are found in haematopoietic cells, osteoblasts, vascular endothelial cells and leukocytes, and are induced in various cell lines ... microtitre-plate adhesion assay By using an electrical cell substrate impedance-sensing method, they demonstrated that the level of cell substratum adhesion of neuroblastoma cells correlates ... receptors for extracellular matrix components, thereby providing cells with an avenue for the transfer of information from the extracellular space to the cytoskeleton and intracellular signaling...

Ngày tải lên: 23/03/2014, 07:20

... b-catenin can influence cell adhesion and apoptosis [23,28] DDR1 is an integrin-independent cell adhesion molecule DPIV reduces cell adhesion by dephosphorylating p38 MAP kinase and b1-integrin [29], ... further established these roles in cell ECM interactions [12] Cell adhesion is crucial in monolayer wound healing and cell migration Therefore, the adhesion defect of cells overexpressing DP8 or DP9 ... decreased cell adhesion In vitro cell adhesion of cells transfected with dipeptidyl peptidase (DP8)–GFP, DP9–GFP and GFP control is expressed as a ratio of the percentage of fluorescent cells in...

Ngày tải lên: 23/03/2014, 11:20

... Minimal model of b -cell mitochondrial Ca2+ handling Am J Physiol 273 (Cell Physiol 42), C717–C733 164 Magnus, G & Keizer, J (1998) Model of b -cell mitochondrial calcium handling and electrical activity ... the cells is necessary for the maintenance of oscillations Removal of external Ca2+ leads to a cessation of oscillations in most cases in endodermal cells [62] and HeLa cells [63] In other cell ... [197] Intercellular entrainment of Ca2+ oscillations has also been observed in other cell types, such as pancreatic acinar cells [198], articular chondrocytes [199,200], kidney cells [201], and in...

Ngày tải lên: 24/03/2014, 00:21

... activity, and thereby stimulate cell spreading and motility in neuroblastoma cells [34] It is possible that different small G proteins are involved in LPA-induced cell motility in different cell types ... Trends Cell Biol 8, 151–157 17 Gilmore, A.P & Romer, L.H (1996) Inhibition of focal adhesion kinase (FAK) signaling in focal adhesions decreased cell motility and proliferation Mol Biol Cell 7, ... actin stress fibers and focal adhesion [31] LPA1 and LPA2 receptors couple to MAPK/ ERK, PLC and RhoA-dependent signaling cascade, while LPA3 receptors activate only MAPK/ERK and PLC pathways [32]...

Ngày tải lên: 30/03/2014, 13:20

... syngeneic Ag-specific SV40-immortalized target cells, and either expanded CTLs or fresh peripheral blood mononuclear cells (PBMCs) as effector cells Methods Cells Specific-pathogen-free primary chick ... level of CD8+ T-cells and a strong T-helper [21] response, so that a poor clinical efficacy is often obtained [21] Vaccinia virus (VV) recombinants expressing the HPV16 and HPV-18 E6 and E7 proteins ... integrated HPV-16 DNA, and green monkey kidney (Vero) cells were grown in DMEM supplemented with 10% CS and P/S Rabbit PBMCs were obtained from heparinised rabbit blood and used for CTLs and cytokine assays;...

Ngày tải lên: 18/06/2014, 16:20

... infectious virus production and virus- induced cell fusion The results suggest that virus- induced cell fusion mechanisms are not required for cytoplasmic virion envelopment Materials and methods Cells and ... for virus egress and cell- to -cell fusion [28,29] Recently, we delineated via site-directed mutagenesis the functional domains of UL20p involved in infectious virus production and virus- induced cell ... in intracellular virion envelopment and infectious virus production, while the UL20p domain spanning amino acids 204–211is important for both intracellular transport and virus- induced cell fusion...

Ngày tải lên: 18/06/2014, 18:20

... lumbar, and sacral bones), skull (maxilla, mandible, and cranium), thorax (sternum, ribs, and scapula), pelvis (ilium, ischium, and pubis), foreleg (humerus and radius) and hindleg (tibia and femur) ... treat B16M or A375M cells Cancer Cell Adhesion Assay to Primary Cultured BMSCs Murine and human BMSCs were cultured for 15 days prior to be used in adhesion assays B16M and A375M cells were labeled ... Cells COX-2 BMSC and Melanoma Cell Secretion of VEGF BM Stromal Cell Expression of VCAM-1 VEGF Receptor-Expressing Melanoma Cells COX-2 VLA-4Dependent Melanoma Cell Adhesion Melanoma Cell Proliferation...

Ngày tải lên: 18/06/2014, 22:20

... N-glycan mutants are transported to the cell surface and remain mannose-rich Materials and methods N-glycans play not only an important role in folding and intracellular transport of viral glycoproteins ... tested by peptide standard ELISA [18] and used for immunoblot analyses Mutation and vectorial expression of recombinant Lassa virus glycoprotein The full length glycoprotein of Lassa virus (strain ... mutants Cell surface transport of GP-C N-glycosylation mutants Vero cells were transfected with GP-1 (A) and GP-2 (B) N-glycosylation mutants of Lassa virus glycoprotein At 24 h post-transfection, cells...

Ngày tải lên: 20/06/2014, 01:20

... Figure virus- specific T -cell response Correlation between the Epstein-Barr virus viral load and Epstein-Barr virus- specific T -cell response Correlation between the number of IFNγproducing T cells and ... IFNγ-producing T cells and disease activity (Dis- Figure Epstein-Barr virus- specific IFNγ-producing T cells Number of IFNγ-producing T cells per 106 peripheral blood mononuclear cells (PBMCs) (a) After cells ... Unadapted Epstein-Barr virus- specific T -cell IFNγ production under treatment Five patients demonstrated inappropriate EBV-specific T -cell IFNγ production (1,000 EBV copies...

Ngày tải lên: 09/08/2014, 14:21

... kinase/cSrc, and the small GTPases Ras and Rho) and adaptors (for example, Cas/Crk and paxillin) that assemble within dynamic adhesion structures, including focal adhesions that bind cells to the extracellular ... certain cell types or tissues: αIIbβ3 to platelets; α6β4 to keratinocytes; αEβ7 to T cells, dendritic cells and mast cells in mucosal tissues; α4β1 to leukocytes; α4β7 to a subset of memory T cells; ... ligand-binding pocket of the headpiece that increase its affinity for ligand The affinity directly regulates the nature of the ligand binding and appears to tune the degree and kinetics of cell...

Ngày tải lên: 14/08/2014, 07:21

... at cell- adhesions As cell adhesions play a pivotal role in the proper function of cells, misregulation of cell adhesions often leads to severe pathological consequences Altered cell- cell and cell- matrix ... conformation changes At subcellular level, vinculin plays important roles in regulating cell- matrix and cell- cell adhesions Cells lacking vinculin develop less stable focal adhesions and migrate faster ... transduction across cells, cellular recognition and triggering of immune responses Tissue organizations not only depend on cell- cell adhesions In epithelium and muscles, cells are surrounded...

Ngày tải lên: 09/09/2015, 08:13

... components of the nuclear lamina and sarcomeres They also participate in cell- cell and cell- matrix adhesive junctions 2.2 Basics of Cell Adhesion In biological systems, cell adhesion is an integrated ... migration, and cell- cell and cell- ECM adhesions In the case of adhesion, the lateral mobility of receptors determines the speed at which receptors move towards their corresponding ligands, the ... forces used in cellular contraction and basic cell movements They also enable a dividing cell to pinch off into two 11 cells and are involved in amoeboid movements of certain types of cells The final...

Ngày tải lên: 10/09/2015, 15:48

... in cell- cell adhesion to ensure adequate communication and also the binding between cells and extracellular matrix (ECM) proteins Furthermore, CAMs are known to trigger intracellular events and ... Standard gravity as unit for acceleration Introduction 1 Introduction 1.1 Cell adhesion molecules Cell adhesion is crucial for the development and maintenance of tissue structures and multicellular ... fucosylated and sialylated glycoprotein ligands on other cells and mediates adhesion of leucocytes and platelets to vascular surfaces Thereby, selectins are involved in constitutive lymphocyte homing, and...

Ngày tải lên: 25/11/2015, 13:27

... epithelial tissues Mol Cell Biol 24:7538–7547 Senescence and Cell Cycle Control 269 Munger K, Howley PM (2002) Human papillomavirus immortalization and transformation functions Virus Res 89:213–228 ... suppression and cell mortality Cell 85:27–37 Serrano M, Lin AW, McCurrach ME, Beach D, Lowe SW (1997) Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a Cell ... in cultured mouse cells p19ARF is predominantly upregulated during senescence by oncogene activation and/ or oxidative stress Senescence and Cell Cycle Control 265 In human cells undergoing replicative...

Ngày tải lên: 25/10/2013, 21:20

... the daughter cells at the end of cell division and in cellular autolysis [9], where it mediates the release of toxins that damage the host tissues and allows the entry of pneumococcal cells into ... analysis of ligand binding to C-LytA (A) Conformational changes induced by ligands on C-LytA monitored by near-UV CD with no ligand added (—) and upon addition of ligands: s, d, 2.5 and 20 mM choline; ... analogs on cell growth and viability Fig Effect of choline and analogs on the activity of cell wall lytic enzymes Data are shown as percentage of activity with respect to nonligated enzyme, and are...

Ngày tải lên: 19/02/2014, 05:20