microrna expression in disease

... with our findings MicroRNA expression was correlated with their target gene expression in pollen Since we were able to detect the expression of RNA silenc-ing pathway genes and some miRNAs in mature ... types The expression of the remaining miRNAs was detected in pollen and inflorescence Among them, 7 had Expression of RNA silencing pathway genes in Arabidopsis mature pollen Figure 1 Expression ... used to inspect the expression of all members of the DCL, AGO, RDR and Double-stranded RNA binding protein (DRB) genes (Figure 1) The results indicated that most of the genes in RNA silencing pathways

Ngày tải lên: 12/08/2014, 03:20

... the microRNAs [33-38] Determining spatial and temporal patterns of microRNA expression should yield insight into the biological functions of microRNAs As the number of microRNAs identified has increased ... indicate that murine brain development involves a wave of expression of sequential classes of microRNAs (Figure 3a) We also grouped the developmental time points according to their microRNA expression ... a free amino group linked to its 5' terminus through a 6-carbon spacer (IDT) and was printed onto amine-binding slides (CodeLink, Amer-sham Biosciences) Control probes contained two internal

Ngày tải lên: 14/08/2014, 14:21

... in regulating brain development early in life and mediating synaptic plas-ticity later in life, we have hypothesized that the etiopathology of schizophrenia might be associated with altered expression ... proteins (hnRNPs) [30], known to chaperone other RNA events The bioinformatic similarities involving motifs, though not yet investigated in vivo, are consistent with the hypothesis that the coordinated ... Nuclear membrane Motif binding Trang 7etiopathology of schizophrenia, and whether the above motifs are involved in regulating miRNA processing from pri-miRNA to pre-pri-miRNA As a final note, DiGeorge

Ngày tải lên: 14/08/2014, 18:20

... long non-protein-coding RNAs involved in post-transcriptional regulation of gene expression [1, 2] Mature miRNAs join the RNA-induced silencing complex (RISC) in the cytoplasm and bind to messenger ... heterogeneous within an FFPE tumor block Formalin fixation and paraffin embedding caused a decline in miRNA expression of between 0.5 and ~1.5 Cq; yet, correlation between miRNA expression in fro-zen- ... was transferred to a container and kept in the freezer at -80 ° C The remaining tumor sections were fixed in formalin for 2, 3, or 6 days and then embedded in paraffin and kept at room temperature

Ngày tải lên: 21/09/2020, 10:21

... and increased expression to increased patient survival [41] Interestingly, eIF6 expression was unaltered in EC cells This indicates the complexity of miRNA biosynthesis regulatory mechanisms in ... S, Calin GA, Volinia S, Liu CG, Scarpa A, Croce CM: MicroRNA expression abnormalities in pancreatic endocrine and acinar tumours are associated with distinctive pathologic features and clinical ... Cancer Institute, Boston, MA02115, USA, 5NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore 117456, Singapore and 6Applied Biosystems, 850 Lincoln

Ngày tải lên: 20/06/2014, 07:20

... while the inhibition of the miRNA increased survival rates Again, since radiation was clearly found to up-regulate miR-20a, this microRNA is another potential candidate in our system linking functional ... overexpression, suggesting that miRNAs may func-tion as tumor suppressors or as oncogenes The involve-ment of miRNAs in tumorigenesis is not the only topic of investigation In addition the expression ... 2such as in the gut microvasculature [10] In contrast, ECare also stimulated by radiation-induced indirect pro-angiogenic factor production including VEGF and bFGF [9,11] Further ionizing radiation

Ngày tải lên: 09/08/2014, 08:23

... knockdown of HO-1 expression by using specific small interfering RNA resulted in upregula- tion of synthesis of proinflammatory cytokines, including inter- leukin-6, interleukin-8 and TNF, which ... Mann-Whitney U-test), indicating that HO-1 expression was reduced in individual cells from patients with active disease. As shown in Figure 1b, HO-1 mRNA lev- Figure 1 HO-1 mRNA expression in PBMCs and ... HO-1 knockout mice and observed in a patient with HO-1 deficiency [22,23]. In RA, HO-1-expressing cells were located in the lining and sublining layers, but not in the carti- lage-pannus junction,

Ngày tải lên: 09/08/2014, 10:22

... reproduction in any medium, provided the original work is properly cited. microRNA expression in retinitis pigmentosa <p>MicroRNA expression profiling showed that the retina of mice carrying a ... evidence of modified miR expression profiles in reti-nal disease Results MicroRNA expression profile in wild-type retina Retinal miR expression was initially evaluated using micro-array analyses ... proteins that are involved in intra-cellular trafficking and motility (for example, Ktn1 [Kinectin 1]), Actr10 [ARP10 actin related protein 10 homolog], and Myh9 [non-muscle myosin heavy chain

Ngày tải lên: 14/08/2014, 08:20

... functions and in disease-inducing pathways has been increasingly demonstrated Owing to the difficulty in obtaining human PD brain tissues and the variations in the time of obtaining the PD tissues, ... 1999) Iron accumulation in the brain of PD patients has been reported and combined with the down-regulation of ferritin (the protein involved in binding to iron to maintain its non-reactive state) ... miRISC microRNA containing rna-induced silencing complex MPER mammalian protein extraction reagent MPP methyl phenyl pyridinium PBS phosphate buffered saline PCR polymerase chain reaction PD Parkinson’s

Ngày tải lên: 10/09/2015, 09:12

... (19°08’17N, 110°15’46E). After acclimatized in our laboratory for 1 week, 18 of 52 eels with similar size and weight were exposed to different salinities for 15 days, including 6 individuals in fresh water (FW, 0‰ salinity), 6 in brackish water (BW, 10‰ salinity) and 6 in sea water (SW, 25‰ salinity). Each individual was dissected on ice and its gill tissues were immediately frozen in liquid nitrogen ... Institution (PAPD), and the Innovation of Graduate Student Training Project of Jiangsu Province (CXLX13­381) Competing interests: The authors have declared that no competing interests exist Introduction ... Osmoregulatory MicroRNA Expression Patterns in Catadromous Eel Anguilla marmorata Xiaolu Wang  , Danqing Yin  , Peng Li, Shaowu Yin  , Li Wang, Yihe Jia, Xinhua Shu Trang 2because maintaining water and ion homeostasis in their gills is indispensable to osmotic adjustment during migration. Hundreds of

Ngày tải lên: 26/09/2019, 16:15

... (Bromodomain-Containing Protein 4), a member of the Bromodomain and Extra Terminal domain (BET) family, as a gene that distinguished T-bet+/high and T-bet−/low tumors In clinical studies, BET inhibitors ... been shown to suppress inflammation in various cancers, suggesting a potential link between BRD4 and immune infiltration in cancer Hence, we examined the BRD4 expression and clinicopathological features ... utilizing intrinsic tumoral characteristics as the sole determining factors of disease progression An increasing number of studies have demonstrated that the components of tumor microenvironment, including

Ngày tải lên: 03/07/2020, 01:13

... saline (PBS) and incubated in 1–2% bovine serum albumin Afterwards, sections were incubated overnight at 4°C in primary antibodies diluted in 1–2% bovine serum albumin The following primary antibodies ... under cold running water, stained with haematoxylin and eosin, washed again under cold running water and dehydrated in alcohol and in xylene Coverslips on slides were fixed using Mounting Medium ... immunohistochemical examination of cytokeratin, vimentin, smooth muscle actin, s100 protein and p63 protein expression For immunohistochemical analysis, tumour samples were embedded in paraffin 3-μm-thick

Ngày tải lên: 06/08/2020, 03:05

... specifically in-volved in the progression of liver disease are not fully characterized Therefore, to better define and identify microRNAs playing a pivotal role in this process, we ana-lyzed in a time-dependent ... made to minimize suffering Assessment of microscopic hepatic lesions Specimens obtained from livers were washed in PBS and immediately immersed in 10 % formalin in phosphate buffered saline (PBS) ... miR-200c binding sites are predicted on Mus musculus ZEB1, ZEB2 and NCAM1 3′UTR, indi-cating its putative role in the mouse model here presented MiR-27a showed expression decrease, starting faintly

Ngày tải lên: 21/09/2020, 10:16

... This is of peculiar interest for cancerogenesis, since miR-93 has been found involved in the down regulation of integrin β-8 [34] and VEGF expression [35] Besides the finding that IL-8 can be ... levels in the same histological areas of the glioma Staining of IL-8 mRNA was observed in similar areas of the glioma that express VEGF Interestingly, the IL-8 and VEGF staining was found mainly ... miR-93 reported in Fig 2c indicates an up-regulation in LGG and HGG in comparison to the expression measured in reference healthy brain Also in the case of miR-93, its levels of expression are

Ngày tải lên: 28/09/2020, 01:42

... confidence interval. Trang 9times were quite similar to those in HR- tumors withoutAR expression Interestingly, HER2 expression seems to interact with AR in HR negative tumors in prognostic terms, as in ... decided to score each apparent staining as positive and opposed it to totally lacking staining No GCDFP-15 staining was seen in non-epithelial cells such as stromal or inflammatory cells Association ... Immunohistochemical expression pattern of GCDFP-15 in breast cancer A Tumor cells of an invasive lobular carcinoma, arranged in indian file pattern and exhibiting moderate cytoplasmic staining for GDCFP-15

Ngày tải lên: 14/10/2020, 13:40

... AccessThe expression of microRNA-375 in plasma and tissue is matched in human colorectal cancer Lingling Xu1, Minzhe Li2, Min Wang1, Dong Yan1, Guosheng Feng2*and Guangyu An1* Abstract Background: MicroRNAs ... tools in CRC MicroRNAs (miRNAs) are small, non-coding single-strand RNAs, 18–25 nucleotides in length They are en-dogenously expressed and post-transcriptionally regulate gene expression by binding ... circulating miR-NAs as stable, minimally invasive biomarkers Taqman microRNA Array was used for miRNA profiling and identified a panel of circulating miRNAs which could be minimally invasive

Ngày tải lên: 14/10/2020, 16:11

... Correspondence: patricia.severino@einstein.br 1 Albert Einstein Research and Education Institute, Hospital Israelita Albert Einstein, Sao Paulo, Brazil Full list of author information is available ... microRNA expression patterns in human malignancies; measurement of microRNA expression may have diagnostic and prognostic applications Roles for microRNAs in head and neck squamous cell carcinomas ... and Trang 2MicroRNAs (miRNAs) are ~22 nt non-coding RNA molecules that negatively regulate gene expression by degrading or destabilizing the messenger RNA (mRNA) or by inhibiting protein translation

Ngày tải lên: 05/11/2020, 05:32

... important to keep in mind that many small, but coordinated, changes in expression can be sig-nificant when considered in combination, even if the changes in expression of the individual miRNAs ... have increased since the discovery that miRNA expression can be measured not only in tissues but also in serum, plasma and urine [5-9] The possibility to measure the expression of miRNAs in body ... multiple testing, using the Benjamini-Hochberg method Hierarchical clustering was performed on all samples and miRNAs investigated using the PermutMatrix clus-tering tool [24], using Euclidean

Ngày tải lên: 05/11/2020, 05:54

... comparing sequences between two individuals (in this case, donor and recipient), or representing diver-sity within one individual at a given time point (in this case, providing a snapshot of within-host ... CRF01_AE clade Known escape mutations in B clade infection are indicated in bold. Trang 6responses, eight of the remaining 12 were in or flanking known epitopes, in seven cases either restricted ... a central role in immune containment in subjects expressing these alleles [8 26–28] The presence in the AE clade consensus of the very residues that are selected in B or C clade infected subjects

Ngày tải lên: 01/11/2022, 09:52

... time, and the remaining genes, including Sphk1 and Birc5 encoding for inhibitory factors, were each downregulated showing a con-sistent temporal decrease in expression levels Clustering of biological ... preprotachykinin (Tac1) Tach-ykinin is involved not only in inflammatory responses, but in neural stimulation as well, thereby bridging inflammation to muscle contraction [26] Phase-specific gene expressions ... autoimmunexocrinopathy; BIRCH: balanced iterative reducing and clustering using hierarchies; EGF: epidermal growth factor; Idd: insulin-dependent diabetes; IL: interleukin; LIMMA: Linear Models

Ngày tải lên: 01/11/2022, 09:56