miniaturization technologies for high throughput biology

... developed high-throughput mRNA sequencing (eoRNA-Seq) is another high thioughput method to ienbfy and quan0fy transeript isoforms [10-11] However, is high processing cost, low sample throughput, ... sample throughputs are sil ae tobe improved more puicularly For a age clini! setting The developmen of analytical methods is another essential pat in traslaional medicine, in particular, for high-throughput ... ments in knowledge-based analysis for high-throughput genomie data In Chapter 5 ove present a typical translational study for severe traumatic patients, where high Uhzoughput gesomie technology

Ngày tải lên: 21/02/2014, 05:20

... TECHNIQUES FOR HIGHTHROUGHPUT IDENTIFICATION OF PROTEINS HU YI (B.Sc.) NATIONAL UNIVERSITY OF SINGAPORE 2006 DEVELOPMENT AND APPLICATION OF ADVANCED PROTEOMIC TECHNIQUES FOR HIGHTHROUGHPUT ... from three different aspects for high-throughput identification of enzymes and their associated proteins in yeast proteome (catalomics). Firstly, to validate the high-throughput capacity of differential ... techniques for highthroughput protein identification and characterization (Aebersold and Mann, 2003; Hu et al., 2004) Among these techniques, 2-DE is a routine tool for large-scale

Ngày tải lên: 16/09/2015, 08:30

... Maramis et al BMC Bioinformatics (2018) 19:144 https://doi.org/10.1186/s12859-018-2144-z SOFTWARE Open Access IRProfiler – a software toolbox for high throughput immune receptor profiling ... software pipeline for immune repertoire profiling of high-throughput TR and BcR sequencing data, called Immunogenetic Repertoire Profiler or IRProfiler IRProfiler covers two of the aforementioned ... established bioinformatics platform with a large community of users [16] This allows IRProfiler to benefit from the straightforward, easy-to-use interface of the Galaxy platform  Ease of access

Ngày tải lên: 25/11/2020, 15:39

... trade accuracy for speed This trade-off is undesirable for most applications, and it is particularly problematic for robotics and CAD Additional research is necessary to find sufficient throughput ... microarchitectures for performing broad-phase collision detection that offer significant throughput and power advantages relative to CPU and GPU equivalents • A novel technique for combining these ... before complementing this with a description of the spatial-partitioning broad phase 3.1 Cell-based broad phase We chose a microarchitecture as the platform for our cell-based broad phase for

Ngày tải lên: 08/11/2022, 14:56

... effects requires high-throughput cellular analysis meth-ods that are not currently available These examples highlight instances in which measures of bulk cell response are uninformative with respect ... cell imaging analysis on a platform that is amenable to automation and high throughput applications and will be readily usable by cell biologists This approach, therefore, overcomes several limitations ... Trang 1A method for high-throughput functional imaging of single cells within heterogeneous cell preparations Adam S

Ngày tải lên: 19/11/2022, 11:42

... screened for the desired characteristics [23–25] However, the application of this technique for developing effective lignin-degrad-ing enzymes is limited due to the lack of an efficient high-throughput ... improvement of lignin-degrading enzymes by high-throughput selection techniques such as directed evolution is currently limited, as robust methods for detecting the conversion of lignin to desired ... Trang 1Development of a genetically programed vanillin-sensing bacterium for high-throughput screening of lignin-degrading enzyme libraries Barindra Sana1, Kuan Hui Burton

Ngày tải lên: 24/11/2022, 17:55

... Semi-automated platform High content for higher statistical precision Suitable for dose response and mode of action analysis for most substances Images of nuclei and MN can be stored for re-validation ... therefore be used for dose response analysis as substantially higher number of cells can be scored with these methods which allows for much statistical power A test system that combines the high high-throughput, ... the images Flow cytometry MicroFlow ® Fully automated platform to score MN objectively Suitable for dose response High content and high throughput Permits cell cycle analysis 10,000 events scored

Ngày tải lên: 24/11/2022, 17:56

... and cross-reactivity against other subtype was 0% (0/6) for both platforms Conclusion MAGPIX and DPP platforms can be utilized for high-throughput and in-field detection of novel influenza virus ... serum adsorption was performed and the highest fold rise in MFI and DPP values was used in analysis, assay sensitivity for H5 was 100% for MAGPLEX and 83% for DPP; specificity for H5 was 100% and ... multiplex platforms for antibody detection The multiplexed magnetic fluorescence microsphere immunoassay (MAGPIX) is a high throughput laboratory-based assay Chembio Dual Path Platform (DPP) is

Ngày tải lên: 04/12/2022, 16:01

... et al Genome Biology 2010, 11:R39 http://genomebiology.com/2010/11/4/R39 Open Access METHOD miRTRAP, a computational method for the systematic identification of miRNAs from high throughput sequencing ... for the discovery of novel miRNA genes A computational approach called miRTRAP (miRNA Tests for Read Analysis and Prediction) was developed for the systematic prediction of miRNAs from high-throughput ... miR Our approach for the identification of microRNAs using high-throughput sequencing reads is to compute a set of quantities for each candidate locus, and by using thresholds for each quantity

Ngày tải lên: 09/08/2014, 20:21

... 5'-TTGCAAGTTTGCTGCTGAAC-3' (95°C for 10 minutes followed by 40 to 50 cycles of 94°C for 15 s, 59°C for 20 s, 72°C for 30 s and 76°C for 5 s (sig- nals acquired)), whereas the primers for the promoter of mir-21 ... were treated with 10 μM doxorubicin (Sigma Dorset, UK) for 2 h before being allowed to grow for another 24 h, 200 μM deferroxamine (DFX; Sigma) for 24 h, with or without 1 μM NF-κB acti- vation inhibitor ... 5'-TACAAACTGGGGAGCTTGGT-3' and 5'-AACCCCTGCGTCATCCTTAT-3' (95°C for 10 min- utes followed by 40 to 50 cycles of 94°C for 15 s, 59°C for 20 s and 72°C for 30 s (signals acquired)). PCR signals were standardized

Ngày tải lên: 11/08/2014, 12:20

... 1SYSTEM-LEVEL MODELING OF ENDOTHELIAL PERMEABILITY PATHWAY AND HIGH-THROUGHPUT DATA ANALYSIS FOR DISEASE BIOMARKER IN COMPUTATION AND SYSTEMS BIOLOGY (CSB) SINGAPORE-MIT ALLIANCE NATIONAL UNIVERSITY ... genes in breast cancer samples and normal samples (Red for higher relative enrichment level, blue for lower relative enrichment level and white for medium enrichment level) 171    Trang 14Table ... formats like systems biology mark-up language (SBML), a standard for representing models of biochemical and gene-regulatory networks [131, 132] 1.3 Introduction to high-throughput biomarker selection

Ngày tải lên: 09/09/2015, 10:14

... APPLICATION OF A HIGHTHROUGHPUT SCREENING ASSAY FOR DISCOVERY OF STARCH HYDROLASE INHIBITORS LIU TING TING NATIONAL UNIVERSITY OF SINGAPORE 2013 DEVELOPMENT AND APPLICATION OF A HIGHTHROUGHPUT ... . - 19 2.4 Functional food for diabetes patients - 21 Chapter 3. A high-throughput assay for quantification of starch hydrolase inhibition based ... consumption of low glycemic index (GI) foods. To search for the active ingredients in low GI foods, a 96well based high-throughput method (HTS) for rapidly measuring the inhibition of starch hydrolase

Ngày tải lên: 10/09/2015, 09:01

... conclusion, DNA chip platform is a high-throughput and reliable technology forgenetic diagnosis and pharmacogenetic profiling Trang 11LIST OF TABLESPage Table 2.1 PCR primers for the amplification ... Minisequencing/denaturing high-performance liquid chromatography (DHPLC)In 2003, Wu, et al., (2003) reported the application of using the minisequencing followed by denaturing high-performance liquid chromatography for ... liquid-phase assay formats, a feasible solution is todesign the minisequencing assay on the solid phase, for example, on the DNA chipplatform Solid-phase assay formats In solid-phase assay formats, oligonucleotide

Ngày tải lên: 12/09/2015, 08:18

... method used for the preparation of fortified samples remained the same as unfortified samples The fortification (spiking) of samples was done before the final dilution stage The fortified samples ... and high-throughput strategy for natural product dereplication in five Salvia species using high-resolution ESI-QTOF-MS/MS data In the present study, we were able to identify a total of forty-seven ... analytes including their class, formula, molecular weight and the instrument polarity used for analysis are listed in Table S1 Formic acid was used as an additive for the mobile phase and purchased

Ngày tải lên: 11/05/2020, 10:29

... 1R E S E A R C H A R T I C L E Open AccessIdentification of candidate genes for drought tolerance in coffee by high-throughput sequencing in the shoot apex of different Coffea arabica cultivars ... to isolate the shoot apex (Fig 1b) For microscopic analyses, leaves identical to those used for plants At the end of the 2011 dry season,Ψpdwere mea-sured once for Rubi and IAPAR59 plants under ... collected (Fig 1a) for gene expression analyses (qPCR) RNA isolation, DNA synthesis and 454-sequencing The plagiotropic buds were incubated for 5 min in the washing buffer (66 % chloroform, 33 % methanol,

Ngày tải lên: 22/05/2020, 04:18

... to be excellent tools for these research objectives, to date there is no SNP database or SNP arrays available for WWP Next generation sequencing (NGS) strategies for high-throughput SNP discovery ... DNA markers for breeding and conservation programs of this important conifer species The Sequenom iPlex has been reported as one of highly reliable high-throughput SNP genotyping platforms with ... (95°C for 20 sec, 56°C for 30 sec, 72°C for 60 sec), and final extension at 72°C for The shrimp alcaline phosphatase was used to remove all unincorporated dNTPs After single base extension for

Ngày tải lên: 26/05/2020, 23:26

... obtaining knowledge in teaching maths for students, especially at high school level, has not been deeply studied In fact, there are not any studies of teaching maths for high school students oriented ... activities in teaching maths for high school students; 2.2 Find out the reality and need of improving the competency of exploring and obtaining knowledge in teaching maths for high schools students ... chemistry and biology; 2.3 Build and test the feasibility of pedagogy method applied in teaching maths at high school classes majoring in physics, chemistry, and biology to foster this capacity for students

Ngày tải lên: 07/06/2021, 17:14

... low storage density, and have miniaturization challenges These systems are not suitable for high delta-V maneuvers for the same reasons that other monopropellant technologies fall short, but they ... constant of sublimation for trials conducted at 20 ̊C for four different sample holder diameters 54 Figure 2.14: Time constant of sublimation for trials conducted at 30 ̊C for four different sample ... constant of sublimation for trials conducted at 40 ̊C for four different sample holder diameters 55 Figure 2.16: Time constant of sublimation for trials conducted at 50 ̊C for four different sample

Ngày tải lên: 24/10/2022, 01:51

... blocks for metabolomic analysis, which in turn limits the subsequent bioinformatic analysis and construction of biologically informative models [16,21] Currently, NMR is of limited use for high throughput ... minutes and provides information on their rela-tive levels in the sample [27-29], making it very useful for high throughput metabolome analysis However, it lacks specific information as to the identity ... identify high throughput metabolomic data [12,13] Cumulatively, the unusual metabolic networks of organisms in ecological niches are renewing interests in metabolites that high-light the lack of high

Ngày tải lên: 02/11/2022, 14:24

... Trang 1A high-throughput microfluidic approach for 1000-fold leukocyte reduction of platelet-rich plasma Hui Xia, Briony C Strachan, Sean C Gifford† & Sergey S Shevkoplyas ... to 80.8% for RS-7, from 90.2% to 86.0% for RS-8, and from 91.5% to 88.6% for RS-9 as the driving pressure increased from 6.25 PSI to 25 PSI (Fig. 4c) In contrast, the platelet recovery for the ... expression) for platelets recovered from the centre (retentate) and side (fil-trate) outlets of the devices The MPV values for platelets from the retentate were lower (and from the filtrate – higher) for

Ngày tải lên: 08/11/2022, 14:56