methods in adipose stem cell engineering

... AccessBusulfan systemic exposure and its relationship with efficacy and safety in hematopoietic stem cell transplantation in children: a meta-analysis Xinying Feng1,3, Yunjiao Wu1,3, Jingru Zhang1, ... when applying the results in Asian location Finally, the use of observa-tional studies in the meta-analysis implies biases and confounding factors, given that these are inherent in the original studies ... 1500 μM × min comparison with ≥1500 μM × min, RR < 1 favors ≥1500 μM × min) Trang 10Competing interestsThe authors declare that they have no competing interests. Author details 1 Clinical Research

Ngày tải lên: 29/05/2020, 18:56

... differences in their immune profiles. The promising role of mesenchymal stem cells (MSCs), whose mechanism of action is predominantly paracrine, in cell-based therapies and tissue engineering appears ... system shows a functional decline in ability to respond to new pathogens during ageing, whereas serum levels of inflammatory cytokines are increased with age34 Inflammaging is a new term coined ... cytokine signalling pathways Therefore, we carried out a functional study of miRNA expression involved in inflammation mediated by chemokine and cytokine signalling pathways in infant, young and

Ngày tải lên: 24/11/2022, 17:48

... M poling • Department of Pediatric General and Thoracic Surgery, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, USA adrian ranga • Laboratory of Stem Cell Bioengineering, Institute ... of 3D-retina from hESCs Trang 39 2 Digest cells at 37 °C for 30 min by using papain (Neural Tissue Dissociation Kit) and gently dissociate into single cells by pipetting 3 Plate single cells on ... Brn3b+ cells (~d28), Crx+ cells (~d35), Recoverin+ (~d45), RXRG+ cells (~d60), NRL+ cells (~d100), S-opsin+ cells (~d130), and Rhodopsin+ cells (~d130) When we applied the “induction-reversal” methods,

Ngày tải lên: 13/05/2017, 21:23

... stem cells into insulin-secreting cells In Vitro Cell Dev Biol Anim 2011, 47:54 –63. 45 Martin I, Jakob M, Schafer D, Dick W, Spagnoli G, Heberer M: Quantitative analysis of gene expression in ... injury was induced by joint destruction in the hind limbs of NOD/SCID mice using a 32 G needle Briefly, 12 mice were anesthetized using ketamine (40 mg/kg) and then subjected to hind-limb joint ... mesenchymal stem cells of fetal (Wharton ’s jelly) and adult (adipose tissue) origin during prolonged in vitro expansion: considerations for cytotherapy Stem Cells Int 2013, 2013:246134. 19 Harn HJ, Lin

Ngày tải lên: 12/12/2017, 05:46

... mouse with acute hindlimb ischemia Keywords: Ischemia, Hindlimb Ischemia, Adipose stem cells, Angiogenesis, Stem cell therapy INTRODUCTION Adipose-derived mesenchymal stem cells (ADSCs) are ... www.bmrat.org 770 Human adipose-derived mesenchymal stem cell in angiogenesis Human adipose-derived mesenchymal stem cell could participate in angiogenesis in a mouse model of acute hindlimb ischemia ... differentiate into endothelial cells, when cultured in a medium containing VEGF to take part in angiogenesis They contribute in new blood vessel formation in hindlimb ischemia mouse models by stimulating

Ngày tải lên: 16/12/2017, 18:01

... source of stem cells for cartilage tissue engineering Keywords: Alginate/hyaluronic acid hydrogel, Chondrogenic differentiation, Cartilage tissue engineering, Mesenchymal stromal/stem cells, Wharton’s ... spraying system consisted of an airbrush working with a com-pressor to induce spraying, with pressure being equal to 0.9 bar The spraying bottle containing the homoge-nized cellular suspension in ... eliminated in the last step of necro-sis This could explain the increase in sprayed cell via-bility However, in further work, it would be interesting to evaluate cell number (DNA content) in hydrogel

Ngày tải lên: 01/11/2022, 09:04

... THEMES IN STEM CELL BANKING 1 Stem Cell Banking: A Global View 3 Glyn Stacey 2 Quality Assurance in Stem Cell Banking: Emphasis on Embryonic and Induced Pluripotent Stem Cell Banking ... control, Stem cell banks, Human embryonic stem cells, Induced pluripotent stem cells 1 Introduction The QA in stem cell banking ensures that the quality of the banked stem cells is in accordance ... upsurge in stem cell research and development (R&D), including technological breakthroughs in creating new types of stem cells such as induced pluripotent stem cells (iPSCs), as well as clinical

Ngày tải lên: 13/05/2017, 21:21

... 1Methods in Molecular Biology Methods in Molecular BiologyTM TMEdited by Anthony P Hollander Paul V Hatton Biopolymer Methods in Tissue Engineering VOLUME 238 Biopolymer Methods in Tissue Engineering ... tested include the murine fibroblast line (3T3), murineleukemic cell line (P-388), human ovarian carcinoma line (OV-1063), murine mam-mary adenocarcinoma cells (EMT-6), and murine macrophage-like cell ... 3T3and mouse mammary carcinoma lines (18,19) FMB minimally attached normal keratinocytes and different cell lines of the leukocytic lineage Cells could bemaintained on FMB in extremely high densities

Ngày tải lên: 10/04/2014, 11:11

... sec-ondary injury, such as apoptotic cell death and inflam-mation [8,9] Figure 4 Tracking of BrdU-labeled neural stem cells at passage six in the ischemic brain of rat having undergone cell therapy ... neural stem cells and Schwann Cells results in functional recovery in a rat spinal cord contusion injury model Biomed Environ Sci 2007, 20:242-249. 12 Lindvall O, Kokaia Z, Martinez-Serrano A: Stem ... using a 10-0 suture After covering the burr hole with a piece of gelatin sponge, the temporalis and overlying skin were sutured Animals were then placed in the supine position and a midline incision

Ngày tải lên: 18/06/2014, 16:20

... stem cells that originate from the inner cell mass of a blastocyst stage embryo The embryonal carcinoma cells replicate and grow in cell culture conditions In 1981, embryonic stem cells (ES cells) ... by cell sorting to minimise the number of pluri/ multipotent stem cells in the cell preparation [86], or to separate tumourigenic stem cells from non-tumourigenic stem cells (by e.g cell sorting ... cultured cells, including stem cells These risk factors are not unique to stem cells and apply to all cell based medicinal products Donor history is of particular importance for stem cell lines which

Ngày tải lên: 18/06/2014, 19:20

... for estimating daily urine volume and measuring the ratio of urine protein to urine creatinine excretion Quantification of urine protein, BUN, and creatinine level was performed using standard ... and creatinine, urine amount, and the ratio of urine protein to creatinine Serum levels of blood urea nitrogen (BUN) and creatinine and the ratio of urine protein to creatinine in three groups ... adiponectin, the anti-inflamma-tory indexes, were notably lower in group 2 than in group 3 (Table 1) These findings imply that ADMSC therapy inhibited inflammatory reaction in this experi-mental setting

Ngày tải lên: 18/06/2014, 19:20

... population of stem/progenitor cells in the breast cancer (MCF7) cell line [11] The fact that the vast majority of MCF7 stem cell enriched SP cells express MUC1 suggests that epithelial cancer stem cells ... cancer stem cells which are capable of maintaining the self-renewal property (Figure 5) Figure 3 Colony formation in MUC4-trasfected cells SKOV3-Vector and SKOV3-MUC4 cells were seeded in equal ... have further shown an increased expression of stem cell regulatory genes, increased invasionin vitro and increased tumori-genesis in NOD/SCID mice [10] This suggests that, in addition to genetic

Ngày tải lên: 20/06/2014, 07:20

... 2iiThis page intentionally left blankTrang 3Numerical Methods in Engineering with PythonSecond Edition Numerical Methods in Engineering with Python, Second Edition, is a text for engineering students ... computer methods, including finite element and boundary el-ement methods, for more than 30 years He is also the co-author of four Engineer-other books – Engineering Mechanics: Statics, Engineering ... the line are ignored by the interpreter Strings A string is a sequence of characters enclosed in single or double quotes Strings are concatenated with the plus (+) operator, whereas slicing (:)

Ngày tải lên: 07/08/2014, 04:20

... number of the matrixUse the functioninv(A)innumpy.linalgto determine the inverse of A. 2.4 Symmetric and Banded Coefficient Matrices Introduction Engineering problems often lead to coefficient ... process involves taking square roots of certain combinations of the elements of A It can be shown that in order to avoid square roots of nega-tive numbers A must be posinega-tive definite Choleski’s ... (2.17) Proceeding to other columns, we observe that the unknown in Eq (2.17) is L ij (the other elements of L appearing in the equation have already been computed) Taking the term containing L ijoutside

Ngày tải lên: 07/08/2014, 04:20

... technologies including fluorescent activated cell sorting and mouse engineering, that give us a more integrated view of the nature of stem cells This book, Advances in Hematopoietic Stem Cell Research, ... through certain intrinsic factors, such as STAT5, which may serve as a component of MPL−induced signaling pathway, mediating MPL’s effects in maintaining HSC quiescence Interestingly, among those ... role in all types of stem cells In some stem cells there is an intrinsic polarity where molecules are segregated along an axis and serves as determinant of cell fate after cell division In these...

Ngày tải lên: 27/06/2014, 13:20

... upon cell division and identified that a proportion of cells were ‘label retaining’ (Cotsarelis et al., 1990) Similar observations were made in other systems containing stem cells, including the ... existing in a population that are negative for staining of CD markers commonly expressed on mature lineage cells, including erythroid, granulocyte, B and T cells (defined from onwards as lineage ... understanding of the regulation of stem cell properties is critical for improving our ability to manipulate normal stem cells in vitro and in vivo Furthermore, understanding normal stem cell regulation...

Ngày tải lên: 22/12/2014, 21:11

... Graduate Program in BioEngineering, investigations of the synthetic compounds were more on their applications in tissue engineering, which is in stem cells differentiation, in this study More ... inhibitors (Aprotinin, Leupeptin, Pepstatin A, PMSF and SOV) were freshly added each time before using Substrate preparation: 1mM D-erythro-sphingosine was prepared by mixing 20μl sphingosine (50mM) ... differently in inhibiting in vitro over-expressed SPHK1 (Figure 2.5A), and in cells endogenous SPHK1 (Figure 2.6) For example, in in vitro over-expressed SPHK1 inhibition test, compounds inhibition...

Ngày tải lên: 11/09/2015, 16:06

... would be convincing that the stem cells remained as stem cells Starting cells for human BM-MSCs were passage 6, and for human AD-MSCs (cells from two different donors) were passage Cells were equally ... S1P in human BM- and AD- MSCs proliferation proliferation, as well as maintaining their stemness However, human adult stem cells, such as MSCs, are very different with embryonic stem cells in ... fixed and stained with Alizarin Red S The Alizarin Red S Staining quantification results are shown in Figure 4.11 Alizarin Red Staining quantification of human BM-MSCs cultured in different conditions...

Ngày tải lên: 11/09/2015, 16:06

... "Signalling mechanisms in sphingosine 1-phosphatepromoted mesangial cell proliferation." Genes Cells 7(12): 1217-30 Kee, T H., P Vit, et al (2005) "Sphingosine kinase signalling in immune cells." Clin ... “Charaterization of N,N-dimethyl-Derythro-sphingosine-induced apoptosis and signaling in U937 cells: Independece of sphingosine kinase inhibition.” Prostaglandins & other lipid Mediators 86: 18-25 Kim, ... “Characterization of N, N-dimethyl-derythro-sphingosine-induced apoptosis and signaling in U937 cells: independence of sphingosine kinase inhibition.” Prostaglandins & Other Lipid Mediators 86(1-4):18-25...

Ngày tải lên: 11/09/2015, 16:06

... be involved in cell proliferation, including in embryonic stem cell proliferation (Pébay et al., 2005) In addition, inhibition of SPHK has been suggested to play a role in mediating embryonic stem ... Therefore, mediating stem cells differentiation, in particular, promoting stem cells differentiation, in a controlled way, would be highly desirable in stem cells research and in their future clinical ... avoids intimate cell- to -cell contact, thus avoiding difficulties in separating co-cultured cells, but still retaining the advantages of fast transduction molecules transmission between culturing stem...

Ngày tải lên: 11/09/2015, 16:06